Transdifferentiation of keratocytes into fibroblasts or further into myofibroblasts, which produc... more Transdifferentiation of keratocytes into fibroblasts or further into myofibroblasts, which produced denser and more disorganized extracellular matrix, is the major cause of corneal fibrosis and scarring, leading to corneal blindness. TGF-β1 is the critical cytokine for the myofibroblast’s transdifferentiation and survival. Hypoxia Inducible Factor (HIF) was found to play an important role in promoting fibrosis in lung, kidney, and dermal tissues recently. Our preliminary study demonstrated that topical administration of the acriflavine (ACF), a drug inhibiting HIF dimerization, delayed corneal opacity and neovascularization after the alkali burn. To know whether ACF could prevent corneal fibrosis and improve corneal transparency, we created a mouse mechanical corneal injury model and found that topical administration of ACF significantly inhibited corneal fibrosis at day 14 post-injury. The reduction of myofibroblast marker α-SMA, and fibronectin, one of the disorganized extracellul...
We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA m... more We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA methylation (epitranscriptomic) regulators (m6A-RMRs) in 41 diseases and cancers and made significant findings: (1) a few m6A-RMRs were upregulated; and most m6A-RMRs were downregulated in sepsis, acute respiratory distress syndrome, shock, and trauma; (2) half of 29 m6A-RMRs were downregulated in atherosclerosis; (3) inflammatory bowel disease and rheumatoid arthritis modulated m6A-RMRs more than lupus and psoriasis; (4) some organ failures shared eight upregulated m6A-RMRs; end-stage renal failure (ESRF) downregulated 85% of m6A-RMRs; (5) Middle-East respiratory syndrome coronavirus infections modulated m6A-RMRs the most among viral infections; (6) proinflammatory oxPAPC modulated m6A-RMRs more than DAMP stimulation including LPS and oxLDL; (7) upregulated m6A-RMRs were more than downregulated m6A-RMRs in cancer types; five types of cancers upregulated ≥10 m6A-RMRs; (8) proinflammatory ...
Arteriosclerosis, Thrombosis, and Vascular Biology, 2017
Metabolically healthy obesity (MHO) describes the phenomenon of overweight and obese patients par... more Metabolically healthy obesity (MHO) describes the phenomenon of overweight and obese patients paradoxically retaining a healthy metabolic profile. The molecular mechanisms underlying MHO remain enigmatic partly due to a dearth of animal models mirroring MHO in patients. Using apolipoprotein E knockout (ApoE -/- ) mice on high-fat (HF) diet as an atherosclerotic obesity model, we demonstrated: 1) microRNA-155 (miRNA-155, miR-155) is significantly upregulated in aortas of ApoE -/- mice; and miR-155 deficiency in ApoE -/- mice inhibits atherosclerosis; 2) ApoE -/- /miR-155 -/- (DKO) mice show HF diet-induced obesity, adipocyte hypertrophy and present with nonalcoholic fatty liver disease (NAFLD); 3) DKO mice demonstrate HF diet-induced elevations of plasma leptin, resistin, fed-state and fasting insulin, increased expression of adipogenic transcription factors, but lack glucose intolerance and insulin resistance. Our results are the first to present a metabolically healthy obesity (MHO...
Forty-eight nuclear receptors are associated with SAH and SAM/SAH ratio in mouse tissues. A: show... more Forty-eight nuclear receptors are associated with SAH and SAM/SAH ratio in mouse tissues. A: shows the correlation of 48 nuclear receptors associated with SAH levels (hypomethylation status) in mouse tissues. B. shows the correlation of 48 nuclear receptors associated with SAM/SAH ratio in mouse tissues. (PDF 648Â kb)
Nuclear receptors are differently expressed in human and mouse tissues. A: representative tissue ... more Nuclear receptors are differently expressed in human and mouse tissues. A: representative tissue mRNA distribution profile of housekeeping gene ARHGDIA in humans and Ldha in mice. See â Experimental Proceduresâ for details. B: mRNA distribution profiles of 26 nuclear receptors in 21 human tissues. C: MRNA distribution profiles of 15 nuclear receptors in 17 mouse tissues. The statistical significance was defined as when gene expression was larger than the upper limit of the confidence interval. Gene symbols are listed in Tables 1, 2, and 3. (PDF 296Â kb)
In order to solve the problems of time consuming, energy consumption and low simulation accuracy ... more In order to solve the problems of time consuming, energy consumption and low simulation accuracy in the hot air-drying system of food drying. Using computational fluid dynamics (CFD) to simulate the drying process of mushrooms can provide a reference for its technology research and development (R&D). The porous media approach was used to model the flow resistance offered by the mushroom. The resistance coefficient and porosity were determined through the experiments. Different air supply ways (wind speed, temperature, fresh air volume, reverse air supply period) were simulated and two optimize ways were suggested according the shortest possible drying time. The simulation results are in good agreement with the experimental results. The air supply way of periodic with reverse (SMPR), which means timed alternate the direction of air flow, or the way of mix in fresh air intermittently can effectively shorten the drying time. Considering comprehensively, the optimal air supply way was t...
Objective To investigate the application effects of traditional Chinese medicine (TCM) nursing in... more Objective To investigate the application effects of traditional Chinese medicine (TCM) nursing intervention in patients with ovarian hyper-stimulation syndrome. Methods A total of 60 cases with ovarian hyper-stimulation syndrome were divided into observation group (n=30) and control group (n=30). The patients of control group received normal nursing care, and the patients of observation group received TCM nursing intervention based on the routine care. The TCM nursing interventions included TCM mental nursing, diet nursing, and TCM nursing skills such as acupoint massage and auricular plaster therapy. The scores of anxiety assessment (SAS) after a week of the admission, the quality of life (QOL-BREF) and the nursing satisfaction when discharging from the hospital were compared between the two groups. Results After a week of the admission, the scores of SAS, QOL-BREF, and nursing satisfaction for the observation group were significantly better than those of the control group (P<0....
Background Astrocytes play an essential role in the modulation of blood-brain barrier function. N... more Background Astrocytes play an essential role in the modulation of blood-brain barrier function. Neurological diseases induce astrocytes to transform into a neurotoxic A1 phenotype, thus exacerbating brain injury. However, the effect of A1 astrocyte on the function of BBB after stroke is unknown. Method: Adult male ICR mice (n = 78) were subjected to 90-minute transient middle cerebral artery occlusion. Immunohistochemical staining of A1 (C3d) and A2 (S100A10) was performed to characterize phenotypic changes of astrocytes overtime after stroke. Glucagon-like peptide-1 receptor agonist semaglutide was intraperitoneally injected into the mice to inhibit A1 astrocyte. Infarct volume, atrophy volume, neurobehavioral outcomes, and BBB permeability were examined. RNA-seq was adopted to explore the potential targets and signaling pathways of A1 astrocytes induced BBB dysfunction. Results Astrocytes assumed the A2 phenotype at the early stage of ischemic stroke but gradually transformed to t...
Metabolically healthy obesity (MHO) accounts for roughly 35% of all obese patients. There is no c... more Metabolically healthy obesity (MHO) accounts for roughly 35% of all obese patients. There is no clear consensus that has been reached on whether MHO is a stable condition or merely a transitory period between metabolically healthy lean and metabolically unhealthy obesity (MUO). Additionally, the mechanisms underlying MHO and any transition to MUO are not clear. Macrophages are the most common immune cells in adipose tissues and have a significant presence in atherosclerosis. Fas (or CD95), which is highly expressed on macrophages, is classically recognized as a pro-apoptotic cell surface receptor. However, Fas also plays a significant role as a pro-inflammatory molecule. Previously, we established a mouse model (ApoE-/-/miR155-/-; DKO mouse) of MHO, based on the criteria of not having metabolic syndrome (MetS) and insulin resistance (IR). In our current study, we hypothesized that MHO is a transition phase toward MUO, and that inflammation driven by our newly classified CD95+CD86- m...
Arteriosclerosis, Thrombosis, and Vascular Biology, 2019
Objective: Hyperhomocysteinemia (HHcy) is a potent risk factor for diabetic cardiovascular diseas... more Objective: Hyperhomocysteinemia (HHcy) is a potent risk factor for diabetic cardiovascular diseases. We have previously reported that hyperhomocysteinemia potentiates type 1 diabetes mellitus-induced inflammatory monocyte differentiation, vascular dysfunction, and atherosclerosis. However, the effects of hyperhomocysteinemia on vascular inflammation in type 2 diabetes mellitus (T2DM) and the underlying mechanism are unknown. Approach and Results: Here, we demonstrate that hyperhomocysteinemia was induced by a high methionine diet in control mice (homocysteine 129 µmol/L), which was further worsened in T2DM db/db mice (homocysteine 180 µmol/L) with aggravated insulin intolerance. Hyperhomocysteinemia potentiated T2DM-induced mononuclear cell, monocyte, inflammatory monocyte (CD11b + Ly6C + ), and M1 macrophage differentiation in periphery and aorta, which were rescued by folic acid-based homocysteine-lowering therapy. Moreover, hyperhomocysteinemia exacerbated T2DM-impaired endotheli...
Transdifferentiation of keratocytes into fibroblasts or further into myofibroblasts, which produc... more Transdifferentiation of keratocytes into fibroblasts or further into myofibroblasts, which produced denser and more disorganized extracellular matrix, is the major cause of corneal fibrosis and scarring, leading to corneal blindness. TGF-β1 is the critical cytokine for the myofibroblast’s transdifferentiation and survival. Hypoxia Inducible Factor (HIF) was found to play an important role in promoting fibrosis in lung, kidney, and dermal tissues recently. Our preliminary study demonstrated that topical administration of the acriflavine (ACF), a drug inhibiting HIF dimerization, delayed corneal opacity and neovascularization after the alkali burn. To know whether ACF could prevent corneal fibrosis and improve corneal transparency, we created a mouse mechanical corneal injury model and found that topical administration of ACF significantly inhibited corneal fibrosis at day 14 post-injury. The reduction of myofibroblast marker α-SMA, and fibronectin, one of the disorganized extracellul...
We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA m... more We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA methylation (epitranscriptomic) regulators (m6A-RMRs) in 41 diseases and cancers and made significant findings: (1) a few m6A-RMRs were upregulated; and most m6A-RMRs were downregulated in sepsis, acute respiratory distress syndrome, shock, and trauma; (2) half of 29 m6A-RMRs were downregulated in atherosclerosis; (3) inflammatory bowel disease and rheumatoid arthritis modulated m6A-RMRs more than lupus and psoriasis; (4) some organ failures shared eight upregulated m6A-RMRs; end-stage renal failure (ESRF) downregulated 85% of m6A-RMRs; (5) Middle-East respiratory syndrome coronavirus infections modulated m6A-RMRs the most among viral infections; (6) proinflammatory oxPAPC modulated m6A-RMRs more than DAMP stimulation including LPS and oxLDL; (7) upregulated m6A-RMRs were more than downregulated m6A-RMRs in cancer types; five types of cancers upregulated ≥10 m6A-RMRs; (8) proinflammatory ...
Arteriosclerosis, Thrombosis, and Vascular Biology, 2017
Metabolically healthy obesity (MHO) describes the phenomenon of overweight and obese patients par... more Metabolically healthy obesity (MHO) describes the phenomenon of overweight and obese patients paradoxically retaining a healthy metabolic profile. The molecular mechanisms underlying MHO remain enigmatic partly due to a dearth of animal models mirroring MHO in patients. Using apolipoprotein E knockout (ApoE -/- ) mice on high-fat (HF) diet as an atherosclerotic obesity model, we demonstrated: 1) microRNA-155 (miRNA-155, miR-155) is significantly upregulated in aortas of ApoE -/- mice; and miR-155 deficiency in ApoE -/- mice inhibits atherosclerosis; 2) ApoE -/- /miR-155 -/- (DKO) mice show HF diet-induced obesity, adipocyte hypertrophy and present with nonalcoholic fatty liver disease (NAFLD); 3) DKO mice demonstrate HF diet-induced elevations of plasma leptin, resistin, fed-state and fasting insulin, increased expression of adipogenic transcription factors, but lack glucose intolerance and insulin resistance. Our results are the first to present a metabolically healthy obesity (MHO...
Forty-eight nuclear receptors are associated with SAH and SAM/SAH ratio in mouse tissues. A: show... more Forty-eight nuclear receptors are associated with SAH and SAM/SAH ratio in mouse tissues. A: shows the correlation of 48 nuclear receptors associated with SAH levels (hypomethylation status) in mouse tissues. B. shows the correlation of 48 nuclear receptors associated with SAM/SAH ratio in mouse tissues. (PDF 648Â kb)
Nuclear receptors are differently expressed in human and mouse tissues. A: representative tissue ... more Nuclear receptors are differently expressed in human and mouse tissues. A: representative tissue mRNA distribution profile of housekeeping gene ARHGDIA in humans and Ldha in mice. See â Experimental Proceduresâ for details. B: mRNA distribution profiles of 26 nuclear receptors in 21 human tissues. C: MRNA distribution profiles of 15 nuclear receptors in 17 mouse tissues. The statistical significance was defined as when gene expression was larger than the upper limit of the confidence interval. Gene symbols are listed in Tables 1, 2, and 3. (PDF 296Â kb)
In order to solve the problems of time consuming, energy consumption and low simulation accuracy ... more In order to solve the problems of time consuming, energy consumption and low simulation accuracy in the hot air-drying system of food drying. Using computational fluid dynamics (CFD) to simulate the drying process of mushrooms can provide a reference for its technology research and development (R&D). The porous media approach was used to model the flow resistance offered by the mushroom. The resistance coefficient and porosity were determined through the experiments. Different air supply ways (wind speed, temperature, fresh air volume, reverse air supply period) were simulated and two optimize ways were suggested according the shortest possible drying time. The simulation results are in good agreement with the experimental results. The air supply way of periodic with reverse (SMPR), which means timed alternate the direction of air flow, or the way of mix in fresh air intermittently can effectively shorten the drying time. Considering comprehensively, the optimal air supply way was t...
Objective To investigate the application effects of traditional Chinese medicine (TCM) nursing in... more Objective To investigate the application effects of traditional Chinese medicine (TCM) nursing intervention in patients with ovarian hyper-stimulation syndrome. Methods A total of 60 cases with ovarian hyper-stimulation syndrome were divided into observation group (n=30) and control group (n=30). The patients of control group received normal nursing care, and the patients of observation group received TCM nursing intervention based on the routine care. The TCM nursing interventions included TCM mental nursing, diet nursing, and TCM nursing skills such as acupoint massage and auricular plaster therapy. The scores of anxiety assessment (SAS) after a week of the admission, the quality of life (QOL-BREF) and the nursing satisfaction when discharging from the hospital were compared between the two groups. Results After a week of the admission, the scores of SAS, QOL-BREF, and nursing satisfaction for the observation group were significantly better than those of the control group (P<0....
Background Astrocytes play an essential role in the modulation of blood-brain barrier function. N... more Background Astrocytes play an essential role in the modulation of blood-brain barrier function. Neurological diseases induce astrocytes to transform into a neurotoxic A1 phenotype, thus exacerbating brain injury. However, the effect of A1 astrocyte on the function of BBB after stroke is unknown. Method: Adult male ICR mice (n = 78) were subjected to 90-minute transient middle cerebral artery occlusion. Immunohistochemical staining of A1 (C3d) and A2 (S100A10) was performed to characterize phenotypic changes of astrocytes overtime after stroke. Glucagon-like peptide-1 receptor agonist semaglutide was intraperitoneally injected into the mice to inhibit A1 astrocyte. Infarct volume, atrophy volume, neurobehavioral outcomes, and BBB permeability were examined. RNA-seq was adopted to explore the potential targets and signaling pathways of A1 astrocytes induced BBB dysfunction. Results Astrocytes assumed the A2 phenotype at the early stage of ischemic stroke but gradually transformed to t...
Metabolically healthy obesity (MHO) accounts for roughly 35% of all obese patients. There is no c... more Metabolically healthy obesity (MHO) accounts for roughly 35% of all obese patients. There is no clear consensus that has been reached on whether MHO is a stable condition or merely a transitory period between metabolically healthy lean and metabolically unhealthy obesity (MUO). Additionally, the mechanisms underlying MHO and any transition to MUO are not clear. Macrophages are the most common immune cells in adipose tissues and have a significant presence in atherosclerosis. Fas (or CD95), which is highly expressed on macrophages, is classically recognized as a pro-apoptotic cell surface receptor. However, Fas also plays a significant role as a pro-inflammatory molecule. Previously, we established a mouse model (ApoE-/-/miR155-/-; DKO mouse) of MHO, based on the criteria of not having metabolic syndrome (MetS) and insulin resistance (IR). In our current study, we hypothesized that MHO is a transition phase toward MUO, and that inflammation driven by our newly classified CD95+CD86- m...
Arteriosclerosis, Thrombosis, and Vascular Biology, 2019
Objective: Hyperhomocysteinemia (HHcy) is a potent risk factor for diabetic cardiovascular diseas... more Objective: Hyperhomocysteinemia (HHcy) is a potent risk factor for diabetic cardiovascular diseases. We have previously reported that hyperhomocysteinemia potentiates type 1 diabetes mellitus-induced inflammatory monocyte differentiation, vascular dysfunction, and atherosclerosis. However, the effects of hyperhomocysteinemia on vascular inflammation in type 2 diabetes mellitus (T2DM) and the underlying mechanism are unknown. Approach and Results: Here, we demonstrate that hyperhomocysteinemia was induced by a high methionine diet in control mice (homocysteine 129 µmol/L), which was further worsened in T2DM db/db mice (homocysteine 180 µmol/L) with aggravated insulin intolerance. Hyperhomocysteinemia potentiated T2DM-induced mononuclear cell, monocyte, inflammatory monocyte (CD11b + Ly6C + ), and M1 macrophage differentiation in periphery and aorta, which were rescued by folic acid-based homocysteine-lowering therapy. Moreover, hyperhomocysteinemia exacerbated T2DM-impaired endotheli...
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