Kinosita Jr et al., 1988 - Google Patents
Submicrosecond imaging under a pulsed-laser fluorescence microscopeKinosita Jr et al., 1988
- Document ID
- 13244478297830836832
- Author
- Kinosita Jr K
- Ashikawa I
- Hibino M
- Shigemori M
- Itoh H
- Nagayama K
- et al.
- Publication year
- Publication venue
- Time-Resolved Laser Spectroscopy in Biochemistry
External Links
Snippet
A microscope system has been constructed that enables digital imaging of a fluorescent cell under pulsed illumination. Each image is produced by a single laser pulse of duration less than 0.3 11 s. With this system, microsecond responses of a single cell to an externally …
- 238000003384 imaging method 0 title abstract description 23
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kinosita et al. | Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope | |
| Gabriel et al. | Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane | |
| Klenchin et al. | Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis | |
| Marszalek et al. | Schwan equation and transmembrane potential induced by alternating electric field | |
| Teruel et al. | Electroporation-induced formation of individual calcium entry sites in the cell body and processes of adherent cells | |
| CA1248045A (en) | Device and process for inducing membrane fusion under an electric field | |
| Zimmermann et al. | Cells with manipulated functions: new perspectives for cell biology, medicine, and technology | |
| Hibino et al. | Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential | |
| Rols et al. | Electropermeabilization of mammalian cells. Quantitative analysis of the phenomenon | |
| Vernier et al. | Nanoelectropulse-driven membrane perturbation and small molecule permeabilization | |
| Chang | Cell poration and cell fusion using an oscillating electric field | |
| Hibino et al. | Time courses of cell electroporation as revealed by submicrosecond imaging of transmembrane potential | |
| Hagiwara et al. | The anomalous rectification and cation selectivity of the membrane of a starfish egg cell | |
| Dimova et al. | Giant vesicles in electric fields | |
| Kinosita et al. | Events of membrane electroporation visualized on a time scale from microsecond to seconds | |
| US8465955B2 (en) | Method for electroporation of biological cells | |
| Prausnitz et al. | Millisecond measurement of transport during and after an electroporation pulse | |
| Sözer et al. | Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization | |
| Weaver et al. | Progress toward a theoretical model for electroporation mechanism: membrane electrical behavior and molecular transport | |
| Fisher et al. | Osmotic control of bilayer fusion | |
| Iwasa et al. | Fast in vitro movement of outer hair cells in an external electric field: effect of digitonin, a membrane permeabilizing agent | |
| Kinosita Jr et al. | Submicrosecond imaging under a pulsed-laser fluorescence microscope | |
| Zimmermann et al. | [16] Principles of electrofusion and electropermeabilization | |
| Yaoita et al. | Potential-controlled morphological change and lysis of HeLa cells cultured on an electrode surface | |
| Sowers | The mechanism of electroporation and electrofusion in erythrocyte membranes |