Telomere holds special mechanism for solving end repair problems and maintaining genomic stabilit... more Telomere holds special mechanism for solving end repair problems and maintaining genomic stability. Protection of telo-
meres 1 (POT1) which belongs to shelterin family is identified as a key protein that recruits telomerase by interacting with telomere repeat binding factors (TRB1-3). Since, deciphering the mechanism through which POT assembles telomerase is of great interest, computational approaches have been undertaken to understand the mechanism in a well developed model system – Arabidopsis thaliana. As a first step, an untraditional approach was mediated to locate the active site residues on modeled AtPOT1b protein by interaction studies using molecular docking. To keep in trend with the recent developments, peptide construction and validation was promoted as the next step via molecular dynamics simulation studies. Finally, the validated peptides based on propensity score was evaluated for its efficacy as a potent inhibitor for POT and TRB1-3 interactions. The best peptide, namely, (1-2-d) out of 30 designed peptides, was proved to be vital inhibitor by weakening the interacting complexes.
POT from Arabidopsis thaliana is a member of shelterin complex and belongs to Telo bind protein f... more POT from Arabidopsis thaliana is a member of shelterin complex and belongs to Telo bind protein family. hree homologs are reported, namely, AtPOT1a, AtPOT1b, and AtPOT1c, where AtPOT1b is involved in genomic stability and chromosome end protection by providing necessary grip to G-rich region of telomeric DNA for telomerase assembly. Telomeric binding factors (TRB1–3) physically interact with POT with no known functionality. In this work attempt has been made to elucidate the reason behind the interaction by analyzing molecular docking interaction between AtPOT1b and AtTRB1–3, which yielded potential residues, which could play essential role in structuralmodiication. 3 nsmolecular simulation helped to look into structural stability
and conformational dynamics portraying domain movements. AtTRB’s interaction with AtPOT1b provoked structural changes in AtPOT1b, thereby increasing the ainity for single strandDNA(ssDNA) as compared to double strandDNA(dsDNA). Although the obtained results require experimental evidence they can act as a guide in tracing the functions in other organisms.he information provided in this paper would be helpful in understanding functions of TRB1–3 with respect to genomic stability.
Plants in nature may face a wide range of favorable or unfavorable biotic and abiotic factors dur... more Plants in nature may face a wide range of favorable or unfavorable biotic and abiotic factors during their life cycle. Any of these factors may cause stress in plants; therefore, they have to be more adaptable to stressful environments and must acquire greater response to diferent stresses.he objective of this study is to retrieve and arrange data fromthe literature in a standardized electronic format for the development of information resources on potential stress responsive genes in Arabidopsis thaliana.his provides a powerful mean for manipulation, comparison, search, and retrieval of records describing the nature of various stress responsive genes in Arabidopsis thaliana.he database is based exclusively on published stress tolerance genes associated with plants.
Telomere holds special mechanism for solving end repair problems and maintaining genomic stabilit... more Telomere holds special mechanism for solving end repair problems and maintaining genomic stability. Protection of telo-
meres 1 (POT1) which belongs to shelterin family is identified as a key protein that recruits telomerase by interacting with telomere repeat binding factors (TRB1-3). Since, deciphering the mechanism through which POT assembles telomerase is of great interest, computational approaches have been undertaken to understand the mechanism in a well developed model system – Arabidopsis thaliana. As a first step, an untraditional approach was mediated to locate the active site residues on modeled AtPOT1b protein by interaction studies using molecular docking. To keep in trend with the recent developments, peptide construction and validation was promoted as the next step via molecular dynamics simulation studies. Finally, the validated peptides based on propensity score was evaluated for its efficacy as a potent inhibitor for POT and TRB1-3 interactions. The best peptide, namely, (1-2-d) out of 30 designed peptides, was proved to be vital inhibitor by weakening the interacting complexes.
POT from Arabidopsis thaliana is a member of shelterin complex and belongs to Telo bind protein f... more POT from Arabidopsis thaliana is a member of shelterin complex and belongs to Telo bind protein family. hree homologs are reported, namely, AtPOT1a, AtPOT1b, and AtPOT1c, where AtPOT1b is involved in genomic stability and chromosome end protection by providing necessary grip to G-rich region of telomeric DNA for telomerase assembly. Telomeric binding factors (TRB1–3) physically interact with POT with no known functionality. In this work attempt has been made to elucidate the reason behind the interaction by analyzing molecular docking interaction between AtPOT1b and AtTRB1–3, which yielded potential residues, which could play essential role in structuralmodiication. 3 nsmolecular simulation helped to look into structural stability
and conformational dynamics portraying domain movements. AtTRB’s interaction with AtPOT1b provoked structural changes in AtPOT1b, thereby increasing the ainity for single strandDNA(ssDNA) as compared to double strandDNA(dsDNA). Although the obtained results require experimental evidence they can act as a guide in tracing the functions in other organisms.he information provided in this paper would be helpful in understanding functions of TRB1–3 with respect to genomic stability.
Plants in nature may face a wide range of favorable or unfavorable biotic and abiotic factors dur... more Plants in nature may face a wide range of favorable or unfavorable biotic and abiotic factors during their life cycle. Any of these factors may cause stress in plants; therefore, they have to be more adaptable to stressful environments and must acquire greater response to diferent stresses.he objective of this study is to retrieve and arrange data fromthe literature in a standardized electronic format for the development of information resources on potential stress responsive genes in Arabidopsis thaliana.his provides a powerful mean for manipulation, comparison, search, and retrieval of records describing the nature of various stress responsive genes in Arabidopsis thaliana.he database is based exclusively on published stress tolerance genes associated with plants.
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Papers by Amit Jaiswal
meres 1 (POT1) which belongs to shelterin family is identified as a key protein that recruits telomerase by interacting with telomere repeat binding factors (TRB1-3). Since, deciphering the mechanism through which POT assembles telomerase is of great interest, computational approaches have been undertaken to understand the mechanism in a well developed model system – Arabidopsis thaliana. As a first step, an untraditional approach was mediated to locate the active site residues on modeled AtPOT1b protein by interaction studies using molecular docking. To keep in trend with the recent developments, peptide construction and validation was promoted as the next step via molecular dynamics simulation studies. Finally, the validated peptides based on propensity score was evaluated for its efficacy as a potent inhibitor for POT and TRB1-3 interactions. The best peptide, namely, (1-2-d) out of 30 designed peptides, was proved to be vital inhibitor by weakening the interacting complexes.
and conformational dynamics portraying domain movements. AtTRB’s interaction with AtPOT1b provoked structural changes in AtPOT1b, thereby increasing the ainity for single strandDNA(ssDNA) as compared to double strandDNA(dsDNA). Although the obtained results require experimental evidence they can act as a guide in tracing the functions in other organisms.he information provided in this paper would be helpful in understanding functions of TRB1–3 with respect to genomic stability.
meres 1 (POT1) which belongs to shelterin family is identified as a key protein that recruits telomerase by interacting with telomere repeat binding factors (TRB1-3). Since, deciphering the mechanism through which POT assembles telomerase is of great interest, computational approaches have been undertaken to understand the mechanism in a well developed model system – Arabidopsis thaliana. As a first step, an untraditional approach was mediated to locate the active site residues on modeled AtPOT1b protein by interaction studies using molecular docking. To keep in trend with the recent developments, peptide construction and validation was promoted as the next step via molecular dynamics simulation studies. Finally, the validated peptides based on propensity score was evaluated for its efficacy as a potent inhibitor for POT and TRB1-3 interactions. The best peptide, namely, (1-2-d) out of 30 designed peptides, was proved to be vital inhibitor by weakening the interacting complexes.
and conformational dynamics portraying domain movements. AtTRB’s interaction with AtPOT1b provoked structural changes in AtPOT1b, thereby increasing the ainity for single strandDNA(ssDNA) as compared to double strandDNA(dsDNA). Although the obtained results require experimental evidence they can act as a guide in tracing the functions in other organisms.he information provided in this paper would be helpful in understanding functions of TRB1–3 with respect to genomic stability.