Abstract Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized b... more Abstract Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3–5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%.
Fil: Faletti, Laura Eva. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de C... more Fil: Faletti, Laura Eva. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Nanobiotecnologia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Nanobiotecnologia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Microbiologia, Inmunologia y Biotecnologia. Catedra de Microbiologia Industrial y Biotecnologia; Argentina
This chapter summarizes and brings a critical evaluation of the advantages and disadvantages of t... more This chapter summarizes and brings a critical evaluation of the advantages and disadvantages of the properties and applications of chitin for sorption processes. Also discusses potential physical and chemical modifications and future considerations. The study of the adsorptive properties and the ways for their enhancement or increasing the selectivity for a specific sorbate is a growing topic as a consequence of the need for more controlled pollutant effluent management. Moreover, the possibilities of isolating valuable or marketable molecules from those effluents or industrial subproducts demand the development of novel sorbents that satisfy the regulatory conditions and the process economy. Chitin is the second most abundant natural polymer and may, at least potentially, fulfill these requirements.
International Journal of Biological Macromolecules, 2021
In this work, the influence of Sodium Acetate Trihydrate (SAT) on the gelling stage of a chitin h... more In this work, the influence of Sodium Acetate Trihydrate (SAT) on the gelling stage of a chitin hydrogel was studied. Characterization techniques, such as FTIR, Raman, solid-state NMR, Dielectric Spectroscopy, Small-angle X-ray scattering (SAXS), Wide-angle X-ray scattering (WAXS), and X-ray diffraction (XRD) were used to study the effect of SAT on the micro and nanostructure of the material in the wet, dry and freeze-dried states. It was demonstrated that the amount of SAT in the gelling solution can induce a variation in the supramolecular interaction among the polysaccharide chains, which leads to a change in the structural characteristics. In addition, it was observed that the polymer-water interactions are also altered by this structural ordering. Also, the affinity interaction with lysozyme was evaluated and an influence on the adsorption capacity was evidenced with the use of SAT. This could be an advance for biotechnological, biomedical, and food applications.
Chitin: Properties, Applications and Research, 2017
This review describes the use of chitosan for high value proteinrecovery, especially from raw mat... more This review describes the use of chitosan for high value proteinrecovery, especially from raw materials. In the last decade, due to itschemical, mechanical and structural properties, chitosan has been gainingattention in numerous application fields including downstream processingof proteins. The search for low cost chromatographic supports presents adrawback when the target protein is in a raw particulated material. Chitosan can be formatted to perform purification processes. In thissense, this work deals with the development and utilization of low costchitosan as affinity chromatographic matrices to purify different proteinsfrom sweet whey, equine serum, wheat germ extract, and insect cells andlarvae extracts. Chitosan matrices were synthesized in the format ofhighly porous mini-spheres with diameters around 1.8 mm in order to beused in protein purification processes in batch mode. These mini-sphereswere directly used as an affinity matrix to purify wheat germ agglutinin(WGA), or were derivatized by attaching different specific ligands, suchas WGA or triazine dyes, to purify sialylated proteins and cheese wheyproteins.Fil: Baieli, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Urtasun, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Hirsch, Daniela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Bracco, Lautaro Fidel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentin
... Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentra... more ... Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentrated Plus TM to separate the pectinesterase-containing fraction. ... Flavourzyme TM was from Novo Industries, Denmark. The Bioconcentrated Plus TM was from Biocon, Ireland. ...
El suero de queso o lactosuero es un subproducto de la fabricación del queso cuya producción mund... more El suero de queso o lactosuero es un subproducto de la fabricación del queso cuya producción mundial anual ronda los 200 millones de toneladas. Su contenido proteico es de 0,6 %, lo que significa 1,2 millones de toneladas de proteínas anuales. Este subproducto industrial contiene proteínas de alto valor comercial todavía no recuperadas eficientemente en nuestro país y en muchos lugares del mundo, siendo su mayor valorización la producción de suero en polvo, lactosa o concentrados proteicos totales. En este artículo se muestra la gran cantidad de proteínas y otras moléculas que pueden ser recuperadas a partir del lactosuero y se describen los métodos utilizados para su purificación. Dentro de las proteínas de alto valor se encuentran la lactoferrina, lactoperoxidasa y la osteopontina, además de las de valor intermedio como la β-lactoglobulina, α-lactalbúmina, caseinomacropéptido e inmunoglobulinas. La purificación de estas proteínas haría rentable la manufactura de un desecho industr...
Fil: Urtasun, Nicolas. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coo... more Fil: Urtasun, Nicolas. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Nanobiotecnologia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Nanobiotecnologia; Argentina
Poly(ethylene) hollow-fibre membranes with immobilised Cibacron Blue F3G-A were obtained in four ... more Poly(ethylene) hollow-fibre membranes with immobilised Cibacron Blue F3G-A were obtained in four different ways from epoxy-activated fibres. Membranes with a maximum capacity of 26 mg lysozyme ml-1and a dye density of 52 µmol ml-1were obtained when ammonia was used to open the epoxy group before dye immobilisation. Pure water flux of the modified membranes at 1 bar pressure was 1.0 cm min-1, thus meaning only a
Abstract Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized b... more Abstract Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3–5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%.
Fil: Faletti, Laura Eva. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de C... more Fil: Faletti, Laura Eva. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Nanobiotecnologia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Nanobiotecnologia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Microbiologia, Inmunologia y Biotecnologia. Catedra de Microbiologia Industrial y Biotecnologia; Argentina
This chapter summarizes and brings a critical evaluation of the advantages and disadvantages of t... more This chapter summarizes and brings a critical evaluation of the advantages and disadvantages of the properties and applications of chitin for sorption processes. Also discusses potential physical and chemical modifications and future considerations. The study of the adsorptive properties and the ways for their enhancement or increasing the selectivity for a specific sorbate is a growing topic as a consequence of the need for more controlled pollutant effluent management. Moreover, the possibilities of isolating valuable or marketable molecules from those effluents or industrial subproducts demand the development of novel sorbents that satisfy the regulatory conditions and the process economy. Chitin is the second most abundant natural polymer and may, at least potentially, fulfill these requirements.
International Journal of Biological Macromolecules, 2021
In this work, the influence of Sodium Acetate Trihydrate (SAT) on the gelling stage of a chitin h... more In this work, the influence of Sodium Acetate Trihydrate (SAT) on the gelling stage of a chitin hydrogel was studied. Characterization techniques, such as FTIR, Raman, solid-state NMR, Dielectric Spectroscopy, Small-angle X-ray scattering (SAXS), Wide-angle X-ray scattering (WAXS), and X-ray diffraction (XRD) were used to study the effect of SAT on the micro and nanostructure of the material in the wet, dry and freeze-dried states. It was demonstrated that the amount of SAT in the gelling solution can induce a variation in the supramolecular interaction among the polysaccharide chains, which leads to a change in the structural characteristics. In addition, it was observed that the polymer-water interactions are also altered by this structural ordering. Also, the affinity interaction with lysozyme was evaluated and an influence on the adsorption capacity was evidenced with the use of SAT. This could be an advance for biotechnological, biomedical, and food applications.
Chitin: Properties, Applications and Research, 2017
This review describes the use of chitosan for high value proteinrecovery, especially from raw mat... more This review describes the use of chitosan for high value proteinrecovery, especially from raw materials. In the last decade, due to itschemical, mechanical and structural properties, chitosan has been gainingattention in numerous application fields including downstream processingof proteins. The search for low cost chromatographic supports presents adrawback when the target protein is in a raw particulated material. Chitosan can be formatted to perform purification processes. In thissense, this work deals with the development and utilization of low costchitosan as affinity chromatographic matrices to purify different proteinsfrom sweet whey, equine serum, wheat germ extract, and insect cells andlarvae extracts. Chitosan matrices were synthesized in the format ofhighly porous mini-spheres with diameters around 1.8 mm in order to beused in protein purification processes in batch mode. These mini-sphereswere directly used as an affinity matrix to purify wheat germ agglutinin(WGA), or were derivatized by attaching different specific ligands, suchas WGA or triazine dyes, to purify sialylated proteins and cheese wheyproteins.Fil: Baieli, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Urtasun, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Hirsch, Daniela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Bracco, Lautaro Fidel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentin
... Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentra... more ... Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentrated Plus TM to separate the pectinesterase-containing fraction. ... Flavourzyme TM was from Novo Industries, Denmark. The Bioconcentrated Plus TM was from Biocon, Ireland. ...
El suero de queso o lactosuero es un subproducto de la fabricación del queso cuya producción mund... more El suero de queso o lactosuero es un subproducto de la fabricación del queso cuya producción mundial anual ronda los 200 millones de toneladas. Su contenido proteico es de 0,6 %, lo que significa 1,2 millones de toneladas de proteínas anuales. Este subproducto industrial contiene proteínas de alto valor comercial todavía no recuperadas eficientemente en nuestro país y en muchos lugares del mundo, siendo su mayor valorización la producción de suero en polvo, lactosa o concentrados proteicos totales. En este artículo se muestra la gran cantidad de proteínas y otras moléculas que pueden ser recuperadas a partir del lactosuero y se describen los métodos utilizados para su purificación. Dentro de las proteínas de alto valor se encuentran la lactoferrina, lactoperoxidasa y la osteopontina, además de las de valor intermedio como la β-lactoglobulina, α-lactalbúmina, caseinomacropéptido e inmunoglobulinas. La purificación de estas proteínas haría rentable la manufactura de un desecho industr...
Fil: Urtasun, Nicolas. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coo... more Fil: Urtasun, Nicolas. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Nanobiotecnologia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Nanobiotecnologia; Argentina
Poly(ethylene) hollow-fibre membranes with immobilised Cibacron Blue F3G-A were obtained in four ... more Poly(ethylene) hollow-fibre membranes with immobilised Cibacron Blue F3G-A were obtained in four different ways from epoxy-activated fibres. Membranes with a maximum capacity of 26 mg lysozyme ml-1and a dye density of 52 µmol ml-1were obtained when ammonia was used to open the epoxy group before dye immobilisation. Pure water flux of the modified membranes at 1 bar pressure was 1.0 cm min-1, thus meaning only a
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