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WO2014082544A1 - Entacapone for prevention and treatment of obesity and related metabolic diseases - Google Patents

Entacapone for prevention and treatment of obesity and related metabolic diseases Download PDF

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Publication number
WO2014082544A1
WO2014082544A1 PCT/CN2013/087581 CN2013087581W WO2014082544A1 WO 2014082544 A1 WO2014082544 A1 WO 2014082544A1 CN 2013087581 W CN2013087581 W CN 2013087581W WO 2014082544 A1 WO2014082544 A1 WO 2014082544A1
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WO
WIPO (PCT)
Prior art keywords
entacapone
fto
ldl
medicament
weight gain
Prior art date
Application number
PCT/CN2013/087581
Other languages
French (fr)
Inventor
Niu Huang
Gang ZHI
Jijie CHAI
Shiming PENG
Nannan HOU
Original Assignee
National Institute Of Biological Sciences, Beijing
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Filing date
Publication date
Application filed by National Institute Of Biological Sciences, Beijing filed Critical National Institute Of Biological Sciences, Beijing
Priority to CA2892902A priority Critical patent/CA2892902C/en
Priority to AU2013351676A priority patent/AU2013351676B2/en
Priority to NZ708610A priority patent/NZ708610A/en
Priority to CN201380071479.1A priority patent/CN104955451B/en
Priority to EP13858101.2A priority patent/EP2925312B1/en
Priority to US14/147,531 priority patent/US10004715B2/en
Publication of WO2014082544A1 publication Critical patent/WO2014082544A1/en
Priority to US16/018,095 priority patent/US10682329B2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/275—Nitriles; Isonitriles
    • A61K31/277—Nitriles; Isonitriles having a ring, e.g. verapamil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Definitions

  • Entacapone for prevention and treatment of obesity and related metabolic diseases
  • FTO protein is an a-ketoglutarate and iron (II) dependent nucleic acid
  • the invention provides methods and composition for inhibiting weight gain, wherein the methods are also applicable to promoting weight loss, reducing serum LDL, cholesterol, LDL-c, and/or triglycerides, and/or treating an obesity related or metabolic disease or ameliorating or reducing the pathology or severity of an obesity related or metabolic disease or a symptom of an obesity related or metabolic disease selected from diabetes,
  • hyperglycemia diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases.
  • the invention provides methods of inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, comprising administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide), or a pharmaceutically-acceptable salt thereof.
  • entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide)
  • the invention provides methods of inhibiting weight gain comprising administering to a person in need thereof an effective amount of entacapone in conjunction with one or more different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
  • the person meets one or more criteria, such as (a) is not diagnosed with Parkinson's disease; (b) is less than 50, 40 or 30 years old; (c) is obese or over-weight; (d) suffers from or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases; (e) has genotype: SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A); and/or (f) pathogenically expresses or over-expresses FTO or Fto.
  • an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases.
  • e has genotype: SNP rs7202116 (G), rsl42
  • the method further comprising detecting in the person one or more of the criteria, particularly genotype SNP rs7202116 (G), or over-expression of FTO or Fto.
  • the method further comprises detecting a resultant inhibition of weight gain, promotion of weight loss, and/or improvement or amelioration of one or more of the criteria.
  • the method further comprises administering to the person an effective amount of one or more additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
  • the invention provides pharmaceutical compositions comprising entacapone copackaged or coformulated with one or more of the additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
  • the subject methods and compositions employ entacapone dosages of 0.25-5g, or g/day, or 0.5-5 g, or g/day.
  • Figure 1 The chemical structure of entacapone.
  • Figure 3 The dose-dependent inhibition activity of entacapone on triglyceride synthesis in Huh-7 cell line.
  • the X-axis is the compound concentration.
  • the Y-axis is "readout" of the triglyceride concentration using dyeing method.
  • Figure 7 Effects of entacapone on adipose and hepatic tissues in rats.
  • Figure 8 The anti-atherosclerosis efficacy of entacapone in Ldlr-Deficient Mice, a and b.
  • the invention provides methods of inhibiting weight gain or promoting weight loss comprising administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide), or a pharmaceutically-acceptable salt thereof, and including stereoisomers, particularly E-Z isomers, including polymeric forms, particularly the A form of the E isomer (e.g.
  • the subject methods and compositions are also applicable to related indications: promoting weight loss, reducing serum LDL, cholesterol, LDL-c, and/or triglycerides, and/or treating an obesity related disease or ameliorating or reducing the pathology or severity of an obesity related disease or a symptom of an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases.
  • the invention provides methods and compositions wherein entacapone is copackaged, coformulated or coadministered with one or more different medicaments for, specific for, or indicated for these related indications, and the methods may further comprising detecting, determining or diagnosing in the person one or more of the indications, and/or detecting a resultant improvement or amelioration of corresponding condition or symptom in the person.
  • the invention provides methods of inhibiting weight gain comprising administering to a person in need thereof an effective amount of entacapone in conjunction with one or more different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
  • Preferred different medicaments for these indications include different anti-weight gain medicaments, particularly a food intake inhibitor and/or a food absorption inhibitor; particularly Orlistat, Sibutramine, Lorcaserin, Rimonabant, Metformin , Exenatide ,
  • Pramlintide or a pharmaceutically-acceptable salt thereof, and cholesterol lowering drugs, such as statins, including atorvastatin (Lipitor), fluvastatin (Lescol), lovastatin (Altoprev, Mevacor), pravastatin (Pravachol), rosuvastatin (Crestor), simvastatin (Zocor), or a pharmaceutically-acceptable salt thereof.
  • statins including atorvastatin (Lipitor), fluvastatin (Lescol), lovastatin (Altoprev, Mevacor), pravastatin (Pravachol), rosuvastatin (Crestor), simvastatin (Zocor), or a pharmaceutically-acceptable salt thereof.
  • the person meets one or more criteria indicative of the disclosed non-Parkinson's indication
  • the method may further comprising detecting in the person one or more of the criteria, and/or detecting a resultant inhibition of weight gain, promotion of weight loss and/or improvement or amelioration of one or more of such criteria.
  • Criteria indicative of the disclosed non-Parkinson's indication include wherein the person (a) is not suffering from, or is not diagnosed with Parkinson's disease or symptoms thereof or other prior indication for entacapone or is not suffering from, or is not diagnosed with any degenerative disease of the central nervous system; (b) is less than 50, 40 or 30 years old; (c) is over-weight (e.g. a BMI of 25-30) or obese (e.g.
  • a BMI of over 30 suffers from, or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases; or (e) has a genotype associated with obesity or pathogenic or medically-undesirable weight gain, such as SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A), or a surrogate or proxy SNP in linkage disequilibrium therewith (with respect to the correlative phenotype; see references below) and having a r 2 value greater than 0.5; and/or (f) pathogenically expresses or over-expresses FTO or Fto (e.g.
  • an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cere
  • Re rs7202116 G see e.g. Yang et al.
  • FTO genotype is associated with phenotypic variability of body mass index, Nature, Sep 16, 2012, doi: 10.1038/naturel l401 [epub]; re rs9939609 A, see e.g. Freathy RM, et al (2008).
  • "Common variation in the FTO gene alters diabetes-related metabolic traits to the extent expected, given its effect on BMI”. Diabetes 57 (5): 1419-26. doi: 10.2337/db07- 1466. PMC 3073395.
  • PMID 18346983; re rsl421085 C see e.g.
  • the person does not suffer from a pathogenic deficiency of L-DOPA (L-3,4-dihydroxyphenylalanine), does not indicate L-DOPA, and/or is not in need of L-DOPA (levopoda) or a dopaminergic agent, and/or the entacapone is not administered in conjunction with L-DOPA or a doaminergic agent.
  • L-DOPA L-3,4-dihydroxyphenylalanine
  • the method further comprises administering to the person an effective amount of one or more additional, different medicament for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
  • the invention provides pharmaceutical compositions comprising entacapone copackaged or coformulated with one or more of the additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly different anti- weight gain medicaments.
  • the entacapone and additional, different anti- weight gain medicaments may be coformulated, particularly in unit dosage form, or unit dosage forms of each may be copackaged in a multipack adapted for sequential use, such as blisterpack, comprising sheets of unit dosage forms. Exemplary coformulation and copackagings are shown in Table 2.
  • Table 2 Exemplary coformulation and copackagings. 1. Entacapone / Orlistat in 1000mg/120mg or 2000mg/120mg coformulated tablets.
  • Entacapone / Exenatide lOOOmg or 2000mg tablet / 250 mcg/mL solution (Byetta), copackaged
  • Entacapone / Exenatide lOOOmg or 2000mg tablet / suspension powder, ER 2 mg (Bydureon), copackaged
  • Entacapone / Pramlintide lOOOmg or 2000mg tablet / 600 mcg/mL (as acetate), copackaged
  • Entacapone / atorvastatin (Lipitor) in lOOOmg/lOmg coformulated tablets.
  • Entacapone / lovastatin (Altoprev, Mevacor) in lOOOmg/lOmg coformulated tablets.
  • Entacapone / cholestyramine (Prevalite, Questran) in 1000/5000mg copackaged powder.
  • Entacapone / colesevelam (Welchol) in 1000mg/625mg coformulated tablets.
  • Entacapone / fenofibrate (Lofibra, TriCor) in 1000mg/54mg coformulated tablets.
  • the coadmininistered drugs can act supplementally or synergistically to increase the potency compared with separate administration, and thereby also permit use reduced or otherwise suboptimal or subtherapeutic dosages, if not coadministered, while maintaining efficacy, such as 50%/50% and 25%/25% coformulations and copackagings of those shown in Table 2.
  • pharmaceutically acceptable salts is meant to include salts of the active compounds which are prepared with relatively nontoxic acids or bases, depending on the particular substituents found on the compounds described herein.
  • base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent.
  • pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amino, or magnesium salt, or a similar salt.
  • acid addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired acid, either neat or in a suitable inert solvent.
  • suitable inert solvent examples include those derived from inorganic acids like hydrochloric, hydrobromic, nitric, carbonic,
  • the neutral forms of the compounds may be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner.
  • the parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the invention.
  • the compounds may be in a prodrug form.
  • Prodrugs of the compounds are those compounds that undergo chemical changes under physiological conditions to provide the compounds of the invention.
  • prodrugs can be converted to the compounds of the invention by chemical or biochemical methods in an ex vivo environment.
  • prodrugs can be slowly converted to the compounds of the invention when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent.
  • Prodrugs are often useful because, in some situations, they may be easier to administer than the parent drug. They may, for instance, be more bioavailable by oral administration than the parent drug.
  • the prodrug may also have improved solubility in pharmacological compositions over the parent drug.
  • prodrug derivatives are known in the art, such as those that rely on hydrolytic cleavage or oxidative activation of the prodrug.
  • An example, without limitation, of a prodrug would be a compound of the invention which is administered as an ester (the "prodrug"), but then is metabolically hydrolyzed to the carboxylic acid, the active entity.
  • Subject compounds can exist in unsolvated forms as well as solvated forms, including hydrated forms. In general, the solvated forms are equivalent to unsolvated forms and are intended to be encompassed within the scope of the invention. Subject compounds may exist in multiple crystalline or amorphous forms. In general, all physical forms are equivalent for the uses contemplated and are intended to be within the scope of the invention.
  • compositions for administration can take the form of bulk liquid solutions or suspensions, or bulk powders. More commonly, however, the compositions are presented in unit dosage forms to facilitate accurate dosing.
  • unit dosage forms refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.
  • Typical unit dosage forms include prefilled, premeasured ampules or syringes of the liquid compositions or pills, tablets, capsules, losenges or the like in the case of solid compositions.
  • compositions are formulated or delivered in extended or controlled delivery systems, such as diffusion systems (e.g. reservoir devices, matrix devices, diffusion-controlled implants and transdermal patches) and encapsulated and matrix dissolution systems, erosion products, osmotic pump systems, ion exchange resins, etc.
  • diffusion systems e.g. reservoir devices, matrix devices, diffusion-controlled implants and transdermal patches
  • encapsulated and matrix dissolution systems erosion products, osmotic pump systems, ion exchange resins, etc.
  • the amount administered is far in excess of, at least 2, 2.5, 5 or 10X that (200mg) currently indicated for Parkinson's Disease, and will preferably be 0.5-10, 0.5-5, 0.5-2.5, 1-10, 1-5, 1-2.5, 2-10, or 2-5g/day, in unit dosage forms of 0.25, 0.5, 1, 1.5, 2 or 2.5g.
  • the compounds can be administered by a variety of methods including, but not limited to, parenteral, topical, oral, or local administration, such as by aerosol or
  • the therapeutic protocols e.g., dosage amounts and times of administration
  • the therapeutic protocols can be varied in view of the observed effects of the administered therapeutic agents on the patient, and in view of the observed responses of the disease to the administered therapeutic agents.
  • entacapone a COMT (Catechol-O- methyltransferase) inhibitor used for treating Parkinson disease.
  • adipose and hepatic tissues of rat in drug- treated group showed dramatic changes comparing to control group, with reduced size of adipose cells and reduced level of liver steatosis (Fig. 7 a,b H&E staining, 10 x
  • Table 1 Effects of Entacapone on serum biochemistry in rats. Difference is the percentage of concentration change of index in drug treatment group compared to that in control group.
  • anti-weight gain effective human dosages should be far in excess of the standard 200mg/day dosage for treating Parkinsons, preferably 0.5-10, 0.5-5, 0.5-2.5, 0.5-1, 1-10, 1-5, 1-2.5, 2-10, or 2-5g/day.
  • Gao X. et al.
  • the fat mass and obesity associated gene FTO functions in the brain to regulate postnatal growth in mice.

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Abstract

Weight gain is inhibited by administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2- enamide), or a pharmaceutically-acceptable salt thereof, particularly in conjunction with a second, different anti-weight gain medicament. Pharmaceutical compositions comprise entacapone copackaged or coformulated with a second, different anti-weight gain medicament.

Description

Entacapone for prevention and treatment of obesity and related metabolic diseases
Inventors: Huang Niu; Zhi Gang; Chai Jijie; Peng Shiming; Hou Nannan, all of Beijing, CN Assignee: National Institute of Biological Sciences, Beijing
[001] Introduction
[002] Obesity is becoming a severe health problem worldwide and many factors contribute to this chronic disease, including environmental factors and genetic factors. Recently, GWAS (genome-wide association studies) were applied to investigate patients with obesity and a single gene - FTO (fat mass and obesity) was identified to strongly associate with obesity.1"4 FTO's functional role in obesity was subsequently confirmed in transgenetic animal models, such as FTO knockout mouse, FTO-overexpression mouse and FTO-I367F mutation mouse.5" 8 More specifically, FTO global-knockout and neuron- specific knockout induce body weight loss5'6, while FTO gene overexpression results in obesity.8 One mis-sense mutation was observed to inhibit FTO enzymatic function and protect mouse from obesity.7 Nevertheless, FTO is expressed in many tissues, especially in hypothalamic nuclei controlling energy expenditure,9 which is consistent with the suggestion that FTO affects energy homeostasis.6
[003] FTO protein is an a-ketoglutarate and iron (II) dependent nucleic acid
demethylase.9 10 Its preferred substrate is N6-meA in message RNA, which locates near the stop codon and influences gene translation. 11 '12 However, its mode of mechanism is not clear, such as its upstream and downstream effectors and its regulation mechanism. It is desirable to identify small molecule, drug-like FTO inhibitors for studying FTO function and discovering novel anti-obesity agent.
[004] Existing drugs are approved for human use and have known safety profiles, so they can be rapidly evaluated for new indications of biological interest. Herein, we report the identification of a known FDA approved drug - Entacapone as FTO inhibitor using structure- based virtual screening method in combination with biological activity measurements, including enzymatic activity, cellular activity and in high-fat diet induced obesity (DIO) animal model.
[005] Summary of the Invention
[006] The invention provides methods and composition for inhibiting weight gain, wherein the methods are also applicable to promoting weight loss, reducing serum LDL, cholesterol, LDL-c, and/or triglycerides, and/or treating an obesity related or metabolic disease or ameliorating or reducing the pathology or severity of an obesity related or metabolic disease or a symptom of an obesity related or metabolic disease selected from diabetes,
hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases.
[007] In one aspect the invention provides methods of inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, comprising administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide), or a pharmaceutically-acceptable salt thereof.
[008] In another aspect the invention provides methods of inhibiting weight gain comprising administering to a person in need thereof an effective amount of entacapone in conjunction with one or more different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
[009] In particular embodiments thereof, the person meets one or more criteria, such as (a) is not diagnosed with Parkinson's disease; (b) is less than 50, 40 or 30 years old; (c) is obese or over-weight; (d) suffers from or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases; (e) has genotype: SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A); and/or (f) pathogenically expresses or over-expresses FTO or Fto.
[010] In particular embodiments the method further comprising detecting in the person one or more of the criteria, particularly genotype SNP rs7202116 (G), or over-expression of FTO or Fto.
[011] In particular embodiments the method further comprises detecting a resultant inhibition of weight gain, promotion of weight loss, and/or improvement or amelioration of one or more of the criteria.
[012] In particular embodiments, the method further comprises administering to the person an effective amount of one or more additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
[013] In another aspect the invention provides pharmaceutical compositions comprising entacapone copackaged or coformulated with one or more of the additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
[014] In particular embodiments the subject methods and compositions employ entacapone dosages of 0.25-5g, or g/day, or 0.5-5 g, or g/day.
[015] The invention encompasses all combination of the particular embodiments recited herein.
[016] Brief Description of the Figures
[017] Figure 1. The chemical structure of entacapone.
[018] Figure 2. The dose-dependent inhibition of entacapone against FTO protein measured in demethylation assay.
[019] Figure 3. The dose-dependent inhibition activity of entacapone on triglyceride synthesis in Huh-7 cell line. The X-axis is the compound concentration. The Y-axis is "readout" of the triglyceride concentration using dyeing method.
[020] Figure 4. Effects of entacapone on body weight in rats. (* p- value < 0.05)
[021] Figure 5. Effects of entacapone on food intake in rats.
[022] Figure 6. Effects of entacapone on LDL-c of serum in rats. (*** p-value < 0.005)
[023] Figure 7. Effects of entacapone on adipose and hepatic tissues in rats.
[024] Figure 8. The anti-atherosclerosis efficacy of entacapone in Ldlr-Deficient Mice, a and b. The Oil red O-stained lesion area in aortic sinuses of control group and drug-treated group, respectively, c. Quantitative analysis of lesional areas in the aortic sinuses of two groups.
[025] Figure 9. Effects of entacapone on body weight in obese mice. (* p-value < 0.05)
[026] Description of Particular Embodiments of the Invention
[027] In one aspect the invention provides methods of inhibiting weight gain or promoting weight loss comprising administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide), or a pharmaceutically-acceptable salt thereof, and including stereoisomers, particularly E-Z isomers, including polymeric forms, particularly the A form of the E isomer (e.g.
US5135950).
[028] The subject methods and compositions are also applicable to related indications: promoting weight loss, reducing serum LDL, cholesterol, LDL-c, and/or triglycerides, and/or treating an obesity related disease or ameliorating or reducing the pathology or severity of an obesity related disease or a symptom of an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases. Accordingly, the invention provides methods and compositions wherein entacapone is copackaged, coformulated or coadministered with one or more different medicaments for, specific for, or indicated for these related indications, and the methods may further comprising detecting, determining or diagnosing in the person one or more of the indications, and/or detecting a resultant improvement or amelioration of corresponding condition or symptom in the person.
[029] In one aspect the invention provides methods of inhibiting weight gain comprising administering to a person in need thereof an effective amount of entacapone in conjunction with one or more different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
[030] Preferred different medicaments for these indications include different anti-weight gain medicaments, particularly a food intake inhibitor and/or a food absorption inhibitor; particularly Orlistat, Sibutramine, Lorcaserin, Rimonabant, Metformin , Exenatide ,
Pramlintide, or a pharmaceutically-acceptable salt thereof, and cholesterol lowering drugs, such as statins, including atorvastatin (Lipitor), fluvastatin (Lescol), lovastatin (Altoprev, Mevacor), pravastatin (Pravachol), rosuvastatin (Crestor), simvastatin (Zocor), or a pharmaceutically-acceptable salt thereof.
[031] In particular embodiments thereof, the person meets one or more criteria indicative of the disclosed non-Parkinson's indication, and the method may further comprising detecting in the person one or more of the criteria, and/or detecting a resultant inhibition of weight gain, promotion of weight loss and/or improvement or amelioration of one or more of such criteria.
[032] Criteria indicative of the disclosed non-Parkinson's indication include wherein the person (a) is not suffering from, or is not diagnosed with Parkinson's disease or symptoms thereof or other prior indication for entacapone or is not suffering from, or is not diagnosed with any degenerative disease of the central nervous system; (b) is less than 50, 40 or 30 years old; (c) is over-weight (e.g. a BMI of 25-30) or obese (e.g. a BMI of over 30); (d) suffers from, or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases; or (e) has a genotype associated with obesity or pathogenic or medically-undesirable weight gain, such as SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A), or a surrogate or proxy SNP in linkage disequilibrium therewith (with respect to the correlative phenotype; see references below) and having a r2 value greater than 0.5; and/or (f) pathogenically expresses or over-expresses FTO or Fto (e.g. comprises and expresses a multi-copy fto gene). Re rs7202116 G, see e.g. Yang et al. , FTO genotype is associated with phenotypic variability of body mass index, Nature, Sep 16, 2012, doi: 10.1038/naturel l401 [epub]; re rs9939609 A, see e.g. Freathy RM, et al (2008). "Common variation in the FTO gene alters diabetes-related metabolic traits to the extent expected, given its effect on BMI". Diabetes 57 (5): 1419-26. doi: 10.2337/db07- 1466. PMC 3073395. PMID 18346983; re rsl421085 C, see e.g. Dina C, et al., (2007). "Variation in FTO contributes to childhood obesity and severe adult obesity". Nature Genetics 39 (6): 724-6. doi: 10.1038/ng2048. PMID 17496; and for multi-copy fto gene mouse, see e.g. Church et al., Overexpression of Fto leads to increased food intake and results in obesity, Nature Genetics, published online 14 Nov 2010, doi:10.1038/ng.713.
[033] In particular embodiments the person does not suffer from a pathogenic deficiency of L-DOPA (L-3,4-dihydroxyphenylalanine), does not indicate L-DOPA, and/or is not in need of L-DOPA (levopoda) or a dopaminergic agent, and/or the entacapone is not administered in conjunction with L-DOPA or a doaminergic agent.
[034] In particular embodiments, the method further comprises administering to the person an effective amount of one or more additional, different medicament for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly wherein the entacapone and medicament(s) are copackaged, coformulated or coadministered.
[035] In another aspect the invention provides pharmaceutical compositions comprising entacapone copackaged or coformulated with one or more of the additional, different medicaments for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, particularly different anti- weight gain medicaments. For example, the entacapone and additional, different anti- weight gain medicaments may be coformulated, particularly in unit dosage form, or unit dosage forms of each may be copackaged in a multipack adapted for sequential use, such as blisterpack, comprising sheets of unit dosage forms. Exemplary coformulation and copackagings are shown in Table 2.
[036] Table 2. Exemplary coformulation and copackagings. 1. Entacapone / Orlistat in 1000mg/120mg or 2000mg/120mg coformulated tablets.
2. Entacapone / Sibutramine in lOOOmg/lOmg or 2000mg/10mg coformulated tablets.
3. Entacapone / Lorcaserin in lOOOmg/lOmg or 2000mg/10mg coformulated tablets.
4. Entacapone / Rimonabant in 1000mg/20mg or 2000mg/20mg coformulated tablets.
5. Entacapone / Metformin in 1000mg/500mg or 2000mg/500mg coformulated tablets.
6. Entacapone / Exenatide: lOOOmg or 2000mg tablet / 250 mcg/mL solution (Byetta), copackaged
7. Entacapone / Exenatide: lOOOmg or 2000mg tablet / suspension powder, ER 2 mg (Bydureon), copackaged
8. Entacapone / Pramlintide: lOOOmg or 2000mg tablet / 600 mcg/mL (as acetate), copackaged
9. Entacapone / Phentermine in lOOOmg/lOmg or 2000mg/10mg coformulated tablets.
10. Entacapone / atorvastatin (Lipitor) in lOOOmg/lOmg coformulated tablets.
11. Entacapone / fluvastatin (Lescol) in 1000mg/80mg coformulated tablets.
12. Entacapone / lovastatin (Altoprev, Mevacor) in lOOOmg/lOmg coformulated tablets.
13. Entacapone / pravastatin (Pravachol) in lOOOmg/lOmg coformulated tablets.
14. Entacapone / rosuvastatin (Crestor) in 1000mg/5mg coformulated tablets.
15. Entacapone / simvastatin (Zocor) in lOOOmg/lOmg coformulated tablets.
16. Entacapone / cholestyramine (Prevalite, Questran) in 1000/5000mg copackaged powder.
17. Entacapone / colesevelam (Welchol) in 1000mg/625mg coformulated tablets.
18. Entacapone / colestipol (Colestid) in lOOOmg/lOOOmg coformulated tablets.
19. Entacapone / ezetimibe (Zetia) in lOOOmg/ lOmg coformulated tablets.
20. Entacapone / ezetimibe-simvastatin (Vytorin) in 1000/10/lOmg coformulated tablets.
21. Entacapone / fenofibrate (Lofibra, TriCor) in 1000mg/54mg coformulated tablets.
22. Entacapone / gemfibrozil (Lopid) in 1000mg/600mg coformulated tablets.
23. Entacapone / Niacin (Niaspan) in 1000mg/500mg coformulated tablets.
24. Entacapone / Omega-3 fatty acid (Lovaza) 1000mg/4mg coformulated tablets.
[037] By targeting different pathways the coadmininistered drugs can act supplementally or synergistically to increase the potency compared with separate administration, and thereby also permit use reduced or otherwise suboptimal or subtherapeutic dosages, if not coadministered, while maintaining efficacy, such as 50%/50% and 25%/25% coformulations and copackagings of those shown in Table 2. [038] The term "pharmaceutically acceptable salts" is meant to include salts of the active compounds which are prepared with relatively nontoxic acids or bases, depending on the particular substituents found on the compounds described herein. When compounds of the invention contain relatively acidic functionalities, base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent. Examples of pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amino, or magnesium salt, or a similar salt. When compounds of the invention contain relatively basic
functionalities, acid addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired acid, either neat or in a suitable inert solvent. Examples of pharmaceutically acceptable acid addition salts include those derived from inorganic acids like hydrochloric, hydrobromic, nitric, carbonic,
monohydrogencarbonic, phosphoric, monohydrogenphosphoric, dihydrogenphosphoric, sulfuric, monohydrogensulfuric, hydriodic, or phosphorous acids and the like, as well as the salts derived from relatively nontoxic organic acids like acetic, propionic, isobutyric, oxalic, maleic, malonic, benzoic, succinic, suberic, fumaric, mandelic, phthalic, benzenesulfonic, p- tolylsulfonic, citric, tartaric, methanesulfonic, and the like. Also included are salts of amino acids such as arginate and the like, and salts of organic acids like glucuronic or galactunoric acids and the like. Certain specific compounds of the invention contain both basic and acidic functionalities that allow the compounds to be converted into either base or acid addition salts.
[039] The neutral forms of the compounds may be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the invention.
[040] In addition to salt forms, the compounds may be in a prodrug form. Prodrugs of the compounds are those compounds that undergo chemical changes under physiological conditions to provide the compounds of the invention. Additionally, prodrugs can be converted to the compounds of the invention by chemical or biochemical methods in an ex vivo environment. For example, prodrugs can be slowly converted to the compounds of the invention when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent. Prodrugs are often useful because, in some situations, they may be easier to administer than the parent drug. They may, for instance, be more bioavailable by oral administration than the parent drug. The prodrug may also have improved solubility in pharmacological compositions over the parent drug. A wide variety of prodrug derivatives are known in the art, such as those that rely on hydrolytic cleavage or oxidative activation of the prodrug. An example, without limitation, of a prodrug would be a compound of the invention which is administered as an ester (the "prodrug"), but then is metabolically hydrolyzed to the carboxylic acid, the active entity.
[041] Subject compounds can exist in unsolvated forms as well as solvated forms, including hydrated forms. In general, the solvated forms are equivalent to unsolvated forms and are intended to be encompassed within the scope of the invention. Subject compounds may exist in multiple crystalline or amorphous forms. In general, all physical forms are equivalent for the uses contemplated and are intended to be within the scope of the invention.
[042] Certain subject compounds possess asymmetric carbon atoms (optical centers) or double bonds; the racemates, diastereomers, geometric isomers and individual isomers are all intended to be encompassed within the scope of the invention.
[043] The compositions for administration can take the form of bulk liquid solutions or suspensions, or bulk powders. More commonly, however, the compositions are presented in unit dosage forms to facilitate accurate dosing. The term "unit dosage forms" refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient. Typical unit dosage forms include prefilled, premeasured ampules or syringes of the liquid compositions or pills, tablets, capsules, losenges or the like in the case of solid compositions.
[044] A wide variety of suitable formulations and delivery systems, including suitable excipients or carriers and methods for preparing administrable compositions, are known or apparent to those skilled in the art and are described in more detail in such publications as Remington: The Science and Practice of Pharmacy (Pharmaceutical Press (2012). For example, in particular embodiments the compositions are formulated or delivered in extended or controlled delivery systems, such as diffusion systems (e.g. reservoir devices, matrix devices, diffusion-controlled implants and transdermal patches) and encapsulated and matrix dissolution systems, erosion products, osmotic pump systems, ion exchange resins, etc.
[045] In particular embodiment the amount administered is far in excess of, at least 2, 2.5, 5 or 10X that (200mg) currently indicated for Parkinson's Disease, and will preferably be 0.5-10, 0.5-5, 0.5-2.5, 1-10, 1-5, 1-2.5, 2-10, or 2-5g/day, in unit dosage forms of 0.25, 0.5, 1, 1.5, 2 or 2.5g. [046] The compounds can be administered by a variety of methods including, but not limited to, parenteral, topical, oral, or local administration, such as by aerosol or
transdermally, for prophylactic and/or therapeutic treatment. Also, in accordance with the knowledge of the skilled clinician, the therapeutic protocols (e.g., dosage amounts and times of administration) can be varied in view of the observed effects of the administered therapeutic agents on the patient, and in view of the observed responses of the disease to the administered therapeutic agents.
[047] Examples
[048] Structure-based Virtual Screening. We docked 1323 FDA- approved drugs to the substrate binding site in FTO, and filtered the docking poses based on physicochemical descriptors, such as the number of hydrogen bonds, buried carbon atoms and hydrophobic contact. Then, the docking poses were minimized and rescored using more sophisticated scoring method. One top-ranked drug is entacapone (Fig. 1), which is a COMT (Catechol-O- methyltransferase) inhibitor used for treating Parkinson disease.
[049] Enzymatic Inhibition. We measured the compound's inhibition activity in the demethylation reaction catalyzed by FTO. The assay was performed in 100 μΐ of reaction system containing 50 mM HEPES buffer (pH=7.0), 100 μΜ of a-KG, 100 μΜ of
(NH4)2Fe(S04)2, 1 mM of L-ascorbic acid, 50 μ^ιηΐ of BSA, 0.5 μΜ of ssDNA with N6-mA (5 ' -ATTGTCA(m6A)CAGCAGA-3 ' ), and 0.1 μΜ of FTO protein. The reaction system was incubated at 37 °C for 2 h and stopped by heating at 95 °C for 5 min. ssDNA was digested by nuclease PI and alkaline phosphatase. The concentrations of N6-mA and A were analyzed by HPLC-MS/MS. When concentration of substrate and enzyme are 0.5 μΜ and 0.1 μΜ, respectively, the measured IC50 value of entacapone against FTO is ~ 9 uM (Fig. 2).
[050] Cellular Triglyceride Synthesis Inhibition. We measured the compound's inhibition activity on triglyceride synthesis in Huh-7 cell line (Fig.3), the IC50 value of entacapone is ~ 15 uM.
[051] In vivo Anti-obesity Efficacy. Total of 23 male wistar rats (6 weeks) were fed with high-fat diet (45% fat, OpenSource Diets D12451), and entacapone (85.6 mg/day) was administered to 12 randomly selected rats by gavage. After 8 weeks, the mean body weight of drug treatment group was about 11% less than that of control group (*p-value < 0.05) (Fig. 4). However, the body-weight-normalized food intakes of the two groups showed no difference (Fig. 5). Interestingly, the LDL-c (Low Density Lipoprotein - cholesterol) in serum of drug treatment group decreased as about 40% comparing to that of control group (***p-value < 0.005) (Fig. 6 and Table 1). The adipose and hepatic tissues of rat in drug- treated group showed dramatic changes comparing to control group, with reduced size of adipose cells and reduced level of liver steatosis (Fig. 7 a,b H&E staining, 10 x
magnification; c,d H&E staining, 40x magnification).
[052] Table 1. Effects of Entacapone on serum biochemistry in rats. Difference is the percentage of concentration change of index in drug treatment group compared to that in control group.
Figure imgf000011_0001
[053] From these and other data we determined that anti-weight gain effective human dosages should be far in excess of the standard 200mg/day dosage for treating Parkinsons, preferably 0.5-10, 0.5-5, 0.5-2.5, 0.5-1, 1-10, 1-5, 1-2.5, 2-10, or 2-5g/day.
[054] Atherosclerosis Model: Ldlr-Deficient Mice. We measured entacapone' s anti- atherosclerosis efficacy using Ldlr~'~ mice fed western style diet (20% fat, 0.15% cholesterol), entacapone (300 mg/day) was orally administered by blending with diet. After 8 weeks, the mean lesion area in aortic sinuses of drug treatment group was 28% less than that of control group (p-value=0.08) (Fig. 8). Therefore, entacapone reduces atherosclerosis symptom, comparing with the control.
[055] In vivo Anti-obesity Efficacy in obese mice. Male C57BL/6 mice were fed with high-fat diet (45% fat, OpenSource Diets D12451) for 8 weeks. Then obese mice with body weight 20% larger than that of mice fed with normal diet (20 mice) were selected for experiments. Entacapone (300 mg/kg) was orally administered to 10 randomly selected obese mice by blending with diet. After 13 weeks, the mean body weight gain of drug treatment group was about 11% less than that of control group (*p-value < 0.05) (Fig. 9). [056] References
1. Frayling, T. M. et al. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity. Science 316, 889-894, (2007).
2. Scuteri, A. et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PLoS Genet 3, el 15, doi:07-PLGE-RA-0253 (2007).
3. Scott, L. J. et al. A genome- wide association study of type 2 diabetes in Finns detects multiple susceptibility variants. Science 316, 1341-1345, (2007).
4. Dina, C. et al. Variation in FTO contributes to childhood obesity and severe adult obesity. Nat Genet 39, 724-726, (2007).
5. Gao, X. et al. The fat mass and obesity associated gene FTO functions in the brain to regulate postnatal growth in mice. PLoS One 5, el4005, (2010).
6. Fischer, J. et al. Inactivation of the Fto gene protects from obesity. Nature 458, 894- 898, (2009).
7. Church, C. et al. A mouse model for the metabolic effects of the human fat mass and obesity associated FTO gene. PLoS Genet 5, el000599, (2009).
8. Church, C. et al. Overexpression of Fto leads to increased food intake and results in obesity. Nat Genet 42, 1086-1092, (2010).
9. Gerken, T. et al. The obesity-associated FTO gene encodes a 2-oxoglutarate- dependent nucleic acid demethylase. Science 318, 1469-1472 (2007).
10. Han, Z. et al. Crystal structure of the FTO protein reveals basis for its substrate specificity. Nature 464, 1205-1209, (2010).
11. Meyer, K. D. et al. Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3' UTRs and near Stop Codons. Cell 149, 1635-1646 (2012).
12. Jia, G. et al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity- associated FTO. Nat Chem Biol 7, 885-887, (2011).
It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein, including citations therein, are hereby incorporated by reference in their entirety for all purposes.

Claims

WHAT IS CLAIMED IS:
1. A method of inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating an obesity related disease or ameliorating or reducing the pathology or severity of an obesity related disease or a symptom of an obesity related disease, comprising administering to a person in need thereof an effective amount of entacapone ((2E)-2-cyano-3-(3,4-dihydroxy-5-nitrophenyl)-N,N-diethylprop-2-enamide), or a pharmaceutically-acceptable salt thereof.
2. The method of claim 1 wherein the obesity related disease is selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, and liver, kidney or thyroid diseases.
3. The method of claim 1 wherein the amount is 0.5-5 g/day.
4. The method of claim 1 wherein the person meets one or more criteria:
(a) is not diagnosed with, or is not symptomatic of Parkinson's disease;
(b) is less than 50 years old;
(c) is obese or over-weight;
(d) suffers from or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases;
(e) has genotype: SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A), or a surrogate or proxy SNP in linkage disequilibrium therewith and having a r value greater than 0.5; or
(f) pathogenically expresses or over-expresses FTO or Fto.
5. The method of claim 1 further comprising detecting in the person (a) genotype SNP rs7202116 (G), or (b) over-expression of FTO or Fto.
6. The method of claim 1 further comprising administering to the person an effective amount of a second, different medicament for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
7. A method of inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, comprising administering to a person in need thereof an effective amount of entacapone, or a pharmaceutically-acceptable salt thereof, in conjunction with a second, different medicament for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
8. The method of claim 7 wherein the amount is 0.5-5 g/day.
9. The method of claim 7 wherein the entacapone and medicament are copackaged, coformulated or coadministered.
10. The method of claim 7 wherein the person meets one or more criteria:
(a) is not diagnosed with, or is not symptomatic of Parkinson's disease;
(b) is less than 50 years old;
(c) is obese or over-weight;
(d) suffers from or is diagnosed with an obesity related disease selected from diabetes, hyperglycemia, diabetic nephropathy, hyperlipemia, coronary heart disease, atherosclerosis, hypertension, cardiovascular or cerebrovascular diseases, or liver, kidney or thyroid diseases;
(e) has genotype: SNP rs7202116 (G), rsl421085 (C), or rs9939609 (A), or a surrogate or proxy SNP in linkage disequilibrium therewith and having a r2 value greater than 0.5; or
(f) pathogenically expresses or over-expresses FTO or Fto.
11. The method of claim 7 further comprising detecting in the person genotype SNP rs7202116 (G), or over-expression of FTO or Fto.
12. A composition comprising entacapone, or a pharmaceutically-acceptable salt thereof, copackaged or coformulated with a second, different medicament for inhibiting weight gain, promoting weight loss, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis.
13. The composition of claim 12 wherein the entacapone is present in a unit dosage of 0.5-5 g-
14. The composition of claim 12 wherein the medicament is a medicament for inhibiting weight gain that is a food intake inhibitor or a food absorption inhibitor.
15. The composition of claim 12 wherein the medicament is a medicament for inhibiting weight gain that is Orlistat, Sibutramine, Lorcaserin, Rimonabant, Metformin , Exenatide , Pramlintide, phentermine/topiramate, or a pharmaceutically- acceptable salt thereof.
16. The composition of claim 12 wherein the medicament is a medicament for reducing serum LDL, cholesterol, LDL-c, or triglycerides, that is atorvastatin (Lipitor), fluvastatin (Lescol), lovastatin (Altoprev, Mevacor), pravastatin (Pravachol), rosuvastatin (Crestor), simvastatin (Zocor), cholestyramine (Prevalite, Questran), colesevelam (Welchol), colestipol (Colestid), ezetimibe (Zetia), ezetimibe-simvastatin (Vytorin), fenofibrate (Lofibra, TriCor), gemfibrozil (Lopid), Niacin (Niaspan), Omega-3 fatty acid (Lovaza), or a pharmaceutically- acceptable salt thereof.
17. A composition for inhibiting weight gain, reducing serum LDL, cholesterol, LDL-c, or triglycerides, or treating atherosclerosis, comprising a unit dosage form of 0.5-2.5 g entacapone, or a pharmaceutically-acceptable salt thereof.
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