CN217392429U - Chip for analyzing formed component - Google Patents

Abstract

A visible component analysis chip including an upper cover main body, an intermediate layer, and a substrate laminated in this order from top to bottom; an upper cover sample adding port and an upper cover detection window are arranged on the upper cover main body; a hollow runner outline structure is arranged on the middle layer; the flow channel outline structure comprises a middle layer sample injection port, a middle layer inlet part and a middle layer flattening part which are sequentially communicated; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the upper cover main body enters the flow channel, the flattening flow channel is communicated with the overflow flow channel to form a complete flow channel, the size of the outer edge of the upper cover main body is smaller than that of the outer edge of the substrate, and the upper surface of the substrate close to the outer edge is directly exposed outwards. The chip for analyzing a visible component can be provided which has the same focusing reference surface and can be formed by quickly settling the visible component in a sample-containing liquid mixture without stacking the visible component.

Description

Chip for analyzing formed component

Technical Field

The utility model relates to a there is chip for ingredient analysis, especially relates to bear the chip that contains the sample and mix the liquid for micro-imaging.

Background

In the prior art, the carrier for the specimen for microscopic imaging is typically a conventional slide. The glass slide is also provided with spaces for accommodating mixed liquid, and the spaces comprise open spaces and closed spaces containing cover glass. Generally, the cover glass is also a flat glass sheet, and no way is provided for the molding process of the space for accommodating the mixed liquid. If too big, also be not applicable to the occasion to tangible composition analysis with the help of the microscope in the space of holding mixed liquid, the space is too big, contains the sample mixed liquid and can produce the precipitation effect, and wherein tangible composition can pile up, is difficult to discern after the formation of image under the microscope. Similarly, the space is too small, the visible components cannot flow quickly and uniformly spread in the mixed solution containing the sample, and the number of the visible components is low, so that the accuracy of visible component analysis after microscopic imaging is also influenced.

In the prior art, a conventional slide glass is usually used for carrying a sample for microscopic imaging, and if the slide glass is covered, the top surface of the cover glass is usually used as a focusing reference surface of a microscope; both the dimensional error of the coverslip and the thickness of the solution layer cause the focusing reference plane of the microscope to float, adding to the complexity of the focusing process in the process of using automatic control for focusing.

The chip is used for carrying the sample-containing mixed liquid for microscopic imaging and analyzing the visible components in the sample-containing mixed liquid, the sample-containing mixed liquid needs to be quickly flattened to form a thin solution layer with moderate thickness and containing the visible components, and the visible components of the thin solution layer can be well tiled and displayed; various formed components can be changed from freely suspended in a thin solution layer to properly settled on a plane to facilitate microscopic imaging.

Disclosure of Invention

The to-be-solved technical problem of the utility model lies in avoiding among the above-mentioned prior art can't provide the suitable reference surface of focusing and neither producing tangible composition and piling up and can make tangible composition subside fast and form the weak point of the chip for tangible composition analysis of thin layer again, and provide one kind and have same reference surface of focusing, and contain tangible composition in the sample mixed liquid and not pile up and can subside the chip for tangible composition analysis that forms fast again.

The technical scheme for solving the technical problems is that the chip for analyzing the visible components is used for bearing and flatly paving a mixed solution containing a sample, so that the mixed solution containing the sample is suitable for obtaining a microscopic amplification digital image for analyzing the visible components; the chip for analyzing a visible component includes an upper cover main body, an intermediate layer and a substrate which are stacked in this order from top to bottom; an upper cover sample adding port and an upper cover detection window are arranged on the upper cover main body; a hollow runner outline structure is arranged on the middle layer; the flow channel outline structure comprises a middle layer sample adding port, a middle layer entering part and a middle layer flattening part which are sequentially communicated; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the position of the sample adding port of the upper cover corresponds to the position of the sample adding port of the middle layer; the position of the upper cover detection window corresponds to the position of the middle layer flat part; the bottom surface of the upper cover main body, the top surface of the substrate and the middle layer are enclosed to form an accommodating space, and the accommodating space is used for a flow channel for flowing and tiling a sample-containing mixed solution; the size of the outer edge of the upper cover main body is smaller than that of the outer edge of the substrate.

The lower bottom surface of the upper cover main body is also provided with an upper cover main body entering part and an upper cover main body flattening part; one end of the upper cover main body inlet part is communicated with the upper cover sample adding port; starting from the position corresponding to the sample adding port of the upper cover, extending the entering part of the upper cover main body obliquely downwards until the other end of the entering part of the upper cover main body is flush with the flat part of the upper cover main body; the vertical distance between the two ends of the entering part of the upper cover main body is less than or equal to 0.5 mm; the upper cover main body flat part is positioned at the center of the upper cover detection window, and the bottom surface of the upper cover main body flat part is flush with the lower bottom surface of the upper cover main body; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the inlet part of the upper cover main body is pressed above the inlet part of the middle layer and forms an inlet flow channel with the top surface of the substrate in an enclosing manner; the upper cover main body flat part is pressed above the middle layer flat part and forms a flat flow passage with the top surface of the substrate in a surrounding mode.

The lower bottom surface of the upper cover main body is also provided with an upper cover main body overflow part and an upper cover main body air hole; the flow passage outline structure also comprises an intermediate layer overflow part; the middle layer flat part is communicated with the middle layer overflow part; the position of the overflow part of the upper cover main body corresponds to the position of the overflow part of the middle layer; one end of the overflow part of the upper cover main body is flush with the flat part of the upper cover main body, and the overflow part of the upper cover main body extends upwards in an inclined way from the one end until the other end of the overflow part of the upper cover main body is communicated with the air hole of the upper cover main body; the vertical distance between the two ends of the overflow part of the upper cover main body is less than or equal to 0.5 mm; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the overflow part of the upper cover main body is pressed above the overflow part of the middle layer and forms an overflow flow channel with the top surface of the substrate in an enclosing manner; the position of the air hole of the upper cover main body corresponds to the tail end of the intermediate layer overflow part; the upper cover main body air hole is a through hole, and the upper cover main body air hole and the middle layer overflow part are communicated with the upper cover main body air hole and are used for being communicated with external air; the aperture of the air hole of the upper cover main body is less than or equal to 2 mm.

The upper cover detection window is a square sunken window; the top surface of the upper cover detection window is lower than the upper surface of the upper cover main body; the distance between the bottom surface of the upper cover detection window and the bottom surface of the substrate ranges from 0.01mm to 0.5 mm; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the bottom surface of the upper cover detection window is pressed above the middle layer flat part and forms a flat laying part of the flow channel with the surrounding of the top surface of the substrate.

The middle layer is made of adhesive films with viscosity, and the upper surface and the lower surface of the middle layer are both provided with viscosity; the upper cover main body and the middle layer are bonded through the upper surface of the middle layer; the lower surface of the intermediate layer is bonded to the substrate.

The flow passage outline structure is integrally hollowed; the middle layer inlet part and the middle layer overflow part are in a strip shape, and the middle layer flat part is square; the area of the middle layer flat part is smaller than that of the upper cover detection window.

The sample adding port of the upper cover is in an open horn shape; the upper part of the upper cover sample adding port is a hollow inverted round table, and the lower part of the upper cover sample adding port is a hollow cylinder; the upper cover main body is also provided with an upper cover main body boss; the upper cover main body boss extends from one end part of the upper cover main body to the center of the upper cover main body, and the upper surface of the upper cover main body boss is higher than that of the upper cover main body; the upper cover sample adding port is arranged on one side of the upper cover main body boss close to the center of the upper cover main body; the edge of the hollow inverted round table at the upper part of the upper cover sample adding port is flush with the upper surface of the upper cover main body boss.

The boss of the upper cover main body is also provided with a concave platform for grasping and positioning; the concave station sets up in the one side that upper cover main part boss is close to upper cover main part tip, and the bottom surface of concave station is less than the upper surface of upper cover main part boss.

The upper cover main body is also provided with at least three upper cover main body mounting positioning holes; wherein the two upper cover main body mounting positioning holes are symmetrically arranged at two sides of the upper cover main body boss; the other upper cover main body mounting positioning hole is arranged at the outer side close to the air hole of the upper cover main body, and the center of the upper cover sample adding port, the center of the air hole of the upper cover main body and the center of the upper cover main body mounting positioning hole are positioned on the longitudinal central line of the chip for forming component analysis; the middle layer is also provided with at least three middle layer mounting positioning holes, and the positions of the middle layer mounting positioning holes correspond to the positions of the upper cover main body mounting positioning holes one to one.

The upper cover main body is also provided with a direction indicating part, and the upper surface of the direction indicating part is higher than the upper surface of the upper cover main body; the direction indicating part is in an arrow shape, and the arrow direction of the direction indicating part is directed to one side edge of the upper cover main body; the upper cover body air hole and one upper cover body mounting positioning hole are located inside the arrow shape.

Compared with the prior art, one of the beneficial effects of the technical scheme in this application is that the outer border size of upper cover main part is less than the outer border size of base plate, the upper surface that the base plate is close to the outer border directly exposes to the outside, the base plate upper surface that exposes to the outside can be used as the reference surface that the microscope focuses, because the base plate upper surface is exactly the runner bottom surface, consequently focus based on such chip for the tangible composition analysis, avoid upper cover main part and intermediate level size and assembly size error's influence respectively, make the reference surface that the microscope focuses more have the uniformity. The automatic focusing device is very suitable for the occasions of large-scale application of automatic control focusing by using a machine, and the automatic focusing efficiency and the consistency of the focusing starting point are improved. The chip for analyzing the formed components is used for bearing and flatly paving the mixed solution containing the samples; subjecting the sample-containing mixture to a digital microscopic image acquisition for analysis of a formed component therein; the formed components in the sample-containing mixture settle on the upper surface of the substrate. The bottom surface of the upper cover detection window, the bottom surface of the substrate and the middle layer flat part are enclosed to form an accommodating space, and the accommodating space is used for flowing and tiling of a sample-containing mixed liquid. In the process of adjusting the focal length in microscopic imaging, a reference plane or a positioning plane is required; because the size of the outer edge of the upper cover main body is smaller than that of the outer edge of the substrate, the upper surface of the substrate close to the outer edge can be directly used as a positioning surface for microscopic imaging. Because the sample-containing mixed liquid flows and is flattened in the flow channel outline structure, the visible components in the sample-containing mixed liquid can be settled on the upper surface of the substrate, the substrate is made of glass or other smooth materials, and the flatness of the upper surface of the substrate is very ensured, so that the positioning surface of the microscopic imaging and the object which is microscopically amplified can be positioned by adopting the same reference. The interference caused by the high-low position deviation of the positioning surface to the microscopic imaging process when the upper surface of the upper cover main body is used as the positioning surface is avoided. The upper surface of the substrate close to the outer edge is directly used as a positioning surface for microscopic imaging, so that a stable reference positioning surface can be formed in the microscopic imaging focusing process, and the positioning reference surface is a reference surface for sedimentation of a microscopic imaging target.

Compared with the prior art, the technical scheme has the advantages that the vertical distance between the two ends of the entering part of the upper cover main body is smaller than or equal to 0.5 mm; the inlet flow channel is obliquely downward and enters the flattening flow channel with a slope; the sample-containing mixed liquid can be quickly flattened in the flow channel.

Compared with the prior art, the third beneficial effect of the technical scheme in the application is that the vertical distance between the two ends of the overflow part of the upper cover main body is less than or equal to 0.5 mm; the overflow runner is obliquely and upwards arranged with gradient, the tail end from the flattening runner to the overflow runner is an obliquely upward channel, and the air holes of the upper cover main body are communicated with the middle layer overflow part to provide a channel for discharging air in the runner. Therefore, the overflow speed is appropriate, the air in the flow channel can be smoothly and quickly discharged, and the sample-containing mixed liquid can be quickly flattened in the flow channel. The air hole of the upper cover main body also has the function of stopping liquid flow. The pore diameter of the air hole of the upper cover main body is less than or equal to 2 mm. The aperture size can play a role in intercepting flow, and can also balance the internal and external pressure of the flow channel just, so that the flow in the flow channel is smoother.

Compared with the prior art, the beneficial effect of the technical scheme in the application is that the range of the distance between the bottom surface of the upper cover detection window and the bottom surface of the substrate is 0.01mm to 0.5 mm; the bottom surface of the upper cover detection window, the bottom surface of the substrate and the middle layer flat part are enclosed to form an accommodating space, and the accommodating space is used for tiling containing sample mixed liquid. This size allows for rapid sedimentation without stacking of the formed components in the sample-containing mixture.

Compared with the prior art, the technical scheme has the beneficial effects that the middle layer is directly made of the adhesive film, so that an additional adhesive layer is omitted; the manufacturing process and materials are saved, the structure and the size of the flow channel can be controlled more accurately, and the accumulated size error of the multilayer bonding is avoided.

Compared with the prior art, the technical scheme has the beneficial effects that the open horn-shaped accommodating space gives a buffer space for the sample-containing mixed liquid before entering the flow channel, so that the flow of the sample-containing mixed liquid in the flow channel is facilitated; uncovered tubaeform accommodation space, when containing sample mixed liquid dropwise, the liquid level of entrance can be higher than the liquid level in the runner, makes to contain sample mixed liquid and spreads and tile at the middle level flat portion more easily.

Compared with the prior art, the detection window of the sinking upper cover is pressed above the middle layer flat part of the flow channel outline structure, so that the liquid level in the flow channel is also pressed down, and the liquid level in the flow channel corresponding to the middle layer entering part is higher than the liquid level in the flow channel corresponding to the middle layer flat part; under the action of the two liquid level differences, the solution can flow in the flow channel more quickly and can fill the whole flat part of the middle layer more quickly. The middle layer overflow part corresponds the runner and corresponds the runner UNICOM with middle layer flat portion, and the position of upper cover main part gas pocket corresponds the tail end of middle layer overflow part, makes the runner and the outside UNICOM of middle layer overflow part, is favorable to gaseous discharge in the runner, further accelerates the flow of solution in the runner, is full of whole middle layer flat portion more fast, also can eliminate the bubble in the middle layer flat portion simultaneously, has got rid of the influence of bubble to follow-up micro-imaging.

Compared with the prior art, the beneficial effects of the technical scheme in this application are that setting up of upper cover main part boss makes the upper cover sample addition mouth have bigger sample accommodation space, and the volume of the cavity of upper cover sample addition mouth upper portion inversion round platform can be bigger promptly, can hold abundant sample-containing mixed liquid in order to fill up the runner, and the volume of guaranteeing to contain the solution sample in the runner is sufficient to it can be full of by the sample-containing mixed liquid to guarantee middle level flat portion.

Drawings

FIG. 1 is a schematic top front projection view of an embodiment of a chip for analyzing a visible component;

FIG. 2 is a schematic view of an exploded status axonometric projection of an embodiment of a chip for analyzing a visible component;

FIG. 3 is a schematic diagram of an integrated status axonometric projection of an embodiment of a chip for analyzing a visible component;

FIG. 4 is a schematic perspective orthographic view of an embodiment of a chip for analyzing a visible component;

FIG. 5 is a schematic top front projection view of an embodiment of a chip for analyzing a visible component with a main body of a top cover removed;

FIG. 6 is a schematic bottom front view of an embodiment of a chip for analyzing a visible component;

FIG. 7 is a schematic cross-sectional AA view of FIG. 6;

FIG. 8 is an enlarged fragmentary view of FIG. 7, with the flat portion reduced in length as needed for clarity of illustration;

FIG. 9 is one of the schematic views of the upper cover body with its bottom facing upward;

fig. 10 is a perspective view schematically showing the upper cover main body with its bottom surface facing upward, in which reference numeral 213, i.e., an upper cover detection window 213, is located on the top surface of the upper cover main body.

Detailed Description

The following detailed description of embodiments of the present invention will be made with reference to the accompanying drawings.

In the embodiment of the chip for analyzing a tangible ingredient shown in fig. 1 to 3, the chip includes a top cover main body 200, an intermediate layer 500, and a substrate 600 stacked in this order from top to bottom; the upper cover body 200 is provided with an upper cover sample adding port 212 and an upper cover detection window 213; a hollow runner outline structure 510 is arranged on the middle layer 500; the flow channel outline structure 510 comprises a middle layer sample injection port 512, a middle layer entering part 513 and a middle layer flattening part 511 which are sequentially communicated;

when the upper cover main body 200, the middle layer 500 and the substrate 600 are sequentially stacked, the position of the upper cover sample adding port 212 corresponds to the position of the middle layer sample adding port 512; the position of the upper cover detection window 213 corresponds to the position of the middle layer flat part 511; forming a flow channel 700 for flowing a sample-containing mixture;

the outer edge of the upper cover body 200 is smaller than the outer edge of the substrate 600, and the upper surface 610 of the substrate close to the outer edge is directly exposed to the outside.

The sample-containing mixture is introduced from the top cover inlet 212, and sequentially enters the intermediate layer inlet 513, the intermediate layer flattening portion 511, and the intermediate layer overflow portion 514 through the intermediate layer inlet 512. The mixed solution containing the sample is diffused and flattened in the middle-layer flattening part 511 to form a thin solution layer, the thin solution layer is limited between the upper cover detection window 213 and the corresponding position of the substrate 600, and the thin solution layer can be seen from the outside through the upper cover detection window 213 and can be used for microscopic amplification of the mixed solution containing the sample. The size of the outer edge of the upper cover main body 200 is smaller than that of the outer edge of the substrate, the upper surface 610 of the substrate 600 close to the outer edge is directly exposed outwards, the upper surface of the substrate exposed outwards can be used as a reference surface for microscope focusing, and the upper surface 610 of the substrate is the bottom surface of the flow channel, so that focusing is performed based on the chip for visible component analysis, the influence of the respective size and assembly size errors of the upper cover main body and the middle layer is avoided, and the reference surface for microscope focusing is more consistent.

In the embodiment of the chip for analyzing a formed component shown in fig. 1 to 3, the upper cover main body 200 is further provided with an upper cover main body air hole 214; the flow channel profile structure 510 further comprises an intermediate layer overflow 514; the middle layer flattening part 511 is communicated with the middle layer overflow part 514 in sequence; the position of the upper cover body air hole 214 corresponds to the tail end of the middle layer overflow part 514; the lid body air holes 214 are through holes, and the lid body air holes 214 and the intermediate layer overflow 514 communicate with the lid body air holes for communication with the outside air. The upper cover main body air hole is communicated with the intermediate layer overflow part, and a channel is provided for air discharge in the flow passage. The air in the flow channel can be smoothly and quickly discharged, and the mixed liquid containing the sample can be quickly flattened in the flow channel.

In an embodiment of a chip for forming a component analysis shown in fig. 2 to 5, the intermediate layer 500 is made of an adhesive film having tackiness, and the upper and lower surfaces of the intermediate layer each have tackiness; the upper cover main body 200 and the middle layer 500 are bonded through the upper surface of the middle layer; the lower surface of the intermediate layer 500 is bonded to the substrate 600. The middle layer 500 is directly made of a sticky adhesive film, so that an additional adhesive layer is omitted; the manufacturing process and materials are saved, the structure and the size of the flow channel can be controlled more accurately, and the accumulated size error of the multilayer bonding is avoided. Of course, in some embodiments not shown in the drawings, the intermediate layer is provided with an upper adhesive layer and a lower adhesive layer; the upper cover main body and the middle layer are bonded through the upper bonding layer; the middle layer is adhered to the substrate through the lower adhesive layer.

In the embodiment of the chip for analyzing a formed component shown in FIGS. 1 to 5, the top lid sample addition port 212 has an open horn shape; the upper part of the upper cover sample adding port is a hollow inverted round table, and the lower part of the upper cover sample adding port is a hollow cylinder. The open horn-shaped containing space is used for providing a buffer space for the mixed liquid containing the sample before entering the flow channel, so that the flow of the mixed liquid containing the sample in the flow channel is facilitated; open horn shape accommodation space, when containing sample mixture dropwise, the liquid level of entrance can be higher than the liquid level in the runner, makes to contain the sample mixture and diffuses and tile in middle level flat portion more easily.

In the embodiment of the chip for analyzing a tangible component shown in fig. 1 to 4, the flow channel contour structure 510 is integrally hollow; the intermediate layer entry part 513 and the intermediate layer overflow part 514 are strip-shaped, and the intermediate layer flat part 511 is square; the area of the middle layer flat part 511 is smaller than that of the upper cover detection window 213; when the upper cover main body 200, the middle layer 500 and the substrate 600 are sequentially overlapped, the flow channel outline structure 510 is attached to the substrate 600, and the sunken upper cover detection window 513 is pressed above the middle layer flat part 511 of the flow channel outline structure 510; a flow channel is formed in the chip for analyzing a formed component.

In the embodiment of the chip for analyzing a formed component shown in fig. 6 to 8, the upper cover detection window is a sunken window, and the upper cover detection window is square; the top surface of the upper cover detection window is lower than the upper surface of the upper cover main body; the distance between the bottom surface of the upper cover detection window and the bottom surface of the substrate is 0.01-0.5 mm; the bottom surface of upper cover detection window, base plate bottom surface and the flat portion of intermediate level enclose and close and form an accommodation space, and this accommodation space is used for the tiling that contains the sample mixed liquid.

In the embodiment of the chip for analyzing a tangible ingredient shown in fig. 6 to 8, fig. 7 is a schematic cross-sectional view AA of fig. 6; fig. 8 is a partially enlarged view of fig. 7, in which the length of the middle flat portion 511 is reduced as needed for clarity. The cross-sectional view in fig. 8 shows a cross-sectional view of a complete flow channel 700; when the upper cover main body 200, the intermediate layer 500, and the substrate 600 are sequentially stacked; an inlet flow channel 710 is formed by the lower surface of the upper cover main body 200 and the part of the substrate 600 clamped and corresponding to the middle layer sample inlet 512 and the middle layer inlet 513; a flattening flow channel 720 is formed by the upper cover detection window 513 and the part of the substrate 600 clamped and corresponding to the middle layer flattening part 511; an overflow flow path 730 is formed at a portion corresponding to the intermediate layer overflow part 514 where the lower surface of the upper cover main body 200 and the substrate 600 are sandwiched; entering the flow channel 710, communicating the flattening flow channel 720 with the overflow flow channel 730 to form a complete flow channel 700, wherein the inlet of the flow channel 700 is the upper cover sample addition port 212; the outlet of the flow passage 700 is the cover body air hole 214; the flow channel 700 is integrally communicated, i.e., the upper cover sample inlet 212 is communicated with the upper cover main body air hole 214, so as to form the complete flow channel 700.

As shown in fig. 8 to 10, the lower bottom surface of the upper cover main body is further provided with an upper cover main body inlet 271, an upper cover main body flat part 272, and an upper cover main body overflow 273; one end of the upper cover main body inlet part 271 is communicated with the upper cover sample adding port 212; from the position corresponding to the upper cover sample addition port 212, the upper cover main body entering part 271 extends obliquely downwards until the other end of the upper cover main body entering part 271 is flush with the upper cover main body flat part 272; the vertical spacing between the ends of the cover body entry portion 271 is less than or equal to 0.5mm, and in some embodiments the vertical spacing between the ends of the cover body entry portion 271 is less than or equal to 0.2mm, such as 0.15 mm; the upper cover main body flat part 272 is located at the center of the upper cover detection window 213, and the bottom surface of the upper cover main body flat part 272 is flush with the lower bottom surface of the upper cover main body 200; the position of the upper cover body overflow part 273 corresponds to the position of the intermediate layer overflow part 514; one end of the upper cover body overflow part 273 is flush with the upper cover body flat part 272, and the upper cover body overflow part 273 extends obliquely upward from the one end until the other end of the upper cover body overflow part 273 is communicated with the upper cover body air hole 214; the vertical spacing between the ends of the cover body overflow 273 is less than or equal to 0.5mm, and in some embodiments the vertical spacing between the ends of the cover body overflow 273 is less than or equal to 0.2mm, such as 0.15 mm; when the upper cover main body 200, the middle layer 500 and the substrate 600 are sequentially overlapped, the upper cover main body entering part 271 is pressed above the middle layer entering part 513 and forms an entering flow channel 710 by enclosing with the top surface of the substrate 600; the cover body flat portion 272 is pressed on the middle layer flat portion 511 to form a flat flow channel 720. The upper cover main body overflow part 273 is pressed above the middle layer overflow part 514 and surrounds the top surface of the substrate to form an overflow flow channel 730; the position of the upper cover body air hole 214 corresponds to the tail end of the middle layer overflow part 514; the upper cover main body air hole is a through hole, and the upper cover main body air hole and the middle layer overflow part are communicated with the upper cover main body air hole and are used for being communicated with external air; the pore diameter of the air hole of the upper cover main body is less than or equal to 2 mm.

The vertical spacing between the two ends of the entry portion of the main body of the upper cover is less than or equal to 0.2mm, such as 0.15 mm; the inlet flow channel is obliquely downward and enters the flattening flow channel with a slope; the sample-containing mixed liquid can be quickly flattened in the flow channel. The vertical distance between the two ends of the overflow part of the upper cover main body is less than or equal to 0.2mm, such as 0.15 mm; the overflow runner is obliquely and upwards arranged with a slope, and a channel which is obliquely upwards is arranged from the flattening runner to the tail end of the overflow runner. After being added from the upper cover sample adding port 212, the sample-containing mixed solution firstly enters the inlet flow channel 710, and flows into the flattening flow channel 720 after being filled with the inlet flow channel 710 along the obliquely downward inlet flow channel 710; spread along the flattened channel 720 and fill and enter the overflow channel 730 until the overflow channel 730 is also filled. The inlet flow channel 710 and the overflow flow channel 730 are disposed at both sides of the flattening flow channel 720, and both the inlet flow channel 710 and the overflow flow channel 730 are disposed in an oblique direction, so that the center of gravity of the flattening flow channel 720 is the lowest. The flowing and flattening of the mixed liquid containing the sample are very convenient.

In the embodiment of the chip for analyzing a formed component shown in fig. 1 to 4, the upper cover main body is further provided with an upper cover main body boss 220; the upper cover main body boss 220 extends from one end of the upper cover main body to the center of the upper cover main body, and the upper surface of the upper cover main body boss 220 is higher than the upper surface of the upper cover main body 200; the upper cover sample adding port 212 is arranged on one side of the upper cover main body boss 220 close to the center of the upper cover main body; the edge of the hollow inverted round table at the upper part of the upper cover sample adding port 212 is flush with the upper surface of the upper cover main body boss.

Due to the arrangement of the bosses 220 of the upper cover main body, the upper cover sample adding port 212 has a larger sample accommodating space, namely the volume of the hollow inverted round table at the upper part of the upper cover sample adding port 212 can be larger, enough sample-containing mixed liquid can be accommodated to fill the flow channel, the amount of the sample containing solution in the flow channel is ensured to be enough, and the flat part of the middle layer can be ensured to be filled with the sample-containing mixed liquid.

In one embodiment of a chip for analyzing a visible component shown in fig. 1 to 4, the upper cover main body boss 220 is further provided with a recessed land 221 for grasping positioning; the concave stage 221 is provided on the side of the upper cover body boss 220 near the end of the upper cover body, and the bottom surface of the concave stage 221 is lower than the upper surface of the upper cover body boss 220.

The arrangement of the concave table facilitates taking the chip for analyzing the visible components, and the fixed position is used for grabbing, so that pollution of grabbing to other parts of the chip for analyzing the visible components is avoided, and the chip for analyzing the visible components is enabled to maintain a clean observation surface and a focus surface more easily.

In the embodiment of the chip for analyzing a formed component shown in fig. 1 to 4, the upper cover main body is further provided with at least three upper cover main body mounting positioning holes 260; two upper cover body mounting positioning holes (261,262) are symmetrically arranged at two sides of the upper cover body boss 220; another upper lid body fitting positioning hole 263 is provided on the outer side of the vicinity of the air vent of the upper lid body, and the center of the upper lid sample addition port 212, the center of the upper lid body air vent 214, and the center of the upper lid body fitting positioning hole 263 are located on the longitudinal center line of the chip for forming a component analysis;

the middle layer 500 is further provided with at least three middle layer mounting positioning holes 560, and the positions of the middle layer mounting positioning holes correspond to the positions of the upper cover body mounting positioning holes one to one. The upper cover body mounting positioning holes (261,262) correspond to two intermediate layer mounting positioning holes (561,562), and the other upper cover body mounting positioning hole 263 corresponds to the other intermediate layer mounting positioning hole 563; the positions of the upper cover main body mounting positioning holes and the intermediate layer mounting positioning holes correspond to each other one by one, and the positioning holes of each group form an isosceles triangle arrangement mode, so that the positioning between the upper cover main body and the intermediate layer can be accurately achieved. The center of the upper cover sample adding port, the center of the upper cover main body air hole and the center of the upper cover main body mounting positioning hole are on the same longitudinal central line, so that the positioning accuracy is further ensured.

In the embodiment of the chip for analyzing a formed component shown in fig. 1 to 4, the upper cover main body is further provided with a direction indicating portion, the direction indicating portion is in an arrow shape, and the arrow direction of the direction indicating portion is directed to one side edge of the upper cover main body; the upper cover body air hole and one upper cover body mounting positioning hole are located inside the arrow shape. The upper surface of the direction indicating part is higher than the upper surface of the upper cover main body; the direction indicating part indicates the flowing direction of the liquid in the flow channel, so that a user can observe and use the liquid conveniently, and the direction indicating part also indicates the direction of the chip for analyzing the formed components inserted into the host; the concave of the boss of the upper cover main body is used for being held by a user, and the direction indicating part prompts the insertion direction, so that the user can be ensured to insert the visible component analysis chip in the correct use method and direction. The upper surface of the direction indicating part is higher than the upper surface of the upper cover main body; more overflow spaces are formed in the air holes of the upper cover main body, so that mixed liquid and gas can flow out of the air holes of the upper cover main body conveniently.

A visible component analysis chip including an upper cover main body, an intermediate layer, and a substrate laminated in this order from top to bottom; an upper cover sample adding port and an upper cover detection window are arranged on the upper cover main body; a hollow runner outline structure is arranged on the middle layer; the flow channel outline structure comprises a middle layer sample adding port, a middle layer entering part and a middle layer flattening part which are sequentially communicated; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the upper cover main body enters the flow channel, the flattening flow channel is communicated with the overflow flow channel to form a complete flow channel, the size of the outer edge of the upper cover main body is smaller than that of the outer edge of the substrate, and the upper surface of the substrate close to the outer edge is directly exposed outwards. A chip for analyzing a formed component, which has the same focusing reference plane and is formed by quickly settling a formed component in a sample-containing mixture without stacking the formed component, can be provided.

As shown in fig. 1 to 10, the above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all the equivalent structures or equivalent processes implemented by the contents of the specification and the drawings are directly or indirectly applied to other related technical fields, and all the same principles are included in the scope of the present invention.

Claims (10)

1. A visible component analysis chip, comprising:

the device is used for bearing and spreading the mixed solution containing the sample, so that the mixed solution containing the sample is suitable for obtaining a microscopic magnification digital image for analyzing the visible components;

the chip for analyzing a visible component includes an upper cover main body, an intermediate layer and a substrate which are stacked in this order from top to bottom; an upper cover sample adding port and an upper cover detection window are arranged on the upper cover main body;

a hollow runner outline structure is arranged on the middle layer; the flow channel outline structure comprises a middle layer sample injection port, a middle layer inlet part and a middle layer flattening part which are sequentially communicated;

when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the position of the sample adding port of the upper cover corresponds to the position of the sample adding port of the middle layer; the position of the upper cover detection window corresponds to the position of the middle layer flat part; the bottom surface of the upper cover main body, the top surface of the substrate and the middle layer are enclosed to form an accommodating space, and the accommodating space is used for a flow channel for flowing and tiling a sample-containing mixed solution;

the size of the outer edge of the upper cover main body is smaller than that of the outer edge of the substrate.

2. The tangible composition analysis chip according to claim 1, wherein the chip further comprises a second chip for analyzing a second characteristic of the second chip,

the lower bottom surface of the upper cover main body is also provided with an upper cover main body entering part and an upper cover main body flattening part;

one end of the upper cover main body inlet part is communicated with the upper cover sample adding port; starting from the position corresponding to the sample adding port of the upper cover, extending the entering part of the upper cover main body obliquely downwards until the other end of the entering part of the upper cover main body is flush with the flat part of the upper cover main body; the vertical distance between the two ends of the entering part of the upper cover main body is less than or equal to 0.5 mm;

the upper cover main body flat part is positioned at the center of the upper cover detection window, and the bottom surface of the upper cover main body flat part is flush with the lower bottom surface of the upper cover main body;

when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the inlet part of the upper cover main body is pressed above the inlet part of the middle layer and forms an inlet flow channel with the top surface of the substrate in an enclosing manner; the upper cover main body flat part is pressed above the middle layer flat part and forms a flat flow passage with the top surface of the substrate in a surrounding mode.

3. The tangible composition analysis chip according to claim 2, wherein the chip further comprises a second chip for analyzing the second signal,

the lower bottom surface of the upper cover main body is also provided with an upper cover main body overflow part and an upper cover main body air hole;

the flow passage outline structure also comprises an intermediate layer overflow part; the middle layer flat part is communicated with the middle layer overflow part;

the position of the overflow part of the upper cover main body corresponds to the position of the overflow part of the middle layer; one end of the overflow part of the upper cover main body is flush with the flat part of the upper cover main body, and the overflow part of the upper cover main body extends upwards from the one end until the other end of the overflow part of the upper cover main body is communicated with the air hole of the upper cover main body;

the vertical distance between the two ends of the overflow part of the upper cover main body is less than or equal to 0.5 mm;

when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the overflow part of the upper cover main body is pressed above the overflow part of the middle layer and forms an overflow flow channel with the top surface of the substrate in an enclosing manner;

the position of the air hole of the upper cover main body corresponds to the tail end of the intermediate layer overflow part; the upper cover main body air hole is a through hole, and the upper cover main body air hole and the middle layer overflow part are communicated with the upper cover main body air hole and are used for being communicated with external air;

the pore diameter of the air hole of the upper cover main body is less than or equal to 2 mm.

4. The tangible composition analysis chip according to claim 1, wherein the chip further comprises a second chip for analyzing a second characteristic of the second chip,

the upper cover detection window is a square sunken window; the top surface of the upper cover detection window is lower than the upper surface of the upper cover main body;

the distance between the bottom surface of the upper cover detection window and the bottom surface of the substrate ranges from 0.01mm to 0.5 mm; when the upper cover main body, the middle layer and the substrate are sequentially overlapped, the bottom surface of the upper cover detection window is pressed above the middle layer flat part and forms a flat laying part of the flow channel with the surrounding of the top surface of the substrate.

5. The tangible composition analysis chip according to claim 1, wherein the chip further comprises a second chip for analyzing a second characteristic of the second chip,

the middle layer is made of adhesive films with viscosity, and the upper surface and the lower surface of the middle layer are both provided with viscosity;

the upper cover main body and the middle layer are bonded through the upper surface of the middle layer; the lower surface of the intermediate layer is bonded to the substrate.

6. The tangible composition analysis chip according to claim 1, wherein the chip is a chip for analyzing a tangible composition,

the flow passage outline structure is integrally hollowed; the middle layer inlet part and the middle layer overflow part are in a strip shape, and the middle layer flat part is square; the area of the middle layer flat part is smaller than that of the upper cover detection window.

7. The tangible composition analysis chip according to claim 1, wherein the chip further comprises a second chip for analyzing a second characteristic of the second chip,

the sample adding port of the upper cover is in an open horn shape; the upper part of the upper cover sample adding port is a hollow inverted round table, and the lower part of the upper cover sample adding port is a hollow cylinder; the upper cover main body is also provided with an upper cover main body boss; the upper cover main body boss extends from one end part of the upper cover main body to the center of the upper cover main body, and the upper surface of the upper cover main body boss is higher than that of the upper cover main body;

the upper cover sample adding port is arranged on one side of the upper cover main body boss close to the center of the upper cover main body; the edge of the hollow inverted round table at the upper part of the upper cover sample adding port is flush with the upper surface of the upper cover main body boss.

8. The tangible composition analysis chip according to claim 7, wherein the first chip is a chip for analyzing a visible light component,

the boss of the upper cover main body is also provided with a concave platform for grasping and positioning; the concave station sets up in the one side that upper cover main part boss is close to upper cover main part tip, and the bottom surface of concave station is less than the upper surface of upper cover main part boss.

9. The tangible composition analysis chip according to claim 7, wherein the first chip is a chip for analyzing a visible light component,

the upper cover main body is also provided with at least three upper cover main body mounting positioning holes; the two upper cover main body mounting positioning holes are symmetrically arranged on two sides of the upper cover main body boss; the other upper cover main body mounting positioning hole is arranged at the outer side close to the air hole of the upper cover main body, and the center of the upper cover sample adding port, the center of the air hole of the upper cover main body and the center of the upper cover main body mounting positioning hole are positioned on the longitudinal central line of the chip for forming component analysis;

the middle layer is also provided with at least three middle layer mounting positioning holes, and the positions of the middle layer mounting positioning holes correspond to the positions of the upper cover main body mounting positioning holes one to one.

10. The tangible composition analysis chip according to claim 3, wherein the chip further comprises a second chip for analyzing a second characteristic of the second chip,

the upper cover main body is also provided with a direction indicating part, and the upper surface of the direction indicating part is higher than the upper surface of the upper cover main body;

the direction indicating part is in an arrow shape, and the arrow direction of the direction indicating part is directed to one side edge of the upper cover main body; the upper cover body air hole and one upper cover body mounting positioning hole are located inside the arrow shape.

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