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Zenke et al., 2008 - Google Patents

Novel fugu U6 promoter driven shRNA expression vector for efficient vector based RNAi in fish cell lines

Zenke et al., 2008

Document ID
11916818039228755724
Author
Zenke K
Kim K
Publication year
Publication venue
Biochemical and Biophysical Research Communications

External Links

Snippet

In fish species, although many studies on the use of RNA interference (RNAi) for gene function analysis have been reported, almost of them have utilized in vitro transcribed or synthesized small interfering RNA (siRNA), and there are a few studies which examined …
Continue reading at www.sciencedirect.com (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/141—MicroRNAs, miRNAs
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