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WO2008118943A1 - Electrospun apatite/polymer nano-composite scaffolds - Google Patents

Electrospun apatite/polymer nano-composite scaffolds Download PDF

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Publication number
WO2008118943A1
WO2008118943A1 PCT/US2008/058234 US2008058234W WO2008118943A1 WO 2008118943 A1 WO2008118943 A1 WO 2008118943A1 US 2008058234 W US2008058234 W US 2008058234W WO 2008118943 A1 WO2008118943 A1 WO 2008118943A1
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WO
WIPO (PCT)
Prior art keywords
particles
fibers
plla
approximately
scaffold
Prior art date
Application number
PCT/US2008/058234
Other languages
French (fr)
Inventor
Mei Wei
Fei Peng
Zhi-Kang Xu
Original Assignee
The University Of Connecticut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Connecticut filed Critical The University Of Connecticut
Priority to EP08732834A priority Critical patent/EP2129517A4/en
Priority to CN200880008069A priority patent/CN101687384A/en
Priority to CA002680586A priority patent/CA2680586A1/en
Priority to JP2010501183A priority patent/JP2010522620A/en
Publication of WO2008118943A1 publication Critical patent/WO2008118943A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/48—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with macromolecular fillers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24058—Structurally defined web or sheet [e.g., overall dimension, etc.] including grain, strips, or filamentary elements in respective layers or components in angular relation
    • Y10T428/24124—Fibers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2922—Nonlinear [e.g., crimped, coiled, etc.]
    • Y10T428/2924—Composite
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2927—Rod, strand, filament or fiber including structurally defined particulate matter

Definitions

  • the present invention relates generally to composite materials.
  • the present invention also relates generally to methods of making composite materials.
  • natural bone is a composite material that includes hydroxyapatite (HA) and fibrous collagen.
  • HA hydroxyapatite
  • fibrous collagen In natural bone, the HA crystals are embedded within the collagen fiber matrix and are aligned along the long axis of fibers.
  • an apatite/fibrous polymer nano-composite scaffold has been fabricated using electrospinning.
  • Electrospinning is a convenient and versatile fabrication technique which produces fibers with diameters from approximately 50 nm to several micrometers.
  • the structure generated by electrospinning is highly porous with interconnected pores. This fibrous structure typically resembles the architecture of an extracellular matrix (ECM).
  • ECM extracellular matrix
  • These fibrous structures may be used as artificial bone composite.
  • these fibrous structures may be used with other tissues based on biocompatibility, mechanical properties, and cell attachment and growth of the fibrous structures and the tissues.
  • HA particles with sizes ranging from approximately 10 nm to approximately 10 ⁇ m and having an average aspect ratio up to approximately 50 are synthesized.
  • the HA particles are well dispersed in the spinning dope and co-elcctrospun with polymer nanofibcrs.
  • the HA/PLLA nano-compositc fibrous scaffold can be fabricated with HA particles homogenously distributed within the PLLA nano fibers.
  • up to approximately 20 wt% of HA nanoparticles is incorporated into the PLLA nanofibers. These nanoparticles are well aligned along the long axes of the polymer fibers.
  • Such obtained microstructure closely mimics the micro- arrangement of the inorganic/organic components in the ECM of natural bone.
  • Such fabricated scaffolds have desirable mechanical properties and good cell signaling properties. Al least in view of the above, such scaffolds are suitable for loading cells and biological active agents. It should also be noted that incorporation of more than 20 wt% HA nanoparticles is also within the scope of certain embodiments of the present invention.
  • fOOlGj It is desirable to fabricate bone graft materials mimicking the structural, mechanical, and biological behavior of natural bone. This need is met, to a great extent, by certain embodiments of the present invention, particularly those wherein a structure is provided that includes a scaffold and highly crystallized, well-dispersed HA nanoparticles. In this structure, the HA nanoparticles have controllable aspect ratios within the range of approximately 5 and approximately 50.
  • a structure that includes a fibrous matrix that itself includes a plurality of fibers.
  • the structure also includes a plurality of hydroxyapatite (HA) particles dispersed within the fibrous matrix, wherein the HA particles are substantially aligned along long axes of the plurality of fibers.
  • HA hydroxyapatite
  • a method of forming a structure includes adding hydroxyapatile (HA) particles to a poly-(L-lactic acid) (PLLA) solution to form a mixture and forming an HA/PLLA fiber by clectrospinning the mixture.
  • HA hydroxyapatile
  • PLLA poly-(L-lactic acid)
  • a structure that includes a fibrous matrix including a plurality of fibers.
  • the structure also includes a plurality of hydroxyapatite (HA) particles dispersed within the fibrous matrix, wherein the HA particles are substantially aligned along long axes of the plurality of fibers, wherein the structure is manufactured by adding the HA particles to a poly-(L-lactic acid) (PLLA) solution to form a mixture and by forming HA/PLLA fibers by electrospinning the mixture to form the fibrous matrix.
  • HA hydroxyapatite
  • PLLA poly-(L-lactic acid)
  • FIGS. l(a)-(c) illustrate morphologies of electrospun PLLA
  • FIG. l(a) illustrates a field emission scanning electron microscope (FESEM) image of electrospun PLLA nanofibers
  • FIG. l(b) illustrates an FESEM image of HA/PLL ⁇ (20: 80 w/w) composite nanofibers
  • FlG. l(c) illustrates a transmission electron microscope (TEM) image of HA/PLLA/HA (20: 80 w/w) composite nanofibers.
  • FESEM field emission scanning electron microscope
  • FIGS. 2(a)-(f) illustrate the effect of varying various electrospinning parameters.
  • FIG. 2(a) illustrates the effect of varying PLLA concentration in the electrospinning dope.
  • FIG. 2(b) illustrates the effect of varying the amount of HA incorporation (wt%) in the composite fibers.
  • FIG.2(c) illustrates the effect of varying power voltage.
  • FIG. 2(d) illustrates the effect of varying the injection rate.
  • FIG. 2(e) illustrates the effect of varying the spinneret inner diameter.
  • FIG.2(f) illustrates the effect of varying the distance between the spinneret tip and the collector on the diameter of the electrospun nanofibers.
  • FIGS.3(a)-(c) illustrate functionalized PLLA fibers.
  • FIG.3(a) is a TEM image of polyethylene glycol (PEG)-core-PLLA-sbell nanofibers.
  • FIG.3(b) is an FESEM image of highly aligned PLLA ⁇ ano fibers.
  • FIG. 3(c) is an FESEM image of highly porous PLLA nano fibers.
  • FIGS.4(a)-(c) illustrate the mechanical properties of electrospun PLLA and HA/PLLA scaffolds.
  • FlG.4(a) illustrates typical stress v. strain curves for HA/PLLA electrospun scafolds with an averaged fiber diameters equal to 110 ⁇ 15 nm.
  • FIG. 4(b) illustrates Young's moduli (E, hatched bars) and tensile stresses (solid bars) of HA/PLLA elecirospun scaffolds with averaged fiber diameters equal to 110 ⁇ 15 nm.
  • FIG. 4(c) illustrates Young ' s moduli (E) and tensile stresses of different component HA/PLLA electrospun scaffolds with averaged fiber diameters equal to 170 ⁇ 25 nm.
  • FIGS 5(a) and (b) illustrate the mechanical properties of electrospun fibrous scaffolds with different compositions and fibrous assemblies.
  • FIG. 5(a) illustrates the stress vs. strain curves for electrospun fibrous scaffolds with different compositions and fibrous assemblies.
  • FlG. 5(b) illustrates the tensile test results for electrospun fibrous scaffolds with different compositions and fibrous assemblies.
  • FIGS. 6(a)-(e) illustrate PLLA nanofibers obtained from clectrospinning according to certain embodiments of the present invention.
  • FIG. ⁇ (a) illustrates the average diameters and standard variations for Fibers electrospun from different PLLA-concentrations.
  • FIG. 6(b)-(c) were all taken at a magnification of *20,000.
  • FIGS. 7(a)-(d) illustrate electrospun HA/PLLA composites.
  • the IiA particles in the composite were synthcsizedvia a metathesis reaction at 100 0 C in FIG. 6(a), via a metathesis reaction at 7O 0 C in FIG. 7(b), via a metathesis reaction at 95 0 C in FIG. 7(c), and via a urea decomposition at 95 0 C in FIG. 7(d).
  • HA particles with different sizes and aspect ratios are evenly distributed within the illustrated polymer nanofibers.
  • the composites illustrated in FIGS.7(a)-(d) demonstrate a good orientation along the long axis of the PLA nanofibers. The HA content in these composites arc 20 wt%.
  • FIGS. 8(a)-(b) illustrate thin layers of a biomimetic apatite coating on the surface of PLLA and HA/PLLA scaffolds, respectively.
  • FIG. 9 illustrates in vitro release of FITC-BSA from electrospun HA/PLLA fibrous scaffold with needle-shape HA particles either at nano- (NHA) or microsize (MHA) and with either random or aligned fibrous assembly.
  • FIG. 10 illustrates relative cell viability on different scaffolds after being cultured for 3, 7, and 10 days.
  • Sample A PLLA scaffold with random assembly
  • Sample B micrometer-size HA/PLLA scaffold with random assembly
  • Sample C nanometer-size HA/PLLA scaffold with random assembly
  • sample D biomimetic apatite-coated nanometer-size HA/PLLA scaffold with random assembly
  • sample E micrometer-size HA/PLLA scaffold with aligned assembly
  • sample F nanometer-size HA/PLLA scaffold with aligned assembly.
  • FIG. 1 1 illustrates relative alkaline phosphatase (ALP) activities on different scaffolds after being cultured for 7 and 10 days.
  • ALP alkaline phosphatase
  • carbonated HA, and other calcium phosphates e.g., ion-substituted apatites, such as carbonate hydroxyapatite, fJuorinatcd hydroxyapatitc, chlorinated hydroxyapatite, silicon-containing hydroxyapatite, magnesium-containing hydroxyapatite and other ion substituted HA, tricalcium phosphate, tctracalcium phosphate, monetite, dicalcium phosphate, dicalcium phosphate dihydrate, octacalcium phosphate, or calcium sulfate) may also be used.
  • ion-substituted apatites such as carbonate hydroxyapatite, fJuorinatcd hydroxyapatitc, chlorinated hydroxyapatite, silicon-containing hydroxyapatite, magnesium-containing hydroxyapatite and other ion substituted HA, tricalcium phosphate, tctracalc
  • HA particles were added to a PLLA solution to fabricate an HA/PLLA composite. Also, the amount of HA in the PLLA solution was adjusted by varying the HA to PLLA feeding ratio in the spin-dope.
  • HA up to approximately 20 wt% of HA is incorporated into PLLA nanofibcrs.
  • These HA particles are typically well aligned along the long axis of the polymer fibers.
  • the size of the HA particles have an average width of at least 10 nm and an average length ranged from approximately 10 nm to approximately 10 ⁇ m. with an average aspect ratio up to approximately 50.
  • the particles, according to certain embodiments of the present invention were homogcnously distributed within the PLLA nanofibers after elcctrospinning. The resultant microstr ⁇ cture closely mimicked the arrangement of the inorganic/organic components in ECM of natural bone.
  • the HA/PLLA scaffold has improved mechanical properties and biocompatibility.
  • HA particles with lengths between 100 and 200 nm and aspect ratios between 7 and 10 were evenly distributed within HA/PLLA fiber bodies. These particles also demonstrated a good orientation along the long axes of the PLLA nanofibers.
  • FIG. 2(a) The effects of altering the electrospinning processing parameters on the diameter of HA/PLLA composite fibers were studied in FIG. 2. As illustrated, varying the polymer concentration of the electrospinning dope and varying the HA/PLLA weight ratio have the most obvious influences on the diameter. As illustrated in FIG. 2(a), the diameter of composite nanofibers increase with the PLLA concentration in the spinning dope, which indicates that a high PLLA concentration dope has higher surface tension and was more di ITicult to be spun into finer fibers during the fiber spinning process. FIG. 2(b) illustrates that composite fiber diameter decreases with increasing HA weight ratio. This is likely explained by the fact that higher amounts of HA in a composite decreases the viscosity of the electrospinning dope and the surface tension thereof as well.
  • FIG. 3 As illustrated in FIG. 3, several modified electrospinning techniques for fabricating functionalized nanofibrous scaffolds have been implemented according to various embodiments of the present invention.
  • a PEG/PLLA core-shell structure has been co-elcctrospun into a fibrous composite scaffold using co-axial dual spinnerets, as shown in FIG. 3(a).
  • Second, highly aligned nanofibers were fabricated using a rotating drum as the collector, as illustrated in FIG. 3(b). Scaffolds prepared in this manner have good orientation and improved mechanical strength along the long axes of the fibers.
  • DCM CH 2 Cl 2
  • DMF DMF
  • the porosity and pore size of fibers according to certain embodiments of the present invention can be adjusted by altering the solvent used and dope concentration.
  • the porous surface of the fibers can be used, for example, for controlled deliver)' of growth factors.
  • the porous surface will also enhance the bonding strength between the polymer fiber and a biomimetic apatite coating, such as the one illustrated in FIG. 8(b).
  • the porous surface may also act as a nucleation site for apatite further growth.
  • FIG. 5(a)-(b) illustrates the mechanical properties of electrospun PLLA-based scaffolds using tensile test.
  • One of skill in the art will recognize that both the alignment of the scaffold assembly and the incorporation of nano-sizc. needle-shape HA particles into the nano-fibers significantly improved the clastic modulus of the composite scaffold.
  • the scaffold with HA particles are much stiffer than those without HA particles, and the elastic modulus of the former is more than two times as high as that of the later. It is also shown that, the HA nanoparticles inhibit un-folding and orientation of PLLA molecular chains within spun fibers during tensile testing, i.e. cold drawing of the scaffold, and decrease the toughness of the composite scaffolds by decreasing their elongation at break.
  • the elastic modulus of the scaffolds with aligned assembly is four to five times higher than those with random fibrous assembly.
  • the pure PLLA scaffold with aligned assembly has much higher toughness but lower elongation at break than those with a random assembly. In the case of HA/PLLA scaffolds, such difference is not as significant as that of the pure PLLA scaffolds.
  • a homogenous apatite coating layer can also be formed on the surface of both PLLA and HA/PLLA scaffolds, as shown in FIGS. 8(a)-(b).
  • the thickness of the coating is a few micrometers, which was obtained after approximately 4 hr of immersion in a modified simulated body fluid (m-SBF).
  • the thickness of the coating can be adjusted by varying the Ca and P ion concentrations in SBF, sample immersion time, and pH of the solution.
  • maintaining the immersion time short is important in order to maintain the integrity of the polymer fibers.
  • some polymer fibers absorb water, which leads to the reduction of their mechanical properties.
  • the thickness of the coating can be adjusted by varying the coating conditions such as, the m-SBF pH, immersion time, and calcium and phosphorous concentrations.
  • fluorescein isothiocya ⁇ atc labeled bovine serum albumin (F1TC-BS.A) was incorporated into the biomimetic apatite coating formed-on the surfaces of the scaffold, to study the drug release behaviors of the electrospun scaffolds.
  • the drug release profiles of the electrospun HA/PLLA fibrous scaffolds are shown in FlG 9.
  • the release of FITC-BSA from the biomimetic coating on the scaffold was studied for a time period of 8 weeks. Sustained release profiles have been observed for all scaffolds.
  • the scaffolds with nano-si7.e HA particles (NHA) showed faster release profiles than those incorporated with micro-size HA particles (MHA).
  • the biomimetic coating formed on the electrospun scaffold can be an effective carrier for sustained release of proteins and/or drugs.
  • FIG. 10 illustrates relative cell viability on different scaffolds.
  • more cells were attached to the surface of the scaffold.
  • more cells were found on the scaffolds incorporated with either nano- or micro-size HA particles than those on pure PLLA scaffold after 7 days of culture. After 10 days, such difference became much more significant.
  • the cell alkaline phosphatase (ALP) activities, an early marker of bone formation, on different scaffolds are shown in Figure 11.
  • ALP alkaline phosphatase
  • a thicker apatite coating was obtained for the HA/PLLA scaffolds than the pure PLL ⁇ scaffolds with the same SBF soaking time. This may be explained by the fact that some of the HA particles loaded in the PLLA fibers position themselves on the surfaces of the fibers and act as nucleation sites for the apatite coating growth. Also, according to certain embodiments of the present invention, the coating grew more effectively on the top surface than the interior for both pure PLLA and HA/PLLA scaffold.
  • HA/PLLA composite fibrous scaffolds that include micro-scale pores throughout the body of the scaffold owing to electrospinning are also within the scope of the present invention.
  • Such scaffolds include nanometer-size pores on the surface of fibers in the scaffold owing to an evaporation process of highly volatile solvent.
  • nanoporous surfaces on composite fibers in the scaffold not only contribute to better bonding between a fiber substrate and an HA coating applied through a biomimetic coating method, but also induce fast degradation of the composite fibers.
  • a pumping device in order to promote a more homogenous apatite coating throughout the scaffold, a pumping device is used to assist ra-SBF penetrating into the scaffold or to create relatively large pores in the scaffold. Pores in the range of hundreds of micrometers, according to certain embodiments of the present invention, arc desirable for both the invasion of blood vessels to provide the necessary nutrient
  • HA/PLLA composite fibrous scaffolds that include at least one composite fiber surface and an HA coating on the composite fiber surface.
  • the coating is formed by using a biomimetic coating method.
  • the obtained HA coating layer on the fiber surface will typically not only increase the HA component within the scaffold and contribute to improved mechanical properties of the scaffold, but will also increase the exposure of HA to the surrounding tissue during in vivo application. Such exposure can improve the biocompatibility as well as the osteoconductivity of die composite scaffold.
  • a HA/PLLA composite fibrous scaffold that includes poly-lactic-co- glycolic acid (PLGA) microspheres incorporated among fibers.
  • PLGA poly-lactic-co- glycolic acid
  • the size of the microspheres is controlled to be above 100 micrometers. This typically not only increases the mechanical properties of the fibrous scaffold but may also be used as a carrier for releasing one or more different drugs.
  • a method of multi-drug delivery may be implemented. For example, two or more different drugs may be preloaded into different components of an elcctrospinning composite dope and PLGA microspheres to form a composite fibrous scaffold Then, the drugs may be subsequently controllably released.

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Abstract

An artificial bone composite structure is provided. This structure includes a fibrous matrix that itself includes a plurality of fibers. Also, the structure includes a plurality of hydroxyapatite (HA) particles. These particles are dispersed within the fibrous matrix. Also, the HA particles have controlled size and aspect ratios and are aligned along long axes of the fibers. In some instances, the fibers include poly-(L-lactic acid) (PLLA).

Description

ELECTROSPUN APATITE/POLYMER NANO-COMPOSITE SCAFFOLDS
CROSS-REFERENCE TO RELATED APPLICATIONS |OOϋl] This application claims priority to provisional U.S. patent application entitled, "Electrospun Apatite/Polymer Nano-Composite Scaffolds," filed March 26, 2007, having serial number 60/907,207, now pending, the disclosure of which is hereby incorporated by reference in its entirety.
FIELD OF THE INVENTION
[0002] The present invention relates generally to composite materials. The present invention also relates generally to methods of making composite materials.
BACKGROUND OF TOE INVENTION
|0003] One prominent area of current scientific research in the medical field is focused upon artificially replicating human bones and other types of tissues. One of the goals of such research is to provide surgeons with artificially fabricated materials that may then be incorporated into a human patient during surgery.
[00041 Currently, some surgeons remove bone or tissues from one portion of a patient's body and reattach the bone or tissues in another portion of the patient's body. For example, during spinal surgery, bone from the hip is sometimes removed and incorporated into the spine. Some other surgeons are forced to incorporate metal components (e.g., metal rods and/or plates) in portions of a patient's body where natural bone has been shattered or has deteriorated.
[0005] Structurally, natural bone is a composite material that includes hydroxyapatite (HA) and fibrous collagen. In natural bone, the HA crystals are embedded within the collagen fiber matrix and are aligned along the long axis of fibers.
[0006| Currently, no method exists for artificially replicating the exact structure of natural bone. Even the most advanced methods for artificially replicate natural bone structure have at least been unsuccessful in aligning HA crystals in a manner analogous to the alignment in natural bone. As such, artificially generated bone does not have the same mechanical/biological/chemical properties as naturally occurring bone.
SUMMARY OF THE INVENTION
[0007] According to certain embodiments of the present invention, an apatite/fibrous polymer nano-composite scaffold has been fabricated using electrospinning. Electrospinning is a convenient and versatile fabrication technique which produces fibers with diameters from approximately 50 nm to several micrometers. According to certain embodiments of the present invention, the structure generated by electrospinning is highly porous with interconnected pores. This fibrous structure typically resembles the architecture of an extracellular matrix (ECM). These fibrous structures may be used as artificial bone composite. Furthermore, these fibrous structures may be used with other tissues based on biocompatibility, mechanical properties, and cell attachment and growth of the fibrous structures and the tissues.
(0008J According to certain other embodiments of the present invention, HA particles with sizes ranging from approximately 10 nm to approximately 10 μm and having an average aspect ratio up to approximately 50 are synthesized. The HA particles are well dispersed in the spinning dope and co-elcctrospun with polymer nanofibcrs. The HA/PLLA nano-compositc fibrous scaffold can be fabricated with HA particles homogenously distributed within the PLLA nano fibers. [0009] According to still other embodiments of the present invention, up to approximately 20 wt% of HA nanoparticles is incorporated into the PLLA nanofibers. These nanoparticles are well aligned along the long axes of the polymer fibers. Such obtained microstructure closely mimics the micro- arrangement of the inorganic/organic components in the ECM of natural bone. Such fabricated scaffolds have desirable mechanical properties and good cell signaling properties. Al least in view of the above, such scaffolds are suitable for loading cells and biological active agents. It should also be noted that incorporation of more than 20 wt% HA nanoparticles is also within the scope of certain embodiments of the present invention. fOOlGj It is desirable to fabricate bone graft materials mimicking the structural, mechanical, and biological behavior of natural bone. This need is met, to a great extent, by certain embodiments of the present invention, particularly those wherein a structure is provided that includes a scaffold and highly crystallized, well-dispersed HA nanoparticles. In this structure, the HA nanoparticles have controllable aspect ratios within the range of approximately 5 and approximately 50.
[0011] According to other embodiments of the present invention, a structure is provided that includes a fibrous matrix that itself includes a plurality of fibers. The structure also includes a plurality of hydroxyapatite (HA) particles dispersed within the fibrous matrix, wherein the HA particles are substantially aligned along long axes of the plurality of fibers.
[0012] According to yet other embodiments of the present invention, a method of forming a structure is provided. The method includes adding hydroxyapatile (HA) particles to a poly-(L-lactic acid) (PLLA) solution to form a mixture and forming an HA/PLLA fiber by clectrospinning the mixture.
[0013J According to still other embodiments of the present invention, a structure is provided that includes a fibrous matrix including a plurality of fibers.
The structure also includes a plurality of hydroxyapatite (HA) particles dispersed within the fibrous matrix, wherein the HA particles are substantially aligned along long axes of the plurality of fibers, wherein the structure is manufactured by adding the HA particles to a poly-(L-lactic acid) (PLLA) solution to form a mixture and by forming HA/PLLA fibers by electrospinning the mixture to form the fibrous matrix.
[0014] There has thus been outlined, rather broadly, certain embodiments of the invention in order that the detailed description thereof may be better understood, and in order that the present contribution to the art may be better appreciated, There are, of course, additional embodiments of the invention that will be described below and which will form the subject matter of the claims appended hereto.
J0015] In this respect, before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and to the arrangements of the components set forth in the following description or1 illustrated in the drawings. 'ITie invention is capable of embodiments in addition to those described and of being practiced and carried out in various ways. Also, it is to be understood that lhe phraseology and terminology employed herein, as well as the abstract, are for the purpose of description and should not be regarded as limiting.
(0016] As such, those skilled in the art will appreciate that the conception upon which this disclosure is based may readily be utilized as a basis for the designing of other structures, methods and systems for carrying out the several purposes of the present invention. It is important, therefore, that the claims be regarded as including such equivalent constructions insofar as they do not depart from the spirit and scope of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS [0017] FIGS. l(a)-(c) illustrate morphologies of electrospun PLLA and
HA/PLLA composite nanofibers. More specifically, FIG. l(a) illustrates a field emission scanning electron microscope (FESEM) image of electrospun PLLA nanofibers, FlG. l(b) illustrates an FESEM image of HA/PLLΛ (20: 80 w/w) composite nanofibers, and FlG. l(c) illustrates a transmission electron microscope (TEM) image of HA/PLLA/HA (20: 80 w/w) composite nanofibers.
[0018] FIGS. 2(a)-(f) illustrate the effect of varying various electrospinning parameters. FIG. 2(a) illustrates the effect of varying PLLA concentration in the electrospinning dope. FIG. 2(b) illustrates the effect of varying the amount of HA incorporation (wt%) in the composite fibers. FIG.2(c) illustrates the effect of varying power voltage. FIG. 2(d) illustrates the effect of varying the injection rate. FIG. 2(e) illustrates the effect of varying the spinneret inner diameter. FIG.2(f) illustrates the effect of varying the distance between the spinneret tip and the collector on the diameter of the electrospun nanofibers.
(0019) FIGS.3(a)-(c) illustrate functionalized PLLA fibers. FIG.3(a) is a TEM image of polyethylene glycol (PEG)-core-PLLA-sbell nanofibers. FIG.3(b) is an FESEM image of highly aligned PLLA πano fibers. FIG. 3(c) is an FESEM image of highly porous PLLA nano fibers.
[0020] FIGS.4(a)-(c) illustrate the mechanical properties of electrospun PLLA and HA/PLLA scaffolds. FlG.4(a) illustrates typical stress v. strain curves for HA/PLLA electrospun scafolds with an averaged fiber diameters equal to 110 ± 15 nm. FIG. 4(b) illustrates Young's moduli (E, hatched bars) and tensile stresses (solid bars) of HA/PLLA elecirospun scaffolds with averaged fiber diameters equal to 110 ± 15 nm. FIG. 4(c) illustrates Young's moduli (E) and tensile stresses of different component HA/PLLA electrospun scaffolds with averaged fiber diameters equal to 170 ± 25 nm.
[0021] FIGS 5(a) and (b) illustrate the mechanical properties of electrospun fibrous scaffolds with different compositions and fibrous assemblies.
FIG. 5(a) illustrates the stress vs. strain curves for electrospun fibrous scaffolds with different compositions and fibrous assemblies. FlG. 5(b) illustrates the tensile test results for electrospun fibrous scaffolds with different compositions and fibrous assemblies.
[0022] FIGS. 6(a)-(e) illustrate PLLA nanofibers obtained from clectrospinning according to certain embodiments of the present invention. FIG. β(a) illustrates the average diameters and standard variations for Fibers electrospun from different PLLA-concentrations. The concentration used in FlG. 6(b) was PLLA=4.0 wt%. The concentration used in FIG. 6(c) was PLLA=6.0 wt%. The concentration used in FIG. 6(d) was PLLA=8.0 wt%. The concentration used in FIG. 6(e) was PLLA=I 0.0wt%. FIG. 6(b)-(c) were all taken at a magnification of *20,000.
[0023] FIGS. 7(a)-(d) illustrate electrospun HA/PLLA composites. The IiA particles in the composite were synthcsizedvia a metathesis reaction at 1000C in FIG. 6(a), via a metathesis reaction at 7O0C in FIG. 7(b), via a metathesis reaction at 950C in FIG. 7(c), and via a urea decomposition at 950C in FIG. 7(d). One of skill in the art will recognize that HA particles with different sizes and aspect ratios are evenly distributed within the illustrated polymer nanofibers. Also, the composites illustrated in FIGS.7(a)-(d) demonstrate a good orientation along the long axis of the PLA nanofibers. The HA content in these composites arc 20 wt%.
(0024) FIGS. 8(a)-(b) illustrate thin layers of a biomimetic apatite coating on the surface of PLLA and HA/PLLA scaffolds, respectively.
[0025) FIG. 9 illustrates in vitro release of FITC-BSA from electrospun HA/PLLA fibrous scaffold with needle-shape HA particles either at nano- (NHA) or microsize (MHA) and with either random or aligned fibrous assembly.
[0026] FIG. 10 illustrates relative cell viability on different scaffolds after being cultured for 3, 7, and 10 days. (Sample A) PLLA scaffold with random assembly, (Sample B) micrometer-size HA/PLLA scaffold with random assembly. (Sample C) nanometer-size HA/PLLA scaffold with random assembly,
(Sample D) biomimetic apatite-coated nanometer-size HA/PLLA scaffold with random assembly, (Sample E) micrometer-size HA/PLLA scaffold with aligned assembly, and (Sample F) nanometer-size HA/PLLA scaffold with aligned assembly.
[0027] FIG. 1 1 illustrates relative alkaline phosphatase (ALP) activities on different scaffolds after being cultured for 7 and 10 days. (Sample A) PLLA scaffold with random assembly, (Sample B) micrometer-size HA/PLLA scaffold with random assembly, (Sample C) nanometer-size HA/PLLA scaffold with random assembly, (Sample D) biomimetic apatite-coated nanometer-size HA/PLLA scaffold with random assembly, (Sample E) micrometer-size HA/PLLA scaffold with aligned assembly, and (Sample F) nanometcr-sizc HA/PLLA scaffold with aligned assembly.
DETAILED DESCRIPTION
(0028] The invention will now be described with reference to the drawing figures, in which like reference numerals refer to like parts throughout. According to certain embodiments of the present invention, HA/PLLA composite scaffolds are electrospun. However, it should be noted that other composite systems using materials other than HA and PLLA are also within the scope of certain embodiments of the present invention. For example, collagen, hyaluronans, fibrin, chitosan, alginate, other animal- or plant-derived polymers. PLA, PCL, PGA3 other synthetic and natural polymers, polyesters, polyethcrs, polycarbonates, polyamines, polyamides, and their co-polymcrs and combinations may be used. Also, for example, carbonated HA, and other calcium phosphates (e.g., ion-substituted apatites, such as carbonate hydroxyapatite, fJuorinatcd hydroxyapatitc, chlorinated hydroxyapatite, silicon-containing hydroxyapatite, magnesium-containing hydroxyapatite and other ion substituted HA, tricalcium phosphate, tctracalcium phosphate, monetite, dicalcium phosphate, dicalcium phosphate dihydrate, octacalcium phosphate, or calcium sulfate) may also be used. The effect of the processing parameters on fiber diameter has been carefully studied and the polymer molecular weight and dope concentration greatly affected fiber diameters ranging from 50 nm to 500 nm.
[0029J In order to fabricate the above-discussed scaffolds. HA particles were added to a PLLA solution to fabricate an HA/PLLA composite. Also, the amount of HA in the PLLA solution was adjusted by varying the HA to PLLA feeding ratio in the spin-dope.
[0030] According to certain embodiments of the present invention, up to approximately 20 wt% of HA is incorporated into PLLA nanofibcrs. These HA particles are typically well aligned along the long axis of the polymer fibers. The size of the HA particles have an average width of at least 10 nm and an average length ranged from approximately 10 nm to approximately 10 μm. with an average aspect ratio up to approximately 50. The particles, according to certain embodiments of the present invention, were homogcnously distributed within the PLLA nanofibers after elcctrospinning. The resultant microstrυcture closely mimicked the arrangement of the inorganic/organic components in ECM of natural bone. Compared to the fibrous scaffold fabricated with pure PLLA, the HA/PLLA scaffold has improved mechanical properties and biocompatibility.
[0031 ] As illustrated in FIGS. l(a)-(c), HA particles with lengths between 100 and 200 nm and aspect ratios between 7 and 10 were evenly distributed within HA/PLLA fiber bodies. These particles also demonstrated a good orientation along the long axes of the PLLA nanofibers.
[0032] The effects of altering the electrospinning processing parameters on the diameter of HA/PLLA composite fibers were studied in FIG. 2. As illustrated, varying the polymer concentration of the electrospinning dope and varying the HA/PLLA weight ratio have the most obvious influences on the diameter. As illustrated in FIG. 2(a), the diameter of composite nanofibers increase with the PLLA concentration in the spinning dope, which indicates that a high PLLA concentration dope has higher surface tension and was more di ITicult to be spun into finer fibers during the fiber spinning process. FIG. 2(b) illustrates that composite fiber diameter decreases with increasing HA weight ratio. This is likely explained by the fact that higher amounts of HA in a composite decreases the viscosity of the electrospinning dope and the surface tension thereof as well.
[0033] As illustrated in FIG. 3, several modified electrospinning techniques for fabricating functionalized nanofibrous scaffolds have been implemented according to various embodiments of the present invention. First, a PEG/PLLA core-shell structure has been co-elcctrospun into a fibrous composite scaffold using co-axial dual spinnerets, as shown in FIG. 3(a). Second, highly aligned nanofibers were fabricated using a rotating drum as the collector, as illustrated in FIG. 3(b). Scaffolds prepared in this manner have good orientation and improved mechanical strength along the long axes of the fibers. Third, nanofibers with a porous surface have been electrospun using a mixture of CH2Cl2 (DCM) and DMF (DCMZDMF= 6/1 (v/v)) as a solvent, as illustrated in FIG. 3(c).
(0034] As illustrated in FIG. 3, the porosity and pore size of fibers according to certain embodiments of the present invention can be adjusted by altering the solvent used and dope concentration. The porous surface of the fibers can be used, for example, for controlled deliver)' of growth factors. Moreover, the porous surface will also enhance the bonding strength between the polymer fiber and a biomimetic apatite coating, such as the one illustrated in FIG. 8(b). The porous surface may also act as a nucleation site for apatite further growth.
[0035] FIG. 4 compares mechanical properties of various electrospun composite fibers with different HA incorporation ratios. One of skill in the art will recogni7.c. upon analyzing FIGS 4(a)-(c), that both the Young's moduli and tensile stresses of the electrospun mats increased continuously as the HA incorporation ratio increased. This can be explained by the fact that HA, when well dispersed and aligned along fiber long axes, plays a substantial role in reinforcing the composite fibrous mat. Comparisons between the data included in FIG. 4(b) and FIG. 4(c) also demonstrates that fibrous mats with thicker composite nanofibers present more desirable mechanical properties.
[0036] FIG. 5(a)-(b) illustrates the mechanical properties of electrospun PLLA-based scaffolds using tensile test. One of skill in the art will recognize that both the alignment of the scaffold assembly and the incorporation of nano-sizc. needle-shape HA particles into the nano-fibers significantly improved the clastic modulus of the composite scaffold. The scaffold with HA particles are much stiffer than those without HA particles, and the elastic modulus of the former is more than two times as high as that of the later. It is also shown that, the HA nanoparticles inhibit un-folding and orientation of PLLA molecular chains within spun fibers during tensile testing, i.e. cold drawing of the scaffold, and decrease the toughness of the composite scaffolds by decreasing their elongation at break.
[0037] According to certain embodiments of the presentation, the elastic modulus of the scaffolds with aligned assembly is four to five times higher than those with random fibrous assembly. Moreover, the pure PLLA scaffold with aligned assembly has much higher toughness but lower elongation at break than those with a random assembly. In the case of HA/PLLA scaffolds, such difference is not as significant as that of the pure PLLA scaffolds.
[0038] According to certain embodiments of the present invention, a homogenous apatite coating layer can also be formed on the surface of both PLLA and HA/PLLA scaffolds, as shown in FIGS. 8(a)-(b). The thickness of the coating is a few micrometers, which was obtained after approximately 4 hr of immersion in a modified simulated body fluid (m-SBF). The thickness of the coating can be adjusted by varying the Ca and P ion concentrations in SBF, sample immersion time, and pH of the solution.
J0039] According to certain embodiments of the present invention, maintaining the immersion time short is important in order to maintain the integrity of the polymer fibers. According to some of these embodiments, some polymer fibers absorb water, which leads to the reduction of their mechanical properties. Nevertheless, the thickness of the coating can be adjusted by varying the coating conditions such as, the m-SBF pH, immersion time, and calcium and phosphorous concentrations.
[0040 J According to certain embodiments of the present invention, fluorescein isothiocyaπatc labeled bovine serum albumin (F1TC-BS.A) was incorporated into the biomimetic apatite coating formed-on the surfaces of the scaffold, to study the drug release behaviors of the electrospun scaffolds. The drug release profiles of the electrospun HA/PLLA fibrous scaffolds are shown in FlG 9. The release of FITC-BSA from the biomimetic coating on the scaffold was studied for a time period of 8 weeks. Sustained release profiles have been observed for all scaffolds. The scaffolds with nano-si7.e HA particles (NHA) showed faster release profiles than those incorporated with micro-size HA particles (MHA). Also, a slightly faster release has been observed for the scaffolds with an aligned assembly than those with a random assembly. According to certain embodiments of the present invention, the biomimetic coating formed on the electrospun scaffold can be an effective carrier for sustained release of proteins and/or drugs.
[0041 j According to certain embodiments of the present invention, PLLA-based electrospun scaffolds with different HA particles were used for in vitrυ cell culture study. Rat osteosarcoma cell line ROS 17/2.8 was used. FIG. 10 illustrates relative cell viability on different scaffolds. According to certain embodiments of the present invention, with the increase of the cell culture time, more cells were attached to the surface of the scaffold. Especially, more cells were found on the scaffolds incorporated with either nano- or micro-size HA particles than those on pure PLLA scaffold after 7 days of culture. After 10 days, such difference became much more significant.
[0042J According to certain embodiments of the present invention, the cell alkaline phosphatase (ALP) activities, an early marker of bone formation, on different scaffolds are shown in Figure 11. According to certain embodiments of the present invention, after 10 days of culture, almost all the HA incorporated scaffolds showed significantly higher ALP activities than the control, pure PLLA scaffold. These results collectively suggested that hydroxyapatite has improved the biocompatibiliry and cell signaling properties of the scaffold, which could make the scaffold a better material for bone fracture repair.
[0043] According to certain embodiments of the present invention, a thicker apatite coating was obtained for the HA/PLLA scaffolds than the pure PLLΛ scaffolds with the same SBF soaking time. This may be explained by the fact that some of the HA particles loaded in the PLLA fibers position themselves on the surfaces of the fibers and act as nucleation sites for the apatite coating growth. Also, according to certain embodiments of the present invention, the coating grew more effectively on the top surface than the interior for both pure PLLA and HA/PLLA scaffold.
[0044] HA/PLLA composite fibrous scaffolds that include micro-scale pores throughout the body of the scaffold owing to electrospinning are also within the scope of the present invention. Such scaffolds, according to certain embodiments of the present invention, include nanometer-size pores on the surface of fibers in the scaffold owing to an evaporation process of highly volatile solvent. In such embodiments, nanoporous surfaces on composite fibers in the scaffold not only contribute to better bonding between a fiber substrate and an HA coating applied through a biomimetic coating method, but also induce fast degradation of the composite fibers.
[0045] According to certain embodiments of the present invention, in order to promote a more homogenous apatite coating throughout the scaffold, a pumping device is used to assist ra-SBF penetrating into the scaffold or to create relatively large pores in the scaffold. Pores in the range of hundreds of micrometers, according to certain embodiments of the present invention, arc desirable for both the invasion of blood vessels to provide the necessary nutrient
SUDDIY to the transplanted cells and the bone formation. [0046] Other embodiments of the present invention include HA/PLLA composite fibrous scaffolds that include at least one composite fiber surface and an HA coating on the composite fiber surface. According to some of these embodiments, the coating is formed by using a biomimetic coating method. Also, the obtained HA coating layer on the fiber surface will typically not only increase the HA component within the scaffold and contribute to improved mechanical properties of the scaffold, but will also increase the exposure of HA to the surrounding tissue during in vivo application. Such exposure can improve the biocompatibility as well as the osteoconductivity of die composite scaffold.
[0047] Also according to certain embodiments of the present invention, a HA/PLLA composite fibrous scaffold is provided that includes poly-lactic-co- glycolic acid (PLGA) microspheres incorporated among fibers. According to some of these embodiments, the size of the microspheres is controlled to be above 100 micrometers. This typically not only increases the mechanical properties of the fibrous scaffold but may also be used as a carrier for releasing one or more different drugs.
[0048] Using scaffolds such as the ones discussed above, a method of multi-drug delivery may be implemented. For example, two or more different drugs may be preloaded into different components of an elcctrospinning composite dope and PLGA microspheres to form a composite fibrous scaffold Then, the drugs may be subsequently controllably released.
[0049] The many features and advantages of the invention arc apparent from the detailed specification, and thus, it is intended by the appended claims to cover all such features and advantages of the invention which fall within the true spirit and scope of the invention. Further, since numerous modifications and variations will readily occur to those skilled in the art. it is not desired to limit the invention to the exact construction and operation illustrated and described, and accordingly, all suitable modifications and equivalents may be resorted to, falling within the scope of the invention.

Claims

3414CLAIMS What is claimed is:
1. An artificial composite structure, comprising: a fibrous matrix including a plurality of fibers; and a plurality of hydroxyapatite (HA) particles dispersed within the fibrous matrix, wherein the HA particles arc substantially aligned along long axes of the plurality of fibers.
2. The structure of claim 1, wherein the plurality of fibers have an average diameter of between approximately 50 nm and several micrometers.
3. The structure of claim 1 , wherein the HA particles have an average width of at least 10 nm and an average length of approximately 10 nm and 10 micrometers.
4. The structure of claim 1, wherein the plurality of fibers is selected from the group consisting of poly-(L-lactic acid) (PLLA), collagen, hyaluronans; fibrin, chitosan, alginate, animal-derived polymers, plant-derived polymers, PLA, PCL, PGA, synthetic polymers, natural polymers, polyesters, polycthcrs, polycarbonates, polyamines, polyamides, co-polymers, and combinations thereof.
5. The structure of claim 1, wherein up to 20 weight percent of HA particles are incorporated into the fibrous matrix.
6. The structure of claim 1, wherein the HA particles have aspect ratios between approximately 3 and approximately 50.
7. The structure of claim 1, wherein the plurality of fibers comprise a
Dolvethylcne glycol (PEG) core and a PLLA shell.
SUBSTITUTE SHEET (RULE 26
8. The structure of claim 1, wherein the plurality of HA particles form a coating layer on the plurality of fibers.
9. The structure of claim 1, further comprising: poly-lactic-co-glycolic acid (PLGA) microspheres incorporated among the plurality of fibers.
10. A method of forming a bone composite structure, the method comprising: adding hydroxyapatite (HA) particles to a poly-(L-lactic acid) (PLLΛ) solution to form a mixture; and forming an HA/PLLA fiber by electrospinning the mixture.
11. The method of claim 10, wherein the forming step comprises the fiber to have a diameter of between approximately 50 ran and several micrometers.
12. The method of claim 10, further comprising: forming the HA particles to be sized between approximately 10 run and approximately 10 micrometers.
13. The method of claim 10, further comprising: forming the HA particles to have aspect ratios of between approximately 5 and approximately 50,
14. Tlic method of claim 10, wherein the forming step comprises: utilizing substantially co-axial dual spinnerets during the electrospinning of the mixture.
i s The method of claim 10. wherein the forming step comprises: SUBSTITUTE SHEET (RULE 26 utilizing a rotating drum as a collector during the electrospinning of the mixture.
16. The method of claim 10, further comprising: immersing the fiber in a modified simulated body fluid (m-SBF) solution.
17. The method of claim 16, further comprising: pumping the m-SBF through a pumping device during the immersing step.
18. The method of claim 16, further comprising: crcat large pores' by eiectrospinning to enhance mass transfer in the structure.
19. T he method of claim 10, further comprising: incorporating poly-lactic-co-glycolic acid (PLGA) microspheres among the plurality of fibers.
20. A structure, comprising: a fibrous matrix including a plurality of fibers; and a plurality of hydroxyapatitc (HA) particles dispersed within the fibrous matrix, wherein the HA particles arc substantially aligned along long axes of the plurality of fibers, wherein the structure is formed by adding the HA particles to a poly-(L-lactic acid) (PLLA) solution to form a mixture and by forming HΛ/PLLA fibers by electrospinning the mixture to form the fibrous matrix.
21. The structure of claim 19, wherein the HA particles have an average size of between approximately 10 nm and 10 micrometers.
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CA2680586A1 (en) 2008-10-02
US7879093B2 (en) 2011-02-01
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US20080292839A1 (en) 2008-11-27

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