WO2015172573A1 - 一种鳗鲡早期仔鱼的开口饵料及其培育方法 - Google Patents
一种鳗鲡早期仔鱼的开口饵料及其培育方法 Download PDFInfo
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- WO2015172573A1 WO2015172573A1 PCT/CN2015/000076 CN2015000076W WO2015172573A1 WO 2015172573 A1 WO2015172573 A1 WO 2015172573A1 CN 2015000076 W CN2015000076 W CN 2015000076W WO 2015172573 A1 WO2015172573 A1 WO 2015172573A1
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- jellyfish
- larvae
- early
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- bait
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 241000252073 Anguilliformes Species 0.000 title abstract 2
- 238000012258 culturing Methods 0.000 title abstract 2
- 241000242583 Scyphozoa Species 0.000 claims abstract description 82
- 208000035404 Autolysis Diseases 0.000 claims abstract description 17
- 206010057248 Cell death Diseases 0.000 claims abstract description 17
- 230000028043 self proteolysis Effects 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 210000001519 tissue Anatomy 0.000 claims description 11
- 230000001939 inductive effect Effects 0.000 claims description 3
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- 231100000611 venom Toxicity 0.000 claims description 2
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- 239000011521 glass Substances 0.000 description 7
- 230000012447 hatching Effects 0.000 description 6
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- 241000251730 Chondrichthyes Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
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- 230000001079 digestive effect Effects 0.000 description 3
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- 235000015097 nutrients Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 241001474374 Blennius Species 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000034994 death Effects 0.000 description 2
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- 238000004519 manufacturing process Methods 0.000 description 2
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229920001592 potato starch Polymers 0.000 description 2
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- 241000238557 Decapoda Species 0.000 description 1
- 241000239366 Euphausiacea Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241001098499 Lanceolata Species 0.000 description 1
- 244000236658 Paeonia lactiflora Species 0.000 description 1
- 235000008598 Paeonia lactiflora Nutrition 0.000 description 1
- 241000048273 Periplaneta japonica Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
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- 230000006978 adaptation Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
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- 230000002358 autolytic effect Effects 0.000 description 1
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- 241001233061 earthworms Species 0.000 description 1
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- 239000004467 fishmeal Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 239000002994 raw material Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Definitions
- the invention belongs to the field of fish seedling cultivation, and particularly relates to an open bait for early larvae and a method for cultivating the same.
- squid The scientific name of squid is ⁇ , its nutritional value is very high, so it is called soft gold in the water.
- the growth process of earthworms is extremely peculiar. First, it lays eggs in seawater and then grows into fresh water. At present, the Japanese carp cultured in China, Japan and South Korea all rely on the capture of wild seedlings, and the seedling resources are declining year by year due to overfishing. Moreover, due to the problems of cultivating technology, the yield of wild seedlings caught is low, which also causes further waste of resources.
- the study of artificial reproduction of squid has always been a problem in the international aquatic biology community, so it is called the Goldbach conjecture in biology. .
- the Aquaculture Department of Japan is the main ingredient in the shark yolk freeze-dried powder, and the krill or other fresh fish and shrimp extract is added to make a paste-like feed for the cultivation of the hatched young fish (the early larvae), which has been successfully obtained.
- Willow scorpion and eventually obtained a small amount of squid adult (white peony, also known as glass enamel).
- the main problem of artificial breeding is the early hatching of the early larvae (ie, from the fertilized eggs by 3-5).
- the choice of the open bait for the early hatching of the larvae in the day is not suitable for the early larvae because the digestive system of the early larvae is not yet complete and the digestive capacity is weak.
- the present invention provides a method for cultivating an easily absorbed nutrient such as a jellyfish by using jellyfish as a material, utilizing the autolytic property of jellyfish, inducing autolysis of jellyfish, and releasing self-dissolving of jellyfish, thereby solving the early larvae (generally The larvae that are hatched from fertilized eggs for 3-5 days to 1-19 days after filming do not have the ability to digest and fail to feed early larvae.
- the technical proposal of the present invention is to provide an open bait for an early larvae, the bait comprising marine organisms having autolysis characteristics, preferably the jellyfish is selected as the main component of the open bait, and the jellyfish autolysate and extract can also be used. Wait as the main ingredient to feed the early larvae.
- the present invention also provides a method for cultivating early larvae by using the above-mentioned open bait, which is: (1) selecting a living wild or artificially cultured jellyfish to remove the toxic tentacles of the jellyfish; and (2) loading the jellyfish into the plastic.
- the jellyfish will die due to lack of oxygen, and the autolysis will occur within a few hours (1 to 5 hours) after the jellyfish dies; (3)
- the early larvae yolk
- the early larvae yolk
- the sac has not disappeared in the pool of early larvae. Pay attention to changing the water to maintain the water quality: If the feeding time is too early, you can remove the jellyfish tissue that has not been autolyzed and feed it again after changing the water until the early larvae start to feed the jellyfish in the autolysis state.
- the early larvae can be domesticated, and after the above steps, the fresh jellyfish (including the jellyfish whole or tissue blocks) or the living jellyfish or jellyfish living tissue can be fed. Since the juveniles have sharp teeth, they can directly feed on the living jellyfish tissue after domestication. Because living tissue is not easy to rot, feeding management is more convenient.
- the material fed by the present invention has self-dissolving properties relative to the method of feeding the hatched juvenile fish with shark yolk.
- the early development of the digestive system of the larvae is extremely imperfect, and the ability to digest protein and other nutrients is very weak.
- the jellyfish can be autolyzed and have low requirements on the digestive system, adapting to the physiological characteristics of the early larvae. At the same time, the jellyfish texture is soft and easy for early larvae to feed.
- the method of the invention can also continue to be used for the cultivation of canker-like larvae of alfalfa.
- the jellyfish release peptides, nucleotides, polysaccharides and other "flavoring substances", which can induce the early larvae to feed and enhance the feeding effect.
- the early larvae and willow-like larvae can be slowly domesticated to receive fresh jellyfish.
- the physiological characteristics of early hatching larvae are different from those of common fish seedlings. Because the digestive organs of the early larvae are basically not developed, the digestive enzymes are extremely inactive and cannot digest complex nutrients such as proteins and fats. After the jellyfish is autolyzed, macromolecules such as proteins, fats, nucleic acids, and mucopolysaccharides are hydrolyzed into small molecules by their own digestive enzymes, and the seedlings can be absorbed without digestion. The living jellyfish will be self-dissolved in the digestive cavity of the juvenile fish by the seedlings of the alfalfa, and they can be well absorbed and utilized.
- Tools gloves, scissors, plastic bags or storage bags, other materials needed to cultivate seedlings (oxygen pumps, artificial sea water, sinks for seedlings, etc.).
- Cultivation method (1) remove the venomous thorns of the jellyfish. Jellyfish have thorns, grow on the edge of the umbrella-shaped head, and will lick the larvae, so they should be removed. Wear gloves and cut all the thorns with scissors. To ensure that the cuts are clean, you can also cut off a part of the jellyfish cover.
- Jellyfish will self-dissolve after death, so it is only necessary to kill the jellyfish to induce autolysis.
- the jellyfish is sealed in a plastic bag or a plastic wrap bag, causing the jellyfish to suffocate and die. After a few hours (1 to 5 hours), the jellyfish is autolyzed. Pay attention to the autolysis process. When autolysis is found, you can take out the seedlings. If it is taken too late, it will lead to excessive autolysis and turn into a pool of water. It is easy to use and it is easy to pollute the water.
- the bait of the present invention may also have the following alternative forms: 1. pulverized jellyfish or jellyfish autolysate 80% to 90% by weight and potato starch 10% to 20%; or pulverized jellyfish Or jellyfish autolysate 90% ⁇ 98% and seaweed gum 2% ⁇ 10%, the production of frozen gelatinous solids, this method can reduce the water pollution of jellyfish autolysis, reduce the amount of water replacement.
- the pulverized jellyfish or jellyfish autolysate is 50% to 80%, the fermented soybean meal or MSG residue is 10% to 30%, and the potato starch is 10% to 20%.
- the paste-like feed pellet produced by this method has a good attracting effect. Not easily soluble in water.
- the pulverized jellyfish or jellyfish autolysate is 60%-80%, the fermented soybean meal or MSG residue is 15%-30%, and the seaweed gum is 2%-10%.
- the jelly-like feed pellet produced by this method has good attracting effect. Not easily soluble in water.
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
公开了一种鳗鲡早期仔鱼的开口饵料及使用该饵料的培育方法，其中的饵料中包括具有自溶特性的海洋生物如水母。
Description
本发明属于鱼类幼苗培育领域，特别涉及一种鳗鲡早期仔鱼的开口饵料及使用该饵料的培育方法。
鳗鱼的学名叫鳗鲡，其营养价值非常高，所以被称作是水中的软黄金。鳗鲡的生长过程极为奇特，先是在海水中产卵成苗，后又进入淡水成长。目前，中国、日本和韩国养殖的日本鳗鲡全部依赖捕捞野生苗，由于捕捞过度，鳗苗资源逐年衰退。并且，由于培育技术的诸多问题，造成捕捞的野生苗得出的产量偏低，也对资源造成了进一步的浪费。鳗鱼的人工繁殖研究，一直是国际水产生物学界的难题，所以被称作生物学中的哥德巴赫猜想。。
日本水产厅养殖研究所以鲨鱼卵黄冷冻干燥粉末为主要成分，并加入磷虾或其他鲜鱼虾抽提液制成糊状饲料，用于培育鳗鲡初孵幼鱼(鳗鲡早期仔鱼)，成功获得了柳叶鳗，并最终获得了少量的鳗鱼成体(白仔鳗，亦称玻璃鳗)。
但是，这种日本鳗鲡人工培育还存在以下不足：1.从受精卵孵化至早期仔鱼的鳗苗的成活率只有0～5％。2.从孵化的早期仔鱼至玻璃鳗苗养殖周期约需半年。3.第一代全人工孵化的玻璃鳗苗每尾成本达100万日元(约合人民币75000元)；第二代的玻璃鳗苗成本也需要几千日元(人民币几百元)。第二代鳗鲡全人工孵化成功仅仅是在实验室阶段，要实现规模生产还需要10年左右。短期而言，鳗鲡人工繁殖成功对国内外的鳗鱼产业界都没有实质影响。
其中，鳗鲡人工繁育主要问题在于初孵的早期仔鱼(即，从受精卵经3-5
天孵化的早期仔鱼)的开口饵料的选择，由于鳗鲡早期仔鱼消化系统尚不健全，消化能力弱，一般的鱼类开口饵料都不适合鳗鲡早期仔鱼。
发明内容
本发明为了解决上述问题，提供了通过使用水母为材料，利用水母的自溶特性，诱导水母自溶，水母自溶释放出多肽等易吸收的营养物质的培育方法，解决了鳗鲡早期仔鱼(一般为从受精卵经3-5天孵化至出膜后1-19天间的仔鱼)不具备消化能力、无法喂养鳗鲡早期仔鱼的问题。
本发明的技术方案为提供一种鳗鲡早期仔鱼的开口饵料，该饵料包括具有自溶特性的海洋生物，优选为该海洋生物选取水母作为开口饵料的主要成分，也可用水母自溶产物、提取液等作为主要成分饲喂鳗鲡早期仔鱼。
同时，本发明还提供了应用上述开口饵料培育鳗鲡早期仔鱼的方法，该方法为：(1)取材选用活体的野生或人工养殖的水母，去除水母的有毒触手；(2)将水母装入塑料袋或者保鲜袋中，使水母因缺乏氧气窒息死亡，水母死亡后几小时(1～5小时)会出现自溶；(3)当水母呈现半溶解的状态时，投喂鳗鲡鳗鲡早期仔鱼(卵黄囊未消失的鳗鲡早期仔鱼)的池中。注意勤换水保持水质：若饲喂时间偏早，可以捞出未自溶的水母组织并换水后再次投喂，直到鳗鲡早期仔鱼开始取食自溶状态的水母。
同时，还可对鳗鲡早期仔鱼进行驯化，经过上述步骤的后期可以投喂窒息的新鲜水母(包括水母全体或者组织块)、或活体水母或水母的活体组织。由于鳗鲡幼鱼牙齿尖利，经过驯化后可以直接取食活体水母组织。由于活体组织不易腐烂，饲养管理更方便。
本发明的优点：
技术层面：相对于以鲨鱼卵黄饲喂鳗鲡初孵幼鱼的方法，本发明饲喂的材料具有自我溶解的特性。鳗鲡早期仔鱼消化系统发育极度不完善，消化蛋白质等营养的能力很弱，水母可以自溶，对消化系统的要求低，适应了早期仔鱼的生理特性。同时水母质地柔软便于早期仔鱼取食。
应用层面：由于水母取材容易、成本低廉，用水母培育鳗鲡幼鱼的成本远远低于鲨鱼卵黄，具有可操作性和实用价值。
本发明的方法还可以继续用于鳗鲡的柳叶状幼体的培育。
优势：1.自溶后，水母释放出多肽、核苷酸、多糖等“呈味物质”，可以诱使鳗鲡早期仔鱼取食，增强饲喂的效果。后期适应后，可以慢慢驯化早期仔鱼、柳叶状幼体接受新鲜的水母。
2.初孵的鳗鲡早期仔鱼生理特性不同于普通鱼类幼苗。由于鳗鲡早期仔鱼消化器官基本没有发育，消化酶活力极差，不能消化蛋白质、脂肪等复杂营养物质。水母自溶后，蛋白质、脂肪、核酸、粘多糖等大分子物质都被自身消化酶水解为小分子物质，鳗鲡幼苗不需要消化即可吸收。活体的水母被鳗鲡幼苗取食后也会在鳗鲡幼鱼消化腔自溶，都能被很好地吸收利用。
因为水母含水量高、有毒，营养含量远低于鱼粉、卤虫、轮虫等常规开口饵料，一般生物不取食，因此现有技术中并没有水母作为鱼类的开口饵料的先例，将这样的材料用于鱼类育苗是本发明的首创。鳗鲡早期仔鱼和柳叶鳗没有肝脏、胰脏等消化器官，肠道为一根直管道，消化能力几乎没有，对于其的培育是鱼类幼苗培养的世界难题。而本发明通过长期试验得出了水母自溶的特性
恰好适合于鳗鲡早期仔鱼和柳叶鳗的结论，大大降低培育成本、并提高了幼苗存活比例，解决了这一世界难题。
以下实施方式仅用于阐述本发明，而本发明的保护范围并非仅仅局限于以下实施例。所述技术领域的普通技术人员依据本发明的公开内容，均可实现本发明的目的。
采用人工培养或者野生的活体水母、鳗鲡早期仔鱼(由受精卵孵化3～5天，卵黄囊接近消失状态的幼体)。
工具：手套、剪刀、塑料袋或者保鲜袋、其他培养鳗鲡幼苗所需的材料(氧气泵、人工海水、育苗用水槽等)。
培育方法：(1)去除水母有毒刺丝。水母都有刺丝，生长在伞状头部边缘，会蛰伤鳗鲡幼鱼，因此要除去。戴手套，用剪刀剪去全部刺丝，为确保剪除干净，可以把水母伞盖的一部分也剪掉。
(2)诱导水母自溶。水母死亡后都会自溶，因此只需处死水母，就可以诱导自溶的发生。将水母装进塑料袋或者保鲜薄膜袋中密封，使得水母窒息死亡，几小时后(1～5小时)水母就发生自溶现象。注意观察自溶过程，当发现出现自溶现象，就可以取出投喂鳗鲡幼苗了。若取出过迟，易导致过度自溶，化作一滩水，无法使用也容易污染水质。
(3)投喂鳗鲡早期仔鱼。将初步自溶的水母放入鳗鲡早期仔鱼池中。注意保持水质清洁，经常换水。为了确保鳗鲡早期仔鱼顺利开口取食，要在池中鳗鲡早期仔鱼卵黄囊未消失前饲喂。若饲喂时间偏早，可以捞出未自溶的水母组
织并换水后(已自溶部分的水母已经无法取出，只能换水)再次投喂。直到幼鱼开始取食自溶状态的水母。
(4)驯化。待鳗鲡幼鱼适应水母作为饵料后，可以投喂窒息的新鲜水母(包括水母全体或者组织块)，或进一步可以投喂去除了刺丝的活体水母或活体水母组织(活体水母切块后不会死亡，能够保持生命力)。饲喂活体水母或活体水母组织方便、成本低，幼鱼可以根据需要取食，经过取食后水母会在幼鱼消化道内自溶，不会污染水体。
(5)培育柳叶鳗。经过10～35天的饲喂，鳗鲡早期仔鱼长大，变成柳叶状幼体(即柳叶鳗)。柳叶状幼体的培育方法和早期仔鱼相同。
(6)培育玻璃鳗。经过3～12个月的饲养，当柳叶鳗长度在3～10厘米时，慢慢调低海水浓度，柳叶鳗就会变态发育为玻璃鳗。玻璃鳗按照鳗鲡常规饲养方式饲喂即可。
针对上述实例中使用的原料，本发明的饵料还可以有以下替代形式：1.粉碎的水母或水母自溶产物80％～90％(重量)和马铃薯淀粉10％～20％；或粉碎的水母或水母自溶产物90％～98％和海藻胶2％～10％，制成果冻样的凝胶状固体，此法可以减少水母自溶对水质的污染，减轻换水劳动量。
2.粉碎的水母或水母自溶产物50％～80％，发酵豆粕粉或味精渣10％～30％，马铃薯淀粉10％～20％，此法制作的糊状饲料团，诱食效果好，不易溶解于水中。
3.粉碎的水母或水母自溶产物60％～80％，发酵豆粕粉或味精渣15％～30％，海藻胶2％～10％，此法制作的果冻状饲料团，诱食效果好，不易溶解于水中。
Claims (10)
- 一种鳗鲡早期仔鱼的开口饵料，其特征在于所述饵料包括具有自溶特性的海洋生物。
- 根据权利要求1所述的鳗鲡早期仔鱼的开口饵料，其特征在于所述具有自溶特性的海洋生物为水母。
- 根据权利要求1或2所述的鳗鲡早期仔鱼的开口饵料，其特征在于所述饵料的主要成分为水母自溶产物或水母提取物。
- 一种鳗鲡早期仔鱼的培育方法，其特征在于所述方法为将具有自溶特性的海洋生物作为饵料的主要成分培育鳗鲡早期仔鱼。
- 根据权利要求4所述的鳗鲡早期仔鱼的培育方法，其特征在于所述具有自溶特性的海洋生物为水母。
- 根据权利要求4或5所述的鳗鲡早期仔鱼的培育方法，其特征在于所述饵料的主要成分为水母自溶产物或水母提取物。
- 根据权利要求4-6任一权利要求所述的鳗鲡早期仔鱼的培育方法，其特征在于所述方法具体包括如下步骤：(1)去除水母有毒刺丝；(2)诱导水母自溶：使步骤(1)所得水母窒息死亡，几小时后(1～5小时)水母就发生自溶现象；(3)投喂鳗鲡早期仔鱼：将步骤(2)所得初步自溶的水母放入鳗鲡早期仔鱼的池中。
- 根据权利要求7所述的鳗鲡早期仔鱼的培育方法，其特征在于所述方法还可进一步包括如下步骤：(4)驯化：待经过步骤(3)鳗鲡早期仔鱼适应水母作为饵料后，可以投喂窒息的新鲜水母(包括水母全体或者组织块)，或进一步可以投喂去除了刺丝的活体水母或活体水母组织。
- 根据权利要求7所述的鳗鲡早期仔鱼的培育方法，其特征在于步骤(3)中，步骤(2)所得初步自溶的水母放入卵黄囊未消失的鳗鲡早期仔鱼的池中；若饲喂时间偏早，可以捞出未自溶的水母组织并换水后再次投喂，直到鳗鲡早期仔鱼开始取食自溶状态的水母。
- 据权利要求4-10任一权利要求所述的鳗鲡早期仔鱼的培育方法，其特征在于该方法还可以用于培育鳗鲡的柳叶状幼体。
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| WO1995022893A2 (en) * | 1994-02-28 | 1995-08-31 | Specialty Marine Feeds Inc. | Euphausiid harvesting and processing method and apparatus |
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| CN1042049A (zh) * | 1989-10-09 | 1990-05-16 | 青海省复合饲料厂 | 鳗鲡高蛋白、脂肪的全价饲料 |
| KR100585500B1 (ko) * | 2005-01-27 | 2006-06-07 | 코미뱀부인터내셔날(주) | 양식 민물장어 사료첨가제 및 이것을 함유한 양식민물장어 사료 |
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