JPH04214519A - Plastic slides for optical microscopes - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] [Field of Industrial Application] The present invention relates to a slide and a cover used for holding a sample when observing the sample with an optical microscope. It is designed to be used primarily for observing liquid samples.

[Conventional technology]

Conventionally, glass has been used for slides and covers for holding samples when using an optical microscope. The surfaces of the slide and cover were mostly planar, since it is quite difficult to process glass surfaces into shapes other than planar. Therefore, a drop of the sample is dropped onto a slide, a cover glass is placed on top of it, and the sample is observed under a microscope. However, the thickness of the liquid phase of the sample changes slightly each time, so complicated operations are required to keep it constant. Met. Furthermore, since the sample adhered to the periphery of the cover glass, it was difficult to avoid the sample from adhering to the fingertip.

In recent years, advances in plastics have led to the creation of plastic slides and covers. There are also models on the market where the slide and cover are integrally molded. The thickness of the liquid phase is constant because it is integrally molded, but the thickness is 80 to 100 μm.
It is very thick and unsuitable for high-magnification observation, and the structure is such that the liquid easily leaks to the outside and sticks to the hands of the speculum examiner. Furthermore, although there are scale lines on the outer surface of the cover, the scale lines cannot be observed together with the sample, as will be clear from the relationship with the depth of focus described below. Currently, plastic slides and covers are used for examinations at medical institutions, etc., but they are mainly used for preliminary examinations, and there are no slides and covers that can be used for detailed examinations.

Furthermore, Japanese Utility Model Application Publication No. 2-2711 discloses a slide in which a narrow square groove is cut on the top of a plastic slide, and the part surrounded by the groove is the part on which a sample is placed and observed. ing. The plastic cover is large enough to cover the area surrounded by the square groove and the surrounding grooves, and can be moved parallel to the slide surface by a guide provided on the slide surface.

When the cover is moved to cover the sample dropped on the square part of the slide, a liquid phase part is formed between the cover and the slide, and the excess sample falls into the groove around the square.

Although it is possible to form a liquid phase with a certain thickness using this method, it has the disadvantage that the cover must be moved with the fingertips, and the sample tends to adhere to the fingertips at this time.

[Problem to be solved by the invention]

When observing a liquid sample with an optical microscope, the droplet is usually placed on a slide glass and covered with a cover glass, but with this method it is difficult to maintain a constant thickness of the liquid phase. Cells in the sample may be destroyed by pressing down on the cover glass too much. Furthermore, when counting a specific target in a sample, the thickness of the liquid phase needs to be constant.

However, it is quite complicated to maintain a constant thickness of the liquid phase.
Furthermore, it was difficult to avoid the sample from adhering to the fingertips if such an operation was performed. Therefore, there is a great need for a method that allows the thickness of the liquid phase to be constant through simple operations and that prevents any sample from adhering to the fingertip.

Especially in recent medical tests, where there is a risk of hepatitis or other harmful viruses being mixed into the sample, it is important to prevent the sample leaking from the slide glass from adhering to the examiner or the human body. It has become a challenge.

Taking these points into consideration, the present invention makes it possible to make the thickness of the liquid phase of the sample constant using an extremely simple method, and also prevents leakage when setting the sample on a slide glass and during subsequent handling. The object of the present invention is to provide a slide and a cover for an optical microscope, which are free from the risk of being contaminated or directly attached to the human body. If necessary, in order to facilitate the counting of specific objects, a scale is provided on the slide so that the object and the scale can be observed at the same time. In the following description, a plastic slide and cover for an optical microscope will be collectively referred to as a plastic slide for convenience.

[Means to solve the problem]

The present inventors focused on the fact that by changing the slide and cover from conventional glass to plastic, it was possible to give the slide a fine shape that was difficult to create with glass. As a result of intensive research into the relationship between the two, the present invention was achieved.

Specifically, (1) In a structure in which the sample holding cell of an optical microscope is formed by a slide, a cover, and a partition wall, the slide and cover are made of transparent plastic plates, and the partition wall seals the left and right sides of the cell, and also separates the slide and cover. It has the function of maintaining constant spacing, and is characterized by having an opening for sample injection at the front of the cell, and a minute gas flow opening at the rear of the cell through which gas can flow but not liquid. A plastic slide for an optical microscope, and (2) a structure in which the sample holding slide, cover, and bulkhead of the optical microscope are made of plastic as one body, and cells for holding the sample are formed inside the slide; The length in the direction is shorter than the slide, the left and right sides of the cell are closed between the slide and the cover by partition walls, grooves are provided on the slide along the front and rear edges of the cover, and grooves on the front of the slide and The gap between the cover serves as a sample injection port, the gap between the groove at the rear of the slide and the cover serves as a gas communication port, and the sample holding cells are partitioned by the left and right partition walls and the front and rear grooves and are separated by a predetermined interval. Characteristic plastic slide for optical microscopes and (3) In the above structure, the length of the cover in the front and back direction is narrower than the slide, and the left and right sides of the cell are closed by partitions between the slide and the cover, and the front and rear parts of the slide are closed. is provided with a tapered part where the distance between the slide and the cover gradually widens toward the outside, and grooves are provided on the slide along the front and rear edges of the cover on the outside of the tapered part, The gap at the tip becomes a gas flow port, and the left and right partition walls and the front and rear tapered portions form a sample holding cell with a predetermined interval, and there is also a sample injection port leading to the groove at the front. This is a plastic slide for optical microscopes, characterized in that the front and rear edges of the slide are dams.

Furthermore, a plastic slide is provided with a ridge-like protrusion on the cover side along at least the rear edge of the cover to form a cell, a sample injection port on the front part of the cover, and a gas flow port on the rear ridge-like protrusion. It is. Alternatively, convex scale lines can be provided on the inner surface of the cells of the slide.

The present invention will be explained in detail below.

In the present invention, the slide and cover for holding the sample of the optical microscope must be made of plastic. Furthermore, in the inventions set forth in claim 2 and below, the partition walls of the cells also need to be made of plastic. The type of plastic is not particularly limited. A wide range of materials can be used. For example, thermoplastic plastics include polymethyl methacrylate resin, polystyrene resin, polycarbonate resin, polysulfone resin, polyester resin, nylon resin, ethylene acrylic resin, polyethylene resin, polypropylene resin, poly4-methylpentene-1 resin, and polyvinyl chloride. The thermosetting plastic may be a phenol resin, a furan resin, etc., or a mixture of these may be used as appropriate.

Some of these plastics contain plasticizers, so they must be selected in consideration of the properties of the sample, and furthermore, when heat sterilization is performed, heat resistance must also be taken into consideration.

For example, when sterilization is required, plastics with high heat resistance such as polycarbonate, polysulfone, and poly-4-methylpentene-1 are suitable.

The plastic used for the slide and cover should not only have high transparency but also high optical isotropy, especially 10
This is important when observing at a high magnification of 00x or higher. In general, many thermoplastic plastics have crystallinity and exhibit birefringence when stretched, so care must be taken to prevent birefringence from occurring during molding.

In recent years, plastic lenses have developed, and plastics with high transparency and optical isotropy have been developed, and lenses whose surfaces have been coated to further increase light transmission are now in use.

Recently, acrylic resins have been mainly used as materials for these optical plastics. These are also extremely excellent as raw materials for the plastics of the present invention.

Also, the slide and cover may be made of the same plastic material or may be made of different materials.

Although the material of the partition wall is not particularly limited, it is preferable to use plastic and integrally mold the partition wall with the slide or the cover, since this makes it easier to maintain a constant distance between the slide and the cover. It is also possible to use adhesive strips or adhesives as partitions. The shape and structure of the sample injection port at the top of the cell are not particularly limited. The sample is primarily a liquid, but a wide variety of substances can be used as long as they can be filled into the cell.

At the bottom of the cell, a minute gas flow port is provided through which gas can flow but liquid cannot pass through. The plastic slides and the like of the present invention are mainly used for liquid spectroscopy, and the most common applications are for testing blood, cells, urine, etc. in medical institutions. It is diluted with a medium used in the medical field. When the pore diameter of the communication port is 5 μm, air can pass through, but these solutions cannot pass through, and even when the pore diameter becomes 10-odd μm, almost no solution passes through. The relationship between adhesion and cohesive force depending on the degree of hydrophilicity and hydrophobicity of the solution and slide,
Depending on other physical properties such as the viscosity of the solution, there may be cases where almost no leakage occurs even if the thickness is about 1 mm. However, preferably 50
It is 0 μm or less, more preferably 100 μm or less. Therefore, when a sample is injected into the cell, the air inside the cell is discharged to the outside through the gas flow port, and the inside of the cell is replaced with the sample, but the sample is prevented from leaking to the outside.

Further, the flow port may have a shape such as a slit, a groove, a microporous structure, or the like.

Preventing the infection of harmful viruses such as hepatitis has become an important issue in recent examinations at medical institutions.

Next, the structure of the plastic slide of the present invention will be explained with reference to the drawings.

FIG. 1 is a plan view of one aspect of the plastic slide etc. described in claim 2, FIG. 2 is an enlarged cross-sectional view taken along line A-A, and FIG. 3 is an enlarged cross-sectional view taken along line B-B. This is what was shown. The width of the cover 2 is narrower than the front and back width of the slide 1 (FIG. 3), and there is a cell 5 (FIG. 2) formed between the slide and the cover, into which a sample is injected. The left and right sides of the cell are closed off by partition walls 3. In the band-shaped portion 5 between the partition walls, the distance between the slide and the cover is maintained constant;
The transmitted light of this part is observed under a microscope. The thickness of this part, that is, the thickness of the liquid phase of the cell, is appropriate to be 10 to 20 μm when observing at high magnification of several hundred times or more, taking into account the depth of focus and attenuation of transmitted light. When observing blood, urine, cells, etc., which are frequently used in institutions, at a magnification of 200 to 400 times, a thickness of about 40 to 50 μm is appropriate.

The area of the observation portion is appropriately selected depending on the purpose of use, but is often about 10 x 10 to 18 x 18 mm.

In addition, the thickness of the slide and cover is closely related to the performance of the microscope's condenser and objective lens, so JIS
The range is 0.5 to 1.5 mm for slides.
, the cover is 0.06-0.25 mm. These are selected according to the usage conditions, but the most commonly used cover thickness is JIS No. 1-S 0.15-0.18
It is said to be mm.

There are grooves 4 on the slides along the front and rear edges of the cover 2, respectively.
and 6 are provided. When the sample is injected into the groove 4, it instantly fills the cell 5 due to capillary action, and the excess accumulates in the grooves 4 and 6, leaving the sample 14 in the state shown by the collection of many dots in Figure 3. Become.

When the sample is accumulated in the groove, even if the sample evaporates a little, it is replenished from the groove to the cell 5 by capillary action, and no bubbles are generated inside the cell. Therefore, if the plastic slide of the present invention is used, samples can be prepared extremely easily. Note that 7 is a narrow slit that functions as the minute vent hole mentioned above, through which the air inside is released when the sample is injected, but even if the groove is filled with liquid or the slide is tilted. However, almost no sample leaks out. According to the test results, the interval is 1 for aqueous samples.
Approximately 0 to 20 μm is appropriate. When the sample is injected into the cell, the sample is in the state shown by the collection of many dots in FIG. Note that since the thickness of the liquid phase in the cell 5 is narrow, the sample is held with strong force in this region by capillary action. When observing at high magnification, the sample (liquid phase) is generally made thinner, so the ability to retain the liquid becomes stronger due to capillary action (adhesion of the liquid to the slide and cover).
A force acts to narrow the gap between the cover 2 and the cover 2. Furthermore, when performing precise observation at high magnification, a thin cover is required due to the design of the optical system of the microscope. The specific thickness of the cover is
Although it varies somewhat depending on the microscope and manufacturer, 0.17 mm is often specified at 1000 times. At this thickness, even the glass cover will warp to the slide side to some extent.
The liquid phase is thinner in the center of the cell, even more so in the case of plastic. This will be discussed later regarding the slide scale lines.

When storing a plastic slide with a sample injected into a cell as a preparation, the preparation can be easily prepared by pouring molten paraffin into the grooves 4 and 6.

When using a microscope, the sample inside the cell and the light transmitted through the slide and cover are magnified and observed by the optical system of the microscope. Therefore, this portion of the slide and cover requires high optical isotropy and flatness. However, due to advances in mold manufacturing technology and plastic molding technology, it is now possible to obtain molded products comparable to glass.

Although the plastic slide of the present invention is made in one piece, as is clear from the cross-sections shown in FIGS. 2 and 3, so-called integral molding is not possible. Therefore, after the slide part and the cover part are molded separately, for example, between the bulkhead 3 and the cover 2 or between the bulkhead 3 and the slide 1.
It is necessary to create an integral piece by fusing the space between them using ultrasonic waves or by bonding them with a plastic adhesive. In the present invention, "made as one piece" means such a structure.

When actually making a plastic slide, the cover is made by cutting a wide sheet into a rectangular shape, and the other parts (slide and bulkhead) are integrally molded using a mold.
It is most efficient to fuse or bond between the two.

FIG. 4 is a plan view of an embodiment of the plastic slide described in claim 3, FIG. 5 is a sectional view taken along line C-C, and FIGS. 6 and 7 are DD and E-E
This is an enlarged cross-sectional view of . Slide 1, cover 2, bulkhead 3, cell 5 and groove 6 shown in these figures
The structure of the is the same as that explained in Figures 1 to 3 above, and the relationship between the cell thickness and the observation magnification of the microscope, the area of the observation part of the cell, the thickness of the slide and the cover, and the explanation about the relationship between the cell thickness and the observation magnification of the microscope, , the function of instantaneously filling the cell, the operation of the slit 7 and the method of making the plastic slide are also exactly the same as those described with respect to FIGS. 1 to 3.

However, in the plastic slide of FIG. 4, a tapered portion 8 is provided between the cell 5 and the grooves 4 and 6, in which the distance between the slide and the cover gradually increases toward the outside. Here, the shape of the tapered portion may be, for example, a trumpet-like shape or any other shape as long as the shape allows the liquid to be sucked into the center of the cell by capillary action. Furthermore, there are slits 9 and 7 between the slide and the front and rear edges of the cover, respectively. This allows air to circulate, but
This is to prevent liquid from passing through. A sample injection port 10 communicating with the groove 4 is also provided. Furthermore, the front and rear edges of the slide 1 serve as weirs 13.

When a sample is injected into the sample injection port 10, it enters the groove 4 and instantaneously fills a portion 5 of the cell due to capillary action. At this time, the air inside the cell is discharged to the outside through the slit 7. If the amount of sample 14 injected is appropriate, it will be held inside the cell 5 in the state shown by the collection of many points in FIG. As is clear from this figure, the liquid is held so as to be sucked into the center of the cell, so when a small amount of sample evaporates, it flows from the taper 8 part into the cell 5 due to capillary action.
Since the sample is supplied to the cell, no air bubbles are generated inside the cell. In addition, even if the amount of sample injected is somewhat large or small, it will not leak because it is held in the cell 5 and taper 8 by capillary action, and even when the slide is tilted, it will not leak outside. This is one of the most important parts of the invention.

Furthermore, even if the sample leaks outside for some reason and adheres between the slits 7 and 9 and the weir 13, it will not spill outside because of the weir 13. Therefore, if the person handling the sample
If you hold the outside of the body, the sample will not come into direct contact with your body. Preventing the infection of harmful viruses such as hepatitis has become an important issue in recent examinations at medical institutions. By using the plastic slide of the present invention, virus infection can be prevented in an extremely simple manner even in such tests. This is one of the advantages of the invention.

Furthermore, when the amount of sample injected is large and has accumulated in grooves 4 and 6, if only the sample injection port is closed, only slits 7 and 9 will be open to the outside air, and the slits are narrow and even though air can flow through, the liquid will not flow. does not leak.

The slide of the present invention can be provided with convex graduation lines on the cell side surface of the slide 1. The convex cross section of the graduation line and the shape of the ridgeline may be of any shape. In order to be able to observe the image of the scale lines sharply, it is most preferable to have a shape in which the top section has an acute angle and a constant height, that is, a triangular pyramid shape. Further, the scale lines may be applied only to a part of the cell or to the entire surface. Further, the arrangement of the scale lines is preferably in the form of a grid as shown in FIG. 8 or in the form of parallel lines as shown in FIG. 9, but other shapes may be used. Note that if there is a risk of damaging the cell membrane of the sample due to the convex portion having an acute cross section at the top, it is necessary to caulk the top.

Furthermore, it is necessary to consider that the depth of focus of a microscope is very shallow. Specifically, it is about 5 to 8 μm at 300 times, about 1.5 to 2.5 μm at 700 times, and about 0.9 to 1 at 1000 times.
μm and 1 μm or less. In other words, since only an extremely narrow range can be seen, the scale line can be observed as a sharp straight line only when the focus is on the top of the scale line.

Even if the shape of the scale line is a triangular pyramid as described above, when the focus is on the middle of the pyramid, it becomes two parallel lines, and when the focus is above the top, the scale line cannot be observed. Since the height of the scale line is considered to be up to about one-third of the cell thickness under normal usage conditions, more than half of the scale line is an area where the scale line cannot be observed.

Therefore, as shown in Figure 10, if a conical protrusion is provided on a part of the scale line and its height matches the distance between the slide and the cover, the scale can be observed no matter which part of the cell is focused. . For example, when the focus is near the slide surface, the field of view of the microscope is as shown in Figure 11(a),
When the object is focused near the cover, it is observed as shown in FIG. 11(b).

Therefore, no matter which part of the sample is observed by moving the focal point up or down, the sample and clear scale can be observed at the same time.

Conventional plastic slides that are integrally molded have scales on the outside of the cells, but the scales cannot be observed at all under a microscope. This is obvious when considering the relationship between the depth of focus and the position of the scale described above. In the present invention, since the scale line is provided on the inner surface of the cell, it is not only possible to observe the sample clearly, but also because the scale line is shaped as shown in Figure 10, the scale can be seen no matter where the focus is placed on the cell. Samples can be observed simultaneously.

In the explanation of Figure 3, when a sample is injected into the cell, capillary action causes a force to narrow the distance between the slide and the cover, causing the cover to curve toward the cell, and the plastic cover curves to a greater degree than the glass. Said it was big. By making the height of the conical protrusion on the scale line in FIG. 10 the same as the thickness of the cell, and by caulking the top to prevent the cover from curving, the slide and cover can always be held parallel.

In addition, providing a ridge-like protruding portion on the inner surface of the cell is
This is very difficult to do with glass, and it is also expensive, making it impractical. Furthermore, although it is relatively easy to carve groove-shaped scale lines on the glass, as described above, the scale cannot be used effectively due to the depth of focus. However, once a plastic mold is processed in this way, it is easy to reproduce a large number of molded products. Therefore, it can be said that a slide with complicatedly shaped scale lines as shown in FIG. 10 has a structure that best utilizes the properties of plastic.

In Figures 13 and 14, blood was diluted with physiological saline using a slide with a convex shape, a triangular cross section, a line spacing of 0.3 mm, a width of 19 μm, and a height of 11 μm. These are photographs of red blood cells taken with a microscope and enlarged to 200 and 320 times. From this, it is recognized that both the red blood cells and the scale lines can be clearly observed, making it easier to count the red blood cells. Furthermore, since the height of the scale line is 11 μm, the scale line can be clearly observed even when the focal point is moved.

Furthermore, colored plastics can also be used in the present invention. Depending on the nature of the sample and the purpose of observation, it is often necessary to observe it through a filter. For example, a green filter is often used when observing cells. Using pre-colored plastic slides eliminates the need for filters.

Although it is possible to color a glass slide to give it a similar function as a filter, colored slides are rarely used because they are very expensive. However, coloring plastics is extremely easy and inexpensive.

In the plastic slide of the present invention, one cell can be provided on one slide, or two or more cells can be provided on one slide. Recently, a large number of microscopic examinations are being carried out in medical institutions and the like, so if a large number of cells are provided on one slide, the efficiency of the examination can be greatly improved.

In the embodiment described in claim 4, it is necessary to provide a ridge-like convex portion at least at the rear portion in order to seal the gap between the slide and the cover.

This convex portion may be provided on the cover side or on the slide side. In this embodiment, contrary to the embodiments described in Claims 2 and 3, the structure provided on the slide side is minimized, and more structures are provided on the cover side. The partition walls and the ridge-like protrusions are in close contact with each other to prevent sample leakage. Moreover, this ridge-like convex part can be provided not only at the rear part but also at the front part.

Fig. 15 is a plan view of an embodiment in which ridge-like convex portions are provided on the rear and front parts of the slide, Fig. 16 is a cross-sectional view taken along line A-A, and Figs. 17 and 18 are Diagrams are B-B and C
-C is an enlarged cross-sectional view. Partition wall 3 and ridged convex portion 15
For the intersection point, make a notch on either side and fit them together, or make the ridge-like protrusion the length that matches the spacing between the partition walls, or use a plastic material on one side so that it deforms when the slide and cover are combined. A wide range of materials suitable for this purpose can be used.

Further, a notch is provided in the ridge-like convex portion between the sample injection port 10 and the cell 5, and the sample is injected into the cell. Further, a minute gas flow port 16 is provided in the ridge-like convex portion at the rear, so that when a sample is injected into the cell, the air inside is released, but the sample does not leak outside. The specific size and function of the gap between the flow ports have already been described.

〔Effect of the invention〕

By using the plastic slide of the present invention, the thickness of the sample (liquid phase) to be observed can be made constant with extremely simple operations. In addition, since the sample is held between the slide and the cover by surface tension, there is no risk of leakage to the outside.

Recently, a large number of microscopic examinations are carried out on a daily basis in medical institutions, and prevention of infection by hepatitis viruses and the like has become an important issue. The slide of the present invention has no risk of leaked samples directly adhering to the examiner or other human body, and since it is made of plastic, samples containing dangerous substances can be incinerated together with the slide after use. Therefore, it is particularly suitable for such uses. This is especially true considering that in the past, fingertips were often damaged by cover glasses while using microscopes.

In addition, by providing a convex graduation line with an acute triangular cross section on the cell side of the slide, and a conical protrusion on the ridgeline as shown in Figure 10, the sample and scale will be aligned no matter where the focus is set. They can be observed at the same time.

Furthermore, by coloring the plastic slide, there is no need to use a filter, and by providing a large number of cells on one slide, inspection efficiency can be greatly improved.

[Brief explanation of the drawing]

FIG. 1 is a plan view of one embodiment of the plastic slide of the present invention, FIG. 2 is a sectional view taken along A-A, and FIG. 3 is a sectional view taken along B-B.
This is an enlarged cross-sectional view of . FIG. 4 is a plan view of one embodiment of the plastic slide of the present invention, FIG. 5 is a sectional view taken along line C-C, and FIGS.
The figures are enlarged cross-sectional views along D-D and E-E, respectively. FIGS. 8 and 9 show plan views of scale lines provided on the cell side surface of the slide. FIG. 10 shows a front view and a plan view of a portion of one scale line. Figure 11(a) is the 8th
This is an image of the field of view obtained by observing the part near the sliding surface of the cell (focusing on the ridge line of the convex part of the scale line) with a microscope when the scale line in the figure has the shape as shown in Figure 10. FIG. 11(b) shows an image of the visual field near the cover. FIG. 12 shows a plan view of a slide etc. in which a large number of cells are provided on one slide. Figures 13 and 14 show red blood cells in blood diluted with physiological saline, which were photographed under a microscope using a slide with convex graduations and triangular cross-sections, enlarged to 2x and 200x and 320x, respectively. This is a photograph of red blood cells and scale lines. FIG. 15 is a plan view of one aspect of the plastic slide etc. described in claim 4, and FIG. 16 is a
17 and 18 are BB and C-
An enlarged cross-sectional view of C is shown. 1...Plastic slide 2...Plastic cover 3...Cell partition walls 4, 6...Cell grooves 5...Cells 7, 9...Slit between slide and cover 8...Tapered portion between slide and cover 10...Sample injection port 11 ...Graduation line 12...Image of the scale line visible under a microscope 13...Weir 14...Sample 15...Ridged convex portion 16...Gas flow port Applicant Kuraray Co., Ltd. Agent Patent attorney Ken Honda

Claims (5)

[Claims] Claim 1: A sample holding cell of an optical microscope comprises a slide;
In a structure formed by a cover and a partition, the slide and cover are made of transparent plastic plates, and the partition has the function of sealing the left and right sides of the cell and maintaining a constant distance between the slide and the cover, and the front part of the cell. A plastic slide for an optical microscope, characterized by having an opening for sample injection in the cell, and a minute gas flow port at the rear of the cell through which gas can flow but not liquid. 2. A structure in which the sample holding slide, cover, and partition of an optical microscope are made of plastic, and cells for holding the sample are formed inside them,
The length of the cover in the front and back direction is shorter than the slide, the space between the slide and the cover is closed on the left and right sides of the cell by a partition wall, and grooves are provided on the slide along the front and rear edges of the cover,
The gap between the groove at the front of the slide and the cover serves as the sample injection port, and the gap between the groove at the rear of the slide and the cover serves as the gas flow port. A plastic slide for optical microscopy characterized by having a holding cell. 3. A structure in which a sample holding slide, a cover and a partition wall of an optical microscope are made of plastic and a cell for holding a sample is formed inside them,
The length of the cover in the front and back direction is shorter than the slide, the space between the slide and the cover is closed on the left and right sides of the cell by a partition wall, and the gap between the slide and the cover is tapered at the front and rear of the slide so that it gradually widens toward the outside. A groove is provided on the slide along the front and rear edges of the cover on the outside of the tapered part, and the gap between the slide and the tip of the cover becomes a gas flow port, and the gap between the left and right partition walls and the front and rear The tapered part forms sample holding cells separated by a predetermined distance, and there is also a sample injection port that communicates with the groove at the front, and the front and rear edges of the slide serve as weirs. A plastic slide for optical microscopy. 4. A structure in which the sample-holding slide, cover, and partition of an optical microscope are made of plastic as one body, and cells for holding the sample are formed inside them,
The length of the cover in the front and back direction is shorter than the slide, and the space between the slide and the cover is closed on the left and right sides of the cell by a partition wall, and at least the rear part of the cover is provided with a ridge-like protrusion that seals the gap with the slide. The space between the partition wall and the ridge-like protrusion is sealed,
The left and right partition walls and the ridge-shaped protrusions form partitioned sample holding cells with predetermined intervals, and a sample injection port is provided at the front of the cover, and gas flows through the ridge-shaped protrusions at the rear. A plastic slide for optical microscopes characterized by being provided with minute gas flow holes through which liquids cannot pass through. 5. Claims 1, 2 and 3, wherein a convex scale line is provided on the cell side surface of the slide.
Plastic slides for optical microscopes as described in Section 1.