JPS54110385A - Immobilization of enzyme - Google Patents
Immobilization of enzymeInfo
- Publication number
- JPS54110385A JPS54110385A JP1580878A JP1580878A JPS54110385A JP S54110385 A JPS54110385 A JP S54110385A JP 1580878 A JP1580878 A JP 1580878A JP 1580878 A JP1580878 A JP 1580878A JP S54110385 A JPS54110385 A JP S54110385A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- polymer
- carrier
- radical
- irradiation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 5
- 102000004190 Enzymes Human genes 0.000 title abstract 5
- 229920000642 polymer Polymers 0.000 abstract 4
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 abstract 2
- 239000000178 monomer Substances 0.000 abstract 2
- -1 polyethylene Polymers 0.000 abstract 2
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract 1
- 239000004698 Polyethylene Substances 0.000 abstract 1
- 108010046334 Urease Proteins 0.000 abstract 1
- 125000003172 aldehyde group Chemical group 0.000 abstract 1
- 150000001299 aldehydes Chemical class 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000004744 fabric Substances 0.000 abstract 1
- 239000010408 film Substances 0.000 abstract 1
- 239000008187 granular material Substances 0.000 abstract 1
- 229920000573 polyethylene Polymers 0.000 abstract 1
- 229920000139 polyethylene terephthalate Polymers 0.000 abstract 1
- 239000005020 polyethylene terephthalate Substances 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To prepare a water-insoluble immobilized enzyme having excellent storage stability, without lowering the enzymatic activity, by bonding an enzyme to a polymer carrier grafted with a radical-polymerizable aldehyde-containing monomer, with covalent bond.
CONSTITUTION: A radical-polymerizable monomer having an aldehyde group, such as acrolein, is grafted to a polymer by light-irradiation, ionizing ray-irradiation, etc., and an enzyme such as urease, etc., is bonded to the carrier with covalent bond. The polymer carrier is, e.g. polyethylene, cellulose, polyethylene terephthalate, etc., and its form is arbitrary, e.g. powder, porous granule, film, fabric, etc. The bonding of the enzyme to the polymer is effected by immersing the carrier in a buffer solution of the enzyme, and made to react for ≥2 hours at ≥10°C, pref. 4W5°C.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1580878A JPS54110385A (en) | 1978-02-13 | 1978-02-13 | Immobilization of enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1580878A JPS54110385A (en) | 1978-02-13 | 1978-02-13 | Immobilization of enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS54110385A true JPS54110385A (en) | 1979-08-29 |
Family
ID=11899137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1580878A Pending JPS54110385A (en) | 1978-02-13 | 1978-02-13 | Immobilization of enzyme |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS54110385A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01158054A (en) * | 1987-09-14 | 1989-06-21 | Gelman Sciences Inc | Film activated by polyaldehyde |
| US8497107B2 (en) | 2008-09-30 | 2013-07-30 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
-
1978
- 1978-02-13 JP JP1580878A patent/JPS54110385A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01158054A (en) * | 1987-09-14 | 1989-06-21 | Gelman Sciences Inc | Film activated by polyaldehyde |
| US8497107B2 (en) | 2008-09-30 | 2013-07-30 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
| US9187744B2 (en) | 2008-09-30 | 2015-11-17 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
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