Above-ground biomass (AGB) directly relates to the productivity of forests. Precisely, AGB mapping for regional forests based on very high resolution (VHR) imagery is widely needed for evaluation of productivity. However, the diversity of variables and algorithms and the difficulties inherent in high resolution optical imagery make it complex. In this paper, we explored the potentials of the state-of-art algorithm convolutional neural networks (CNNs), which are widely used for its high-level representation, but rarely applied for AGB estimation. Four experiments were carried out to compare the performance of CNNs and other state-of-art Machine Learning (ML) algorithms: (1) performance of CNN using bands, (2) performance of Random Forest (RF), support vector regression (SVR), artificial neural network (ANN) on bands, and vegetation indices (VIs). (3) Performance of RF, SVR, and ANN on gray-level co-occurrence matrices (GLCM), and exploratory spatial data analysis (ESDA), and (4) performance of RF, SVR, and ANN based on all combined data and ESDA+VIs. CNNs reached satisfactory results (with R² = 0.943) even with limited input variables (i.e., only bands). In comparison, RF and SVR with elaborately designed data obtained slightly better accuracy than CNN. For examples, RF based on GLCM textures reached an R² of 0.979 and RF based on all combined data reached a close R² of 0.974. However, the results of ANN were much worse (with the best R² of 0.885).