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Fields, 1993 - Google Patents

The two-hybrid system to detect protein-protein interactions

Fields, 1993

Document ID
7220883940517315199
Author
Fields S
Publication year
Publication venue
Methods

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The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. In this method, genes encoding two hybrid proteins are constructed: one hybrid consists of the DNA-binding domain of a transcriptional activator fused to some protein" X"; …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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