The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.
Keywords: Conical tubes; Isolation; Laboratory budget; Plasmid DNA; Polypropylene; Polystyrene.
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