CN211179535U - Micro-droplet double-fluorescence signal detection device - Google Patents
Micro-droplet double-fluorescence signal detection device Download PDFInfo
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- CN211179535U CN211179535U CN201921954646.8U CN201921954646U CN211179535U CN 211179535 U CN211179535 U CN 211179535U CN 201921954646 U CN201921954646 U CN 201921954646U CN 211179535 U CN211179535 U CN 211179535U
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Abstract
The utility model provides a micro-droplet double-fluorescence signal detection device, which comprises a light combination module, a light source module and a light source module, wherein the light combination module is used for combining a first laser with a first wavelength and a second laser with a second wavelength into a mixed exciting light; the objective lens is positioned on a light conduction path of the mixed exciting light and is used for confocal the mixed exciting light on the micro liquid drop to be detected so as to excite and generate first fluorescence corresponding to the first laser and second fluorescence corresponding to the second laser; the light processing module comprises a first photomultiplier for acquiring first fluorescence, a second photomultiplier for acquiring second fluorescence, a first dichroic mirror for reflecting the mixed excitation light to the objective lens from the light combining module, and a second dichroic mirror for separating the first fluorescence from the second fluorescence. The utility model discloses a two fluorescence signal detection device of micro-droplet can acquire the two fluorescence detection signal of micro-droplet through closing optical module and light processing module to form the light processing framework that single aperture copolymerization was burnt, the testing result is abundanter, be suitable for scene and field wider, saves the experiment cost, improves detection work efficiency.
Description
Technical Field
The utility model relates to a micro-fluidic chip technical field, concretely relates to two fluorescence signal detection devices of micro-droplet.
Background
The biochip has wide application in new medicine development, disease diagnosis, gene expression analysis, etc. The technology of the micro-fluidic chip is mature day by day and becomes a focus of people. There are various biological and chemical processes in the microfluidic detection chip, and the processes are usually completed in the micro-scale flow channel space, wherein some devices capable of detecting the reaction process are also required. The existing detection means can be divided into CCD scanning and laser confocal scanning, the CCD scanning system has a simpler structure and a higher detection speed compared with the laser confocal scanning system, but has a lower transverse resolution, if the transverse resolution is required to be improved, the amplification factor of the imaging system needs to be improved, and the corresponding field of view is reduced (namely the area of a chip to be measured at one time is smaller), when the area of the chip to be measured is larger, the chip can be spliced after being measured in blocks for many times, and because the block scanning actually makes the chip and the imaging system move relatively in a mechanical movement mode, the mechanical positioning error forms the splicing error of a scanned image, so the method is not suitable for high-precision high-density biochip detection.
The biochip detection system constructed based on the laser confocal principle scans the biochip point by point, and the biochip is always positioned on a focal plane, so that the spot size of the exciting light is very small, and the transverse resolution is high. Because the laser confocal detection mode has the characteristics of high resolution and high sensitivity, clear digital fluorescence images and quantitative analysis results of antibodies and the like marked by fluorescence on the biochip can be obtained, and the method can become a new detection means mainly adopted by high-density biochip scanning.
SUMMERY OF THE UTILITY MODEL
Therefore, the to-be-solved technical problem of the utility model is to provide a two fluorescence signal detection device of micro-droplet can acquire the two fluorescence detection signals of micro-droplet through closing optical module and light processing module to form the light processing framework that single aperture copolymerization is burnt, the testing result is abundanter, suitable scene and field are wider, save the experiment cost, improve detection work efficiency.
In order to solve the above problem, the utility model provides a two fluorescence signal detection devices of micro-droplet, include:
the light combining module is used for combining first laser with a first wavelength and second laser with a second wavelength into mixed exciting light;
the objective lens is positioned on a light conduction path of the mixed exciting light and is used for confocal focusing the mixed exciting light on the micro-droplet to be detected so as to excite and generate first fluorescence corresponding to the first laser and second fluorescence corresponding to the second laser;
the light processing module comprises a first photomultiplier for acquiring first fluorescence, a second photomultiplier for acquiring second fluorescence, a first dichroic mirror for reflecting the mixed excitation light to the objective lens from the light combining module, and a second dichroic mirror for separating the first fluorescence from the second fluorescence.
Preferably, the light processing module further comprises a convex lens between the first dichroic mirror and the second dichroic mirror to refract the scattered light therethrough into parallel light.
Preferably, the light processing module further comprises a first color filter between the first photomultiplier tube and the second dichroic mirror, and/or a second color filter between the second photomultiplier tube and the second dichroic mirror.
Preferably, the light processing module further has a mounting housing, the mounting housing is configured with a light conducting channel, the light conducting channel has a first small hole corresponding to the first photomultiplier and a second small hole corresponding to the second photomultiplier, and the first small hole and the second small hole are arranged in a conjugate manner.
Preferably, the mounting housing includes a fixing member, a first connecting member, a second connecting member, and a third connecting member, the fixing member has a first light ray injection opening, and a second light ray injection opening, the first light ray injection opening is coaxial with the first light ray injection opening, the second light ray injection opening is perpendicular to the first light ray injection opening, the second connecting member is connected to the first light ray injection opening, and the convex lens is sandwiched between the first light ray injection opening and the second light ray injection opening; the third connecting piece is connected to the first light ray emitting opening, the second dichroic mirror is clamped between the first connecting piece and the first light ray emitting opening, a first photomultiplier fixing seat is arranged at one end, away from the fixing piece, of the third connecting piece, and the first color filter and the first small hole piece are sequentially clamped between the third connecting piece and the first photomultiplier fixing seat along a light ray conducting path; the second light ray outlet is connected with a second photomultiplier fixing seat, and a second color filter and a second small hole piece are sequentially clamped between the third connecting piece and the second photomultiplier fixing seat along a light ray conducting path; the first connecting piece is connected to one side, far away from the fixing piece, of the second connecting piece, and the first dichroic mirror is clamped between the first connecting piece and the second connecting piece.
Preferably, a joint between the first connecting piece and the second connecting piece, and/or a joint between the second connecting piece and the fixing piece, and/or a joint between the third connecting piece and the fixing piece, and/or a joint between the first photomultiplier tube fixing seat and the third connecting piece, and/or a joint between the second photomultiplier tube fixing seat and the fixing piece is provided with a sealing shock absorption piece.
Preferably, the objective lens is movably connected to a side of the first connecting piece, which is away from the second connecting piece.
Preferably, the light combining module includes a first laser, a second laser, a reflective mirror and a third dichroic mirror, and the first laser light emitted by the first laser is reflected by the reflective mirror and then combined with the second laser light emitted by the second laser into the mixed excitation light at the third dichroic mirror.
Preferably, the light combining module further includes a fixing plate and a light combining cassette, the first laser, the second laser and the light combining cassette are fixedly connected to the fixing plate, the reflective mirror and the third dichroic mirror are disposed in the light combining cassette, and the light combining cassette has a mixed excitation light exit port.
Preferably, the first laser is a 532nm laser; and/or the second laser is a 473nm laser.
The utility model provides a pair of two fluorescence signal detection device of micro-droplet, first laser and the second laser that are different through the wavelength are in the synthesis formation treat behind the mixed excitation light that detect micro-droplet arouses and forms first fluorescence and second fluorescence, and pass through first photomultiplier and second photomultiplier reach after acquireing respectively and carry out corresponding processing (for example count) to the data processing equipment (such as computer etc.) that corresponds to realized that the single detects and to acquire the multinomial detection parameter, and formed the light processing framework that single aperture copolymerization is burnt, made the testing result abundanter, it is more extensive to be suitable for scene and field, this reduction experiment cost that can very big degree improves and detects work efficiency.
Drawings
Fig. 1 is a schematic view of an exploded structure of a micro-droplet dual fluorescence signal detection device according to an embodiment of the present invention;
fig. 2 is a schematic diagram of a light transmission path of the micro-droplet dual fluorescence signal detection device according to an embodiment of the present invention;
FIG. 3 is an exploded view of the optical processing module of FIG. 1;
fig. 4 is an exploded structural diagram of the light combining module in fig. 1.
The reference signs are:
1. a light combining module; 11. a first laser; 12. a second laser; 13. a fixing plate; 14. a light-combining cassette; 141. a reflective mirror; 142. a third dichroic mirror; 2. an objective lens; 3. a light processing module; 31. a first photomultiplier tube; 32. a second photomultiplier tube; 33. a first dichroic mirror; 34. a second dichroic mirror; 35. a first color filter; 36. a second color filter; 371. a fixing member; 372. a first connecting member; 373. a second connecting member; 374. a third connecting member; 375. a first photomultiplier tube holder; 376. a first orifice member; 377. a second photomultiplier tube holder; 378. a second orifice member; 38. a convex lens; 391. a shock pad; 392. a gasket; 393. a light-tight pad; 100. micro-droplets are detected.
Detailed Description
Referring to fig. 1 to 4 in combination, according to an embodiment of the present invention, there is provided a micro-droplet dual fluorescence signal detection apparatus, including: the light combining module 1 is used for combining first laser with a first wavelength and second laser with a second wavelength into mixed excitation light; the objective lens 2 is located on a light conduction path of the mixed excitation light, and is used for confocal focusing the mixed excitation light on the microdroplet 100 to be detected so as to excite and generate first fluorescence corresponding to the first laser and second fluorescence corresponding to the second laser; the light processing module 3 includes a first photomultiplier tube (PMT)31 for acquiring the first fluorescence, a second PMT 32 for acquiring the second fluorescence, a first dichroic mirror 33 for reflecting the mixed excitation light from the light combining module to the objective lens 2, and a second dichroic mirror 34 for separating the first fluorescence from the second fluorescence. In the technical scheme, the mixed excitation light is synthesized by the first laser and the second laser with different wavelengths to excite the micro-droplet 100 to be detected and form the first fluorescence and the second fluorescence, and the obtained mixed excitation light is respectively transmitted to the corresponding data processing equipment (such as a computer) through the first photomultiplier tube 31 and the second photomultiplier tube 32 to be correspondingly processed (such as counting), so that multiple detection parameters can be obtained by single detection, a single-pore confocal light processing framework is formed, the detection result is richer, the application scenes and the application fields are wider, the experiment cost can be greatly reduced, and the detection work efficiency is improved. It is worth mentioning that, because the detection device of the present application forms a single-aperture confocal light processing architecture, the light path is simpler, the light path is shorter, the energy of the focused light is more concentrated, the number of structural members is less, and fewer optical lenses can be adopted, so that the production and manufacturing of the device are reduced.
Preferably, the light processing module 3 further includes a convex lens 38, and the convex lens 38 is located between the first dichroic mirror 33 and the second dichroic mirror 34, so that the scattered light passing through the convex lens 38 is refracted into parallel light, so that the light passing through the convex lens 38 can be emitted to the second dichroic mirror 34 more regularly, and the detection accuracy of the subsequent fluorescent signal is ensured.
Further, the light processing module 3 further includes a first color filter 35, the first color filter 35 being located between the first photomultiplier tube 31 and the second dichroic mirror 34, and/or the light processing module 3 further includes a second color filter 36, the second color filter 36 being located between the second photomultiplier tube 32 and the second dichroic mirror 34.
Specifically, the light processing module 3 further has a mounting housing, the mounting housing is configured with a light conduction channel, the light conduction channel has a first small hole corresponding to the first photomultiplier tube 31 and a second small hole corresponding to the second photomultiplier tube 32, and the first small hole and the second small hole are arranged in a conjugate manner. More specifically, the mounting housing includes a fixing member 371, a first connecting member 372, a second connecting member 373, and a third connecting member 374, the fixing member 371 has a first light injecting opening, and a second light injecting opening, the first light injecting opening is coaxial with the first light injecting opening, the second light injecting opening is perpendicular to the first light injecting opening, the second connecting member 373 is connected to the first light injecting opening, and the convex lens 38 is sandwiched between the first light injecting opening and the second light injecting opening; the third connecting member 374 is connected to the first light emitting port, and the second dichroic mirror 34 is sandwiched therebetween, a first photomultiplier tube holder 375 is disposed at an end of the third connecting member 374 away from the fixing member 371, and the first color filter 35 and a first small hole member 376 (on which the first small hole is configured) are sequentially sandwiched between the third connecting member 374 and the first photomultiplier tube holder 375 along a light conducting path; the second light emitting opening is connected to a second photomultiplier tube holder 377, and the second color filter 36 and a second small hole member 378 (on which the first small hole is configured) are sequentially interposed between the third connector 374 and the second photomultiplier tube holder 377 along a light conducting path; the first connecting member 372 is connected to a side of the second connecting member 373 away from the fixing member 371, and the first dichroic mirror 33 is sandwiched between the first connecting member and the second connecting member. In this technical solution, a specific implementation manner of the light processing module 3 is provided, which is formed by mutually assembling and assembling each relatively independent connector and components, and it can be understood that, in order to ensure the rationality of the light conducting channel, in particular, the design of the light conducting channel conforms to the light processing path shown in fig. 2 of the present application, that is, although the foregoing does not specifically define the corresponding light conducting channels in the first connector 372, the second connector 373, and the third connector 374, it is clear that the first connector 372, the second connector 373, the fixing member 371, and the third connector 374 are sequentially located on the light conducting path and satisfy the light conducting requirement shown in fig. 2, and in order to ensure the light conducting requirement of fig. 2 and the compactness of the overall structure of the light processing module 3, the first connector 372, the second connector 373, and the third connector 374 are all isosceles right triangles in shape, that is, they all have a 45 ° slope, and the fixing element 371 also has a 45 ° slope.
Further, in order to protect the optical lens such as the convex lens 38 disposed in the light processing module 3 from damage and ensure that the optical lens is protected from external light, it is preferable that the connection between the first connection member 372 and the second connection member 373, and/or the connection between the second connection member 373 and the fixing member 371, and/or the connection between the third connection member 374 and the fixing member 371, and/or the connection between the first photomultiplier tube fixing base 375 and the third connection member 374, and/or the connection between the second photomultiplier tube fixing base 377 and the fixing member 371 is provided with a sealing shock absorber, which may include one or more of a shock absorbing pad 391, a sealing pad 392, and a sealing pad 393, for example.
The objective lens 2 is movably connected to a side of the first connecting member 372 away from the second connecting member 373 as a component for performing focus detection on the microdroplets 100 to be detected, as a specific embodiment, it is only required to use a 20-fold microscope, and it can be understood that the movable connection here means that the objective lens 2 has a rotational (around Z axis) degree of freedom and a translational (along Z axis or X axis) degree of freedom, so as to ensure that a detection person can flexibly adjust a focal point (focal plane) of the objective lens 2, and further, it can be understood that the aforementioned movement of the objective lens 2 can be controlled by a power module such as a lead screw motor.
As one specific embodiment of the light combining module 1, preferably, the light combining module 1 includes a first laser 11, a second laser 12, a reflective mirror 141, and a third dichroic mirror 142, and the first laser light emitted by the first laser 11 is reflected by the reflective mirror 141 and then combined with the second laser light emitted by the second laser 12 into the mixed excitation light by the third dichroic mirror 142. For example, the first laser 11 is a 532nm laser, in which case it is a green laser; and/or the second laser 12 is a 473nm laser, in this case a blue laser. More specifically, the light combining module 1 further includes a fixing plate 13 and a light combining cassette 14, the first laser 11, the second laser 12 and the light combining cassette 14 are fixedly connected to the fixing plate 13, the reflective mirror 141 and the third dichroic mirror 142 are disposed in the light combining cassette 14, and the light combining cassette 14 has a mixed excitation light exit. At this time, it can be understood that the light combining module 1 is constructed as a whole, so that the micro-droplet dual fluorescence signal detection apparatus is further compact in structure.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A micro-droplet dual fluorescence signal detection device, comprising:
the light combining module (1) is used for combining first laser with a first wavelength and second laser with a second wavelength into mixed excitation light;
the objective lens (2) is positioned on a light conduction path of the mixed exciting light and is used for focusing the mixed exciting light on the micro-droplet (100) to be detected so as to excite and generate first fluorescence corresponding to the first laser and second fluorescence corresponding to the second laser;
the light processing module (3) comprises a first photomultiplier (31) for acquiring first fluorescence, a second photomultiplier (32) for acquiring second fluorescence, a first dichroic mirror (33) for reflecting the mixed excitation light to the objective lens (2) by the light combining module, and a second dichroic mirror (34) for separating the first fluorescence from the second fluorescence.
2. The detection apparatus according to claim 1, wherein the light processing module (3) further comprises a convex lens (38), the convex lens (38) being between the first dichroic mirror (33) and the second dichroic mirror (34) to refract the scattered light therethrough into parallel light.
3. The detection apparatus according to claim 2, wherein the light processing module (3) further comprises a first color filter (35), the first color filter (35) being between the first photomultiplier tube (31) and the second dichroic mirror (34), and/or wherein the light processing module (3) further comprises a second color filter (36), the second color filter (36) being between the second photomultiplier tube (32) and the second dichroic mirror (34).
4. A detection device according to claim 3, characterized in that the light processing module (3) further has a mounting housing configured with a light conducting channel having a first aperture corresponding to the first photomultiplier tube (31) and a second aperture corresponding to the second photomultiplier tube (32), the first aperture being arranged in conjugate with the second aperture.
5. The detecting device according to claim 4, wherein the mounting housing includes a fixing member (371), a first connecting member (372), a second connecting member (373), and a third connecting member (374), the fixing member (371) has a first light injecting opening, and a second light injecting opening, the first light injecting opening is coaxial with the first light injecting opening, the second light injecting opening is perpendicular to the first light injecting opening, the second connecting member (373) is connected to the first light injecting opening, and the convex lens (38) is sandwiched therebetween; the third connecting piece (374) is connected to the first light emitting opening, the second dichroic mirror (34) is clamped between the third connecting piece and the first light emitting opening, a first photomultiplier fixing seat (375) is arranged at one end, away from the fixing piece (371), of the third connecting piece (374), and the first color filter (35) and the first small hole piece (376) are sequentially clamped between the third connecting piece (374) and the first photomultiplier fixing seat (375) along a light conducting path; the second light ray outlet is connected with a second photomultiplier fixing seat (377), and a second color filter (36) and a second small hole piece (378) are sequentially clamped between the third connecting piece (374) and the second photomultiplier fixing seat (377) along a light ray conducting path; the first connecting piece (372) is connected to one side, away from the fixing piece (371), of the second connecting piece (373), and the first dichroic mirror (33) is clamped between the first connecting piece and the second connecting piece.
6. The detection apparatus according to claim 5, wherein a junction between the first connector (372) and the second connector (373), and/or a junction between the second connector (373) and the fixture (371), and/or a junction between the third connector (374) and the fixture (371), and/or a junction between the first photomultiplier tube holder (375) and the third connector (374), and/or a junction between the second photomultiplier tube holder (377) and the fixture (371) is provided with a sealing shock absorber.
7. The detection apparatus according to claim 5, wherein the objective lens (2) is movably connected to a side of the first connection member (372) facing away from the second connection member (373).
8. The detection device according to any one of claims 1 to 7, wherein the light combining module (1) comprises a first laser (11), a second laser (12), a reflective mirror (141), and a third dichroic mirror (142), and the first laser light emitted from the first laser (11) is reflected by the reflective mirror (141) and combined with the second laser light emitted from the second laser (12) into the mixed excitation light at the third dichroic mirror (142).
9. The detecting device according to claim 8, wherein the light combining module (1) further comprises a fixing plate (13) and a light combining cassette (14), the first laser (11), the second laser (12) and the light combining cassette (14) are fixedly connected to the fixing plate (13), the reflective mirror (141) and the third dichroic mirror (142) are disposed in the light combining cassette (14), and the light combining cassette (14) has a mixed excitation light exit.
10. Detection apparatus according to claim 8, characterized in that the first laser (11) is a 532nm laser; and/or the second laser (12) is a 473nm laser.
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| CN201921954646.8U CN211179535U (en) | 2019-11-13 | 2019-11-13 | Micro-droplet double-fluorescence signal detection device |
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| CN201921954646.8U CN211179535U (en) | 2019-11-13 | 2019-11-13 | Micro-droplet double-fluorescence signal detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702657A (en) * | 2019-11-13 | 2020-01-17 | 新羿制造科技（北京）有限公司 | Micro-droplet double-fluorescence signal detection device |
| CN111896516A (en) * | 2020-09-07 | 2020-11-06 | 新羿制造科技（北京）有限公司 | Microdroplet Dual Fluorescence Signal Detection Device |
-
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702657A (en) * | 2019-11-13 | 2020-01-17 | 新羿制造科技（北京）有限公司 | Micro-droplet double-fluorescence signal detection device |
| CN111896516A (en) * | 2020-09-07 | 2020-11-06 | 新羿制造科技（北京）有限公司 | Microdroplet Dual Fluorescence Signal Detection Device |
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