KR20230039896A - Vaginal cleaning composition - Google Patents

Abstract

In one aspect, the provided vaginal cleaning composition can exhibit excellent cleaning ability while minimizing irritation to the female genital organs, and the vaginal cleaning agent and vaginal cleaning kit including such a composition are related to the female genital organs based on the excellent cleaning ability. It can exert an excellent effect in the prevention or treatment of a disease.

Description

Vaginal cleaning composition

It relates to a vaginal cleansing composition, a vaginal cleanser containing the same, and a vaginal cleansing kit.

Vaginitis is a common gynecological disease in women, accompanied by pruritus, pruritus, and/or peculiar smell. Such vaginitis is rarely seriously exacerbated, but is not eradicated even with treatment and is a disease in which recurrence occurs frequently. The main symptoms of patients with vaginitis are dysentery, vulvar itching, severe pain during urination, especially pain in the epithelium of the vaginal opening, urethra, and perineum. The vagina is easily invaded and propagated by various strains, such as bacteria, fungi, and protozoa, which are the causative agents of vaginitis, because the vagina is located deep in the human body, has high temperature and humidity, and is rich in nutrients. In other words, the vagina is a suitable environment for microorganisms such as bacteria, fungi, and protozoa to live, so vaginitis is highly likely to occur, and thus many people suffer from vaginitis.

Common vaginitis in women of childbearing age is Candida vaginitis, also known by various names such as Trichomonas vaginitis, Monilial or Fungal vaginitis, Hemophilus vaginitis, and herpes virus. and herpes virus vaginitis. Before puberty or after menopause, the epithelium of the vagina becomes thinner, so it is more easily infected by various causative bacteria such as gonorrhea and nonspecific bacteria.

Trichomonas vaginitis, one of the most common vaginitis, is caused by the flagellar parasite Trichomonas vaginalis. Even healthy people without vaginitis can have Trichomonas parasites in the vagina or bladder in women and in the urethra or semen in men. Symptoms of trichomonas vaginitis include dystrophy, nitrogenosis, burning pain, dyspareunia, bleeding, vaginal erythema, and pain during urination. Causes of trichomonas vaginitis include sexual disorder, poor public hygiene, and use of oral contraceptives, and the incidence and recurrence rate are increasing. Drugs such as metronidazole and flagyl are known as drugs for treating Trichomonas vaginitis. In recent years, resistance to Trichomonas protozoa has been increasing, and therefore, development of various drugs against resistant bacteria is in progress.

Candida vaginitis is the most common vaginitis along with trichomonas vaginitis, and it is vaginitis that affects about 10% of non-pregnant women and about 1/3 of pregnant women with symptoms of vaginal discharge. The main symptoms include pruritus and local itching, and other symptoms include burning sensation, dyspareunia, edema, and vulvar pain. The causative organism is Candida albicans, which is a fungus, and is infected by infected hands, towels, clothes, public baths, utensils, etc. As therapeutic drugs, ketoconazole, mycostatin, myconazole nitrate, clotrimazole, etc. are known to be effective.

Haemophilus vaginitis is known to be sexually transmitted and is therefore classified as a sexually transmitted disease (STD). The causative bacteria is Hemophilus vaginalis, and the main symptoms include itching and vaginal discharge with an unpleasant odor. Treatment methods include oral administration of terramycin and topical application of sulfa cream. Recently, flagyl is known as the most effective treatment.

In addition to the above vaginitis, there is herpetic vaginitis caused by the herpes virus. Herpes vaginitis is transmitted through oral sex or sexual intercourse with a herpes-infected person, and caution is required to cause cervical cancer. Herpes vaginitis, like other vaginitis, has symptoms of pruritus and abnormal coldness, accompanied by headache, malaise, and fever symptoms. Since it is a disease caused by the herpes virus, it is difficult to eradicate and has a characteristic that it can recur at any time if a good environment for the virus to proliferate is provided. Thus, treatment is aimed at alleviating the symptoms. As a treatment, you need to take a cream or medicine containing acyclovir, famciclovir, or viramid, and if there are no symptoms, drug treatment is unnecessary.

Vaginal washes used for the treatment and prevention of such vaginitis include trichlorocarban (trade name: Solbacta, Boryung Pharmaceutical), povidone iodine (trade name: Zinobetadine, Korea Pharma), and the like. However, the use of vaginal washes is not satisfactory in preventing or treating vaginitis, and therefore, it is necessary to develop more effective vaginal washes.

Korean Patent Publication No. 2012-0063076

An object of the present invention is to provide a vaginal cleansing composition, a vaginal cleanser containing the same, and a vaginal cleansing kit.

An object of the present invention is to provide a vaginal cleansing composition containing probiotics, a vaginal detergent containing the probiotics, and a vaginal cleansing kit.

One aspect provides a vaginal cleansing composition comprising sodium bicarbonate, glucose and potassium chloride.

20 to 70, 25 to 70, 30 to 70, 40 to 70, 20 to 65, 20 to 60, 20 to 55, 20 to 50, 20 to 45, 25 to 65 sodium bicarbonate, based on the total weight of the composition , 30 to 60, 35 to 55, 40 to 50, 40 to 45, or 35 to 50% by weight, and according to one embodiment, preferably 35 to 50% by weight, suitable cleaning ability , It may contain 40 to 45% by weight most preferably in terms of minimizing irritation to the genitals while exhibiting bactericidal ability and alleviating ability of itching, but is not limited thereto.

30 to 70, 35 to 70, 40 to 70, 45 to 70, 50 to 70, 30 to 65, 30 to 60, 30 to 55, 35 to 65, 40 to 60 or 45 glucose, based on the total weight of the composition to 55% by weight, and according to one embodiment, it may preferably contain 40 to 60% by weight, and it promotes the growth of Lactobacillus bacteria in the vagina at an appropriate level without excessive acidity in the vagina. In terms of maintaining the production of lactic acid, it may most preferably contain 45 to 55% by weight, but is not limited thereto.

Based on the total weight of the composition, 1 to 10, 1 to 9, 1 to 8, 1 to 7, 1 to 6, 1 to 5, 1 to 4, 1 to 3 or 1 to 2% by weight of potassium chloride may be included. And, according to one embodiment, it may preferably contain 1 to 10% by weight, and exhibits excessive alkalinity to prevent discomfort and irritation to the genitals, while maintaining appropriate alkalinity to exert a sterilizing effect, body fluids In terms of maintaining the balance of and effectively cleaning the quality, it may most preferably contain 1 to 2% by weight, but is not limited thereto.

The composition may further include “probiotics”.

In one embodiment, the composition may be a mixed fermented composition by probiotics.

In the present specification, probiotics refers to microorganisms that benefit the human body as a general term for live microorganisms that benefit the human body when ingested in an appropriate amount. Probiotics were defined in 2001 by the joint expert committee of the World Health Organization (WHO) and the Food and Drug Administration (FAO) as 'live microorganisms that, when ingested in appropriate amounts, are beneficial to health'. It is used as a term to refer to the probiotics that affect it. Most probiotics known to date are lactic acid bacteria, which collectively refer to bacteria that obtain energy by fermenting sugars and produce a large amount of lactic acid, and are also called lactic acid bacteria. Lactic acid bacteria or beneficial bacteria, which are probiotics, survive stomach acid and bile acid in the body, reach the small intestine, multiply and settle in the intestine. To exert beneficial effects on health within the colonized intestine, these probiotics must be non-toxic and non-pathogenic.

In one embodiment, probiotics may be used in a vaginal cleansing composition by using a culture medium of a specific bacteria or a metabolite of the bacteria.

In one embodiment, the vaginal cleansing composition containing probiotics has an effect of preventing, improving or treating vaginitis. For example, a mechanism to slow down skin aging by increasing the proportion of beneficial bacteria in the vaginal skin flora, or to find and reduce the proportion of harmful bacteria that adversely affect the vaginal environment in the vaginal flora to alleviate symptoms of vaginitis. can

In one embodiment, probiotics can enhance the efficacy of active substances acting on the human body by increasing the content of active substances in molecular units such as enzymes or peptides in the vaginal cleansing composition through metabolites.

In one embodiment, the probiotics may be lactic acid bacteria. The lactic acid bacteria are morphologically divided into cocci (Lactococcus, Pediococcus, Leuconostoc) and bacilli (Lactobacillus, Bifidobacterium), and are Gram-positive bacteria. Among them, Lactobacillus is roughly divided into two types, normal fermentation and abnormal fermentation, and it is classified into 55 bacterial species and 11 sub-bacterial species according to growth temperature, glycolysis, growth, and polarization of produced lactic acid. Lactobacillus is a representative lactic acid bacterium, which is used in various fermented foods, and also has an important relationship with the health of humans and animals as a resident flora of the intestinal tract.

Based on the total weight of the composition, 0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight.

In one embodiment, the composition may include 0.5 to 5% by weight of the lactic acid bacteria, and most preferably 0.5 to 1% by weight.

In one embodiment, the lactic acid bacteria may be Prolyte lactic acid bacteria, and the proline lactic acid bacteria may be a mixture of lactic acid bacteria with proline, which is a kind of amino acid, and may be a lactic acid bacteria composition with increased delivery rate and survival rate of lactic acid bacteria.

In one embodiment, 0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight, in one embodiment When following, it may preferably contain 0.5 to 5% by weight, and most preferably 0.5 to 1% by weight. If the composition contains proline lactic acid bacteria in excess of the above value, the stability of the vaginal cleansing composition may deteriorate, and if the value is less than the above value, the effect of maintaining vaginal acidity may deteriorate.

In one embodiment, when the proline lactic acid bacteria are contained in an amount of 0.5 to 2% by weight, the acidity in the vagina is kept constant to form a protective film of the skin barrier in the vagina to block the penetration of various contaminants and harmful bacteria from the outside. environment can be maintained.

According to the vaginal cleaning composition additionally containing proline lactic acid bacteria according to an embodiment, the lactic acid bacteria can be additionally supplied into the vagina while maintaining an appropriate alkalinity and exhibiting a bactericidal effect. As a result, the composition promotes the growth of lactic acid bacteria to maintain acidity in the vagina, so that the cleaning effect and protective effect of the vagina may be higher.

In one embodiment, the probiotics included in the composition can properly maintain the balance of the microbial community in the vagina by promoting the activity and survival of beneficial bacteria in the vagina while inhibiting the growth and/or activity of harmful bacteria in the skin.

The composition may additionally include herbal extracts. The herbal extract may help proline lactic acid bacteria settle in the vagina to maintain weakly acidic conditions.

The herbal extract may be at least one selected from the group consisting of lavender, houttuynia cordata, dimask rose, chamomile, green tea and calendula.

0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 based on the total weight of the composition to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight. According to one embodiment, it may preferably contain 0.5 to 5% by weight, and most preferably 0.5 to 1% by weight.

The composition may further include a combination of two or more amino acids. The amino acid may be one that helps improve immunity in the vagina. Specifically, the amino acid may improve the strength of the vaginal mucosa and consequently improve the function of the vaginal mucosa. The amino acid may be glutamine or alanine.

The composition may further include glutamine. The glutamine may improve the function of the vaginal mucosa by improving the strength of intercellular bonding in the vaginal mucosa or by adjusting the metabolism and cell proliferation of the vaginal mucosa.

Based on the total weight of the composition, 0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight. According to one embodiment, it may preferably contain 0.5 to 5% by weight, and most preferably 0.5 to 1% by weight. If the glutamine is higher than or lower than the above level, there may be a problem that the function of the vaginal mucosa is not sufficiently improved.

The composition may further include alanine.

0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 alanine, based on the total weight of the composition to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight. It may preferably contain 0.5 to 5% by weight, and most preferably 0.5 to 1% by weight.

The composition may further include a magnesium compound.

The type of the magnesium compound is not particularly limited as long as the magnesium compound can stabilize magnesium ion in the form of a salt and be included as an effective active ingredient in the composition. According to one embodiment, the magnesium compound may be magnesium lactate, magnesium phosphate, or a combination thereof.

0.5 to 10, 0.5 to 9.5, 0.5 to 9, 0.5 to 8.5, 0.5 to 8, 0.5 to 7.5, 0.5 to 7, 0.5 to 6.5, 0.5 to 6, 0.5 to 5.5, 0.5 to 5, 0.5 to 4.5, 0.5 to 4, 0.5 to 3.5, 0.5 to 3, 0.5 to 2.5, 0.5 to 2, 0.5 to 1.5 or 0.5 to 1% by weight, preferably according to one embodiment may contain 0.5 to 5% by weight. Most preferably, it may contain 0.5 to 1% by weight.

The vaginal washing composition may be a vaginal washing composition for relieving symptoms of vaginitis, assisting treatment of vaginitis, protecting cervical cells, or assisting treatment of HPV infection.

The vaginal cleansing composition may be used in combination with a vaginitis treatment agent.

The vaginal detergent may be used to help self-heal hpv infection. Specifically, the composition may be effective as an auxiliary treatment for hpv infection by administering the vaginal cleanser to the vagina of an hpv-infected individual at regular intervals for a long period of time to maintain vaginal acidity and increase immunity.

One aspect provides a vaginal cleanser comprising the composition.

The vaginal cleanser can exert a preventive or therapeutic effect by washing the vagina without particular limitation if it is a female genital organ, in particular, a woman's disease related to the vagina. Specifically, as for the vaginal-related female disease, there is no particular limitation as long as it is a vaginal-related disease that is reasonably expected by those skilled in the art to be preventable or treated during washing.

More specifically, as a female disease preventable or treatable by using the vaginal cleaner, it may be at least one selected from the group consisting of vaginitis, vaginal dryness, and vaginal laxity.

Specifically, the vaginitis is Candida vaginitis, called various names such as Trichomonas vaginitis, Monilial or Fungal vaginitis, Hemophilus vaginitis, and herpes virus It may be one or more selected from the group consisting of Herpes virus vaginitis.

The vaginal detergent may be used to help self-heal hpv infection. Specifically, the composition may be effective as an auxiliary treatment for hpv infection by administering the vaginal cleanser to the vagina of an hpv-infected individual at regular intervals for a long period of time to maintain vaginal acidity and increase immunity.

The vaginal cleanser may be formulated into a formulation suitable for topical administration, and may be formulated into any one formulation selected from the group consisting of a gel, lotion, topical liquid, ointment, tablet, or cream. Preferably, it can be formulated in a tablet form to be dissolved in a solvent and used in a gel or liquid form, and in this case, the degree of cleaning ability can be set in such a way that the user adjusts the number or concentration of dissolving tablets. There are advantages to being able to. Methods for preparing such topical preparations are widely known in the art, and, if necessary, amino acids, sodium bisulfite, sodium metabisulfite, sodium sulfite, disodium ethylenediamine tetraacetate, sodium bisulfide, sodium formaldehyde sulfoxylate Stabilizers such as thiourea, acetone sodium bisulfite, ceramide, glycerin, propylene glycol, ammonium alginate, cyclomethicone, dimethicone, polydextrose, sodium hyaluronidate, sodium lactate, sorbitol, triacetin, Moisturizers such as triethanolamine and xylitol, emulsifiers such as polyoxyethylene stearates, sodium benzoate, methyl p-hydroxybenzoate, (iso)propyl p-hydroxybenzoate, (iso)butyl paraoxybenzoate, sorbic acid, potassium sorbate, sorbin Pharmaceutically acceptable carriers or additives such as sodium acid, dihydroacetic acid, sodium dihydroacetate, benzalkonium chloride, benzentonium chloride, phenol, cresol, chlorocresol, and benzyl alcohol may be included.

One aspect provides a vaginal cleaning kit including the detergent and a vaginal washer.

The vaginal washer can help cleanly wash even invisible places by injecting the vaginal cleaning composition deep into the vagina of a woman, and generally requires a long nozzle for this purpose, and the cleaning composition passes through the long nozzle Therefore, in order to apply it to the inside of the vagina, a container that can apply a large force to push the cleansing water is required. The vaginal washer constituting the kit is not particularly limited, and any vaginal washer that can help to apply the vaginal cleaning composition well to the inside of the vagina and rinse well with water without threatening vaginal health can be used. can

The composition for vaginal cleansing according to one aspect can exhibit excellent cleaning ability of the female genital organs.

A vaginal cleansing composition containing probiotics according to another aspect can exhibit excellent cleansing ability while minimizing irritation to the female genital organs by the mixed fermentation of the composition by the probiotics.

According to another aspect, a vaginal cleanser and a vaginal cleansing kit containing the composition can exhibit excellent effects in preventing or treating female genital organs-related diseases based on the excellent cleansing ability.

1 is a graph measuring the antibacterial effect of vaginitis-inducing bacteria of a composition according to Preparation Example.

Hereinafter, examples will be described in detail for more detailed description.

1. Preparation of vaginal cleanser

(1) Preparation Example 1

A vaginal cleansing composition for intravaginal administration was prepared by adding an active ingredient to a buffer solution having a pH of 4.0 to 5.0 at room temperature in the amount shown in Table 1, leaving the active ingredient alone for one day, and mixing with a small amount of ethanol.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 Sum 100

(2) Production Example 2

In Preparation Example 2, a composition was prepared using the contents shown in Table 2 by the method according to Preparation Example 1, and then the composition was fermented through proline lactic acid bacteria.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 proline lactobacillus 0.75 Sum 100

(3) Preparation Example 3

In Preparation Example 3, a composition was prepared using the contents shown in Table 3 by the method according to Preparation Example 1, and then the composition was fermented through proline lactic acid bacteria.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 glutamine 0.75 proline lactobacillus 0.75 Sum 100

(4) Preparation Example 4

In Preparation Example 4, a composition was prepared using the contents shown in Table 4 by the method according to Preparation Example 1, and then the composition was fermented through proline lactic acid bacteria.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 alanine 0.75 glutanin 0.75 proline lactobacillus 0.75 Sum 100

(5) Production Example 5

In Preparation Example 5, a composition was prepared using the contents shown in Table 5 by the method according to Preparation Example 1, and then the composition was fermented through proline lactic acid bacteria.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 proline lactobacillus 0.75 glutamine 0.75 alanine 0.75 herbal extract 0.75 Sum 100

(6) Production Example 6

In Preparation Example 6, a composition was prepared using the contents shown in Table 6 by the method according to Preparation Example 1, and then the composition was fermented through proline lactic acid bacteria.

ingredient Content (% by weight) sodium bicarbonate 42.5 glucose 51.5 potassium chloride 1.5 proline lactobacillus 0.75 glutamine 0.75 Magnesium Lactate Magnesium Phosphate 0.75 alanine 0.75 herbal extract 0.75 Sum 100

2. Efficacy evaluation of the composition

(1) Measurement of acidity change in the vagina

In order to measure changes in vaginal health by the prepared composition, an experiment was conducted on 20 volunteers regardless of age. Specifically, using the compositions of Preparation Examples 1 to 6, the composition was used once a day for a total of 5 days at the same time. The use of the composition was carried out by a method of spraying the composition after sufficiently entering the vaginal cleaner device into the vagina. The average vaginal pH was measured before and after 7 days of using the composition.

Table 7 below measures the average pH in the vagina before and after use.

pH before use pH immediately after use pH after 7 days Preparation Example 1 5.7 4.8 5.7 Preparation Example 2 5.7 5.0 5.3 Preparation Example 3 5.7 5.1 5.2 Production Example 4 5.7 5.0 5.1 Preparation Example 5 5.7 4.9 5.0 Preparation Example 6 5.7 4.9 4.8

As a result, as shown in Table 7, the case containing proline lactic acid bacteria (Preparation Example 2) maintained a lower vaginal pH than the case without it (Preparation Example 1). Through this, it was found that the fermented composition including probiotics such as proline lactic acid bacteria was excellent in maintaining pH in the vagina.

In addition, when additional amino acids were added (Preparation Examples 3 and 4), the vaginal pH maintenance effect was better than when no amino acid was added, and when two specific types of amino acids were added (manufactured by Example 4) had a better vaginal pH maintenance effect.

In addition, the case of including the herbal extract (Preparation Example 5) had a better vaginal pH maintenance effect, and the case of the composition containing both proline lactic acid bacteria, the herb extract, a magnesium compound, and two amino acids (Preparation Example 6) It was confirmed that the effect was the best than when each was included.

These results indicate that proline lactic acid bacteria, herbal extracts, magnesium compounds, and two types of amino acids are mixed, and the synergistic effect of establishing lactic acid bacteria in the vagina and maintaining vaginal health is excellent.

(2) Evaluation of relief of vaginitis symptoms

In order to evaluate the inhibitory effect of the prepared composition on the major symptoms of vaginitis, pruritus, and specific odor, five volunteers in their 20s to 50s were recruited, and as a result, a total of 20 people were tested. Specifically, after using the prepared composition by the method of Example 1, the inhibitory effect on pruritus, pruritus and specific odor and satisfaction in terms of feeling of use (cleanliness, refreshment, etc.) were evaluated.

As for the evaluation method, satisfaction of 90% or more in terms of deterrence effect and usability was scored out of 5 points, 80% or more but less than 90% was 4 points, 70% or more and less than 80% was 3 points, 60% or more and less than 70% was 2 points, 50% or more and less than 60% were evaluated as 1 point, and less than 50%, where almost no effect was felt, was evaluated as 0 point. The score of each questionnaire was used by calculating the average value for each age group.

Table 8 below shows the average of the evaluation results of the participants for each preparation example.

manufacturing example polyglot itching peculiar smell feeling of use Preparation Example 1 3 2 3 2 Preparation Example 2 3 2 3 3 Preparation Example 3 3 3 3 3 Production Example 4 3 3 4 4 Preparation Example 5 4 4 4 3 Preparation Example 6 5 4 5 5

As a result, as shown in Table 8, the case of using Preparation Example 6 was more effective in improving cold soreness, pruritus and specific odor than the case of using other Preparation Examples, and it was confirmed that the feeling of use was also excellent. These results suggest that the composition of one aspect in terms of usability and functionality can give most of the raters an improvement in vaginal health.

(3) Comparison of improvement and treatment effect of vaginal dryness by type of vaginitis

When Preparation Examples 5 and 6 were used for candidal vaginitis, bacterial vaginosis, herpes, HPV, and menopausal vaginal dryness, improvement and treatment effects were evaluated.

As for the evaluation method, satisfaction of 90% or more in terms of deterrence effect and usability was scored out of 5 points, 80% or more but less than 90% was 4 points, 70% or more and less than 80% was 3 points, 60% or more and less than 70% was 2 points, 50% or more and less than 60% were evaluated as 1 point, and less than 50%, where almost no effect was felt, was evaluated as 0 point. The score of each questionnaire was used by calculating the average value for each age group.

Table 9 below shows the average of the evaluation results of the participants for each preparation example.

Candida vaginitis bacterial vaginosis herpes vaginitis HPV menopausal vaginal dryness Preparation Example 5 5 3 3 4 5 Preparation Example 6 5 3 4 5 5

As a result, as shown in Table 9, both Preparation Examples 5 and 6 were excellent in improving and treating candida and menopausal vaginal dryness, and among them, Preparation Example 6 was remarkably effective in improving the discomfort of HPV. , it was confirmed that the feeling of use was also excellent. These results suggest that the composition of one aspect can improve vaginal health and provide therapeutic effects to people suffering from diseases such as candidal vaginitis, HPV, and menopausal vaginal dryness.

3. Evaluation of the harmful bacteria killing effect of the composition

In order to evaluate the improvement and treatment effects when using Preparation Examples 1 to 6 for bacterial vaginosis, the harmful bacteria killing effect was confirmed for Hemophilus vaginalis.

The composition according to each preparation was treated by concentration, and after 48 hours, 10% EZ-Cytox (Daeil, 99-EZ3000) was mixed with the DMEM medium, and the sample-treated medium was suctioned and then dispensed at 100ul per well. did After culturing for 2 hours, the reduction rate of harmful bacteria in each medium was measured.

As a result, as shown in FIG. 1, in the case of Preparation Example 1, the survival rate of harmful bacteria was 75%, showing a reduction rate of 25%. On the other hand, in the case of Preparation Example 2, which was fermented by proline lactic acid bacteria, the survival rate of harmful bacteria was 53%, indicating a reduction effect of about 1/2.

In addition, when one type of amino acid was added (Preparation Example 3), the survival rate of harmful bacteria was lowered to 49%, and when two specific types of amino acids were added (Preparation Example 4), it was confirmed that the survival rate of harmful bacteria was lowered to 45%. did

In addition, in the case of including the herbal extract (Preparation Example 5), the survival rate of harmful bacteria was lowered to 38%, and in the case of the composition containing both proline lactic acid bacteria, herb extract, magnesium compound, and two amino acids (Preparation Example 6), the harmful bacteria The survival rate was reduced to 25%, confirming that the effect was the best.

These results suggest that the composition according to the present invention can improve vaginal health and provide therapeutic effects to people suffering from diseases such as Haemophilus vaginitis.

Claims (14)

A vaginal cleansing composition comprising sodium bicarbonate, glucose, potassium chloride, probiotics and amino acids.
The method according to claim 1, relative to the total weight of the composition, the composition comprising 35 to 50% by weight of sodium bicarbonate, 40 to 60% by weight of glucose, 1 to 10% by weight of potassium chloride.
The composition of claim 1 , further comprising an herbal extract.
The composition according to claim 1, further comprising 0.5 to 1% by weight of probiotics based on the total weight of the composition.
The composition according to claim 1, wherein the amino acids are two or more.
The composition according to claim 5, wherein the amino acids are glutamine and alanine.
The method according to claim 6, relative to the total weight of the composition, the composition further comprises 0.5 to 1% by weight of alanine or glutamine.

The composition of claim 1 , further comprising a magnesium compound.
The composition according to claim 8, wherein the magnesium compound is magnesium lactate, magnesium phosphate, or a combination thereof.
The composition according to claim 8, further comprising 0.5 to 1% by weight of a magnesium compound based on the total weight of the composition.
A vaginal cleanser comprising the composition of any one of claims 1 to 10.
The detergent according to claim 11, wherein the detergent is for prevention, treatment, or auxiliary treatment of vaginitis, vaginal dryness, vaginal atony, hpv virus, or a combination thereof.
12. The detergent according to claim 11, wherein the detergent is in tablet form.
A vaginal cleaning kit comprising the detergent of claim 11 and a vaginal washer.

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