CN214503640U - Genital tract mycoplasma detection box - Google Patents
Genital tract mycoplasma detection box Download PDFInfo
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- CN214503640U CN214503640U CN202120624734.2U CN202120624734U CN214503640U CN 214503640 U CN214503640 U CN 214503640U CN 202120624734 U CN202120624734 U CN 202120624734U CN 214503640 U CN214503640 U CN 214503640U
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- cavity
- culture
- genital tract
- detection
- mycoplasma
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- 238000001514 detection method Methods 0.000 title claims abstract description 49
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 34
- 210000005000 reproductive tract Anatomy 0.000 title claims abstract description 24
- 238000012360 testing method Methods 0.000 claims abstract description 29
- 238000007789 sealing Methods 0.000 claims abstract description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000005192 partition Methods 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 11
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 11
- 229920000742 Cotton Polymers 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 19
- 241000204003 Mycoplasmatales Species 0.000 abstract description 15
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 241000204051 Mycoplasma genitalium Species 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010028470 Mycoplasma infections Diseases 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- HQOJMTATBXYHNR-UHFFFAOYSA-M thallium(I) acetate Chemical compound [Tl+].CC([O-])=O HQOJMTATBXYHNR-UHFFFAOYSA-M 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a can be convenient for detect genital tract mycoplasma to avoid genital tract mycoplasma to cultivate the in-process and receive the mycoplasma detection box of pollution. The genital tract mycoplasma detection box comprises a box body; a containing cavity and a culture cavity are arranged in the box body; a culture medium is arranged in the culture cavity; the upper end of the inner wall of the culture cavity is provided with a plurality of limiting bosses; one of the limiting bosses is provided with a slot; a detection test strip is arranged in the slot; a partition plate is arranged above the limiting boss; a sealing plate is arranged above the partition plate; a feeding nozzle is arranged on the sealing plate; a transparent cover is arranged on the feeding nozzle; a carbon dioxide generator is arranged on the transparent cover; a sealing film is arranged on the partition plate below the feeding nozzle; an observation window and a comparison card are arranged outside the culture cavity. By adopting the genital tract mycoplasma detection box, the detection time can be shortened, the detection is convenient, and meanwhile, the detection accuracy can be improved.
Description
Technical Field
The utility model belongs to the technical field of the medical treatment and specifically relates to a genital tract mycoplasma detects box.
Background
It is well known that: mycoplasma genitalium is the smallest genome of all Mycoplasma. The Mycoplasma genitalium is flask-shaped and has a rod-shaped structure at the end. Mycoplasma genitalium is the smallest genome of all Mycoplasma. The optimum growth temperature is 37 ℃. The growth was very slow, no growth was observed in the normal medium, liquid medium and aerobic conditions, and the growth was observed in SP-4 medium containing no thallium acetate. The mycoplasma genitalium can adhere to the surfaces of epithelial cells and red blood cells through an adhesion structure of the mycoplasma genitalium, can slide on the surfaces of the cells, and gradually enters the epithelial cells and the red blood cells to cause diseases.
The detection of the mycoplasma genitalium generally adopts culture detection, and the mycoplasma genitalium has extremely high requirements on a culture medium, grows slowly and is difficult to culture. Especially, the culture of the mycoplasma genitalium in the clinical specimen is not easy to successfully culture and detect, generally, the time is long, and the mycoplasma genitalium is easy to be polluted in the culture process; various methods are currently used to detect these mycoplasma in cell culture, including DNA fluorescent staining, mycoplasma culture, electron microscopy, and Polymerase Chain Reaction (PCR). At present, the domestic animal and biological product enterprises adopt a culture method for mycoplasma detection, the method can accurately detect mycoplasma viable bacteria and other various mycoplasma which most often cause cell pollution, and the detection period is about 4 weeks, so that the time consumption is long.
Uu and Mh are mainly detected by in vitro culture and are widely applied to clinical application. The mycoplasma genitalium has high nutritional requirements and does not grow in the common mycoplasma culture medium. Due to the great difficulty of culture, mycoplasma genitalium is mainly detected clinically by Polymerase Chain Reaction (PCR) technology. The mycoplasma culture is an important means for Uu and Mh detection in domestic medical institutions at present, and mainly uses a liquid culture medium for direct detection and simultaneously performs mycoplasma drug sensitivity test to achieve the aim of providing a basis for clinical treatment medication as soon as possible. However, the liquid culture method sometimes causes false positive due to bacterial or fungal contamination, and brings misdiagnosis to clinic. Solid culture is the direct microscopic observation of the colony morphology typical of Mycoplasma on the medium after 24-48h, which is the "gold standard" for the diagnosis of mycoplasma infections. However, the solid culture method has long detection time and is not favorable for clinical rapid diagnosis. Each method has advantages and disadvantages, and compared with other methods, the PCR method is relatively simple to operate and high in detection sensitivity, can detect mycoplasma pollution in early stage, and is the most common mycoplasma pollution detection method at present, so that the kit suitable for the PCR method has important research and development significance.
In the prior art, for example, Chinese patent application ZL200810093412.9 discloses a test strip for detecting mycoplasma in genital tract; can realize the detection of the mycoplasma in the genital tract.
Detection test paper based on above-mentioned genital tract mycoplasma the utility model relates to a detection box convenient to realize genital tract mycoplasma detection.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a can be convenient for detect genital tract mycoplasma to avoid genital tract mycoplasma to cultivate the in-process and receive the mycoplasma detection box in genital tract who pollutes.
The utility model provides a technical scheme that its technical problem adopted is: a genital tract mycoplasma detection box comprises a box body; a containing cavity and a culture cavity are arranged in the box body;
a culture medium is arranged in the culture cavity; the upper end of the inner wall of the culture cavity is provided with a plurality of limiting bosses; one of the limiting bosses is provided with a slot; a detection test strip is arranged in the slot;
a partition plate is arranged above the limiting boss; a sealing plate is arranged above the partition plate; a feeding nozzle is arranged on the sealing plate; a transparent cover is arranged on the feeding nozzle; a carbon dioxide generator is arranged on the transparent cover;
the carbon dioxide generator comprises a tablet storage barrel; the top of the sheet storage cylinder is provided with a rotary cover, and the bottom of the sheet storage cylinder is provided with an air hole;
the lower end of the sheet storage cylinder penetrates through the transparent cover to extend into the feeding nozzle and is in threaded fit with the transparent cover; a carbon dioxide generating piece is arranged in the piece storage barrel;
a sealing film is arranged on the partition plate below the feeding nozzle; a sampler containing cavity and a cotton swab test tube containing cavity are arranged in the containing cavity; a sliding inserting plate for sealing the containing cavity is arranged above the containing cavity;
a first observation window is arranged on one side of the culture cavity; a second observation window for observing the detection test strip is arranged at one end of the culture cavity; and a comparison card is arranged on one side of the second observation window.
Furthermore, a temperature display for displaying the temperature of the culture cavity is arranged on one side of the second observation window.
Furthermore, sealing flanges are arranged on the periphery of the partition plate.
Further, the transparent cover is in threaded fit with the feeding nozzle.
The utility model has the advantages that: the utility model discloses a genital tract mycoplasma detection box, because the culture cavity and the containing cavity are arranged in the box body; and a detection test strip is arranged in the culture cavity; in the process of cultivation, the detection of the genital tract mycoplasma in the cultivation process can be realized through the detection test paper strip, the detection time can be shortened, the detection is convenient, and meanwhile, the detection accuracy can be improved. Secondly, the storage box is arranged, so that the operation in the detection process is facilitated; the detection device is convenient to store. Finally, because the multiple sealing structures are arranged above the culture cavity, the culture medium can be prevented from being polluted in the culture process.
Drawings
FIG. 1 is an exploded view of an embodiment of the Mycoplasma genitalium testing cassette of the present invention;
FIG. 2 is a perspective view of the Mycoplasma genitalium detecting cassette according to the embodiment of the present invention;
FIG. 3 is a top view of the Mycoplasma genitalium testing cassette of the embodiment of the present invention;
FIG. 4 is a cross-sectional view A-A of FIG. 3;
the following are marked in the figure: 1-box body, 2-containing cavity, 3-culture cavity, 4-sliding inserting plate, 5-sealing plate, 6-clapboard, 8-culture medium, 9-limiting boss, 10-detection test strip, 11-feeding nozzle, 12-transparent cover, 13-storage barrel, 14-rotary cover, 15-air vent, 16-first observation window, 17-second observation window, 18-contrast card and 19-temperature display.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
As shown in fig. 1 to 4, the genital tract mycoplasma detecting box of the present invention comprises a box body 1; a containing cavity 2 and a culture cavity 3 are arranged in the box body 1;
a culture medium 8 is arranged in the culture cavity 3; a plurality of limiting bosses 9 are arranged at the upper end of the inner wall of the culture cavity 3; a slot 91 is arranged on one of the limiting bosses 9; a detection test strip 10 is arranged in the slot 91;
a partition plate 6 is arranged above the limiting boss 9; a sealing plate 5 is arranged above the partition plate 6; the sealing plate 5 is provided with a feeding nozzle 11; a transparent cover 12 is arranged on the feeding nozzle 11; a carbon dioxide generator is arranged on the transparent cover 12;
the carbon dioxide generator comprises a tablet storage barrel 13; the top of the sheet storage barrel 13 is provided with a screw cap 14, and the bottom of the sheet storage barrel is provided with an air hole 15;
the lower end of the tablet storage barrel 13 penetrates through the transparent cover 12 to extend into the feeding nozzle 11, and is in threaded fit with the transparent cover 12; a carbon dioxide generating piece is arranged in the tablet storage barrel 13;
a sealing film 62 is arranged on the partition plate 6 below the feeding nozzle 11; a sampler containing cavity 21 and a cotton swab test tube containing cavity 22 are arranged in the containing cavity 2; a sliding inserting plate 4 for sealing the accommodating cavity 2 is arranged above the accommodating cavity 2;
a first observation window 16 is arranged on one side of the culture cavity 3; a second observation window 17 for observing the detection test strip 10 is arranged at one end of the culture cavity 3; a comparison card 18 is arranged on one side of the second observation window 17.
In the using process, the sliding inserting plate 4 is opened to take out the test tube and the sampler; extracting a detection sample through a sampler; then by screwing the magazine drum 13; so that the magazine canister 13 is pushed open against the sealing membrane 62; then the sample is sent into the culture cavity 3 through the feed nozzle 11, and the feed nozzle is sealed through the transparent cover and the storage barrel 13; then placing a carbon dioxide generating piece in the piece storage cylinder 13, and screwing the screw cap 14; culturing the sample through a culture medium; after culturing for a period of time, observing the condition of the bacterial colony on the culture medium through the observation window, and detecting the color change of the test strip 10 and the bacterial colony on the test strip.
Specifically, the test strip 10 adopts ZL200810093412.9 to disclose a test strip for detecting mycoplasma genitalium.
In summary, the genital tract mycoplasma detection box of the utility model is provided with the culture cavity and the containing cavity in the box body; and a detection test strip is arranged in the culture cavity; in the process of cultivation, the detection of the genital tract mycoplasma in the cultivation process can be realized through the detection test paper strip, the detection time can be shortened, the detection is convenient, and meanwhile, the detection accuracy can be improved. Secondly, the storage box is arranged, so that the operation in the detection process is facilitated; the detection device is convenient to store. Finally, because the multiple sealing structures are arranged above the culture cavity, the culture medium can be prevented from being polluted in the culture process.
In order to observe the culture temperature, a temperature display 19 for displaying the temperature of the culture chamber 3 is further provided on one side of the second observation window 17.
In order to improve the sealing performance, a sealing flange 61 is further arranged on the periphery of the partition plate 6.
In order to facilitate the unscrewing of the transparent cover 12, said transparent cover 12 is further screw-fitted with the feed nozzle 11.
Claims (4)
1. A genital tract mycoplasma testing box is characterized in that: comprises a box body (1); a containing cavity (2) and a culture cavity (3) are arranged in the box body (1);
a culture medium (8) is arranged in the culture cavity (3); a plurality of limiting bosses (9) are arranged at the upper end of the inner wall of the culture cavity (3); a slot (91) is arranged on one limiting boss (9); a detection test strip (10) is arranged in the slot (91);
a partition plate (6) is arranged above the limiting boss (9); a sealing plate (5) is arranged above the partition plate (6); a feeding nozzle (11) is arranged on the sealing plate (5); a transparent cover (12) is arranged on the feeding nozzle (11); a carbon dioxide generator is arranged on the transparent cover (12);
the carbon dioxide generator comprises a tablet storage barrel (13); the top of the sheet storage cylinder (13) is provided with a screw cap (14), and the bottom is provided with an air hole (15);
the lower end of the tablet storage barrel (13) penetrates through the transparent cover (12) to extend into the feeding nozzle (11), and is in threaded fit with the transparent cover (12); a carbon dioxide generating piece is arranged in the tablet storage barrel (13);
a sealing film (62) is arranged on the partition plate (6) below the feeding nozzle (11); a sampler containing cavity (21) and a cotton swab test tube containing cavity (22) are arranged in the containing cavity (2); a sliding inserting plate (4) for sealing the accommodating cavity (2) is arranged above the accommodating cavity (2);
a first observation window (16) is arranged on one side of the culture cavity (3); a second observation window (17) for observing and detecting the test strip (10) is arranged at one end of the culture cavity (3); and a comparison card (18) is arranged on one side of the second observation window (17).
2. A genital tract mycoplasma test kit according to claim 1, wherein: and a temperature display (19) for displaying the temperature of the culture cavity (3) is arranged on one side of the second observation window (17).
3. A genital tract mycoplasma test kit according to claim 2, wherein: and sealing flanges (61) are arranged on the periphery of the partition plate (6).
4. A genital tract mycoplasma test kit according to claim 3, wherein: the transparent cover (12) is in threaded fit with the feeding nozzle (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120624734.2U CN214503640U (en) | 2021-03-26 | 2021-03-26 | Genital tract mycoplasma detection box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120624734.2U CN214503640U (en) | 2021-03-26 | 2021-03-26 | Genital tract mycoplasma detection box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214503640U true CN214503640U (en) | 2021-10-26 |
Family
ID=78200647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120624734.2U Active CN214503640U (en) | 2021-03-26 | 2021-03-26 | Genital tract mycoplasma detection box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214503640U (en) |
-
2021
- 2021-03-26 CN CN202120624734.2U patent/CN214503640U/en active Active
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