Rodent control tends to involve methods that cause animal suffering, but little attention has bee... more Rodent control tends to involve methods that cause animal suffering, but little attention has been paid to the animal welfare implications of rodent control. The aim of the current study was to gain insight into the opinions and attitudes of clients of Dutch pest controllers, regarding liminal rodents, rodent control, and rodent welfare. A better understanding of their attitudes may contribute to more ethical rodent management programmes. An online survey among 248 clients of Dutch pest controllers was carried out. Respondents, especially those within the agricultural sector, have a relatively negative attitude towards rats and mice. Respondents in the agricultural subgroup do not consider the welfare of liminal rodents important. They also think that the welfare impact of commonly used control methods is limited, and they have low tolerance levels for the presence of rodents. Respondents from other sectors have a far more positive attitude towards rats and mice, consider their welf...
To examine the dog ownership factors characteristic to a population of dogs confiscated after a h... more To examine the dog ownership factors characteristic to a population of dogs confiscated after a human and/ or animal-directed biting incident, we compared bite risk assessment reports of 159 confiscated dogs in the time frame 2008, 2009, 2010 (tf1) and of 215 confiscated dogs in the time frame 2020, 2021, 2022 (until mid-May; tf2). The reports were compiled by the same institute in a standardized format. We studied frequencies and chi-square pairwise comparisons (P<0.05) for 30 identified ownership factors. Overall (tf1 and tf2), 1,308 ownership factors were mentioned in the reports and reports mentioning ≥5 factors were twice as frequent in tf2 (38%) than tf1 (16%). Our data suggest that nine factors may in particular serve as a warning signal for biting incidents, as these factors were most frequently (≥15%) prevalent in the total of reported cases: having a multiple dog household, a dog reportedly roaming a neighbourhood without an owner, a dog’s care tasks being transferred, ...
Shelter dogs are exposed to a variety of stressors. Among non-invasive techniques, hair cortisol ... more Shelter dogs are exposed to a variety of stressors. Among non-invasive techniques, hair cortisol concentration (HCC) is suggested an easy to collect biomarker for giving insight into long-term stress responses. We evaluated HCC as an indicator of long-term cortisol responses in dogs in an animal shelter over different chronological time points during sheltering and after adoption. Hair samples were collected from the neck region following a shave/re-shave protocol of shelter dogs (total n = 52) at four different time periods: T1 intake at shelter (pre-shelter period, n = 51); T2 after 6 weeks in the shelter (n = 23); T3 6 weeks after adoption (n = 24); T4 6 months after adoption (n = 22). HCC at T2 was significantly higher than HCC at T1, T3 and T4 (effect of sample collection moment: F3,41 = 12.78, p < 0.0001). The dog’s weight class, age class, sex, reason for admission, kennel history and melanin type also explained HCC variability. No significant difference in HCC was found b...
Abstract Shelter dogs face the challenge of adapting to a kennel environment. Individual differen... more Abstract Shelter dogs face the challenge of adapting to a kennel environment. Individual differences in adaptation are known to exist. Resting patterns might be indicative of adaptability to such a novel environment, but need to be evaluated for its usefulness, like every potentially physiological and behavioural parameter. Here, we evaluated nocturnal activity patterns of dogs as indicators of adaptability to novel environments. We measured nocturnal activity (3-axial accelerometer, the Actical®) and two physiological stress parameters, i.e. urinary cortisol/creatinine ratio (UCCR) and body weight in 29 dogs relinquished to a shelter (SD group) in the first two days after intake at the shelter (n = 29), after a 12-day habituation period in the shelter (n = 28) and >6 weeks post-adoption (n = 17). A control group of 29 pet dogs kept at home (CPD group), matching the SD group characteristics, was also assessed for its nocturnal activity and UCCR. Linear mixed model analysis, t-tests and Friedman tests were used to analyse the data. The main findings are: 1) the SD group exhibited higher nocturnal activity (total activity counts, activity duration and number of rest bouts) the first two nights after intake than on night 12, with decreasing inter-individual variances. Compared to the CPD group they showed higher nocturnal activity on night 1 (all p In conclusion, nocturnal activity, as measured by an accelerometer, may be a valid parameter to monitor adaptability of dogs to a kennel environment. Monitoring nocturnal activity in this way can be a useful and cost-effective additional indicator for assessing dog welfare.
Journal of The American Association for Laboratory Animal Science, Sep 1, 2017
There is a strong need to gain systematic knowledge about the potential impact of routine procedu... more There is a strong need to gain systematic knowledge about the potential impact of routine procedures on laboratory animals to ensure animal welfare and reliability of experimental results. Blood sampling is a frequently used procedure within laboratory animal research. Two of the most common anesthesia-free techniques to obtain blood in mice are the saphenous venipuncture and submandibular bleeding. It is common practice that these methods are applied repeatedly to 1 individual. The impact of both techniques, either performed singly or repeatedly, on the animal’s welfare is not fully known yet. We directly compared the effects of both sampling methods on physiological stress responses, behavior, tissue damage, and procedure duration in 2 frequently used mouse strains: C57BL/6 and BALB/c mice (n = 45/strain). The mice were randomly assigned to 3 treatment groups (saphenous venipuncture, submandibular bleeding, control) of 15 mice/strain and were sampled weekly (80 μl/ blood sample) during 4 wk for the experimental groups and 5 wk for the control group. To evaluate the acute physiological stress response, plasma corticosterone levels were determined. As an indicator of chronic stress, body weights were recorded once a week and the thymus was weighed after necropsy. Behavior was recorded using video cameras in front of the cages and afterwards analyzed by a well-trained observer using event logging software. Tissue damage was evaluated by scoring pre-and postmortem hemorrhages and hematomas at the injection site and by measuring alanine transaminase, aspartate aminotransferase, and creatine kinase plasma levels. Submandibular bleeding appeared to be less time consuming (P<0.05) while providing higher sample volumes. However, this method led to increased acute stress responses in C57BL/6 mice (P = 0.03). All animals of both strains undergoing this procedure expressed more pain-related behavior (P < 0.05) and the extent of tissue damage at the injection site was significantly higher when compared to the saphenous venipuncture (P < 0.05). Thus, in order to avoid the undesirable effects of stress on experimental outcomes and to reduce the extent of tissue damage, saphenous venipuncture should be preferred above submandibular bleeding whenever possible.
Rodent control tends to involve methods that cause animal suffering, but little attention has bee... more Rodent control tends to involve methods that cause animal suffering, but little attention has been paid to the animal welfare implications of rodent control. The aim of the current study was to gain insight into the opinions and attitudes of clients of Dutch pest controllers, regarding liminal rodents, rodent control, and rodent welfare. A better understanding of their attitudes may contribute to more ethical rodent management programmes. An online survey among 248 clients of Dutch pest controllers was carried out. Respondents, especially those within the agricultural sector, have a relatively negative attitude towards rats and mice. Respondents in the agricultural subgroup do not consider the welfare of liminal rodents important. They also think that the welfare impact of commonly used control methods is limited, and they have low tolerance levels for the presence of rodents. Respondents from other sectors have a far more positive attitude towards rats and mice, consider their welf...
To examine the dog ownership factors characteristic to a population of dogs confiscated after a h... more To examine the dog ownership factors characteristic to a population of dogs confiscated after a human and/ or animal-directed biting incident, we compared bite risk assessment reports of 159 confiscated dogs in the time frame 2008, 2009, 2010 (tf1) and of 215 confiscated dogs in the time frame 2020, 2021, 2022 (until mid-May; tf2). The reports were compiled by the same institute in a standardized format. We studied frequencies and chi-square pairwise comparisons (P<0.05) for 30 identified ownership factors. Overall (tf1 and tf2), 1,308 ownership factors were mentioned in the reports and reports mentioning ≥5 factors were twice as frequent in tf2 (38%) than tf1 (16%). Our data suggest that nine factors may in particular serve as a warning signal for biting incidents, as these factors were most frequently (≥15%) prevalent in the total of reported cases: having a multiple dog household, a dog reportedly roaming a neighbourhood without an owner, a dog’s care tasks being transferred, ...
Shelter dogs are exposed to a variety of stressors. Among non-invasive techniques, hair cortisol ... more Shelter dogs are exposed to a variety of stressors. Among non-invasive techniques, hair cortisol concentration (HCC) is suggested an easy to collect biomarker for giving insight into long-term stress responses. We evaluated HCC as an indicator of long-term cortisol responses in dogs in an animal shelter over different chronological time points during sheltering and after adoption. Hair samples were collected from the neck region following a shave/re-shave protocol of shelter dogs (total n = 52) at four different time periods: T1 intake at shelter (pre-shelter period, n = 51); T2 after 6 weeks in the shelter (n = 23); T3 6 weeks after adoption (n = 24); T4 6 months after adoption (n = 22). HCC at T2 was significantly higher than HCC at T1, T3 and T4 (effect of sample collection moment: F3,41 = 12.78, p < 0.0001). The dog’s weight class, age class, sex, reason for admission, kennel history and melanin type also explained HCC variability. No significant difference in HCC was found b...
Abstract Shelter dogs face the challenge of adapting to a kennel environment. Individual differen... more Abstract Shelter dogs face the challenge of adapting to a kennel environment. Individual differences in adaptation are known to exist. Resting patterns might be indicative of adaptability to such a novel environment, but need to be evaluated for its usefulness, like every potentially physiological and behavioural parameter. Here, we evaluated nocturnal activity patterns of dogs as indicators of adaptability to novel environments. We measured nocturnal activity (3-axial accelerometer, the Actical®) and two physiological stress parameters, i.e. urinary cortisol/creatinine ratio (UCCR) and body weight in 29 dogs relinquished to a shelter (SD group) in the first two days after intake at the shelter (n = 29), after a 12-day habituation period in the shelter (n = 28) and >6 weeks post-adoption (n = 17). A control group of 29 pet dogs kept at home (CPD group), matching the SD group characteristics, was also assessed for its nocturnal activity and UCCR. Linear mixed model analysis, t-tests and Friedman tests were used to analyse the data. The main findings are: 1) the SD group exhibited higher nocturnal activity (total activity counts, activity duration and number of rest bouts) the first two nights after intake than on night 12, with decreasing inter-individual variances. Compared to the CPD group they showed higher nocturnal activity on night 1 (all p In conclusion, nocturnal activity, as measured by an accelerometer, may be a valid parameter to monitor adaptability of dogs to a kennel environment. Monitoring nocturnal activity in this way can be a useful and cost-effective additional indicator for assessing dog welfare.
Journal of The American Association for Laboratory Animal Science, Sep 1, 2017
There is a strong need to gain systematic knowledge about the potential impact of routine procedu... more There is a strong need to gain systematic knowledge about the potential impact of routine procedures on laboratory animals to ensure animal welfare and reliability of experimental results. Blood sampling is a frequently used procedure within laboratory animal research. Two of the most common anesthesia-free techniques to obtain blood in mice are the saphenous venipuncture and submandibular bleeding. It is common practice that these methods are applied repeatedly to 1 individual. The impact of both techniques, either performed singly or repeatedly, on the animal’s welfare is not fully known yet. We directly compared the effects of both sampling methods on physiological stress responses, behavior, tissue damage, and procedure duration in 2 frequently used mouse strains: C57BL/6 and BALB/c mice (n = 45/strain). The mice were randomly assigned to 3 treatment groups (saphenous venipuncture, submandibular bleeding, control) of 15 mice/strain and were sampled weekly (80 μl/ blood sample) during 4 wk for the experimental groups and 5 wk for the control group. To evaluate the acute physiological stress response, plasma corticosterone levels were determined. As an indicator of chronic stress, body weights were recorded once a week and the thymus was weighed after necropsy. Behavior was recorded using video cameras in front of the cages and afterwards analyzed by a well-trained observer using event logging software. Tissue damage was evaluated by scoring pre-and postmortem hemorrhages and hematomas at the injection site and by measuring alanine transaminase, aspartate aminotransferase, and creatine kinase plasma levels. Submandibular bleeding appeared to be less time consuming (P<0.05) while providing higher sample volumes. However, this method led to increased acute stress responses in C57BL/6 mice (P = 0.03). All animals of both strains undergoing this procedure expressed more pain-related behavior (P < 0.05) and the extent of tissue damage at the injection site was significantly higher when compared to the saphenous venipuncture (P < 0.05). Thus, in order to avoid the undesirable effects of stress on experimental outcomes and to reduce the extent of tissue damage, saphenous venipuncture should be preferred above submandibular bleeding whenever possible.
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