Resumen es: Los monos neotropicales de la coleccion del Zoologico de la Ciudad de Mendoza (ZMA), ... more Resumen es: Los monos neotropicales de la coleccion del Zoologico de la Ciudad de Mendoza (ZMA), Argentina, fueron objeto de un estudio colaborativo que incluyo la t...
Scope The journal/newsletter aims to provide a basis for conservation information relating to the... more Scope The journal/newsletter aims to provide a basis for conservation information relating to the primates of the Neotropics. We welcome texts on any aspect of primate conservation, including articles, thesis abstracts, news items, recent events, recent publications, primatological society information and suchlike. Submissions Please send all English and Spanish contributions to: These manuscripts are usually reviewed only by the editors. A broader range of topics is encouraged, including such as behavioral research, in the interests of informing on general research activities that contribute to our understanding of platyrrhines. We encourage reports on projects and conservation and research programs (who, what, where, when, why, etc.) and most particularly information on geographical distributions, locality records, and protected areas and the primates that occur in them. Text should be typewritten, double-spaced with no less than 12 cpi (preferably Times New Roman) font and 3-cm m...
Given their great variability, microsatellites or STRs became the most commonly used genetic mark... more Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.
Background Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, con... more Background Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail. Results Although in the vast majority of pachytene cells all autosomes were paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21. Conclusion Our results suggest that C blocks...
Prometaphase Chromosomes of the Howler Monkey (Aloutta caraya): G, C, NOR, and Restriction Enzyme... more Prometaphase Chromosomes of the Howler Monkey (Aloutta caraya): G, C, NOR, and Restriction Enzyme (RE,) Banding ... M. MUDRY', M. PONSA', A. BORRELL3, J. EGOZCUE', AND M. GARCIA 'GIBE (Grupo de lnvestigacidn en Biologia Evolutiuai, Departamento de ...
The morphological and morphometric characterization of spermatozoa has been used as a taxonomic a... more The morphological and morphometric characterization of spermatozoa has been used as a taxonomic and phylogenetic tool for different species of mammals. We evaluated and compared the sperm morphometry of five neotropical primate species: Alouatta caraya, Ateles belzebuth and Ateles chamek of family Atelidae; and Cebus cay (=Sapajus cay) and Cebus nigritus (=Sapajus nigritus) of family Cebidae. After the collection of semen samples, the following parameters were measured on 100 spermatozoa from each specimen: Head Length, Head Width, Acrosome Length, Midpiece Length, Midpiece Width and Tail Length. Considering the available literature on sperm morphometry, we gathered data of 75 individuals, from 20 species, 8 genera and 2 families. These data were superimposed on a phylogeny to infer the possible direction of evolutionary changes. Narrower and shorter spermatozoa seem to be the ancestral form for Cebidae, with a trend toward wider and larger heads in derived groups. The spermatozoa o...
An assessment of the status of specimens of New World primates (Infraorder Platyrrhini) was made ... more An assessment of the status of specimens of New World primates (Infraorder Platyrrhini) was made at four Argentinean museums, with an emphasis at the “Museo Argentino de Ciencias Naturales” (MACN) and the “Museo de La Plata” (MLP). This involved examination and evaluation of skins and skulls to determine general condition and preservation needs. The goals were to: a) collect information on species represented, numbers of individuals, and geographic origin of specimens, and b) assess the condition of specimens and value of collection for multiple research uses, and for educational purposes in general. We present several recommendations to ensure the preservation and optimal use of Argentinean collections in the future. This study is presented as a model for more extensive surveys of museum specimens in Argentina and other parts of the world.
Ecotoxicology and environmental safety, Jan 31, 2018
Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were te... more Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard...
In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural are... more In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 μmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the o...
Resumen es: Los monos neotropicales de la coleccion del Zoologico de la Ciudad de Mendoza (ZMA), ... more Resumen es: Los monos neotropicales de la coleccion del Zoologico de la Ciudad de Mendoza (ZMA), Argentina, fueron objeto de un estudio colaborativo que incluyo la t...
Scope The journal/newsletter aims to provide a basis for conservation information relating to the... more Scope The journal/newsletter aims to provide a basis for conservation information relating to the primates of the Neotropics. We welcome texts on any aspect of primate conservation, including articles, thesis abstracts, news items, recent events, recent publications, primatological society information and suchlike. Submissions Please send all English and Spanish contributions to: These manuscripts are usually reviewed only by the editors. A broader range of topics is encouraged, including such as behavioral research, in the interests of informing on general research activities that contribute to our understanding of platyrrhines. We encourage reports on projects and conservation and research programs (who, what, where, when, why, etc.) and most particularly information on geographical distributions, locality records, and protected areas and the primates that occur in them. Text should be typewritten, double-spaced with no less than 12 cpi (preferably Times New Roman) font and 3-cm m...
Given their great variability, microsatellites or STRs became the most commonly used genetic mark... more Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.
Background Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, con... more Background Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail. Results Although in the vast majority of pachytene cells all autosomes were paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21. Conclusion Our results suggest that C blocks...
Prometaphase Chromosomes of the Howler Monkey (Aloutta caraya): G, C, NOR, and Restriction Enzyme... more Prometaphase Chromosomes of the Howler Monkey (Aloutta caraya): G, C, NOR, and Restriction Enzyme (RE,) Banding ... M. MUDRY&#x27;, M. PONSA&#x27;, A. BORRELL3, J. EGOZCUE&#x27;, AND M. GARCIA &#x27;GIBE (Grupo de lnvestigacidn en Biologia Evolutiuai, Departamento de ...
The morphological and morphometric characterization of spermatozoa has been used as a taxonomic a... more The morphological and morphometric characterization of spermatozoa has been used as a taxonomic and phylogenetic tool for different species of mammals. We evaluated and compared the sperm morphometry of five neotropical primate species: Alouatta caraya, Ateles belzebuth and Ateles chamek of family Atelidae; and Cebus cay (=Sapajus cay) and Cebus nigritus (=Sapajus nigritus) of family Cebidae. After the collection of semen samples, the following parameters were measured on 100 spermatozoa from each specimen: Head Length, Head Width, Acrosome Length, Midpiece Length, Midpiece Width and Tail Length. Considering the available literature on sperm morphometry, we gathered data of 75 individuals, from 20 species, 8 genera and 2 families. These data were superimposed on a phylogeny to infer the possible direction of evolutionary changes. Narrower and shorter spermatozoa seem to be the ancestral form for Cebidae, with a trend toward wider and larger heads in derived groups. The spermatozoa o...
An assessment of the status of specimens of New World primates (Infraorder Platyrrhini) was made ... more An assessment of the status of specimens of New World primates (Infraorder Platyrrhini) was made at four Argentinean museums, with an emphasis at the “Museo Argentino de Ciencias Naturales” (MACN) and the “Museo de La Plata” (MLP). This involved examination and evaluation of skins and skulls to determine general condition and preservation needs. The goals were to: a) collect information on species represented, numbers of individuals, and geographic origin of specimens, and b) assess the condition of specimens and value of collection for multiple research uses, and for educational purposes in general. We present several recommendations to ensure the preservation and optimal use of Argentinean collections in the future. This study is presented as a model for more extensive surveys of museum specimens in Argentina and other parts of the world.
Ecotoxicology and environmental safety, Jan 31, 2018
Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were te... more Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard...
In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural are... more In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 μmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the o...
Uploads
Papers