FI80711B - Foerfarande foer framstaellning av en terapeutiskt anvaendbar termiskt denaturerad, till en glykosidbindning ansluten antigen (gra), som haerroer sig fraon cancerceller. - Google Patents
Foerfarande foer framstaellning av en terapeutiskt anvaendbar termiskt denaturerad, till en glykosidbindning ansluten antigen (gra), som haerroer sig fraon cancerceller. Download PDFInfo
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- FI80711B FI80711B FI833652A FI833652A FI80711B FI 80711 B FI80711 B FI 80711B FI 833652 A FI833652 A FI 833652A FI 833652 A FI833652 A FI 833652A FI 80711 B FI80711 B FI 80711B
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0011—Cancer antigens
- A61K39/001102—Receptors, cell surface antigens or cell surface determinants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Claims (13)
1. Förfarande för framställning av en terapeutiskt använbar termiskt denaturerad, tili en glykosidbindning 5 ansluten antigen (GRA), som härrör sig frän cancerceller, där man separerar en cellmembrankomponent frän cancerceller med ett terminalt galaktos- och/eller terminalt N-acetylgalaktosaminbindande lektin för erhällande av en lektinreceptor, eller med en antikropp, som förmär bindas 10 vid en lektinreceptor, som erhällits frän en cancercell-membran och som kan bindas vid ett terminalt galaktos och/eller terminalt N-acetylgalkatosaminbindande lektin för erhällande av en fraktion, vilken har affinitet tili ett terminalt galaktos- och/eller terminalt N-acetylga-15 laktosaminbindande reagens, isolerar lektinreceptorn och/eller fraktionen för erhällande av en tili glykosidbindning ansluten antigen, kännetecknat där-av, att man upphettar den sälunda erhällna, tili en glykosidbindning anslutna antigenen. 20
2. Förfarande enligt patentkravet 1, känne tecknat därav, att den termiska denatureringen utförs genom att upphetta GRA vid c. 60-120eC, under 5 -60 minuter.
3. Förfarande enligt patentkravet 2, k ä n n e-25 tecknat därav, att GRA erhälls genom att fram- ställa cellmembrankomponenten frän cancercellerna medelst homogenisering eller upplösning, cellmembrankomponenten behandlas med lektin för bildande av ett lektincell-membrankomponentkomplex, det bildade komplexet uppsamlas, 30 lektinet avskiljs frän komplexet och den lektinfria mem-brankomponenten tillvaratas.
4. Förfarande enligt patentkravet 3, kännetecknat därav, att GRA är en cellmembrankomponent frän en human cancercell. 35
5. Förfarande enligt patentkravet 3, känne tecknat därav, att lektinet är jordnötslektin. 4i 8071 1
6. Förfarande enllgt patentkravet 3, känne-t e c k n a t därav, att lektlnet är Dolichos-bönans agglutinin.
7. Förfarande enligt patentkravet 2, känne- 5 tecknat därav, att GRA är en cellmembrankomponent frän en human cancercell.
8. Förfarande enligt patentkravet 2, känne-tecknat därav, att lektinet är jordnötslektin.
9. Förfarande enligt patentkravet 2, känne-10 tecknat därav, att lektinet är Dolichos-bönans agglutinin.
10. Förfarande enligt patentkravet 2, känne-tecknat därav, att GRA erhälls genom att fram-ställa cellmembrankomponenten frän cancercellerna medelst 15 homogenisering eller upplösning, cellmembrankomponenten behandlas med en GRA-antikropp för bildande av ett anti-kroppcellmembrankomponentkomplex, det bildade komplexet uppsamlas, antikroppen avskiljs frän komplexet och den antikroppfria membrankomponenten tillvaratas. 20
11. Förfarande enligt patentkravet 10, känne- tecknat därav, att GRA-antikroppen är en antikropp som har förmägan att bindas vid GRA, som är en cellmembrankomponent frän en cancercell, vilken komponent förmär bindas vid jordnötslektin och vilken erhälls med affini-25 tetskromatografi genom att använda ifrägavarande lektin.
12. Förfarande enligt patentkravet 10, känne-tecknat därav, att GRA-antikroppen är en antikropp frän en cancerpatient.
13. Förfarande enligt patentkravet 1, känne-30 tecknat därav, att man framställer en termiskt de- naturerad, tili en glykosidbindning ansluten antigen (termiskt denaturerad GRA), som härrör sig frän cancer-celler, vilken antigen innehäller ett glykoprotein, som bestär av en sackarid, som har terminalt galaktos och/ 35 eller N-acetylgalaktosamin och av termiskt denaturerat protein.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17741182 | 1982-10-08 | ||
| JP57177411A JPS5967224A (ja) | 1982-10-08 | 1982-10-08 | 糖鎖関連抗原およびその製造法ならびにこれを有効成分とする抗癌剤 |
| JP58100528A JPS59225119A (ja) | 1983-06-06 | 1983-06-06 | 熱変性抗原の製造法 |
| JP10052883 | 1983-06-06 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI833652A0 FI833652A0 (fi) | 1983-10-07 |
| FI833652A FI833652A (fi) | 1984-04-09 |
| FI80711B true FI80711B (fi) | 1990-03-30 |
| FI80711C FI80711C (sv) | 1990-07-10 |
Family
ID=26441535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI833652A FI80711C (sv) | 1982-10-08 | 1983-10-07 | Förfarande för framställning av en terapeutiskt användbar termiskt den aturerad, till en glykosidbindning ansluten antigen (GRA), som härrör sig från cancerceller |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0106285A3 (sv) |
| CA (1) | CA1236016A (sv) |
| DK (1) | DK463283A (sv) |
| ES (2) | ES526622A0 (sv) |
| FI (1) | FI80711C (sv) |
| NO (1) | NO833655L (sv) |
| PH (1) | PH23701A (sv) |
| PT (1) | PT77472B (sv) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60214737A (ja) * | 1984-04-06 | 1985-10-28 | Nippon Koutai Kenkyusho:Kk | 糖鎖関連抗原の製造法 |
| SE468231B (sv) * | 1984-05-18 | 1992-11-23 | Kabi Pharmacia Ab | Foerfarande foer att paavisa smaacelligt lungcarcinomantigen och anvaendning av antikroppar mot fukosylsialosylgangliotetraos foer framstaellning av komposition foer behandling eller in vivo/ diagnos |
| JPH03263401A (ja) * | 1990-03-13 | 1991-11-22 | Nippon Koutai Kenkyusho:Kk | 新規糖鎖 |
| MD838G2 (ro) * | 1992-07-21 | 1998-09-30 | Alexandr Nicolaenco | Remediu biologic activ cu proprietate imunomodulatoare, procedeu de obţinere a lui, preparat pe baza lui şi procedeu de normalizare a stării fiziologice a omului şi animalelor |
| US5376531A (en) * | 1992-09-03 | 1994-12-27 | Northwestern University | Method of detecting cancer |
| DK140992D0 (da) * | 1992-11-24 | 1992-11-24 | Ole Buchardt | Fremgangsmaade til frembringelse af antistoffer mod haptener og andre b-celle-antigener, antistoffer opnaaet ved fremgangsmaaden og anvendelse af disse antistoffer til fremstilling af vacciner, isaer til veterinaermedicinsk brug |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3152851A1 (de) * | 1981-05-19 | 1983-05-19 | Stephen Sugaar | Stark antigene tumorspezifische stoffe und verfahren zum nachweis von krebs unter verwendung dieser stoffe |
| GB2106935B (en) * | 1981-10-01 | 1985-01-30 | Otsuka Pharma Co Ltd | Cancer cell-combatting lymphocytes process for the production thereof and anticancer agents containing said lymphocytes |
| EP0087898A1 (en) * | 1982-02-22 | 1983-09-07 | Cancer Research Campaign Technology Limited | Antibodies and antigens useful in the diagnosis and treatment of cancer |
-
1983
- 1983-10-07 EP EP83110040A patent/EP0106285A3/en not_active Withdrawn
- 1983-10-07 CA CA000438680A patent/CA1236016A/en not_active Expired
- 1983-10-07 FI FI833652A patent/FI80711C/sv not_active IP Right Cessation
- 1983-10-07 PH PH29673A patent/PH23701A/en unknown
- 1983-10-07 PT PT77472A patent/PT77472B/pt not_active IP Right Cessation
- 1983-10-07 NO NO833655A patent/NO833655L/no unknown
- 1983-10-07 DK DK463283A patent/DK463283A/da not_active Application Discontinuation
- 1983-10-07 ES ES526622A patent/ES526622A0/es active Granted
-
1985
- 1985-05-30 ES ES544306A patent/ES8700056A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| PH23701A (en) | 1989-09-27 |
| PT77472A (en) | 1983-11-01 |
| ES8600692A1 (es) | 1985-11-01 |
| FI833652A (fi) | 1984-04-09 |
| FI80711C (sv) | 1990-07-10 |
| DK463283A (da) | 1984-04-09 |
| ES526622A0 (es) | 1985-11-01 |
| ES544306A0 (es) | 1986-10-16 |
| PT77472B (en) | 1986-02-12 |
| FI833652A0 (fi) | 1983-10-07 |
| EP0106285A3 (en) | 1987-09-02 |
| NO833655L (no) | 1984-04-09 |
| ES8700056A1 (es) | 1986-10-16 |
| DK463283D0 (da) | 1983-10-07 |
| CA1236016A (en) | 1988-05-03 |
| EP0106285A2 (en) | 1984-04-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM | Patent lapsed |
Owner name: OTSUKA PHARMACEUTICAL CO,_. LTD. |