Triterpenoid saponins from the roots of Panax notoginseng with protective effects against APAP-induced liver injury

Fitoterapia. 2024 Aug 8:106159. doi: 10.1016/j.fitote.2024.106159. Online ahead of print.

Authors

Yungang Tian¹, Jingjing Fan², Rongshen Wang³, Rui Ding⁴, Chunxue Zhao², Jia Xu², Qingyun Zhang², Tiegui Nan⁵, Xianchan Li⁶, Min Ye⁷

Affiliations

¹ School of Pharmacy, Minzu University of China, 27 Zhongguancun South Street, Beijing 100081, China; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
² State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
³ State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China; School of Pharmacy, Weifang Medical University, 7166 Baotong West Street, Weifang City, Shandong 261053, China.
⁴ Ningxia Institute for Drug Control, No. 163, Fengyue Road, Jinfeng District, Yinchuan 750002, China.
⁵ State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijng 100700, China. Electronic address: nantiegui@163.com.
⁶ School of Pharmacy, Minzu University of China, 27 Zhongguancun South Street, Beijing 100081, China; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Electronic address: xcli@bjmu.edu.cn.
⁷ State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijng 100700, China; Peking University-Yunnan Baiyao International Medical Research Center, 38 Xueyuan Road, Beijing 100191, China. Electronic address: yemin@bjmu.edu.cn.

PMID: 39127307
DOI: 10.1016/j.fitote.2024.106159

Abstract

Five previously undescribed protopanaxatriol type saponins, notoginsenoside Ta-Te (1-5), together with eighteen known triterpenoid saponins (6-23) were isolated from the roots of Panax notoginseng. The structures of new compounds were determined by HRESIMS and NMR spectroscopic analyses and chemical methods. Compounds 1 and 2 were the first examples of ginsenosides featuring a 6-deoxy-β-d-glucose moiety from Panax species. Compounds 1-4, 7, 10, 12, 21-22 showed protective effects on L02 cells against the injury of acetaminophen (APAP). Among them, notoginsenoside R1 (12), ginsenoside Rg1 (21), and ginsenoside Re (22) were the most potent ones, with cell viabilities >80%. Moreover, compounds 12 and 22 remarkably alleviated APAP-induced liver injury in mice. These saponins are potential hepatoprotective agents.

Keywords: APAP-induced liver injury; Hepatoprotective effect; Panax notoginseng; Triterpenoid saponin.