Herbal drugs are habitually considered to have less toxic and almost free of side effects of synthetic ones. The plant Holarrhena antidysenterica is traditionally used in various diseases. Hence the present investigation has designed to appraise antihyperglycemic, antioxidant principle and acute toxicity of the aqueous and methanolic extracts of bark of Holarrhena antidysenterica (AEHA and MEHA). Antihyperglycemic effect was evaluated in alloxan induced diabetic rats. The oral administration of plant aqueous and methanolic extracts at dose of 200 and 400 mg/kg body weight was given in alloxan induced (150 mg/kg body weight) diabetic rats in comparison with reference drugs Glibenclamide (4 mg/kg body weight) and Insulin (5 unit/kg). The antioxidant potential of aqueous and methanolic of bark was checked by qualitative method and quantitatively through DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging assay, Nitric oxide scavenging assay and Reducing power activity methods. We have also studied the acute toxic effect of the crude extract. The extracts was suppressed rise in blood glucose after 90 mint in the acute glucose tolerance test and considerable drop in elevated blood glucose level was observed in the alloxan induced diabetic (p<0.01) rat. In vitro antioxidant screening techniques the extracts showed significant antioxidative potency. In case of acute toxicity study no death was found due to oral ingestion of crude extract. The present investigation suggests that the Holarrhena antidysenterica has both antihyperglycemic and antioxidant potential but no acute toxic effect.