A. Antinori
Alberto SpanòRelated papers
Apoptotic molecular machinery: Vastly increased complexity in vertebrates revealed by genome comparisons. Aravind L, Dixit VM, Koonin EV.∗ ∗E-mail: koonin@ncbi.nlm.nih.gov Science 2001;291:1279–1284
American Journal of Ophthalmology, 2001
A mimic of HIV-1 nucleocapsid protein impairs reverse transcription and displays antiviral activity
Proceedings of the National Academy of Sciences, 1999
PNAS Plus: From the Cover: IFI16 senses DNA forms of the lentiviral replication cycle and controls HIV-1 replication
Proceedings of the National Academy of Sciences, 2013
A Virion-Associated Protein Kinase Induces Apoptosis
Apoptosis and inhibition of host gene expression are often associated with virus infections. Many viral polypeptides modulate apoptosis by direct interaction with highly conserved apoptotic pathways. Some viruses induce apoptosis during late stages of the infection cycle, while others inhibit apoptosis to facilitate replication or maintain persistent infection. In previous work, we showed that Chilo iridescent virus (CIV) or CIV virion protein extract induces apoptosis in spruce budworm and cotton boll weevil cell cultures. Here, we characterize the product of a CIV gene (iridovirus serine/threonine kinase; istk) with signature sequences for S/T kinase and ATP binding. ISTK appears to belong to the superfamily, vaccinia-related kinases (VRKs). The istk gene was expressed in Pichia pastoris vectors. Purified ISTK (48 kDa) exhibited S/T kinase activity. Treatment with ISTK induced apoptosis in budworm cells. A 35-kDa cleavage product of ISTK retaining key signature sequences was identified during purification. Pichia-expressed 35-kDa polypeptide, designated iridoptin, induced apoptosis and inhibition of host protein synthesis in budworm and boll weevil cells. A mutation in the ATP-binding site eliminated both kinase and apoptosis activity of iridoptin, suggesting that kinase activity is essential for induction of apoptosis. Analysis with custom antibody confirmed that ISTK is a structural component of CIV particles. This is the first demonstration of a viral kinase inducing apoptosis in any virus-host system and the first identification of a factor inducing apoptosis or host protein shutoff for the family Iridoviridae.
Automated Genome-Wide Visual Profiling of Cellular Proteins Involved in HIV Infection
Journal of Biomolecular Screening, 2011
Gateway® Citations
Publications found online in 2005.
(Many thanks to Dr. James Hartley, Director of Protein Expression Laboratory
at NCI / SAI C for putting together the list.)
1. Anna Aagaard, Pawel Listwan, Nathan Cowieson, Thomas Huber,
Timothy Ravasi, Christine A. Wells, Jack U. Flanagan, Stuart Kellie, David
A. Hume, Bostjan Kobe and Jennifer L. Martin. An Inflammatory Role
for the Mammalian Carboxypeptidase Inhibitor Latexin:
Relationship to Cystatins and the Tumor Suppressor TIG1.
Structure Volume 13, I ssue 2, February 2005, Pages 309-317. [ Studier's
autoinduction, pDest17.]
2. I sabella Abbate, Giuseppina Cappiello, Roberta Longo, Antonella Ursitti,
Alberto Spanò, Silvia Calcaterra, Ferdinando Dianzani, Andrea Antinori and
Maria R. Capobianchi. Cell membrane proteins and quasispecies
compartmentalization of CSF and plasma HIV-1 from aids
patients with neurological disorders. I nfection, Genetics and
Evolution Volume 5, I ssue 3, April 2005, Pages 247-253.
3. Nawroz Abdulrazzak, Brigitte Pollet, Jürgen Ehlting, Kim Larsen, Carole
Asnaghi, Sebastien Ronseau, Caroline Proux, Mathieu Erhardt, Virginie
Seltzer, Jean-Pierre Renou, Pascaline Ullmann, Markus Pauly, Catherine
Lapierre, and Danièle Werck-Reichhart. A coumaroyl-ester-3-
hydroxylase Insertion Mutant Reveals the Existence of
Nonredundant meta-Hydroxylation Pathways and Essential Roles
for Phenolic Precursors in Cell Expansion and Plant Growth. Plant
Physiology, Jan 2006; 140: 30 - 48.
4. Brian Abel, Stefan Freigang, Martin F. Bachmann, Ursula Boschert, and
Manfred Kopf. Osteopontin Is Not Required for the Development of
Th1 Responses and Viral Immunity. J. I mmunol., Nov 2005; 175:
6006 - 6013.
5. Chantal Abergel, Sabine Chenivesse, Deborah Byrne, Karsten Suhre,
Vincent Arondel and Jean-Michel Claverie. Mimivirus TyrRS:
preliminary structural and functional characterization of the first
amino-acyl tRNA synthetase found in a virus. Acta Cryst (2005) F61,
212-215.
6. Acton TB, Gunsalus KC, Xiao R, Ma LC, Aramini J, Baran MC, Chiang YW,
Climent T, Cooper B, Denissova NG, Douglas SM, Everett JK, Ho CK,
Macapagal D, Rajan PK, Shastry R, Shih LY, Swapna GV, Wilson M, Wu M,
Gerstein M, I nouye M, Hunt JF, Montelione GT. Robotic cloning and
Protein Production Platform of the Northeast Structural
Genomics Consortium. Methods Enzymol. 2005;394:210-43.
7. Sung-Hee Ahn, Wang L. Cheung, Jer-Yuan Hsu, Robert L. Diaz, M.
Mitchell Smith, and C. David Allis. Sterile 20 Kinase Phosphorylates
Histone H2B at Serine 10 during Hydrogen Peroxide-Induced
Apoptosis in S. cerevisiae. Cell 2005 120: 25-36.
8. Jihui Ai, Paula Smith, Shuwei Wang, Ping Zhang, and X. Long Zheng. The
proximal carboxyl terminal domains of ADAMTS13 determine
substrate specificity and are all required for cleavage of Von
Willebrand factor. J. Biol. Chem., Jun 2005; 10.1074/ jbc.M505513200.
9. Malika Akermoun, Markus Koglin, Darina Zvalova-I ooss, Nicolas
Folschweiller, Simon J. Dowell and Katy L. Gearing. Characterization of
16 human G protein-coupled receptors expressed in baculovirusinfected insect cells. Protein Expression and Purification, Volume 44,
I ssue 1, November 2005, Pages 65-74
10. Michael Albers, Beatrix Blume, Thomas Schlueter, Matthew B. Wright,
I ngo Kober, Claus Kremoser, Ulrich Deuschle, and Manfred Koegl. A
novel principle for partial agonism of LXR ligands: Competitive
recruitment of activators and repressors. J. Biol. Chem., Dec 2005;
10.1074/ jbc.M510101200. [ Yeast 2 hybrid using GW versions of ClonTech
vectors]
11. Michael Albers, Harald Kranz, I ngo Kober, Carmen Kaiser, Martin Klink,
Jörg Suckow, Rainer Kern, and Manfred Koegl. Automated Yeast Twohybrid Screening for Nuclear Receptor-interacting Proteins. Mol.
Cell. Proteomics, Feb 2005; 4: 205 - 213.
12. Béatrice Alpha-Bazin, Alain Lorphelin, Nathalie Nozerand, Gaëlle Charier,
Charles Marchetti, Frédéric Bérenguer, Joël Couprie, Bernard Gilquin,
Sophie Zinn-Justin, and Eric Quéméneur. Boundaries and physical
characterization of a new domain shared between mammalian
53BP1 and yeast Rad9 checkpoint proteins. Protein Sci., Jul 2005;
14: 1827 - 1839.
13. T. Altenberger, M. Bilban, M. Auer, E. Knosp, S. Wolfsberger, W. Gartner,
I . Mineva, C. Zielinski, L. Wagner and A. Luger. Identification of DLK1
variants in pituitary- and neuroendocrine tumors. Biochemical and
Biophysical Research Communications, I n Press, Uncorrected Proof,
Available online 27 December 2005.
14. Thorsten Althoff, Hartmut Hentschel, Jutta Luig, Hendrike Schutz,
Myriam Kasch, and Rolf K.-H. Kinne. Na+-D-glucose cotransporter in
the kidney of Squalus acanthias: molecular identification and
intrarenal distribution. Am J Physiol Regulatory I ntegrative Comp
Physiol, Nov 2005; 10.1152/ ajpregu.00334.2005
15. Marco Antonio Alvarez-Pérez, Sampath Narayanan, Margarita ZeichnerDavid, Bruno Rodríguez Carmona and Higinio Arzate. Molecular cloning,
expression and immunolocalization of a novel human cementumderived protein (CP-23). Bone, I n Press, Corrected Proof, Available
online 2 November 2005
16. Gualtiero Alvisi, David A. Jans, Jinjin Guo, Lorenzo A. Pinna, Alessandro
Ripalti. A Protein Kinase CK2 Site Flanking the Nuclear Targeting
Signal Enhances Nuclear Transport of Human Cytomegalovirus
ppUL44. Traffic Volume 6, I ssue 11, Page 1002-1013, Nov 2005
17. Anthony Antonellis, William R. Bennett, Trevelyan R. Menheniott, Arjun
B. Prasad, Shih-Queen Lee-Lin, NI SC Comparative Sequencing Program,
Eric D. Green, Derek Paisley, Robert N. Kelsh, William J. Pavan, and
Andrew Ward . Deletion of Long-Range Sequences at Sox10
Compromises Developmental Expression in a Mouse Model of
Waardenburg-Shah (WS4) Syndrome. Hum. Mol. Genet., 2006;
15(2): p. 259-271
18. Satoko Arai and Toru Miyazaki. Impaired maturation of myeloid
progenitors in mice lacking novel Polycomb group protein MBT-1.
EMBO J., May 2005; 10.1038/ sj.emboj.7600654.
[ AdenoV]
19. Romulo Aráoz, Nadine Honoré, Sungae Cho, Jong-Pill Kim, Sang-Nae Cho,
Marc Monot, Caroline Demangel, Patrick J. Brennan, and Stewart T. Cole.
Antigen Discovery: a Postgenomic Approach to Leprosy Diagnosis.
I nfect. I mmun., Jan 2006; 74: 175 - 182.
[ Leprosy]
20. Emily E. Arias, Johannes C. Walter. PCNA functions as a molecular
platform to trigger Cdt1 destruction and prevent re-replication.
Nature Cell Biology (18 Dec 2005)
21. Dorit Arlt, Wolfgang Huber, Urban Liebel, Christian Schmidt, Meher
Majety, Mamatha Sauermann, Heiko Rosenfelder, Stephanie Bechtel,
Alexander Mehrle, Detlev Bannasch, I ngo Schupp, Markus Seiler, Jeremy C.
Simpson, Florian Hahne, Petra Moosmayer, Markus Ruschhaupt, Birgit
Guilleaume, Ruth Wellenreuther, Rainer Pepperkok, Holger Sültmann,
Annemarie Poustka, and Stefan Wiemann. Functional Profiling: From
Microarrays via Cell-Based Assays to Novel Tumor Relevant
Modulators of the Cell Cycle. Cancer Res., Sep 2005; 65: 7733 - 7742.
[ Wiemann, Ca genes localized w FP fusions.]
22. Arma, Nurrahmawaty, Hirono, I kuo, Aoki, Takashi. Identification of
genes expressed in the liver of Japanese flounder Paralichthys
olivaceus by expressed sequence tags. Fisheries Science, Volume 71,
Number 3, June 2005, pp. 504-518(15).
23. Sylvain Arnould, Patrick Chames, Christophe Perez, Emmanuel Lacroix,
Aymeric Duclert, Jean-Charles Epinat, François Stricher, Anne-Sophie Petit,
Amélie Patin, Sophie Guillier, Sandra Rolland, Jesús Prieto, Francisco J.
Blanco, Jerónimo Bravo, Guillermo Montoya, Luis Serrano, Philippe
Duchateau and Frédéric Pâques. Engineering of Large Numbers of
Highly Specific Homing Endonucleases that Induce
Recombination on Novel DNA Targets. Journal of Molecular Biology,
I n Press, Uncorrected Proof, Available online 11 November 2005.
24. Maximiliano Juri Ayub, Cristian R. Smulski, Benson Nyambega, Natalia
Bercovich, Dan Masiga, Martín P. Vazquez, Carlos F. Aguilar and Mariano J.
Levin. Protein–protein interaction map of the Trypanosoma cruzi
ribosomal P protein complex. Gene, I n Press, Corrected Proof,
Available online 24 August 2005. [ Yeast 2 hybrid]
25. Taeok Bae and Olaf Schneewind. Allelic replacement in
Staphylococcus aureus with inducible counter-selection. Plasmid,
I n Press, Volume 55, I ssue 1, January 2006, Pages 58-63
26. Mark A. Baker, Anton Krutskikh, Benjamin J. Curry, Louise Hetherington,
and R. John Aitken. Identification of Cytochrome-b5 Reductase as
the Enzyme Responsible for NADH-Dependent Lucigenin
Chemiluminescence in Human Spermatozoa. Biol Reprod, Aug 2005;
73: 334 - 342.
27. Jana Baranova, Eva Anzenbacherova, Pavel Anzenbacher, and Pavel
Soucek. Minipig cytochrome P450 2E1: comparison with human
enzyme. Drug Metab. Dispos., 2005 Jun;33(6):862-5.
28. Dipanwita Basu, Jie Le, Salah El-Din El-Essal, Shanjin Huang, Chunhua
Zhang, Eileen L. Mallery, Gregore Koliantz, Christopher J. Staiger, and
Daniel B. Szymanski. DISTORTED3/SCAR2 Is a Putative Arabidopsis
WAVE Complex Subunit That Activates the Arp2/3 Complex and
Is Required for Epidermal Morphogenesis. PLANT CELL, Feb 2005;
17: 502 - 524.
29. Sébastien Baud, Sylvie Wuillème, Rémi Lemoine, Jocelyne Kronenberger,
Michel Caboche, Loïc Lepiniec, Christine Rochat. The AtSUC5 sucrose
transporter specifically expressed in the endosperm is involved in
early seed development in Arabidopsis. The Plant Journal Volume 43,
I ssue 6, Page 824-836, Sep 20054.
30. Wiebke Baum, Vladimir Kirkin, Sara B. Mateus Fernández, Robert Pick,
Marcus Lettau, Ottmar Janssen, and Martin Zörnig. Binding of the
intracellular Fas ligand (FasL) domain to the adaptor protein
PSTPIP results in a cytoplasmic localization of FasL. J. Biol. Chem.,
Dec 2005; 280: 40012 - 40024
31. Jake Baum, Dave Richard, Julie Healer, Melanie Rug, Zita Krnajski, TimWolf Gilberger, Judith L. Green, Anthony A. Holder, and Alan F. Cowman.
A conserved molecular motor drives cell invasion and gliding
motility across malaria lifecycle stages and other apicomplexan
parasites. J. Biol. Chem., Dec 2005; 10.1074/ jbc.M509807200. [ Multisite,
for expressing C-tagged Plasmodium proteins in parasites.]
32. Lone Baunsgaard, Henrik Lütken, René Mikkelsen, Mikkel A. Glaring,
Tam T. Pham and Andreas Blennow. A novel isoform of glucan, water
dikinase phosphorylates pre-phosphorylated -glucans and is
involved in starch degradation in Arabidopsis. The Plant Journal,
Volume 41, I ssue 4, Page 595-605, February 2005.
33. Pascale Beffy, Cristina Barsanti, Renata Del Carratore, Silvana Simi, Pier
Alberto Benedetti, Luca Benzi, Alessandro Prelle, Patrizia Ciscato and
Marcella Simili. Expression and localization of myotonic dystrophy
protein kinase in human skeletal muscle cells determined with a
novel antibody: possible role of the protein in cytoskeleton
rearrangements during differentiation. Cell Biology I nternational, I n
Press, Corrected Proof, Available online 11 August 2005.
34. Alexandra Beilina, Marcel Van Der Brug, Rili Ahmad, Sashi Kesavapany,
David W. Miller, Gregory A. Petsko, and Mark R. Cookson. Mutations in
PTEN-induced putative kinase 1 associated with recessive
parkinsonism have differential effects on protein stability. PNAS,
Apr 2005; 10.1073/ pnas.0500617102.
35. Bell JK, Botos I , Hall PR, Askins J, Shiloach J, Segal DM, Davies DR. The
molecular structure of the Toll-like receptor 3 ligand-binding
domain. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10976-80.
[ Gateway not mentioned but definitely used.]
36. Bembenek ME, Kuhn E, Mallender WD, Pullen L, Li P, Parsons T. A
fluorescence-based coupling reaction for monitoring the activity
of recombinant human NAD synthetase. Assay Drug Dev Technol.
2005 Oct; 3(5):533-41.
37. Sandra Bensmihen, Jérôme Giraudat, and François Parcy.
Characterization of three homologous basic leucine zipper
transcription factors (bZIP) of the ABI5 family during Arabidopsis
thaliana embryo maturation. J. Exp. Bot., Jan 2005;
10.1093/ jxb/ eri050.
38. Jennifer Berbaum and Richard K. Harrison. Comparison of full-length
versus ligand binding domain constructs in cell-free and cellbased peroxisome proliferator-activated receptor alpha assays.
Analytical Biochemistry, I n Press, Uncorrected Proof, Available online 1
February 2005.
39. Adriana J Bernal, Qilin Pan, Jeff Pollack, Laura Rose, Alexander Kozik,
Neil Willits, Yao Luo, Muriel Guittet, Elena Kochetkova, and Richard
Michelmore. Functional analysis of the plant disease resistance
gene Pto using DNA shuffling. J. Biol. Chem., Mar 2005; 10.1074/
jbc.M500992200.
40. Nina K. Bernstein, R. Scott Williams, Melissa L. Rakovszky, Diana Cui,
Ruth Green, Feridoun Karimi-Busheri, Rajam S. Mani, Sarah Galicia, C.
Anne Koch, Carol E. Cass, Daniel Durocher, Michael Weinfeld and J.N.
Mark Glover. The Molecular Architecture of the Mammalian DNA
Repair Enzyme, Polynucleotide Kinase. Molecular Cell, Volume 17,
I ssue 5, 4 March 2005, Pages 657-670.
41. Paul Bertone and Michael Snyder. Advances in functional protein
microarray technology. FEBS J., Nov 2005; 272: 5400 - 5411. [ Protein
microarrays review: "By far the greatest obstacle in developing functional
protein microarrays is the construction of a comprehensive expression
clone library"
42. Sonja M. Best, Keely L. Morris, Jeffrey G. Shannon, Shelly J. Robertson,
Dana N. Mitzel, Gregory S. Park, Elena Boer, James B. Wolfinbarger, and
Marshall E. Bloom. Inhibition of Interferon-Stimulated JAK-STAT
Signaling by a Tick-Borne Flavivirus and Identification of NS5 as
an Interferon Antagonist. J. Virol., Oct 2005; 79: 12828 - 12839.
43. Michael Bevan and Sean Walsh. The Arabidopsis genome: A
foundation for plant research. Genome Res., Dec 2005; 15: 1632 1642. [ Review]
44. Bezanilla M, Perroud PF, Pan A, Klueh P, Quatrano RS. An RNAi
System in Physcomitrella patens with an Internal Marker for
Silencing Allows for Rapid Identification of Loss of Function
Phenotypes. Plant Biol (Stuttg). 2005 May;7(3):251-7.
45. Riyaz A. Bhat, Marco Miklis, Elmon Schmelzer, Paul Schulze-Lefert, and
Ralph Panstruga. Recruitment and interaction dynamics of plant
penetration resistance components in a plasma membrane
microdomain. PNAS, Feb 2005; 102: 3135 - 3140.
46. Amit P. Bhavsar, Ray Truant, and Eric D. Brown. The TagB protein in
bacillus subtilis 168 is an intracellular peripheral membrane
protein that can incorporate glycerol-phosphate onto a
membrane bound acceptor in vitro. J. Biol. Chem., Sep 2005;
10.1074/ jbc.M507154200.
47. Elsa P. Bianchini, Steven J. Orcutt, Peter Panizzi, Paul E. Bock, and
Sriram Krishnaswamy. Ratcheting of the substrate from the
zymogen to proteinase conformations directs the sequential
cleavage of prothrombin by prothrombinase. PNAS, Jul 2005;
10.1073/ pnas.0504704102.
48. Emanuele G. Biondi, Jeffrey M. Skerker, Muhammad Arif, Melanie S.
Prasol, Barrett S. Perchuk and Michael T. Laub. A phosphorelay
system controls stalk biogenesis during cell cycle progression in
Caulobacter crescentus. Molecular Microbiology Volume 59 Page 386
- January 2006
49. Biswal BK, Cherney MM, Wang M, Garen C, James MN. Structures of
Mycobacterium tuberculosispyridoxine 5'-phosphate oxidase and
its complexes with flavin mononucleotide and pyridoxal 5'phosphate. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt
11):1492-9. Epub 2005 Oct [ GST fusion w TEV site]
50. Geir Bjorkoy, Trond Lamark, Andreas Brech, Heidi Outzen, Maria
Perander, Aud Overvatn, Harald Stenmark, and Terje Johansen.
p62/SQSTM1 forms protein aggregates degraded by autophagy
and has a protective effect on huntingtin-induced cell death. J.
Cell Biol. published 14 November 2005, 10.1083 / jcb.200507002
[ Huntingtin;> 10 GW clones]
51. Paul G. Blommel, Peter A. Martin, Russell L. Wrobel, Eric Steffen and
Brian G. Fox. High efficiency single step production of expression
plasmids from cDNA clones using the Flexi Vector cloning system.
Protein Expression and Purification, I n Press, Uncorrected Proof, Available
online 5 December 2005 [ FlexiVector from Promega]
52. Suzanne S. Bohlson, Richard Silva, Maria I . Fonseca, and Andrea J.
Tenner.. CD93 Is Rapidly Shed from the Surface of Human Myeloid
Cells and the Soluble Form Is Detected in Human Plasma. J.
I mmunol., Jul 2005; 175: 1239 - 1247
53. Stefania Bonsegna, Stephen P. Slocombe, Luigi De Bellis and Alison
Baker. AtLACS7 interacts with the TPR domains of the PTS1
receptor PEX5. Archives of Biochemistry and Biophysics, I n Press,
Uncorrected Proof, Available online 7 October 2005
54. Frédéric Bornancin, Diana Mechtcheriakova, Samantha Stora, Christine
Graf, Alexander Wlachos, Piroska Dévay, Nicole Urtz, Thomas Baumruker
and Andreas Billich. Characterization of a ceramide kinase-like
protein. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology
of Lipids, Volume 1687, I ssues 1-3, 21 February 2005, Pages 31-43.
55. I stvan Botos, Edward E. Melnikov, Scott Cherry, Serguei Kozlov, Oksana
V. Makhovskaya, Joseph E. Tropea, Alla Gustchina, Tatyana V. Rotanova
and Alexander Wlodawer. Atomic-resolution Crystal Structure of the
Proteolytic Domain of Archaeoglobus fulgidus Lon Reveals the
Conformational Variability in the Active Sites of Lon Proteases.
Journal of Molecular Biology, Volume 351, I ssue 1, 5 August 2005, Pages
144-157
56. Jean-Marie Bourhis, Véronique Receveur-Bréchot, Michael Oglesbee,
Xinsheng Zhang, Matthew Buccellato, Hervé Darbon, Bruno Canard,
Stéphanie Finet, and Sonia Longhi. The intrinsically disordered C-
terminal domain of the measles virus nucleoprotein interacts
with the C-terminal domain of the phosphoprotein via two
distinct sites and remains predominantly unfolded. Protein Sci.,
Aug 2005; 14: 1975 - 1992.
57. Yann Boursiac, Sheng Chen, Doan-Trung Luu, Mathias Sorieul, Niels van
den Dries, and Christophe Maurel. Early Effects of Salinity on Water
Transport in Arabidopsis Roots. Molecular and Cellular Features
of Aquaporin. Expression Plant Physiology, Sep 2005; 10.1104
/ pp.105.065029.
58. Andreas Brachner, Siegfried Reipert, Roland Foisner, and Josef
Gotzmann. LEM2 is a novel MAN1-related inner nuclear membrane
protein associated with A-type lamins. J. Cell Sci., Dec 2005; 118:
5797 - 5810.
59. Anders Bratt, Olivier Birot, I ndranil Sinha, Niina Veitonmäki, Karin Aase,
Mira Ernkvist, and Lars Holmgren. Angiomotin regulates endothelial
cell-cell junctions and cell motility. J. Biol. Chem., Jul 2005; 10.1074
/ jbc.M503915200.
60. Braud, S.; Moutiez, M.; Belin, P.; Abello, N.; Drevet, P.; Zinn-Justin, S.;
Courcon, M.; Masson, C.; Dassa, J.; Charbonnier, J.-B.; Boulain, J.-C.;
Menez, A.; Genet, R.; Gondry, M.. Dual Expression System Suitable
for High-Throughput Fluorescence-Based Screening and
Production of Soluble Proteins. J. Proteome Res.; (Article); 2005;
ASAP Article; DOI : 10.1021/ pr050230i
61. Brecht K, Simonen M, Kamke M, Heim J. Hematopoietic transcription
factor GATA-2 promotes upregulation of alpha globin and cell
death in FL5.12 cells. Apoptosis. 2005 Oct;10(5):1063-78. [ RetroV]
62. Brecht K, Simonen M, Heim J. Upregulation of alpha globin
promotes apoptotic cell death in the hematopoietic cell line
FL5.12. Apoptosis. 2005 Oct;10(5):1043-62. [ RetroV]
63. Thomas Brettin, Michael R Altherr, Ying Du, Roxie M Mason, Alexandra
Friedrich, Laura Potter, Chris Langford, Thomas J Keller, Jason Jens,
Heather Howie, Nathan J Weyand, Susan Clary, Kimberly Prichard, Susi
Wachocki, Erica Sodergren, Joseph P Dillard, George Weinstock,
Magdalene So, and Cindy G Arvidson. Expression capable library for
studies of Neisseria gonorrhoeae, version 1.0. BMC Microbiol, Sep
2005; 5(1): 50.
[ N. Gonorrhoeae ORFeome.]
64. Philip A. Bronstein, Matthew Marrichi, Sam Cartinhour, David J.
Schneider, and Matthew P. DeLisa. Identification of a Twin-Arginine
Translocation System in Pseudomonas syringae pv. tomato
DC3000 and Its Contribution to Pathogenicity and Fitness. J.
Bacteriol., Dec 2005; 187: 8450 - 8461.
65. Vladimir Brukhin, Jacqueline Gheyeselinck, Valeria Gagliardini, Pascal
Genschik, and Ueli Grossniklaus. The RPN1 Subunit of the 26S
Proteasome in Arabidopsis Is Essential for Embryogenesis. PLANT
CELL, Sep 2005; 10.1105/ tpc.105.034975.
66. Jack-Ansgar Bruun, Ernst I van Simon Thomassen, Kurt Kristiansen, Garth
Tylden, Turid Holm, I ngvild Mikkola, Geir Bjørkøy, and Terje Johansen.
The third helix of the homeodomain of paired class homeodomain
proteins acts as a recognition helix both for DNA and protein
interactions. Nucleic Acids Res., May 2005; 33: 2661 - 2675.
[ Nterminal YFP and C-terminal CFP destination vectors.]
67. Lukas Burkle, Svenja Meyer, Hakan Dortay, Hans Lehrach, and Alexander
Heyl. In vitro recombination cloning of entire cDNA libraries in
Arabidopsis thaliana and its application to the yeast two-hybrid
system. Funct I ntegr Genomics, Feb 2005. To monitor for a size bias
generally assumed to be inherent to in vitro recombination methods, the
libraries were characterized before and after the transfer into the
expression vector. However no significant difference could be detected.
68. Didier Busso, Bénédicte Delagoutte-Busso and Dino Moras.
Construction of a set Gateway-based destination vectors for
high-throughput cloning and expression screening in Escherichia
coli. Analytical Biochemistry, I n Press, Uncorrected Proof, Available online
9 June 2005.
69. Busso D, Poussin-Courmontagne P, Rose D, Ripp R, Litt A, Thierry JC,
Moras D. Structural Genomics of Eukaryotic Targets at a
Laboratory Scale. J Struct Funct Genomics. 2005 Sep;6(2-3):81-88.
70. Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork P,
Lehrach H, Heinemann U. Structural genomics of human proteins--
target selection and generation of a public catalogue of
expression clones. Microb Cell Fact. 2005 Jul 5;4:21.
[ Decided not
to use GW, too many extra amino acids]
71. Luz I .A. Calderon-Villalobos, Carola Kuhnle, Esther M.N. Dohmann,
Hanbing Li, Mike Bevan, and Claus Schwechheimer. The Evolutionarily
Conserved TOUGH Protein Is Required for Proper Development of
Arabidopsis thaliana. PLANT CELL, Jul 2005; 10.1105/ tpc.105.031302.
72. Cali L, Wang B, Mikhail M, Gill MJ, Beckthold B, Salemi M, Jans DA, Piller
SC, Saksena NK. Evidence for host-driven selection of the HIV type
1 vpr gene in vivo during HIV disease progression in a
transfusion-acquired cohort. AI DS Res Hum Retroviruses. 2005
Aug;21(8):728-33.
73. Maureen Caligiuri, Frank Becker, Krishna Murthi, Faith Kaplan, Severine
Dedier, Christine Kaufmann, Andy Machl, Gabriele Zybarth, Judson
Richard, Nick Bockovich, Art Kluge, and Nikolai Kley A Proteome-Wide
CDK/CRK-Specific Kinase Inhibitor Promotes Tumor Cell Death in
the Absence of Cell Cycle Progression.
Chemistry and Biology
2005 12: 1103-1115.
[ Expression of kinase cDNAs]
74. C. L. Campbell, K. A. Vandyke, G. J. Letchworth, B. S. Drolet, T.
Hanekamp and W. C. Wilson. Midgut and salivary gland
transcriptomes of the arbovirus vector Culicoides sonorensis
(Diptera: Ceratopogonidae). I nsect Molecular Biology Volume 14,
I ssue 2, Page 121-136, April 2005.
75. Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni,
Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian
Cambillau and Yves Bourne. Crystal structure of the conserved
hypothetical protein Rv1155 from Mycobacterium tuberculosis.
FEBS Letters Volume 579, I ssue 1 , 3 January 2005, Pages 215-221.
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