FtsZ1-1 and MinD plastid division-related genes were identified and cloned from Brassica oleracea... more FtsZ1-1 and MinD plastid division-related genes were identified and cloned from Brassica oleracea var. botrytis. Transgenic tobacco plants expressing BoFtsZ1-1 or BoMinD exhibited cells with either fewer but abnormally large chloroplasts or more but smaller chloroplasts relative to wild-type tobacco plants. An abnormal chloroplast phenotype in guard cells was found in BoMinD transgenic tobacco plants but not in BoFtsZ1-1 transgenic tobacco plants. Transgenic tobacco plants bearing the macro-chloroplast phenotype had 10 to 20-fold increased levels of total FtsZ1-1 or MinD, whilst the transgenic tobacco plants bearing the mini-chloroplast phenotype had lower increased FtsZ1-1 or absence of detectable MinD. We also described for the first time, plastid transformation of macro-chloroplast bearing tobacco shoots with a gene cassette allowing for expression of green fluorescent protein (GFP). Homoplasmic plastid transformants from normal chloroplast and macro-chloroplast tobacco plants expressing GFP were obtained. Both types of transformants accumulated GFP at ~6% of total soluble protein, thus indicating that cells containing macro-chloroplasts can regenerate shoots in tissue culture and can stably integrate and express a foreign gene to similar levels as plant cells containing a normal chloroplast size and number.
Generation of transgenic trees for reproductive incompetence is an important application of genet... more Generation of transgenic trees for reproductive incompetence is an important application of genetic engineering to commercial forestry. This involves isolation and characterisation of the regulatory regions of the genes expressed at the very early stages of male and female reproductive organ development. A gene family showing specific expression in Pinus radiata immature male and female cone-buds was isolated and characterised. A cell ablation strategy involving expression of toxin genes, under the control of the regulatory sequences of these genes, led to the ablation of the target cells. The major problem with this strategy was 'promoter leakage', low (background) levels of expression of some flower-specific promoters in vegetative tissues during flowering and non-flowering periods. To improve the cell ablation strategy, a cascade of chimeric genes was designed. During non-flowering, controlled expression of the anti-toxin gene from Bacillus amyloliquefaciens, Barstar, in non-flowering tissues arrested the accumulation of the toxic Barnase protein.
Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigat... more Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigatus-Synechocystis pellets after flocculation. (A) Increase in lipids in A. fumigatus/GLU-SD pellets compare to the additive yields of lipid/intracellular FFA and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. (B) Increase in lipids in A. fumigatus/TWS-SD pellets compare to the additive yields of lipid/intracellular FFAs and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. Significance levels: *) p<0.05; **) p<0.01.
Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of... more Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of FFAs to growth media: 'FFA Secretion' and 'Green Recovery' from Synechocystis PCC 6803 cells. Text in red represents genetic modifications of SD100 strains.
Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumiga... more Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumigatus-SD257 pellets. 1) A. fumigatus-SD100 (left), A. fumigatus-SD256 after CO2 limitation (right); 2) A. fumigatus-SD100 (left), A. fumigatus-SD257 after CO2 limitation (right).
Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. f... more Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. fumigatus-Synechocystis PCC 6803 associations. (A,B) A. fumigatus pellets growing in liquid media; (C) A. fumigatus-SD100 and (D) A. fumigatus-SD216 pellets.
Additional file 10: Figure S9. Application of A. fumigatus-SD100 pellets for 25% swine wastewater... more Additional file 10: Figure S9. Application of A. fumigatus-SD100 pellets for 25% swine wastewater treatment. (1) 25% swine wastewater at t=0 and 48 h after treatment with SD100 (2); A. fumigatus (3) and A. fumigatus-SD (4). Tap water as control (5).
Additional file 3: Figure S3. Biofilms growth patterns. (A) Biofilm #52 is floating on the surfac... more Additional file 3: Figure S3. Biofilms growth patterns. (A) Biofilm #52 is floating on the surface of F2 medium (a) and attached to the glass walls (b, c). (B) Biofilms grown on agar plates. (h) image of Biofilm #52 under UV light. Scale bars: A (a, b, c),B (f),1 cm;B (a–e, g and h), 25 µm.
Additional file 13: Table S4. Chemical composition of ROC streams after treatment with assembled ... more Additional file 13: Table S4. Chemical composition of ROC streams after treatment with assembled biofilm.
Background Dinoflagellates are a ubiquitous and ecologically important component of marine phytop... more Background Dinoflagellates are a ubiquitous and ecologically important component of marine phytoplankton communities, with particularly notable species including those associated with harmful algal blooms (HABs) and those that bioluminesce. High-throughput sequencing offers a novel approach compared to traditional microscopy for determining species assemblages and distributions of dinoflagellates, which are poorly known especially in Australian waters. Results We assessed the composition of dinoflagellate assemblages in two Australian locations: coastal temperate Port Phillip Bay and offshore tropical waters of Davies Reef (Great Barrier Reef). These locations differ in certain environmental parameters reflecting latitude as well as possible anthropogenic influences. Molecular taxonomic assessment revealed more species than traditional microscopy, and it showed statistically significant differences in dinoflagellate assemblages between locations. Bioluminescent species and known ass...
White spot syndrome virus (WSSV) is the leading cause of shrimp mortality in farms all over the w... more White spot syndrome virus (WSSV) is the leading cause of shrimp mortality in farms all over the world. In Vietnam, for the last five to ten years, WSSV has always been among the top causes of diseases and loss in our shrimp aquaculture. VP28 and VP26 are two capsid proteins of WSSV that commonly used as biomarkers for diagnosis and target antigens for vaccine against WSSV. Recombinant VP28 (rVP28) has been studied and expressed in various expression systems including E. coli, yeast and baculovirus. rVP28 expressed in these systems showed effective protection against WSSV in shrimps, though there remains limitation on expression efficiency and safety. Recently, green microalgae Chlamydomonas reinhardtii has been widely used to express pharmaceutical proteins and edible vaccines for aquaculture thanks to its advantages as a safe and efficient host. C. reinhardtii is also used as nutrious natural food for shrimps due to its benefits towards shrimp health and growth. In this study, the ...
Fungal Applications in Sustainable Environmental Biotechnology, 2016
Microalgal applications represent potential green and smart solutions for the treatment of differ... more Microalgal applications represent potential green and smart solutions for the treatment of different types of wastewaters. Fungal–microalgal associations are gaining increasing attention as a low cost and an efficient strategy for the concentration of microalgal cells and the additive contribution of their components to the production of value-added chemicals and biofuels. In spite of the obvious attractiveness of microalgal-based bioremediation, there are still some challenges that can affect their economic viability. The costs associated with removing microalgal cells from treated wastewaters and harvesting them for the production of value-added products can account for up to 50 % of the total cost. With both biological components known to be involved in absorption of key nutrients and microelements from growing environments, fungal–microalgal consortiums show cumulative and synergistic effects on wastewater treatment efficiencies. This review covers the potential of microalgal representatives and their association with filamentous fungi for the treatment of different types of wastewaters and conversion of generated biomass into value-added chemicals and biofuels.
FtsZ1-1 and MinD plastid division-related genes were identified and cloned from Brassica oleracea... more FtsZ1-1 and MinD plastid division-related genes were identified and cloned from Brassica oleracea var. botrytis. Transgenic tobacco plants expressing BoFtsZ1-1 or BoMinD exhibited cells with either fewer but abnormally large chloroplasts or more but smaller chloroplasts relative to wild-type tobacco plants. An abnormal chloroplast phenotype in guard cells was found in BoMinD transgenic tobacco plants but not in BoFtsZ1-1 transgenic tobacco plants. Transgenic tobacco plants bearing the macro-chloroplast phenotype had 10 to 20-fold increased levels of total FtsZ1-1 or MinD, whilst the transgenic tobacco plants bearing the mini-chloroplast phenotype had lower increased FtsZ1-1 or absence of detectable MinD. We also described for the first time, plastid transformation of macro-chloroplast bearing tobacco shoots with a gene cassette allowing for expression of green fluorescent protein (GFP). Homoplasmic plastid transformants from normal chloroplast and macro-chloroplast tobacco plants expressing GFP were obtained. Both types of transformants accumulated GFP at ~6% of total soluble protein, thus indicating that cells containing macro-chloroplasts can regenerate shoots in tissue culture and can stably integrate and express a foreign gene to similar levels as plant cells containing a normal chloroplast size and number.
Generation of transgenic trees for reproductive incompetence is an important application of genet... more Generation of transgenic trees for reproductive incompetence is an important application of genetic engineering to commercial forestry. This involves isolation and characterisation of the regulatory regions of the genes expressed at the very early stages of male and female reproductive organ development. A gene family showing specific expression in Pinus radiata immature male and female cone-buds was isolated and characterised. A cell ablation strategy involving expression of toxin genes, under the control of the regulatory sequences of these genes, led to the ablation of the target cells. The major problem with this strategy was 'promoter leakage', low (background) levels of expression of some flower-specific promoters in vegetative tissues during flowering and non-flowering periods. To improve the cell ablation strategy, a cascade of chimeric genes was designed. During non-flowering, controlled expression of the anti-toxin gene from Bacillus amyloliquefaciens, Barstar, in non-flowering tissues arrested the accumulation of the toxic Barnase protein.
Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigat... more Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigatus-Synechocystis pellets after flocculation. (A) Increase in lipids in A. fumigatus/GLU-SD pellets compare to the additive yields of lipid/intracellular FFA and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. (B) Increase in lipids in A. fumigatus/TWS-SD pellets compare to the additive yields of lipid/intracellular FFAs and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. Significance levels: *) p<0.05; **) p<0.01.
Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of... more Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of FFAs to growth media: 'FFA Secretion' and 'Green Recovery' from Synechocystis PCC 6803 cells. Text in red represents genetic modifications of SD100 strains.
Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumiga... more Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumigatus-SD257 pellets. 1) A. fumigatus-SD100 (left), A. fumigatus-SD256 after CO2 limitation (right); 2) A. fumigatus-SD100 (left), A. fumigatus-SD257 after CO2 limitation (right).
Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. f... more Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. fumigatus-Synechocystis PCC 6803 associations. (A,B) A. fumigatus pellets growing in liquid media; (C) A. fumigatus-SD100 and (D) A. fumigatus-SD216 pellets.
Additional file 10: Figure S9. Application of A. fumigatus-SD100 pellets for 25% swine wastewater... more Additional file 10: Figure S9. Application of A. fumigatus-SD100 pellets for 25% swine wastewater treatment. (1) 25% swine wastewater at t=0 and 48 h after treatment with SD100 (2); A. fumigatus (3) and A. fumigatus-SD (4). Tap water as control (5).
Additional file 3: Figure S3. Biofilms growth patterns. (A) Biofilm #52 is floating on the surfac... more Additional file 3: Figure S3. Biofilms growth patterns. (A) Biofilm #52 is floating on the surface of F2 medium (a) and attached to the glass walls (b, c). (B) Biofilms grown on agar plates. (h) image of Biofilm #52 under UV light. Scale bars: A (a, b, c),B (f),1 cm;B (a–e, g and h), 25 µm.
Additional file 13: Table S4. Chemical composition of ROC streams after treatment with assembled ... more Additional file 13: Table S4. Chemical composition of ROC streams after treatment with assembled biofilm.
Background Dinoflagellates are a ubiquitous and ecologically important component of marine phytop... more Background Dinoflagellates are a ubiquitous and ecologically important component of marine phytoplankton communities, with particularly notable species including those associated with harmful algal blooms (HABs) and those that bioluminesce. High-throughput sequencing offers a novel approach compared to traditional microscopy for determining species assemblages and distributions of dinoflagellates, which are poorly known especially in Australian waters. Results We assessed the composition of dinoflagellate assemblages in two Australian locations: coastal temperate Port Phillip Bay and offshore tropical waters of Davies Reef (Great Barrier Reef). These locations differ in certain environmental parameters reflecting latitude as well as possible anthropogenic influences. Molecular taxonomic assessment revealed more species than traditional microscopy, and it showed statistically significant differences in dinoflagellate assemblages between locations. Bioluminescent species and known ass...
White spot syndrome virus (WSSV) is the leading cause of shrimp mortality in farms all over the w... more White spot syndrome virus (WSSV) is the leading cause of shrimp mortality in farms all over the world. In Vietnam, for the last five to ten years, WSSV has always been among the top causes of diseases and loss in our shrimp aquaculture. VP28 and VP26 are two capsid proteins of WSSV that commonly used as biomarkers for diagnosis and target antigens for vaccine against WSSV. Recombinant VP28 (rVP28) has been studied and expressed in various expression systems including E. coli, yeast and baculovirus. rVP28 expressed in these systems showed effective protection against WSSV in shrimps, though there remains limitation on expression efficiency and safety. Recently, green microalgae Chlamydomonas reinhardtii has been widely used to express pharmaceutical proteins and edible vaccines for aquaculture thanks to its advantages as a safe and efficient host. C. reinhardtii is also used as nutrious natural food for shrimps due to its benefits towards shrimp health and growth. In this study, the ...
Fungal Applications in Sustainable Environmental Biotechnology, 2016
Microalgal applications represent potential green and smart solutions for the treatment of differ... more Microalgal applications represent potential green and smart solutions for the treatment of different types of wastewaters. Fungal–microalgal associations are gaining increasing attention as a low cost and an efficient strategy for the concentration of microalgal cells and the additive contribution of their components to the production of value-added chemicals and biofuels. In spite of the obvious attractiveness of microalgal-based bioremediation, there are still some challenges that can affect their economic viability. The costs associated with removing microalgal cells from treated wastewaters and harvesting them for the production of value-added products can account for up to 50 % of the total cost. With both biological components known to be involved in absorption of key nutrients and microelements from growing environments, fungal–microalgal consortiums show cumulative and synergistic effects on wastewater treatment efficiencies. This review covers the potential of microalgal representatives and their association with filamentous fungi for the treatment of different types of wastewaters and conversion of generated biomass into value-added chemicals and biofuels.
Uploads
Papers by Aidyn Mouradov