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Schedl et al., 1973 - Google Patents

Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA

Schedl et al., 1973

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Document ID
4150138235096882367
Author
Schedl P
Primakoff P
Publication year
Publication venue
Proceedings of the National Academy of Sciences

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Using a simple three-step procedure, we have isolated thermosensitive mutants of E. coli that are specifically defective in transfer RNA (tRNA) synthesis. Our procedure was designed to identify mutants that are unable to make su3+ tRNATyr or grow at the restrictive …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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