CN117050888B - A donkey-hide gelatin composite polypeptide suitable for postpartum recovery and preparation method thereof - Google Patents
A donkey-hide gelatin composite polypeptide suitable for postpartum recovery and preparation method thereof Download PDFInfo
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- CN117050888B CN117050888B CN202311293889.2A CN202311293889A CN117050888B CN 117050888 B CN117050888 B CN 117050888B CN 202311293889 A CN202311293889 A CN 202311293889A CN 117050888 B CN117050888 B CN 117050888B
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Abstract
The invention belongs to the technical field of donkey-hide gelatin polypeptides, and particularly relates to a compound microbial inoculum and application thereof in preparation of donkey-hide gelatin polypeptides and donkey-hide gelatin paste by fermenting donkey-hide gelatin. The invention uses the compound microbial inoculum composed of trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007 to carry out specific co-fermentation on traditional Chinese medicine and high-quality donkey-hide gelatin, the donkey-hide gelatin is decomposed into donkey-hide gelatin polypeptide in the fermentation process, and various active ingredients in the traditional Chinese medicine are introduced while the donkey-hide gelatin is decomposed into the donkey-hide gelatin polypeptide, thus forming the donkey-hide gelatin compound polypeptide. The method has high donkey-hide gelatin hydrolysis rate, and unexpected effect of promoting milk secretion due to the addition of traditional Chinese medicine. The donkey-hide gelatin composite polypeptide prepared by the invention has great development prospect in the field of postpartum recovery, and can be used for preparing donkey-hide gelatin polypeptide paste and the like.
Description
Technical Field
The invention belongs to the technical field of donkey-hide gelatin polypeptides, and particularly relates to a compound microbial inoculum and application thereof in preparation of donkey-hide gelatin polypeptides and donkey-hide gelatin paste by fermenting donkey-hide gelatin.
Background
Donkey-hide gelatin is a traditional Chinese medicinal material and has many effects such as replenishing blood, stopping bleeding, nourishing yin, moistening dryness and the like. Donkey-hide gelatin has remarkable curative effect and is praised as a non-material cultural heritage in China over 2000. At present, donkey-hide gelatin has more researches and has more intensive researches on pharmacological actions and substance bases of related actions. Donkey-hide gelatin has rich protein content, and is difficult to achieve in vivo efficacy for patients with indigestion. Therefore, the donkey-hide gelatin needs to be subjected to enzymolysis to obtain small-molecule donkey-hide gelatin polynnaeus. Researches prove that the polypeptide has an antioxidant function and is a natural antioxidant.
Donkey-hide gelatin is prepared from donkey skin of equine animals by water decoction and concentration, and thus has a complex and high content of chemical components. The main component consists of 18 amino acids and 20 microelements and is collagen hydrolysate with small molecular weight. The protein content is rich and reaches about 80 percent. 18. The amino acids are Lys (2.63%), his (0.59%)
Ile (0.91%), arg (4.42%), thr (3.21%), met (0.20%), glu (0.59%), pro (6.52%), gly (13.36%), ala (0.12%), and the like. 8 human essential amino acids are found in donkey-hide gelatin, wherein the amino acids account for 16.98-21.22% of the total amino acid content. Donkey-hide gelatin also contains various trace metal elements such as K, na, ca, mg, fe, mn, zn, etc., 8 of which are 4 necessary for human body. Colla Corii Asini has hemostatic, antifatigue, tumor growth inhibiting, immunity enhancing, and gynecological diseases improving effects.
The invention aims to further expand the application field of donkey-hide gelatin, in particular to the postpartum nourishing field.
Disclosure of Invention
The probiotics are an important direction of traditional Chinese medicine modernization, and the invention team also carries out a great deal of research on the probiotics, and the preparation process of the compound donkey-hide gelatin peptide which can prepare postpartum nourishing, especially promote milk secretion is unexpectedly discovered.
Based on the reasons, the invention provides a composite microbial inoculum for fermenting donkey-hide gelatin, which is characterized by consisting of trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007.
In addition, the method for producing the donkey-hide gelatin composite polypeptide suitable for postpartum recovery by using the composite microbial inoculum is provided, and the main operation steps are as follows:
S1, preprocessing donkey-hide gelatin, namely selecting high-quality donkey-hide gelatin as a raw material, ensuring the purity and quality of the donkey-hide gelatin, and crushing the donkey-hide gelatin into 400-500-mesh particles for later use;
s2, preparing Trichoderma CGMCC 3.3711 (purchased from Shanghai collection biotechnology center) bacterial liquid, namely preparing Trichoderma CGMCC 3.3711 bacterial liquid with the concentration of 1.5X10 9~3×109 cfu/ml, preparing yeast CMCC (B) 98007 (purchased from Beijing Bai European Bo Wei biotechnology Co., ltd.) bacterial liquid, namely preparing yeast CMCC (B) 98007 bacterial liquid with the concentration of 3.5X10 9~4.6×109 cfu/ml for later use;
S3, pretreatment of rhizoma polygonati, chinese angelica, medlar, dendrobium officinale flower, kudzuvine root and ginger, wherein the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium officinale flower, the kudzuvine root and the ginger are crushed according to the same mass ratio and then are uniformly mixed with water according to the mass ratio of 1:1.5, then the mixture is added with trichoderma liquid and saccharomycete liquid prepared in the step S2 for jar fermentation, wherein the traditional Chinese medicine mixture comprises the trichoderma liquid and saccharomycete liquid according to the mass ratio of 1-4:0.5-2:0.5-2, the fermentation temperature is 32-35 ℃ and the fermentation time is 12-24 hours;
s4, filtering after fermentation is completed, taking supernatant fluid after filtration, adding the donkey-hide gelatin crushed in the step S1, stirring uniformly at the temperature of 32-35 ℃, and then keeping the temperature of 32-35 ℃ for fermentation for 6-12 hours to obtain donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, wherein the fermentation liquor is added according to the mass ratio of 1:1;
S5, after fermentation is completed, adding 3%H 2O2 solution to inactivate trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007, filtering donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, removing solid particles and impurities, adding 2mM NaOH to promote precipitation of donkey-hide gelatin polypeptide, centrifuging, completing centrifugation, collecting precipitate, and drying the collected precipitate at 30-40 ℃ to obtain the donkey-hide gelatin composite polypeptide for postpartum recovery.
Preferably, in the step S1, high-quality donkey-hide gelatin is selected.
Preferably, the concentration of the trichoderma CGMCC 3.3711 bacterial liquid used in the step S2 is 2.2X10 9 cfu/ml.
Preferably, the concentration of the yeast CMCC (B) 98007 used in the step S2 of the present invention is 3.9X10 9 cfu/ml.
Preferably, the step S3 of crushing the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium officinale flower, the kudzuvine root and the ginger according to the same mass ratio and uniformly mixing.
Preferably, in the step S3, the traditional Chinese medicine mixture, namely trichoderma liquid and saccharomycete liquid are uniformly mixed according to the mass ratio of 1:0.5:0.5.
Preferably, the supernatant in the step S4 is not subjected to fire extinguishment, and is directly mixed with the melted donkey-hide gelatin in the step S1 for fermentation.
Preferably, the donkey-hide gelatin composite polypeptide obtained in the step S5 is used for preparing donkey-hide gelatin composite polypeptide paste;
the preparation method comprises the following steps:
Heating high-quality donkey-hide gelatin blocks at 80-90 ℃ and then melting, keeping the temperature at 60-70 ℃ after melting and maintaining the melting state, adding the donkey-hide gelatin composite polypeptide prepared by the method and high-quality donkey-hide gelatin at a mass ratio of 1:0.5, respectively adding black sesame, medlar, red dates and walnut at a mass ratio of 1:0.5:0.2:0.1, uniformly mixing and stirring, spreading, airing and cooling, and cutting into small blocks with the length of 6-8cm, the width of 3-4cm and the thickness of 1-1.5cm to obtain the donkey-hide gelatin polypeptide paste.
The invention has the following beneficial effects:
1. through a large number of experiments, the team unexpectedly finds that the specific compound donkey-hide gelatin formula is fermented by adopting specific trichoderma and saccharomycetes so as to remove unnecessary components or enhance the drug effect, and the beneficial substances in the traditional Chinese medicine are fully extracted.
2. The donkey-hide gelatin polypeptide can be effectively obtained by mixing and co-fermenting high-quality donkey-hide gelatin and Chinese medicinal fermentation broth.
3. The donkey-hide gelatin prepared by the invention has unexpected effect of promoting milk secretion.
4. The donkey-hide gelatin composite polypeptide prepared by the invention can be used for preparing donkey-hide gelatin polypeptide paste, oral liquid, decoction, tablets and the like, and is very convenient to use.
5. Polygonatum sibiricum has the effects of invigorating qi, nourishing yin, strengthening spleen, moistening lung and tonifying kidney. Angelica sinensis is widely used in traditional Chinese medicine, is considered to be capable of conditioning female reproductive system, promoting uterine contraction and recovery, and is beneficial to recovery of menstruation and health of uterus. Fructus Lycii contains antioxidant and multiple vitamins, and helps to enhance immune system and relieve puerperal fatigue. Dendrobium officinale flower can refresh and resolve depression, is beneficial to relieving mental stress, keeping consciousness, relieving mental depression and preventing postpartum depression. Radix Puerariae has effects of nourishing qi and blood, nourishing yin and clearing heat. Ginger is considered to have anti-inflammatory and analgesic effects, and can prevent inflammation of wound and promote wound healing.
Drawings
FIG. 1 is a graph showing the comparison of the hydrolysis rates of donkey-hide gelatin of comparative example 1 and comparative example 2, according to the present invention.
FIG. 2 is a graph showing the comparison of the hydrolysis rate of colla Corii Asini in example 2 and comparative examples 3-5 according to the present invention.
Detailed Description
Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention. It should be noted that the following embodiments and features in the embodiments may be combined with each other without conflict.
Example 1
S1, preprocessing donkey-hide gelatin, namely selecting high-quality donkey-hide gelatin as a raw material, ensuring the purity and quality of the donkey-hide gelatin, and crushing the donkey-hide gelatin into 400-500-mesh particles for later use;
S2, preparing Trichoderma CGMCC 3.3711 bacterial liquid, namely preparing Trichoderma CGMCC 3.3711 bacterial liquid with the concentration of 1.5X10 9 cfu/ml, preparing saccharomycete CMCC (B) 98007 bacterial liquid, namely preparing saccharomycete CMCC (B) 98007 bacterial liquid with the concentration of 3.5X10 9 cfu/ml for later use;
S3, pretreatment of rhizoma polygonati, chinese angelica, medlar, dendrobium candidum flower, kudzuvine root and ginger, wherein the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium candidum flower, the kudzuvine root and the ginger are crushed according to the same mass ratio, are uniformly mixed, are mixed with water according to the mass ratio of 1:1.5, are then added with trichoderma liquid and saccharomycete liquid prepared in the step S2, are filled into a cylinder and are fermented, wherein the traditional Chinese medicine mixture is trichoderma liquid and saccharomycete liquid according to the mass ratio of 1:0.5:0.5, the fermentation temperature is 32 ℃, and the fermentation time is 12 hours;
S4, filtering after fermentation is completed, taking supernatant, pouring the supernatant into the donkey-hide gelatin melted in the step S1, uniformly stirring at the temperature of 32 ℃, and then keeping the temperature of 32 ℃ for fermentation for 6-12 hours to obtain donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, wherein the fermentation liquor is added according to the mass ratio of 1:1;
S5, after fermentation is completed, 3%H 2O2 solution is added to inactivate trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007, then donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor is filtered to remove solid particles and impurities, then 2mM NaOH is added to promote precipitation of donkey-hide gelatin polypeptide, then centrifugation is carried out, precipitate is collected after centrifugation, and the collected precipitate is dried at 30 ℃ to obtain the donkey-hide gelatin composite polypeptide for postpartum recovery.
Comparative example 1 the same procedure as in example 1 was followed except that the Trichoderma CGMCC 3.3711 bacterial liquid was not added in step S3.
Comparative example 2 the procedure was the same as in example 1 except that the bacterial liquid of the yeast CMCC (B) 98007 was not added in the step S3.
FIG. 1 is a graph showing the comparison of the hydrolysis rates of donkey-hide gelatin of comparative example 1 and comparative example 2, according to the present invention. According to the invention, the traditional Chinese medicine fermentation liquid which is not subjected to fungus inactivation is directly mixed with the high-quality donkey-hide gelatin, the traditional Chinese medicine fermentation liquid and the high-quality donkey-hide gelatin are co-fermented by using the trichoderma CGMCC 3.3711 and the saccharomycete CMCC (B) 98007 in the traditional Chinese medicine fermentation liquid, and the donkey-hide gelatin is decomposed into donkey-hide gelatin polypeptide under the action of the trichoderma CGMCC 3.3711 and the saccharomycete CMCC (B) 98007 during fermentation. As can be seen from fig. 1, the hydrolysis degree of donkey-hide gelatin is significantly reduced without using the trichoderma CGMCC 3.3711 bacterial liquid or the saccharomycete CMCC (B) 98007 bacterial liquid, which indicates that the significant synergistic effect between the trichoderma CGMCC 3.3711 and the saccharomycete CMCC (B) 98007 can promote the hydrolysis of donkey-hide gelatin, thereby obtaining more donkey-hide gelatin peptide.
Example 2
S1, preprocessing donkey-hide gelatin, namely selecting high-quality donkey-hide gelatin as a raw material, ensuring the purity and quality of the donkey-hide gelatin, and crushing the donkey-hide gelatin into 400-500-mesh particles for later use;
S2, preparing Trichoderma CGMCC 3.3711 bacterial liquid, namely preparing Trichoderma CGMCC 3.3711 bacterial liquid with the concentration of 3 multiplied by 10 9 cfu/ml, preparing saccharomycete CMCC (B) 98007 bacterial liquid, namely preparing saccharomycete CMCC (B) 98007 bacterial liquid with the concentration of 4.6multiplied by 10 9 cfu/ml for later use;
S3, pretreatment of rhizoma polygonati, chinese angelica, medlar, dendrobium candidum flower, kudzuvine root and ginger, wherein the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium candidum flower, the kudzuvine root and the ginger are crushed according to the same mass ratio, are uniformly mixed, are mixed with water according to the mass ratio of 1:1.5, and are then added with trichoderma liquid and saccharomycete liquid prepared in the step S2 for jar fermentation, wherein the traditional Chinese medicine mixture comprises the trichoderma liquid and the saccharomycete liquid according to the mass ratio of 2:1:1, the fermentation temperature is 35 ℃, and the fermentation time is 24 hours;
S4, filtering after fermentation is completed, taking supernatant after filtration, pouring the supernatant into the donkey-hide gelatin melted in the step S1, uniformly stirring at the temperature of 35 ℃, and then keeping the temperature of 35 ℃ for fermentation for 12 hours to obtain donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, wherein the fermentation liquor is added according to the mass ratio of donkey-hide gelatin of 1:1;
S5, after fermentation is completed, adding 3%H 2O2 solution to inactivate trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007, filtering donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, removing solid particles and impurities, adding 2mM NaOH to promote precipitation of donkey-hide gelatin polypeptide, centrifuging, completing centrifugation, collecting precipitate, and drying the collected precipitate at 30-40 ℃ to obtain the donkey-hide gelatin composite polypeptide for postpartum recovery.
Comparative example 3 the procedure was the same as in example 2, except that the fermentation broth of the traditional Chinese medicine in step S4 was replaced with hot water for stirring.
Comparative example 4 the steps were the same as in example 2 except that the trichoderma CGMCC 3.3711 in step S2 was replaced with trichoderma CGMCC3.2942 (purchased from bio-technology limited of baposi, beijing).
Comparative example 5 the procedure was the same as in example 2 except that yeast CMCC (B) 98007 in step S2 was replaced with yeast ATCC9763 (available from Ruichu Biotechnology (Jiangsu) Co., ltd.).
FIG. 2 is a graph showing the comparison of the hydrolysis rate of colla Corii Asini in example 2 and comparative examples 3-5 according to the present invention. According to the second graph, in the embodiment 2, the decomposition rate can reach 67% by adopting a fermentation decomposition mode, and the hydrolysis rate is obviously reduced by adopting a hydrolysis mode of hot water stirring, so that the fungus decomposition preference is shown. From the results of comparative examples 4 and 3, it is understood that Trichoderma CGMCC 3.3711 and yeast CMCC (B) 98007 are specific to decomposing donkey-hide gelatin, and that the synergistic effect between the two bacteria is also specific.
Example 3
S1, preprocessing donkey-hide gelatin, namely selecting high-quality donkey-hide gelatin as a raw material, ensuring the purity and quality of the donkey-hide gelatin, and crushing the donkey-hide gelatin into 400-500-mesh particles for later use;
S2, preparing Trichoderma CGMCC 3.3711 bacterial liquid, namely preparing Trichoderma CGMCC 3.3711 bacterial liquid with the concentration of 2.2X10 9 cfu/ml, preparing saccharomycete CMCC (B) 98007 bacterial liquid, namely preparing saccharomycete ATCC 12845 bacterial liquid with the concentration of 3.9X10 9 cfu/ml for later use;
s3, pretreatment of rhizoma polygonati, chinese angelica, medlar, dendrobium officinale flower, kudzuvine root and ginger, wherein the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium officinale flower, the kudzuvine root and the ginger are crushed according to the same mass ratio and then are uniformly mixed with water according to the mass ratio of 1:1.5, and then the mixture is added with trichoderma liquid and saccharomycete liquid prepared in the step S2 for jar fermentation, wherein the traditional Chinese medicine mixture comprises the trichoderma liquid and the saccharomycete liquid according to the mass ratio of 1:0.5:0.5, the fermentation temperature is 33 ℃, and the fermentation time is 18 hours;
S4, filtering after fermentation is completed, taking supernatant, pouring the supernatant into the donkey-hide gelatin melted in the step S1, uniformly stirring at the temperature of 33 ℃, and then keeping the temperature of 32-35 ℃ for fermentation for 6-12 hours to obtain donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquid, wherein the mass ratio of donkey-hide gelatin to fermentation liquid is 1:1;
S5, after fermentation is completed, 3%H 2O2 solution is added to inactivate trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007, then donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor is filtered to remove solid particles and impurities, then 2mM NaOH is added to promote precipitation of donkey-hide gelatin polypeptide, then centrifugation is carried out, precipitate is collected after centrifugation, and the collected precipitate is dried at 35 ℃ to obtain the donkey-hide gelatin composite polypeptide for postpartum recovery.
Comparative example 6 the steps were the same as in example 3 except that the fermentation was performed without adding the Chinese medicine in step S3.
Comparative example 7 the respective steps were the same as in example 3 except that kudzuvine root was not added in step S4.
Comparative example 8 the procedure was the same as in example 3, except that no ginger was added in step S4.
Example 4
Animal experiment
Lactation conditioning experiment
Grouping, modeling and administration of animals
SPF-grade healthy KM breeds pregnant mice (supplied by the biological technology limited company of St Bei Fu (Beijing)), 70 animals are subjected to animal production license SCXK (Beijing) 2016-0002, 130 days old, and the weight is 92+/-11 g, and the mice are fed into SPF-grade animal feeding rooms of the company, are relatively constant in temperature (25 ℃) and humidity (75%), are fed with conventional mouse pellet feed, and are fed with water freely.
55 Mice with a difference of not more than 24h before and after the birth time were taken as experimental study subjects, and each litter was adjusted to 5 mice. The rats were randomly divided into a lack of milk model group, a normal group, a positive control estradiol group and an example group (example 3), and 11 groups of comparative examples (comparative example 1, comparative example 2, comparative example 4, comparative example 5, comparative example 6, comparative example 7 and comparative example 8) were used, each group being 5.
The day of delivery is 0 d, from 3 rd d after the delivery of the female mice, except for the normal group, the rest groups are filled with bromocriptine (1.6 mg/kg) daily to establish a model of postpartum hypogalactia of the mice, which is 10 d. At the time of molding, according to the human and animal dose change algorithm, example group (example 3), comparative example group (comparative example 1, comparative example 2, comparative example 4, comparative example 5, comparative example 6, comparative example 7 and comparative example 8) were respectively given example 3, comparative example 1, comparative example 2, comparative example 4, comparative example 5, comparative example 6, comparative example 7 and comparative example 8 prepared donkey-hide gelatin composite polypeptide 1.2g/100 g.d was fed, and estradiol group was given 17 a-estradiol (1 g/kg), normal group and lack milk model group were given an equal amount of physiological saline. After gastric lavage 10 d (postpartum 13 th d), the female mice were sacrificed and blood and mammary tissue were taken for index detection. And (3) measuring the weight increment of the litter of the mice by referring to the method in the prior art, and detecting the lactation yield and the PRL.
(3) Index detection
And (3) measuring the litter weight increment, namely weighing the last litter weight minus the first litter weight after the litter weight increment of the mice is 10 d th.
Lactation amount is measured by weighing the whole litter (W1) at a fixed time every day from the third day of administration, isolating the litter from the mother 4 h, weighing again (W2), and then weighing for the third time after feeding 1h in a cage with the mother (W3). The hourly lactation amount of the female mice was calculated by taking the weight difference (W3-W2) of the whole litter mice before and after 1 hour of lactation, and adding the average basal metabolic amount ((W1-W2)/4) of the whole litter mice for 1 hour. I.e. milk yield per hour = w3-w2+ (W1-W2)/4.
The weight gain results of the litter are shown in Table 1:
TABLE 1
The lactation yield (mL) is shown in table 2:
TABLE 2
From the results of increasing litter weight and lactation yield of the mice in the milk deficiency model group in tables 1 and 2, respectively, it was found that the milk deficiency model was constructed successfully. From example 3, the results of comparative examples 1 and 2 show that the weight gain of litter and lactation yield of the mice are significantly reduced when fermentation is performed without using trichoderma CGMCC 3.3711 or yeast CMCC (B) 98007, indicating that the synergy between trichoderma CGMCC 3.3711 and yeast CMCC (B) 98007 can promote milk secretion. From example 3, comparative example 4 and comparative example 5, it is known that trichoderma CGMCC 3.3711 and yeast CMCC (B) 98007 are specific for promoting milk fermentation. According to example 3, comparative example 6, comparative example 7 and comparative example 8, the addition of the fermented product of the traditional Chinese medicine has unexpected effect of promoting the milk secretion, and the simultaneous addition of the kudzuvine root and the ginger in the 6 medicines can produce synergistic effect, wherein the kudzuvine root can promote the milk secretion, the ginger can drive the blood circulation, and the milk secretion is quickened.
The above examples merely illustrate specific embodiments of the invention, which are described in more detail and are not to be construed as limiting the scope of the invention. It should be noted that, it should be understood that those skilled in the art should not depart from the spirit and principle of the present invention, any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention are all equivalents to be included in the scope of the present invention.
Claims (8)
1. A preparation method of a postpartum recovered donkey-hide gelatin composite polypeptide is characterized by comprising the following specific steps:
s1, preprocessing donkey-hide gelatin, namely crushing the donkey-hide gelatin for later use;
S2, preparing Trichoderma reesei (Trichoderma reesei) CGMCC 3.3711 bacterial liquid, namely preparing Trichoderma reesei CGMCC 3.3711 bacterial liquid with the concentration of 1.5X10 9~3×109 cfu/ml, preparing Saccharomyces cerevisiae (Saccharomyces cerevisiae) CMCC (B) 98007 bacterial liquid, namely preparing Saccharomyces cerevisiae CMCC (B) 98007 bacterial liquid with the concentration of 3.5X10 9~4.6×109 cfu/ml for later use;
S3, pretreatment of rhizoma polygonati, chinese angelica, medlar, dendrobium candidum flowers, kudzuvine roots and ginger, wherein the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium candidum flowers, the kudzuvine roots and the ginger are crushed and then are uniformly mixed with water according to the mass ratio of 1:1.5, then trichoderma liquid and saccharomycete liquid prepared in the step S2 are added into a jar for fermentation, the fermentation temperature is 32-35 ℃, and the fermentation time is 12-24 hours;
S4, filtering after fermentation is completed, taking supernatant, adding the donkey-hide gelatin crushed in the step S1, uniformly stirring at the temperature of 32-35 ℃, and then keeping the temperature of 32-35 ℃ for fermentation for 6-12 hours to obtain donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, wherein the mass ratio of donkey-hide gelatin to fermentation liquor is 1:1;
S5, after fermentation is completed, adding 3%H 2O2 solution to inactivate trichoderma CGMCC 3.3711 and saccharomycete CMCC (B) 98007, filtering donkey-hide gelatin polypeptide liquid containing traditional Chinese medicine fermentation liquor, removing solid particles and impurities, adding 2mM NaOH to promote precipitation of donkey-hide gelatin polypeptide, centrifuging, completing centrifugation, collecting precipitate, and drying the collected precipitate at 30-40 ℃ to obtain the donkey-hide gelatin composite polypeptide for postpartum recovery.
2. The method for preparing the postpartum recovered donkey-hide gelatin composite polypeptide according to claim 1, wherein the concentration of the Trichoderma reesei CGMCC 3.3711 bacterial liquid used in the step S2 is 2.2X10 9 cfu/ml.
3. The method for preparing a post-partum recovered donkey-hide gelatin composite polypeptide according to claim 1, wherein the concentration of the beer yeast CMCC (B) 98007 bacterial liquid used in the step S2 is 3.9X10 9 cfu/ml.
4. The method for preparing the postpartum recovery donkey-hide gelatin composite polypeptide of claim 3, wherein the step S3 is characterized in that the rhizoma polygonati, the Chinese angelica, the medlar, the dendrobium candidum flower, the kudzuvine root and the ginger are crushed according to the same mass ratio and then are uniformly mixed.
5. The method for preparing a post-partum recovered donkey-hide gelatin composite polypeptide according to claim 4, wherein in the step S3, the Chinese medicinal mixture of trichoderma liquid and saccharomycete liquid is uniformly mixed according to a mass ratio of 1:0.5:0.5.
6. The method for preparing a post-partum recovered donkey-hide gelatin composite polypeptide according to claim 5, wherein the supernatant obtained in the step S4 is not inactivated, and is directly mixed with the melted donkey-hide gelatin obtained in the step S1 for fermentation.
7. The donkey-hide gelatin composite polypeptide prepared by the preparation method according to any one of claims 1 to 6.
8. A preparation method of donkey-hide gelatin paste is characterized in that the donkey-hide gelatin composite polypeptide of claim 7 is used for preparing donkey-hide gelatin composite polypeptide paste;
the preparation method comprises the following steps:
Heating high-quality donkey-hide gelatin blocks at 80-90 ℃ and then melting, keeping the temperature at 60-70 ℃ and maintaining the melting state after melting, adding the donkey-hide gelatin composite polypeptide of claim 7 into the high-quality donkey-hide gelatin according to the mass ratio of 1:0.5, respectively adding black sesame, medlar, red dates and walnut according to the mass ratio of 1:0.5:0.2:0.2:0.1 into the donkey-hide gelatin composite polypeptide, uniformly mixing and stirring the donkey-hide gelatin composite polypeptide, spreading and cooling, and cutting the donkey-hide gelatin composite polypeptide into small blocks with the length of 6-8cm, the width of 3-4cm and the thickness of 1-1.5 cm.
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