Papers by Lieselot Hemeryck
Frontiers for Young Minds
Metabolites are substances that are formed within our bodies that help us live and grow. Examples... more Metabolites are substances that are formed within our bodies that help us live and grow. Examples include glucose and vitamin D. When changes happen in our bodies, like when we get sick, these metabolites can change. By studying metabolite changes, using a method called metabolomics, we can learn a lot about diseases, what causes them, and how to avoid them. We know that eating foods like fish, fruits, and vegetables is healthy. Eating too much red and processed meat, however, increases our chances of developing certain types of cancer. Unfortunately, we still do not understand why that is. It could be that there are unhealthy, toxic elements or substances in the meat itself or that there are toxic metabolites formed during or after the digestion of red and processed meat. To find out, we must journey into the gut, using metabolomics!
Bookmarks Related papers MentionsView impact
Frontiers in Veterinary Science, 2022
Obesity is a common problem in dogs and overconsumption of energy-rich foods is a key factor. Thi... more Obesity is a common problem in dogs and overconsumption of energy-rich foods is a key factor. This study compared the inflammatory response and fecal metabolome of dogs fed a high-fat vs. a high-starch diet. Ten healthy lean adult beagles were equally allocated into two groups in a cross-over design. Each group received two diets in which fat (horse fat) and starch (pregelatinized corn starch) were exchanged in an isocaloric way to compare high fat vs. high starch. There was a tendency to increase the glucose and glycine concentrations and the glucose/insulin ratio in the blood in dogs fed with the high-fat diet, whereas there was a decrease in the level of Non-esterified fatty acids and a tendency to decrease the alanine level in dogs fed with the high-starch diet. Untargeted analysis of the fecal metabolome revealed 10 annotated metabolites of interest, including L-methionine, which showed a higher abundance in dogs fed the high-starch diet. Five other metabolites were upregulated...
Bookmarks Related papers MentionsView impact
In 2015, the International Agency for Research on Cancer and the World Health Organization issued... more In 2015, the International Agency for Research on Cancer and the World Health Organization issued that red meat is ‘probably carcinogenic to humans’ and processed meat is ‘carcinogenic to humans’. Epidemiological research has demonstrated that red meat consumption significantly contributes to colorectal cancer (CRC) risk. Different hypotheses have been put forward to explain this causal relationship but the heme hypothesis, stating that heme iron in red meat stimulates the formation of genotoxic N-nitroso compounds (NOCs) and lipid peroxidation products (LPOs), has received the most support. Both NOCs and LPOs can exert DNA damaging effects like e.g. DNA adduct formation. DNA adduct formation is believed to be the first step in chemically induced carcinogenesis.
Bookmarks Related papers MentionsView impact
Scientific reports, Feb 14, 2017
Epidemiological research has demonstrated that the consumption of red meat is an important risk f... more Epidemiological research has demonstrated that the consumption of red meat is an important risk factor for the development of colorectal cancer (CRC), diabetes mellitus and cardiovascular diseases. However, there is no holistic insight in the (by-) products of meat digestion that may contribute to disease development. To address this hiatus, an untargeted mass spectrometry (MS)-based metabolomics approach was used to create red versus white meat associated metabolic fingerprints following in vitro colonic digestion using the fecal inocula of ten healthy volunteers. Twenty-two metabolites were unequivocally associated with simulated colonic digestion of red meat. Several of these metabolites could mechanistically be linked to red meat-associated pathways including N'-formylkynurenine, kynurenine and kynurenic acid (all involved in tryptophan metabolism), the oxidative stress marker dityrosine, and 3-dehydroxycarnitine. In conclusion, the used MS-based metabolomics platform proved...
Bookmarks Related papers MentionsView impact
Analytical Chemistry, 2016
Bookmarks Related papers MentionsView impact
Nutrition Reviews, 2016
Bookmarks Related papers MentionsView impact
Toxicology Research, 2016
Red meat digestion may contribute to colorectal cancer risk.
Bookmarks Related papers MentionsView impact
Food Chemistry, 2015
Bookmarks Related papers MentionsView impact
Journal of Agricultural and Food Chemistry, 2012
Bookmarks Related papers MentionsView impact
Scientific Reports, 2021
Hirschsprung's disease (HD) is a congenital structural abnormality of the colon seen in appro... more Hirschsprung's disease (HD) is a congenital structural abnormality of the colon seen in approximately 1 to 5000 live births. Despite surgical correction shortly after presentation, up to 60% of patients will express long-term gastrointestinal complaints, including potentially life-threatening Hirschsprung-associated enterocolitis (HAEC). In this study fecal samples from postoperative HD patients (n = 38) and their healthy siblings (n = 21) were analysed using high-resolution liquid chromatography—mass spectrometry aiming to further unravel the nature of the chronic gastrointestinal disturbances. Furthermore, within the patient group, we compared the faecal metabolome between patients with and without a history of HAEC as well as those diagnosed with short or long aganglionic segment. Targeted analysis identified several individual metabolites characteristic for all HD patients as well as those with a history of HAEC and long segment HD. Moreover, multivariate models based on unt...
Bookmarks Related papers MentionsView impact
Food Chemistry
Bookmarks Related papers MentionsView impact
Environment International
Bookmarks Related papers MentionsView impact
Meat Science
Bookmarks Related papers MentionsView impact
Food Chemistry
Bookmarks Related papers MentionsView impact
Genes & Nutrition
Meat, including fish and shellfish, represents a valuable constituent of most balanced diets. Con... more Meat, including fish and shellfish, represents a valuable constituent of most balanced diets. Consumption of different types of meat and fish has been associated with both beneficial and adverse health effects. While white meats and fish are generally associated with positive health outcomes, red and especially processed meats have been associated with colorectal cancer and other diseases. The contribution of these foods to the development or prevention of chronic diseases is still not fully elucidated. One of the main problems is the difficulty in properly evaluating meat intake, as the existing self-reporting tools for dietary assessment may be imprecise and therefore affected by systematic and random errors. Dietary biomarkers measured in biological fluids have been proposed as possible objective measurements of the actual intake of specific foods and as a support for classical assessment methods. Good biomarkers for meat intake should reflect total dietary intake of meat, indepe...
Bookmarks Related papers MentionsView impact
Molecular Nutrition & Food Research
Bookmarks Related papers MentionsView impact
Analytica Chimica Acta
Bookmarks Related papers MentionsView impact
Molecular nutrition & food research, Jan 3, 2018
The life sciences are currently being transformed by an unprecedented wave of developments in mol... more The life sciences are currently being transformed by an unprecedented wave of developments in molecular analysis, which include important advances in instrumental analysis as well as biocomputing. In light of the central role played by metabolism in nutrition, metabolomics is rapidly being established as a key analytical tool in human nutritional studies. Consequently, an increasing number of nutritionists integrate metabolomics into their study designs. Within this dynamic landscape, the potential of nutritional metabolomics (nutrimetabolomics) to be translated into a science, which can impact on health policies, still needs to be realized. A key element to reach this goal is the ability of the research community to join, to collectively make the best use of the potential offered by nutritional metabolomics. This article, therefore, provides a methodological description of nutritional metabolomics that reflects on the state-of-the-art techniques used in the laboratories of the Food...
Bookmarks Related papers MentionsView impact
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 2018
The consumption of red meat has been linked to an increased colorectal cancer (CRC) risk. One of ... more The consumption of red meat has been linked to an increased colorectal cancer (CRC) risk. One of the major hypotheses states that heme iron (present in red meat) stimulates the formation of genotoxic N-nitroso compounds (NOCs) and lipid peroxidation products (LPOs). By means of DNA adductomics, chemically induced DNA adduct formation can be mapped in relation to e.g. dietary exposures. In this study, this state-of-the-art methodology was used to investigate alkylation and (lipid per)oxidation induced DNA adduct formation in in vitro red vs. white meat digests. In doing so, 90 alkylation and (lipid per)oxidation induced DNA adduct types could be (tentatively) identified. Overall, 12 NOC- and/or LPO-related DNA adduct types, i.e. dimethyl-T (or ethyl-T), hydroxymethyl-T, tetramethyl-T, methylguanine (MeG), guanidinohydantoin, hydroxybutyl-C, hydroxymethylhydantoin, malondialdehyde-x3-C, O-carboxymethylguanine, hydroxyethyl-T, carboxyethyl-T and 3,N-etheno-C were singled out as potenti...
Bookmarks Related papers MentionsView impact
Food Chemistry
Digestion of red and processed meat has been linked to the formation of genotoxic N-nitroso compo... more Digestion of red and processed meat has been linked to the formation of genotoxic N-nitroso compounds (NOCs) and lipid peroxidation products (LPOs) in the gut. In this study, rats were fed a meat based diet to compare the possible genotoxic effects of red vs. white meat, and the interfering role of dietary fat. To this purpose, liver, duodenum and colon DNA adductomes were analyzed with UHPLC-HRMS. The results demonstrate that the consumed meat type alters the DNA adductome; the levels of 22 different DNA adduct types significantly increased upon the consumption of beef (compared to chicken) and/or lard supplemented beef or chicken. Furthermore, the chemical constitution of the retrieved DNA adducts hint at a direct link with an increase in NOCs and LPOs upon red (and processed) meat digestion, supporting the current hypotheses on the causal link between red and processed meat consumption and the development of colorectal cancer.
Bookmarks Related papers MentionsView impact
Uploads
Papers by Lieselot Hemeryck