Dietary flavonoid and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolase
Lactase phlorizin hydrolase (LPH; EC 3.2. 1.62) is a membrane‐bound, family 1 β‐
glycosidase found on the brush border of the mammalian small intestine. LPH, purified from
sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone
glycosides. The catalytic efficiency (k cat/K m) for the hydrolysis of quercetin‐4′‐glucoside,
quercetin‐3‐glucoside, genistein‐7‐glucoside and daidzein‐7‐glucoside was 170, 137, 77
and 14 (mM− 1 s− 1) respectively. The majority of the activity occurred at the lactase and not …
glycosidase found on the brush border of the mammalian small intestine. LPH, purified from
sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone
glycosides. The catalytic efficiency (k cat/K m) for the hydrolysis of quercetin‐4′‐glucoside,
quercetin‐3‐glucoside, genistein‐7‐glucoside and daidzein‐7‐glucoside was 170, 137, 77
and 14 (mM− 1 s− 1) respectively. The majority of the activity occurred at the lactase and not …
Canada
AJ Day - 2000 - historicplacesdays.ca