CN117031048A - Full-automatic homogeneous phase chemiluminescence detector and testing method - Google Patents

Abstract

The invention provides a full-automatic homogeneous phase chemiluminescence detector and a testing method, wherein the full-automatic homogeneous phase chemiluminescence detector comprises a rack and an equipment rack for accommodating other modules; the device comprises a sample platform, a reaction cup module, a reagent storage unit, an incubation module, a sample adding unit, an optical detection unit, a reaction cup taking and placing unit, a waste bin, a mixing unit, a cleaning tank and a test tube clamping unit, which are arranged on a rack; according to the invention, through the mutual combination of the upper computer unit and the middle-lower computer unit, after the instrument is started to enter a working state, each reagent component and sample are automatically added into the reaction container, the reaction is fully incubated, and finally, the test result of the detection item is output, so that the manual operation of a worker is not required, the working efficiency is improved, the problems of uncertainty such as individual difference, manual error, operation specification and the like in manual operation can be effectively avoided, and the accuracy and consistency of chemiluminescent immunoassay are improved.

Description

Full-automatic homogeneous phase chemiluminescence detector and testing method

Technical Field

The invention relates to a full-automatic homogeneous phase chemiluminescence detector and a testing method, and belongs to the technical field of chemiluminescence immunoassay.

Background

The chemiluminescent instrument is an analytical instrument used in the field of basic medicine, adopts a chemiluminescent technology, is one of the most mainstream methods in the current immunoassay technology, and is based on the principle that a chemical reaction, usually an oxidation reaction, generates a substance with an electron energy level in an excited state, and the substance generates photons through transition release energy so as to generate a luminescent phenomenon; it is characterized by consuming luminescent agent and simultaneously relatively decreasing quantum efficiency.

Traditional chemiluminescence can be classified into direct chemiluminescence and indirect chemiluminescence according to chemical reaction types, and belongs to the field of heterogeneous immunoassay, and unbound substances need to be separated before detection.

The immunoassay methods currently used clinically are mostly of this type. The experimental modes are identical: that is, not only is it mostly necessary to solidify one of the immunologically active substances onto the carrier; it is also desirable to attach another immunologically active substance to a marker molecule that produces a specific signal that is derived from the marker.

From the 90 th of the 20 th century, a homogeneous phase light-emitting system is gradually developed, compared with the traditional light-emitting technology, unbound substances are not required to be separated, the cleaning and separating process and structure of an instrument are reduced, the whole test time is shortened, the output result is faster, the systematic error of the reaction is reduced, the whole energy generating, transmitting and amplifying process is quite stable, and the influence of pH value, ionic strength and temperature is not easy to be influenced.

However, the existing homogeneous chemiluminescent detector has large equipment space occupation, single function, or only single incubation function, or else only cleaning and separation functions, and no reaction cup module function.

Therefore, the invention provides the full-automatic homogeneous phase chemiluminescence detector with high degree of automation, small volume, simple operation and high testing accuracy and the testing method.

Disclosure of Invention

In view of the above, the invention provides a full-automatic homogeneous chemiluminescent detector with high degree of automation, small volume, simple operation and high testing accuracy and a testing method.

The invention is realized in the following way:

a full-automatic homogeneous chemiluminescent detector comprises a rack, an equipment rack for accommodating other modules;

the device comprises a sample platform, a reaction cup module, a reagent storage unit, an incubation module, a sample adding unit, an optical detection unit, a reaction cup taking and placing unit, a waste bin, a mixing unit, a cleaning tank and a test tube clamping unit, which are arranged on a rack;

a mixing unit is arranged in the middle of the sample platform and is used for automatically mixing the whole blood sample;

the sample platform is connected to one end of the bottom of the rack and carries a plurality of test tubes to be tested;

the other end of the frame opposite to the sample platform is provided with a reaction cup module for loading reaction cups;

a reagent storage unit for placing a reagent bottle is arranged between the sample platform and the reaction cup module, and a reagent for testing is stored;

the incubation module and the optical detection unit are sequentially and horizontally arranged on the right side of the reagent storage unit, the incubation module is used for incubating the reaction solution to be detected at a constant temperature, and the optical detection unit is used for detecting the test result of the reactant to be detected;

a sample adding unit is arranged on a square rack of the incubation module and is used for taking charge of sucking and discharging reagents and samples;

a reaction cup taking and placing unit and a waste bin are arranged right below the optical detection unit, and the reaction cup taking and placing unit is used for grabbing the reaction cup to a detection position for photometry and grabbing the waste reaction cup after the test; the waste bin is used for containing waste reaction cups.

A test tube clamping unit is arranged at the included angle of the sample platform, the reagent storage unit and the incubation module, and is used for clamping a test tube to be tested and matching with the mixing unit to open or close the cover;

a cleaning pool for cleaning the sample adding needle is arranged beside the test tube clamping unit and is used for cleaning the sample adding needle;

the control module is externally arranged on the rack and used for controlling and driving the lower computer units, connecting the lower computer units to communicate with the computer, and also responsible for time sequence adjustment of each action and state module and controlling and collecting data packets of each lower computer unit and packaging and transmitting the data packets.

The lower computer unit refers to a sample platform, a reaction cup module, a reagent storage unit, an incubation module, a sample adding unit, an optical detection unit, a reaction cup taking and placing unit, a waste bin, a mixing unit, a cleaning tank and a test tube taking unit.

The operation module is arranged on the industrial control board and the touch screen which are arranged on the rack externally, and is connected with the printer to print the test result; the front panel is provided with an RFID reading module for reading the reagent and calibration parameter information in the RFID card.

A testing method of a full-automatic homogeneous phase chemiluminescence detector comprises the following specific steps:

s1, a sample platform steps a sample rack to be tested to a sample sucking position, judges whether the sample rack is a whole blood sample or not, whether the sample rack is required to be mixed uniformly, extracts a sample test tube from a mixing unit, swings and mixes uniformly, then places the sample test tube to a uncapping position, and performs uncapping treatment by matching with the mixing unit; other types of samples hold the sample tube in the sample-sucking position;

s2, extracting a new one-piece reaction cup from the reaction cup module, pushing the new one-piece reaction cup to an incubation disc, and transferring the one-piece reaction cup to a sample adding position;

s3, a sampling needle of the sample adding unit is arranged at a sample sucking position, a sample is sucked in a test tube, the test tube is moved to a sample adding position of an incubation plate, the sample is discharged into the reaction cup, and then the sample adding needle is cleaned;

s4, the reagent tray rotates to a position of a reagent required by the test, the reagent 1 is sucked from the first reagent bottle and discharged into the reaction cup, and then the sample adding needle is cleaned; then the sample adding needle adds the reagent 2 of the second reagent bottle into the reaction cup in the same way, and the sample adding needle is cleaned;

s5, checking whether the project is a one-step method, if so, adding the reagent 3 in the third reagent bottle into the reaction cup, and then starting to rotate and mix the reaction liquid uniformly; if the two-step method item is adopted, directly executing rotation mixing operation;

s6, checking whether the two-step method item in the incubation plate reaches the time of adding the reagent 3, and adding the reagent 3 in the third reagent bottle into the reaction cup if the two-step method item reaches the time, and continuing mixing and incubation;

s7, checking whether the reaction cup in the incubation tray reaches the condition of testing completion, if so, turning the cup to the position of the cup outlet of the incubation tray, and dragging the cup into the optical module by the mechanical arm of the reaction cup taking and placing unit, carrying out a light excitation test, acquiring a test result, and after the test is completed, throwing the cup into a waste bin.

The beneficial effects of the invention are as follows:

according to the invention, through the mutual combination of the upper computer unit and the middle-lower computer unit, after the instrument is started to enter a working state, each reagent component and sample are automatically added into the reaction container, the reaction is fully incubated, and finally, the test result of the detection item is output, so that the manual operation of a worker is not required, the working efficiency is improved, the problems of uncertainty such as individual difference, manual error, operation specification and the like in manual operation can be effectively avoided, and the accuracy and consistency of chemiluminescent immunoassay are improved.

The invention adopts a sample adding needle to add reagent and sample, a reagent disk, an incubation disk, a sample platform to automatically enter and exit the sample, a laser and a PMT module, and combines a control module and a computer on the basis of ensuring the small and rapid instrument to the greatest extent, thereby increasing the degree of automation and the rapid output of results.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the overall structure of a full-automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a sample platform in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a reaction cup module in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a reagent storage unit in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of an incubation module in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a sample loading unit of a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of an optical detection unit in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of a cuvette pick-and-place unit in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of a waste bin in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 10 is a schematic diagram of a mixing unit in a fully automatic homogeneous light emitting tester according to an embodiment of the present invention;

FIG. 11 is a schematic diagram of a cleaning tank in a fully automatic homogeneous luminescence tester according to an embodiment of the present invention;

FIG. 12 is a schematic diagram of a test tube clamping unit in a fully automatic homogeneous light emission tester according to an embodiment of the present invention;

in the figure: the sample rack comprises a rack 10, a sample platform 100, a sample rack bottom plate 101, a first pusher 102, a sample rack pulling piece 103, a photoelectric sensor 104, a second pusher 105 and a micro switch;

the reaction cup module 200, a cup inlet bin 201, a cup outlet bin 202, a reaction cup lifting mechanism 210, a reaction cup ejector rod mechanism 220, a reaction cup shifting piece mechanism 230 and a reaction cup sample injection mechanism 240;

a reagent storage unit 300, a reagent disk drive assembly 310, a reagent tray 320, a reagent pot 330, and an air duct 340;

incubation module 400, first motor 401, transmission 410, incubation tray 420, incubation pot 430;

the sample adding unit 500, the guide rail 501, the sampling needle 502, the second motor 503, the Y-axis frame 510 and the mounting block 520;

an optical detection unit 600, a laser 601, a light blocking sheet 603, and a PMT module 604;

a reaction cup taking and placing unit 700, a base 701, a third motor 702 and a manipulator 703;

a waste bin 800, a waste bin 801, a bin full detection sensor 802, and a waste bin cover plate 803;

mixing unit 900, fourth motor 901, fifth motor 902, Z-axis assembly 910, Y-axis assembly 920, second manipulator 930;

a cleaning tank 1000;

a test tube gripping unit 1100, a sixth motor 1101, a cup holder 1102, and a third robot 1103.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.

In the description of the present invention, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

Referring to fig. 1-12, the present embodiment provides a full-automatic homogeneous chemiluminescent detector according to one embodiment, comprising a rack 10 for receiving equipment racks of other modules;

the sample platform 100, the reaction cup module 200, the reagent storage unit 300, the incubation module 400, the sample adding unit 500, the optical detection unit 600, the reaction cup picking and placing unit 700, the waste bin 800, the mixing unit 900, the cleaning tank 1000 and the test tube clamping unit 1100 are arranged on the rack 10;

a mixing unit 900 is installed in the middle of the sample platform 100, and is used for automatically mixing the whole blood sample;

the sample platform 100 is connected to one end of the bottom of the rack 10 and carries a plurality of test tubes to be tested;

the other end of the frame 10 opposite to the sample platform 100 is provided with a cuvette module 200 for loading a cuvette;

a reagent storage unit 300 for storing a reagent for testing is installed between the sample platform 100 and the cuvette module 200;

the incubation module 400 and the optical detection unit 600 are sequentially and horizontally arranged on the right side of the reagent storage unit 300, the incubation module 400 is used for incubating the reaction solution to be detected at a constant temperature, and the optical detection unit 600 is used for detecting the test result of the reaction solution to be detected; the reaction solution to be measured herein refers to a mixture of a reagent and a sample.

A sample adding unit 500 is installed on the square rack 10 of the incubation module 400 and is used for absorbing and discharging reagents and samples;

a reaction cup taking and placing unit 700 and a waste bin 800 are arranged right below the optical detection unit 600, and the reaction cup taking and placing unit 700 is used for grabbing reaction cups to a detection position for photometry and grabbing waste reaction cups after the test; the waste bin 800 is used to hold waste reaction cups.

A test tube clamping unit 1100 is installed at the included angle between the sample platform 100, the reagent storage unit 300 and the incubation module 400, and is used for clamping a test tube to be tested and matching with the mixing unit 900 to open or close the cover;

a cleaning tank 1000 for cleaning the test tube is installed beside the test tube clamping unit 1100 for cleaning the sample adding needle;

the control module is externally arranged on the frame 10 and used for controlling and driving the lower computer units, connecting the lower computer units to communicate with the computer, and also responsible for time sequence adjustment of each action and state module and controlling the collection and packaging of data packets of each lower computer unit, and the control module is also an upper computer unit.

The lower unit as described herein refers to the sample platform 100, the cuvette module 200, the reagent storage unit 300, the incubation module 400, the sample loading unit 500, the optical detection unit 600, the cuvette pick-and-place unit 700, the waste bin 800, the mixing unit 900, the cleaning cell 1000, and the test tube pick-up unit 1100.

The system also comprises an operation module, an industrial control board and a touch screen which are arranged on the frame 10 externally, and the operation module is connected with a computer and a printer and is used for printing test results and reading RFID information.

Referring to fig. 1 and 2, the sample platform 100 includes a sample rack base plate 101, a first pusher 102, a sample rack pulling piece 103, a photoelectric sensor 104, a second pusher 105, and a micro switch; the left and right sides of the sample rack bottom plate 101 are respectively provided with a sample outlet area and a sample inlet area for placing the sample rack, a stepping area is arranged in the area between the sample outlet area and the sample inlet area, and a mixing unit 900 is arranged at the front end of the stepping area.

During testing, a first pusher 102 arranged on a sample rack bottom plate 101 at the front end of the sample injection area pushes the sample racks, and a micro switch arranged at the rear end of the sample injection area can detect whether the sample racks move in place or not, and each sample rack is provided with a plurality of test tubes; after the sample rack is in place, a sample rack poking plate 103 arranged at the left side of the micro switch drives the sample rack to sequentially advance to a test tube position, the test tube passing through successively moves from left to right, the test tube is vertically arranged at a plurality of photoelectric sensors 104 beside the sample rack poking plate 103 through a supporting rod to step to a sample sucking position, and the sample sucking position is also a mixing tube taking position.

In one embodiment, 7 test tube positions are stepped to reach the sample sucking position.

The sample suction position is a position where the third robot 1103 of the test tube gripping unit 1100 can grip.

After the test is finished, the sample rack is pushed to the sample outlet area by the sample rack pulling piece 103, and is pushed out by the second pusher 105 arranged at the rear end of the sample outlet area, so that the sample rack is convenient to take.

In one embodiment, the first pusher 102 and the second pusher 105 may be electric telescopic rods or electric guide rods, and are not limited to the above two pushers, and may be used to push the sample rack in the case of power-on.

Further, the first pusher 102 and the second pusher 105 are connected to the control module, and push the sample rack.

Referring to fig. 1 and 3, the cuvette module 200 includes a cuvette inlet bin 201, a cuvette outlet bin 202, a cuvette lifting mechanism 210, a cuvette ejector mechanism 220, a cuvette pulling plate mechanism 230, and a cuvette sample injection mechanism 240; the cup feeding bin 201 and the cup discharging bin 202 are arranged on the same side, the cup feeding bin 201 is used for accommodating reaction cups filled with reaction cups, the cup discharging bin 202 is used for accommodating empty reaction cups, the reaction cups are placed in the cup feeding bin 201, the reaction cups are lifted by the reaction cup lifting mechanism 210 vertically arranged on the cup feeding bin 201, then are pulled to the linear position of the cup discharging bin 202 by the reaction cup pulling piece mechanism 230, and finally the reaction cup ejector rod mechanism 220 is ejected from the cup discharging bin 202.

In one embodiment, the reaction cup lifting mechanism 210 is a worm and gear lifter, the reaction cup ejector rod mechanism 220 is composed of a screw motor and a pushing block installed at the output end of the screw motor, and the screw motor operates to drive the pushing block to push the reaction cup out.

Further, a cuvette injection mechanism 240 is installed at the left end of the cuvette ejector mechanism 220 near the cuvette outlet bin 202, and is used for pushing the cuvette to the feeding position of the cuvette, and the cuvette ejector mechanism 220 pushes the cuvette out.

Referring to fig. 1 and 4, the reagent storage unit 300 includes a reagent disk driving assembly 310, a reagent tray 320, a reagent pot 330, and an air duct 340; a reagent tray 320 is assembled in the reagent pot 330, and a plurality of reagent bottle devices are placed on the reagent tray 320, wherein the reagent bottle devices are that a first reagent bottle, a second reagent bottle and a third reagent bottle are connected into a whole through a reagent bottle rack; the reagent tray 320 is rotationally driven by a reagent tray transmission assembly 310 arranged right below the reagent tray 320, a refrigerating component is arranged at the bottom of the reagent pot 330 and is connected with a plurality of air channels 340, a fan is arranged at the tail end of each air channel 340, and the fan sucks and replaces hot air around the refrigerating component to realize heat dissipation and further realize low-temperature preservation of reagents.

In one embodiment, the reagent tray transmission assembly 310 is composed of a second motor and a rotating rod, the rotating rod is respectively connected with the second motor and the reagent tray 320, and the second motor is powered to operate so as to drive the reagent tray 320 to rotate.

In one embodiment, the refrigeration component includes, but is not limited to, a micro-refrigerator, which is a refrigeration device that generates a small amount of cold.

Referring to fig. 1 and 5, the incubation module 400 includes a first motor 401, a transmission member 410, an incubation tray 420, and an incubation pot 430, the incubation tray 420 is installed inside the incubation pot 430, the first motor 401 is installed at the bottom of the incubation tray 420 through the transmission member 410, and the incubation tray 420 is driven to rotate by the transmission member 410 after the first motor 401 is electrified, so as to achieve uniform mixing of the reaction solution.

The driving member 410 includes, but is not limited to, a coupling, a driving shaft, as long as it is capable of driving power and rotating the incubation plate 420.

Further, a heating element is installed below the incubation pan 430, so that the incubation pan 420 can be heated, and the incubation reaction solution is uniformly mixed at a certain temperature.

In one embodiment, the heating element includes, but is not limited to, a thermistor, the resistance of which changes when the temperature increases, and once the resistance changes, the resistance temperature caused by the thermal effect of the current changes, so that the temperature of the element is regulated by circulation, and the incubation plate 420 is kept in a constant temperature state all the time.

Further, a reaction cup may be loaded on the incubation plate 420 for incubation.

Referring to fig. 1 and 6, the sample loading unit 500 includes a guide rail 501, a sampling needle 502, a second motor 503, a Y-axis frame 510, and a mounting block 520, wherein the guide rail 501 is assembled at the top end of the Y-axis frame 510, the mounting block 520 slides horizontally and laterally on the guide rail 501, the sampling needle 502 is installed on the mounting block 520, the second motor 503 is installed at the top of the sampling needle 502, and the second motor 503 operates to drive the sampling needle 502 to move up and down.

Further, the second motor 503 is horizontally disposed on the mounting block 520 and mechanically connected to the sampling needle 502 in the Z-axis direction through a direction-changing transmission unit, where the direction-changing unit includes, but is not limited to, a three-star gear direction-changing mechanism and a bevel gear direction-changing mechanism, so long as the direction and transmission can be changed.

Referring to fig. 1 and 7, the optical detection unit 600 includes a laser 601, a light blocking sheet 603, and a PMT module 604, the light blocking sheet 603 is installed above the laser 601, and the PMT module 604 is disposed on the side surfaces of the light blocking sheet 603 and the laser 601;

a laser 601 for optical excitation to generate an optical signal;

a light blocking sheet 603 for adjusting the brightness of the laser light generated by the laser 601;

PMT module 604 is configured to detect the optical signal and output a photon count value via the counting module.

Referring to fig. 1 and 8, the reaction cup picking and placing unit 700 includes a base 701, a third motor 702, and a manipulator 703; a third motor 702 is arranged at one horizontal end of the end face of the base 701, a manipulator 703 is mounted at the right end of the base 701, and the manipulator 703 is mechanically connected with the third motor 702.

A robot 703 for gripping or discarding the cuvette.

Referring to fig. 1 and 9, the waste bin 800 includes a waste bin 801, a bin full detection sensor 802, and a waste bin cover 803; the top movable mounting of waste bin 801 has waste bin apron 803, and it has the import that supplies the reaction cup to throw in to open on the waste bin apron 803, waste bin 801 is inside still to be equipped with full detection sensor 802.

The bin full detection sensor 802 detects that the waste bin 801 is approaching full, and displays a bin full alarm.

Referring to fig. 1 and 10, the mixing unit 900 includes a fourth motor 901, a fifth motor 902, a Z-axis assembly 910, a Y-axis assembly 920, and a second manipulator 930, where the Z-axis assembly 910 is mounted on an inner end surface of the frame 10 through a support, the fourth motor 901 is mounted in the middle of the support and mechanically connected to the Z-axis assembly 910, the Y-axis assembly 920 and the Z-axis assembly 910 are vertically connected to each other and mechanically connected to the fifth motor 902, and the second manipulator 930 is mounted on the Y-axis assembly 920.

A fourth motor 901 for driving the Z-axis assembly 910 to vertically move up and down on the Z-axis;

a fifth motor 902, driving the Y-axis assembly 920 to move back and forth under the driving of the motor 902;

a second manipulator 930 for gripping or depositing the test tube to be tested;

a Z-axis assembly 910, a stationary Y-axis assembly 920;

the Y-axis assembly 920 holds a second robot 930.

Referring to fig. 1 and 11, the cleaning tank 1000 is used for cleaning the sampling needle 502.

Referring to fig. 1 and 12, the test tube clamping unit 1100 includes a sixth motor 1101, a cup holder 1102, and a third manipulator 1103, the test tube clamping unit 1100 is provided with the cup holder 1102, the cup holder 1102 is placed with a test tube to be tested, the third manipulator 1103 is mounted at two horizontal ends of the holder 1102, one of the third manipulator 1103 is mechanically connected with the sixth motor 1101 horizontally mounted beside the test tube clamping unit 1100, the sixth motor 1101 is a screw motor, and the third manipulator 1103 is opened and closed in the rotation process.

The test tube clamping unit 1100 is used for clamping or unclamping a test tube to be tested.

In summary, the first motor 401, the second motor 503, the third motor 702, the fourth motor 901, the fifth motor 902, and the sixth motor 1101 are all connected to the control module, so as to facilitate control by the control module.

Based on the above-mentioned test method of a full-automatic homogeneous phase chemiluminescence detector, the specific steps are as follows:

s1, a sample platform 100 steps a sample rack to be tested to a sample sucking position, judges whether the sample rack is a whole blood sample or not, whether the sample rack is required to be mixed uniformly, extracts a sample test tube from a mixing unit, swings and mixes the whole blood sample uniformly, then places the sample test tube at a cover opening position, and cooperates with the mixing unit 900 to perform cover opening treatment; other types of samples hold the sample tube in the sample-sucking position;

s2, extracting a new one-piece reaction cup from the reaction cup module 200, pushing the new one-piece reaction cup to the incubation plate 420, and transferring the one-piece reaction cup to a sample adding position;

s3, the sampling needle 502 of the sample adding unit 500 is placed at a sample sucking position, a sample is sucked in a test tube, the test tube is moved to a sample adding position of the incubation plate 420, the sample is discharged into the reaction cup, and then the sample adding needle 502 is cleaned;

s4, the reagent tray 320 rotates to a position of a reagent required by the test, the reagent 1 is sucked from the first reagent bottle and discharged into the reaction cup, and then the sample adding needle 502 is cleaned; then the sample adding needle adds the reagent 2 of the second reagent bottle into the reaction cup in the same way, and the sampling needle 502 is cleaned;

s5, checking whether the project is a one-step method, if so, adding the reagent 3 in the third reagent bottle into the reaction cup, and then starting to rotate and mix the reaction liquid uniformly; if the two-step method item is adopted, directly executing rotation mixing operation;

s6, checking whether the two-step method item in the incubation plate 420 reaches the time of adding the reagent 3, and adding the reagent 3 in the third reagent bottle into the reaction cup if the two-step method item reaches the time, and continuing mixing and incubation;

s7, checking whether the reaction cup in the incubation plate 420 reaches the condition of testing completion, if so, turning the cup to the cup outlet position of the incubation plate 4220, and pulling the cup into an optical module by a manipulator 703 of the reaction cup picking and placing unit 700 for light excitation testing and obtaining a test result, and after the test is completed, throwing the cup into a waste bin 800.

In step S7, if the waste bin 800 is close to the full bin, a bin full alarm is displayed, so that the waste bin 800 can be conveniently and timely emptied.

In a word, through the structure and the testing method, after the instrument is started to enter a working state, each reagent component and sample are automatically added into the reaction container, the reaction is fully incubated, and finally, the test result of the detection item is output, so that the manual operation of a worker is not needed, the working efficiency is improved, the problems of uncertainty such as individual difference, manual error, operation specification and the like existing in manual operation can be effectively avoided, and the accuracy and consistency of chemiluminescent immunoassay are improved.

And moreover, a sample adding needle is adopted to add reagents and samples, 1 reagent disc and an incubation disc are adopted, the sample platform 100 is formed by automatically feeding and discharging the samples, a laser and a PMT module, so that the automation degree and the rapid output of the result are improved on the basis of ensuring the small and rapid instrument to the greatest extent and combining a control module and a computer.

The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A fully automatic homogeneous chemiluminescent detector comprising a rack (10) for housing equipment racks of other modules; the device comprises a sample platform (100), a reaction cup module (200), a reagent storage unit (300), an incubation module (400), a sample adding unit (500), an optical detection unit (600), a reaction cup picking and placing unit (700), a waste bin (800), a mixing unit (900), a cleaning pool (1000) and a test tube picking unit (1100) which are arranged on a rack (10);

a mixing unit (900) is arranged in the middle of a sample platform (100), the sample platform (100) is connected to one end of the bottom of a rack (10), a reaction cup module (200) is arranged at the other end of the rack (10) opposite to the sample platform (100), a reagent storage unit (300) for placing a reagent bottle is arranged between the sample platform (100) and the reaction cup module (200), an incubation module (400) and an optical detection unit (600) are sequentially and horizontally arranged on the right side of the reagent storage unit (300), a sample adding unit (500) is arranged on the rack (10) of the incubation module (400), a reaction cup picking and placing unit (700) and a waste bin (800) are arranged right below the optical detection unit (600), a test tube picking unit (1100) is arranged at the included angle between the sample platform (100), the reagent storage unit (300) and the incubation module (400), and a cleaning pool (1000) for cleaning a sample needle is arranged beside the test tube picking unit (1100);

the control module is used for controlling and driving the lower computer units and connecting the lower computer units to communicate with the upper computer.

2. The fully automatic homogeneous chemiluminescent detector of claim 1 wherein the sample platform (100) comprises a sample rack base plate (101), a first pusher (102), a sample rack paddle (103), a photoelectric sensor (104), a second pusher (105), a microswitch; sample frame bottom plate (101) level left and right sides is respectively out appearance district and advances the district for place the sample frame go out the district between appearance district and the appearance district is the step zone, step zone front end is equipped with mixing unit (900), first impeller (102) of sample frame bottom plate (101) installation at the appearance district front end, installs the micro-gap switch in the appearance district rear end, installs sample frame plectrum (103) in the micro-gap switch left side, vertically installs a plurality of photoelectric sensor (104) aside sample frame plectrum (103) through branch.

3. The full-automatic homogeneous chemiluminescent detector of claim 2 wherein the cuvette module (200) is configured to house a cuvette, the reagent storage unit (300) comprises a reagent tray rotating assembly (310), a reagent tray (320), a reagent pot (330), and an air channel (340); a reagent tray (320) is assembled inside the reagent pot (330), and the reagent tray (320) is used for placing a plurality of connected reagent bottle devices; a reagent tray rotating assembly (310) is arranged right below the reagent tray (320), a refrigerating component is arranged at the bottom of the reagent pot (330), and a plurality of air channels (340) are connected with the refrigerating component.

4. A fully automatic homogeneous chemiluminescent detector according to claim 3 wherein the incubation module (400) comprises a first motor (401), a transmission member (410), an incubation plate (420), and an incubation pot (430), wherein the incubation plate (420) is installed in the incubation pot (430), and the first motor (401) is installed at the bottom of the incubation plate (420) through the transmission member (410).

5. The full-automatic homogeneous chemiluminescent detector of claim 4 wherein the sample loading unit (500) comprises a guide rail (501), a sampling needle (502), a second motor (503), a Y-axis frame (510) and a mounting block (520), wherein the guide rail (501) is assembled at the top end of the Y-axis frame (510), the mounting block (520) sliding horizontally and leftwards is mounted on the guide rail (501), the sampling needle (502) is mounted on the mounting block (520), and the second motor (503) is mounted at the top of the sampling needle (502).

6. The full-automatic homogeneous chemiluminescent detector of claim 5 wherein the optical detection unit (600) comprises a laser (601), a light blocking sheet (603) and a PMT module (604), wherein the light blocking sheet (603) is mounted above the laser (601), and PMT modules (604) are disposed on the sides of the light blocking sheet (603) and the laser (601).

7. A fully automated homogeneous chemiluminescent detector according to claim 6 wherein the laser (601) is configured to photoexcitation the reactant to produce an optical signal;

a light blocking sheet (603) for adjusting the brightness of the laser generated by the laser (601);

and the PMT module (604) is used for detecting the optical signal and outputting a photon counting value through the counting module.

8. The full-automatic homogeneous chemiluminescent detector of claim 7 wherein the cuvette pick-and-place unit (700) comprises a base (701), a third motor (702), and a manipulator (703); a third motor (702) is arranged at one horizontal end of the end face of the base (701), a manipulator (703) is arranged at the right end of the base (701), and the manipulator (703) is mechanically connected with the third motor (702);

and a manipulator (703) for gripping or discarding the cuvette.

9. The full-automatic homogeneous chemiluminescent detector of claim 9 wherein the test tube clamping unit (1100) comprises a sixth motor (1101), a cup holder (1102) and a third manipulator (1103), wherein the cup holder (1102) is arranged on the test tube clamping unit (1100), the cup holder (1102) is used for holding a test tube to be tested, the third manipulator (1103) is arranged at two horizontal ends of the holder (1102), and one of the third manipulators (1103) is mechanically connected with the sixth motor (1101) horizontally arranged beside the test tube clamping unit (1100).

10. A testing method based on the full-automatic homogeneous chemiluminescent detector of any one of claims 1 to 9, which is characterized by comprising the following specific steps:

s1, a sample platform (100) steps a sample rack to be tested to a sample suction position, judges whether the sample rack is a whole blood sample or not, whether the sample rack is required to be mixed uniformly, a sample test tube is extracted by a mixing unit to swing and mix uniformly, and then the sample rack is placed to a cover opening position and matched with a mixing unit (900) to carry out cover opening treatment; other types of samples hold the sample tube in the sample-sucking position;

s2, extracting a new one-piece reaction cup from the reaction cup module (200), pushing the new one-piece reaction cup to the incubation disc (420), and transferring the cup-piece reaction cup to a sample adding position;

s3, a sampling needle 502 of a sample adding unit (500) is arranged at a sample sucking position, a sample is sucked in a test tube, the test tube is moved to a sample adding position of an incubation plate (420), the sample is discharged into the reaction cup, and then the sample adding needle (502) is cleaned;

s4, the reagent tray (320) rotates to a position of a reagent required by the test, the reagent 1 is sucked from the first reagent bottle and discharged into the reaction cup, and then the sample adding needle (502) is cleaned; then the sample adding needle adds the reagent 2 of the second reagent bottle into the reaction cup in the same way, and the sample adding needle (502) is cleaned;

s5, checking whether the project is a one-step method, if so, adding the reagent 3 in the third reagent bottle into the reaction cup, and then starting to rotate and mix the reaction liquid uniformly; if the two-step method item is adopted, directly executing rotation mixing operation;

s6, checking whether the two-step method item in the incubation plate (420) reaches the time of adding the reagent 3, and adding the reagent 3 in the third reagent bottle into the reaction cup if the two-step method item reaches the time, and continuing uniformly mixing and incubation;

s7, checking whether the reaction cup in the incubation plate (420) reaches the condition of test completion, if so, turning the cup to the position of the cup outlet of the incubation plate (4220), and dragging the cup into an optical module by a manipulator (703) of a reaction cup taking and placing unit (700), carrying out a light excitation test, acquiring a test result, and throwing the cup out to enter a waste bin (800).