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2003, Chemical Industry
The bioluminescent bioreporter Pseudomonas fluorescens HK44, the whole cell bacterial biosensor that responds to naphthalene and its metabolites via the production of visible light, was immobilized into a silica matrix by the sol-gel technique. The bioluminescence intensities were measured in the maximum of the bioluminescence band at X = 500 nm. The immobilized cells (>105 cells per g silica matrix) produced light after induction by salicylate (cone. > 10 g/l), naphthalene and aminobenzoic acid. The bioluminescence intensities induced by 2,3-dihydroxynaphthalene 3-hydroxybenzoic acid and 4-hydroxybenzoic acid were comparable to a negative control. The cells in the silica layers on glass slides produced light in response to the presence of an inductor at least 8 months after immobilization, and >50 induction cycles. The results showed that these test slides could be used as assays for the multiple determination of water pollution.
Ecological Indicators
Bioluminescent bioreporter Pseudomonas putida TVA8 as a detector of water pollution. Operational conditions and selectivity of free cells sensor2011 •
Applied Microbiology and Biotechnology
Bioluminescence induction response and survival of the bioreporter bacterium Pseudomonas fluorescens HK44 in nutrient-deprived conditions1997 •
2007 •
a Faculty of the Environment, Jan Evangelista Purkyně University in Usti nad Labem, Kralova Výina 3132/7, 400 96 Usti nad Labem, b Institute of Inorganic Chemistry, vvi, Academy of Sciences of the Czech Republic, 250 68 Ře-Husinec 1001, c Institute of Chemical Process Fundamentals, vvi, Academy of Sciences of the Czech Republic, Rozvojova 135, 165 02 Prague 6, Czech Republic, d Department of Inorganic and Organic Chemistry, Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Heyrovskeho 1203, 500 05 Hradec Kralove, e Department of Organic Chemistry, Faculty of Chemical Technology, University of Pardubice, nam. Cs. legii 565, 532 10 Pardubice, f Institute of Chemical Technology, Department of Biochemistry and Microbiology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic, g Center for Environmental Biotechnology, 676 Dabney Hall, University of Tennessee Knoxville, Tennessee 37996, USA trogl@fzp.ujep.cz
Folia Microbiologica
Bioluminescence of Pseudomonas fluorescens HK44 in the course of encapsulation into silica gel. Effect of methanol2010 •
The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)4. The non-induced BLM of free cells was increased in the presence of 0.5–2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1–3 and 8–18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2–6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.
Sensors (Basel, Switzerland)
Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring2017 •
Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in t...
2016 •
Dissertationes Archaeologicae
The fort of Ad Mures (Ács, Komárom-Esztergom County, Hungary) New investigations on the northern section of the ripa Pannonica2023 •
The Roman auxiliary fort of Ad Mures (Ács-Bumbumkút, Komárom-Esztergom County, Hungary) has recently been studied intensively using geophysical surveys and a planned excavation by the team of the Institute of Archaeological Sciences of the Eötvös Loránd University. The paper presents the preliminary results of these investigations, focused on the layout of the fort and the relative chronology of the site, based on the data collected during the 2023 excavation. Results indicate several analogies with the fort of Ad Statuas (Ács-Vaspuszta).
2022 •
Introduction to the temporary exhibition in the new museum of Aigai
Debating evolution in context – religion in the biology classroom – Brazil, England and the United States.
Debating Evolution in Context -Religion in the Biology Classroom2023 •
A. Lahav, En defensa de la litigación (Palestra ed., trad. de Carla Lucero Tarifa Dianderas)
Prefacio a la traducción al español2024 •
Revista de História da Sociedade e da Cultura
Las ‘coutadas’ de Almeirim: poder real y administración del territorio desde una perspectiva histórico-geográfica en Portugal (c. 1560-1650)2022 •
Innsyn 2014, nr. 50.
Uimotståelig nåde- udødelig tro: Presentasjon og vurdering av to kalvinske dogmer.2014 •
2020 •
Arabian Journal of Geosciences
GIS-based DRASTIC and modified DRASTIC techniques for assessing groundwater vulnerability to pollution in Torghabeh-Shandiz of Khorasan County, Iran2020 •
2015 •
Journal of Liaquat University of Medical & Health Sciences
Outcome of Ileostomy in the Management of Ileal Perforation2008 •
The Dhaka Univeristy Journal of Linguistics
From Crises to Opportunities in Higher Education in BangladeshSocial Science Research Network
The Right to Silence Helps the Innocent: A Game-Theoretic Analysis of the Fifth Amendment Privilege2001 •
17. YÜZYIL TOPKAPI SARAYI ÇİNİ PANOLARINDA YER ALAN ÇİÇEK MOTİFLERİ
Yıl: 3, Sayı: 9, Eylül 2017, s. 378-3882017 •
Josef Trögl