Abstract
Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics*
-
DNA Primers / genetics
-
Methicillin Resistance / genetics*
-
Penicillin-Binding Proteins
-
Reverse Transcriptase Polymerase Chain Reaction / methods*
-
Staphylococcus aureus / drug effects
-
Staphylococcus aureus / genetics*
Substances
-
Bacterial Proteins
-
DNA Primers
-
Penicillin-Binding Proteins
-
mecA protein, Staphylococcus aureus