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Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins

Cancer Res. 2001 May 1;61(9):3760-9.

Abstract

We have identified and cloned a novel gene, trp-p8, by screening a prostate-specific subtracted cDNA library. The 5694-bp cDNA has a 3312-bp open reading frame, which codes for a 1104 amino acid putative protein with seven transmembrane domains. The predicted protein revealed significant homology with the transient receptor potential (trp) family of Ca(2+) channel proteins. Northern blot analysis indicated that trp-p8 expression within normal human tissues is mostly restricted to prostate epithelial cells. In situ hybridization analysis showed that trp-p8 mRNA expression was at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biomarkers, Tumor / biosynthesis
  • Biomarkers, Tumor / genetics
  • COS Cells
  • Calcium Channels / biosynthesis
  • Calcium Channels / genetics*
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / genetics
  • DNA, Neoplasm / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • In Situ Hybridization
  • Ion Channels*
  • Male
  • Molecular Sequence Data
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics*
  • Organ Specificity
  • Prostate / metabolism
  • Prostate / physiology*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • TRPM Cation Channels
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Biomarkers, Tumor
  • Calcium Channels
  • DNA, Complementary
  • DNA, Neoplasm
  • Ion Channels
  • Neoplasm Proteins
  • RNA, Messenger
  • TRPM Cation Channels
  • TRPM8 protein, human