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View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Repositório Científico da Universidade de Évora Bacterial communities associated with Monochamus galloprovincialis, the insect-vector of pine wilt disease C. Vicente, F. X. Nascimento, M. Espada, P. Barbosa, K. Hasegawa, A. Correia, M. Mota INIAV – Instituto Nacional de Investigação Agrária e Veterinária, Quinta do marquês, Oeiras, Portugal. ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia , Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Évora, Portugal Department of Environmental Biology, College of Biosciences and Biotechnology, Chubu University, 1200, Matsumoto, Kasugai, 487-8501, Japan Email:cvicente@uevora.pt Bacterial communities associated with the Bursaphelenchus xylophilus (pine wood nematode, PWN) are suggested to play a role in pine wilt disease (PWD) development. However, it’s not clear where the PWN acquires these communities. In this sense, it is possible that bacterial communities colonizing Monochamus spp. may affect the bacterial communities associated with the PWN. In this work, we present the characterization of bacterial communities of the Portuguese insect vector Monochamus galloprovincialis using culture independent methods, and investigate the common bacterial communities between the insect-vector and the pathogenic agent, PWN. Monochamus galloprovincialis is mainly composed by γ-proteobacteria, Firmicutes and Bacteroidetes, sharing common bacterial genera with B. xylophilus (i.e. Serratia, Bacillus, and Pseudomonas). These results can bring new insights into the role of the insect vector in the PWN-bacteria interaction.