JPH02305554A - Liquid sample trace amount sampling tube - Google Patents

Abstract

PURPOSE:To prevent the occurrence of breakage due to an impact and to facilitate handling of a sampling tube by a method wherein a trace amount sampling tube to sample a liquid inspection sample by using capillary phenomenon is formed by using synthetic resin, and hydrophilic treatment is applied on the inner surface of the sampling tube. CONSTITUTION:A liquid sample sampling tube 1 is formed with a fine tube 2 made of synthetic resin, and polymethyl methacrylate having light transmission being high enough to allow confirmation of a liquid sample in the tube from the outside is preferably used as synthetic resin. Further, a hydrophilic material 3 is adhered on the inner surface of the fine tube 2. A block copolymer of polyethyleneglycol and polypropyleneglycol is listed as the hydrophilic material, and is preferably a substance the molecular weight of which is 800-2000 and which is polyethyleneglycol having weight ratio of 30-85%. After a solution in which 0.05-1.0 wt% a hydrophilic material is dissolved in a solvent is sucked and sprayed in the fine tube 2, the fine tube is dried.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a liquid sample micro-collection tube for collecting a micro-volume of a body fluid sample such as blood or urine using capillarity for use in testing.

[Prior Art] Conventionally, small amounts of liquid samples such as blood and urine have been collected using capillary action for clinical tests, such as biochemical tests and serum tests. For this collection, micro-volume collection tubes such as capillary tubes and capillary tubes are used.

Micro-volume collection tubes generally have an inner diameter of 0.5 to 1.83.
A glass tube of about 111 is used. Micro-sampling tubes are used, for example, when making a wound on the sole of an infant's foot with a scalpel and collecting blood from the wound.
It is used to make a wound on the tip of a finger with a scalpel and collect blood from the wound.

[Problems to be Solved by the Invention] However, the above-mentioned glass collection tube has a problem in that it is easily broken when subjected to impact, such as when dropped, and is difficult to handle.

Accordingly, the present invention provides a liquid sample collection tube made of synthetic resin that is easy to handle and is not damaged by impact caused by dropping or the like.

In particular, the present invention provides a liquid sample collection tube that has sufficient sample collection power due to capillary action even if it is made of synthetic resin.

[Means for Solving the Problems] What achieves the above purpose is a liquid sample micro-collection tube for collecting a liquid test sample using capillary action,
The collection tube is made of synthetic resin, and further includes:
The inner surface of the collection tube is a liquid sample collection tube whose inner surface has been treated to be hydrophilic.

Furthermore, it is preferable that the hydrophilic treatment is performed using a hydrophilic substance attached to the inner surface of the collection tube. Further, it is preferable that the synthetic resin is polymethyl methacrylate. Furthermore, it is preferable that the hydrophilic substance is a block copolymer of polyethylene glycol and polypropylene glycol. Furthermore, the block copolymer of polyethylene glycol and polypropylene glycol preferably has a molecular weight of 800 to 2000 and a weight ratio of polyethylene glycol of 30 to 85%.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The liquid sample micro-volume collection tube of the present invention will be explained using an embodiment shown in the drawings.

The liquid sample micro-volume collection tube 1 of the present invention is for collecting a liquid test sample using capillary phenomenon, and the collection tube 1 is made of synthetic resin, and the inner surface of the collection tube is made hydrophilic. being processed.

Therefore, an embodiment of the liquid sample micro-volume collection tube of the present invention will be described in the first embodiment.
This will be explained with reference to the figures and FIG.

FIG. 1 is an external view of the liquid sample collection tube of the present invention, and FIG. 2 is a sectional view taken along line I-1 in FIG. 1.

The liquid sample collection tube 1 of the present invention is formed of a thin tube 2 made of synthetic resin, the inner surface of which has been treated to be hydrophilic.

The synthetic resin thin tube 2 has an inner diameter of about 0.5 to 1.8i+x,
It is a tubular body having a length of about 50 to 150 ix and a wall thickness of about 0.3 to 1.0 xx, and is formed by cutting a continuous body of a tubular body formed by extrusion molding, or by injection molding. The synthetic resin used for the thin tube 2 preferably has a light transmittance that allows confirmation from the outside that a liquid sample such as blood has flowed into the thin tube. Therefore, the thin tube 2 is translucent, more preferably colorless and transparent. It is preferable that the thin tube 2 has a certain degree of strength. The specific strength is 501! It is preferable that the tube has a breaking strength of 0.2 kg or more when the tube is set on the jig inside and a load is applied to the center at a constant speed of 25 ax for 1 minute.

From this point of view, polymethyl methacrylate is preferable as the synthetic resin used for the thin tube 2. moreover,
It is preferable to have high strength against various solvents such as alcohol, and solvent-resistant polymethyl methacrylate containing 30 to 60% of an acrylic rubber component is particularly preferable.

The inner surface of the synthetic resin thin tube 2 has been subjected to a hydrophilic treatment, and the hydrophilic treatment is performed, for example, by adhering a hydrophilic substance 3 to the inner surface of the thin tube. As for the degree of hydrophilization, it is preferable that when the capillary tube 2 is vertically erected and the lower part of the capillary tube is slightly touched on the water surface, water can be sucked up by 5xz or more within 5 seconds.

By performing such hydrophilic treatment, the thin tube 2 has sufficient liquid suction power due to capillary phenomenon.

As the hydrophilic substance 3, it is preferable to use a surfactant that does not affect the test and does not significantly cause hemolysis, and preferably one that is difficult to dissolve in the liquid sample.

Examples of such hydrophilic substances include block copolymers of polyethylene glycol and polypropylene glycol.

Furthermore, as a block copolymer of polyethylene glycol and polypropylene glycol, the molecular weight is 800.
2,000 and the weight ratio of polyethylene glycol is more preferably 30 to 85%. Molecular weight is 80
If the molecular weight is 0 or more, hemolysis will not occur, and if the molecular weight is 2000 or less, the inner surface of the thin tube 2 can be reliably made hydrophilic. Furthermore, it is preferable that the proportion of polyethylene glycol is about 30 to 85%, since a reliable hydrophilic effect can be exhibited.

The adhesion of the hydrophilic substance to the inner surface of the thin tube 2 is achieved by dissolving the hydrophilic substance in a solvent (for example, an alcohol such as methyl alcohol or ethyl alcohol, or water, or a mixture of alcohol and water). 0.05~1.0
A solution dissolved in approximately % by weight is brought into contact with the inner surface of the capillary tube 2 by suctioning and injecting it into the capillary tube 2, and by spraying the solution in the form of a mist. Then, after draining the excess solution,
This is done by sending natural air or air into the inside of the tube to dry it.

The preferable amount of adhesion per unit area is 0.01μg/c
, wffi~1μ97c1.

Furthermore, if necessary, fine glass powder with a coagulation promoting effect, EDTA-2, anticoagulants such as heparin and sodium fluoride, glycolytic inhibitors, and blood cell stains (such as brilliant cresyl blue) are added. etc.) in the above-mentioned hydrophilic substance solution, or after the hydrophilic substance has been deposited, the solution may be introduced and deposited.

[Example] Next, an example of the liquid sample micro-volume collection tube of the present invention will be described.

(Example) Solvent-resistant polymethyl methacrylate [40% acrylic rubber component, trade name GR1240, manufactured by Kyowa Gas Chemical Industry Co., Ltd.] was extruded and cut to give an inner diameter of 1.5xz and an outer diameter. 2゜5Rj! , length IL
A thin tube made of Oxg synthetic resin was prepared.

As a hydrophilic substance, a polyethylene glycol-polypropylene glycol block copolymer (polyethylene glycol is A1, polypropylene glycol is B, A
A BA type block copolymer, molecular weight 1200, polyethylene glycol content 40% by weight, manufactured by Asahi Denka Co., Ltd.) was used.

Then, a 0.1% by weight solution of the above copolymer (solvent ethanol) is prepared, and the end of the above thin tube is immersed in the copolymer solution, and by suctioning from the other end, it is inside the thin tube. The solution was allowed to flow. After the solution was brought into contact with the inner surface of the thin tube, it was discharged and dried to adhere a hydrophilic substance, thereby creating a liquid sample collection tube of the present invention.

Using the collection tube of this example, when water was vertically sucked up by the capillary action of the collection tube, water could be sucked up approximately 15xz. Furthermore, when blood was similarly sucked up by capillary action, approximately 13 x m could be sucked up. Then, the end of the sampling tube from which the blood was collected was inserted into putty for capillary tubes (trade name: xx-vcs35, manufactured by Terumo Corporation) and sealed. After the blood inside the collection tube with its end sealed in this manner had coagulated (after being left for about 60 minutes), it was placed in a centrifuge and centrifuged. Note that the collection tube was not damaged at this time. Then, the collection tube was cut at the serum portion, and the serum was collected. At this time, there was no adhesion of blood clots or hemolysis to the inner surface of the collection tube, and no influence on test values was observed.

(Comparative example) Similar to the example, solvent-resistant polymethyl methacrylate and 40% acrylic rubber component were blended, product name: G R1240.
, manufactured by Kyowa Gas Chemical Industry Co., Ltd.) is extruded,
By further cutting, the inner diameter is 1.5 and the outer diameter is 2.5.
A thin tube made of synthetic resin with a diameter of 5 x m and a length of 110 H was prepared.

When water was vertically sucked up by capillary action using this thin tube, only about 2xz of water could be sucked up. Furthermore, when blood was similarly sucked up by capillary action, only about 1 inch of blood could be sucked up.

[Effects of the Invention] The liquid sample micro-volume collection tube of the present invention is a liquid sample micro-volume collection tube for collecting a liquid test sample using capillary action, and the collection tube is made of synthetic resin, Since the inner surface of the collection tube has been treated to make it hydrophilic, it is easier to handle than a glass collection tube because it will not be damaged even if it is subjected to impact, such as when dropped. Furthermore, even if it is made of synthetic resin, the inner surface has been treated to make it hydrophilic, so liquid samples such as blood and urine can be easily and reliably collected by capillary action.

[Brief explanation of drawings]

FIG. 1 is an external view of an embodiment of the liquid sample collection tube of the present invention, and FIG. 2 is a sectional view taken along line 1-1 of the liquid sample collection tube of FIG. ■...Liquid sample small amount collection tube 2...Synthetic resin thin tube 3...Hydrophilic substance

Claims (5)

[Claims] (1) A liquid sample micro-volume collection tube for collecting a liquid test sample using capillary action, the collection tube is made of synthetic resin, and the inner surface of the collection tube is treated to be hydrophilic. A liquid sample micro-volume collection tube characterized by: (2) The liquid sample micro-volume collection tube according to claim 1, wherein the hydrophilic treatment is performed by a hydrophilic substance attached to the inner surface of the collection tube. (3) The liquid sample micro-volume collection tube according to claim 1 or 2, wherein the synthetic resin is polymethyl methacrylate. (4) The liquid sample micro-volume collection tube according to claim 1 or 2, wherein the hydrophilic substance is a block copolymer of polyethylene glycol and polypropylene glycol. (5) The block copolymer of polyethylene glycol and polypropylene glycol has a molecular weight of 800 to 2.
000, and the weight ratio of polyethylene glycol is 30
5. The liquid sample micro-collection tube of claim 4, wherein the liquid sample micro-collection tube is a polyethylene glycol-polypropylene glycol block copolymer that is ~85%.