CN114441249A - Sperm liquefaction method, quality detection method and quality analyzer, storage medium - Google Patents

Abstract

A sperm liquefying method, a sperm quality detecting method, a sperm quality analyzer and a storage medium; the quality detection method comprises the following steps: acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list both comprise one or more sample information; triggering a liquefaction processing thread for executing the sample according to the liquefaction list to obtain first information, and adding the sample information after the liquefaction processing into the detection list; and starting to execute the detection processing thread of the sample according to the detection list to obtain second information, wherein the second information comprises the detection information of the sample. Through being compatible as an organic whole with liquefaction thread and detection thread, the user can freely select liquefaction thread or detection thread according to the condition of demand or semen sample to realize that multiple operation items such as liquefaction processing, liquefaction degree detection, the detection of sample are handled, both can improve detection efficiency, can satisfy the actual demand in the testing process again.

Description

Sperm liquefaction method, quality detection method and quality analyzer, storage medium

technical field

The invention relates to the technical field of medical detection, in particular to a sperm liquefaction method, a quality detection method, a quality analyzer and a storage medium.

Background technique

Sperm quality analysis is an important means of evaluating male fertility, and it is also the experimental basis for the diagnosis of andrological diseases and the observation of curative effects. Therefore, sperm quality analysis has very important guiding significance for medical clinical and scientific research projects. Among them, the sperm image is identified and tracked through the computer image processing function to accurately calculate and record the number, color, static characteristics and movement trajectory of the sperm, so as to obtain the dynamic and static parameters of the sperm, which is to complete the sperm quality detection and analysis. core link.

At present, the multifunctional sperm quality analyzers on the market generally only include the execution links such as sample injection, sampling, detection, and sample discarding. It has the detection function of a single item such as sperm color, sperm PH value, sperm count, etc.; it can not meet the actual detection needs of simultaneous multi-item detection, and it is easy to increase the cost of instrument purchase and use, and reduce the detection efficiency.

SUMMARY OF THE INVENTION

The main technical problem solved by the present invention is to provide a sperm liquefaction method, a sperm quality detection method, a multifunctional sperm quality analyzer, and a storage medium, so as to realize the purpose of multi-item detection of sperm and improve detection efficiency.

According to a first aspect, an embodiment provides a sperm liquefaction method, comprising:

the step of obtaining a liquefaction list, where the liquefaction list includes one or more sample information;

Triggering the liquefaction step, selecting one piece of the sample information from the liquefaction list, and triggering the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information;

The timing step is to time the liquefaction process of the sample to obtain the first time;

The liquefaction detection step is to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection;

The classification and disposal step is performed according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, the liquefaction result is output. If the liquefaction degree detection result is unqualified, it is judged whether the actual liquefaction time of the current sample reaches the third time. When the time reaches the third time, stop the liquefaction. If the actual liquefaction time of the current sample does not reach the third time, continue to perform the timing step and the liquefaction detection step. The second time is less than the third time, and the third time is a predetermined time. Set the maximum time allowed to liquefy.

According to a second aspect, an embodiment provides a sperm quality detection method, comprising:

obtaining a liquefaction list and a detection list, wherein the liquefaction list and the detection list each include one or more sample information;

Trigger the liquefaction processing thread for executing the sample according to the liquefaction list, obtain the first information, and add the sample information after the liquefaction process to the detection list;

Execute the detection processing thread of the sample according to the detection list, and obtain second information, where the second information includes the detection information of the sample;

Wherein, the liquefaction processing thread that triggers the execution of samples according to the liquefaction list includes:

Triggering the liquefaction step, selecting one piece of the sample information from the liquefaction list, and triggering the idle state in the liquefaction processing thread to enter the liquefaction execution state, so as to start the liquefaction process for the sample corresponding to the selected sample information;

The timing step is to time the liquefaction process of the sample to obtain the first time;

The liquefaction detection step is to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection;

The classification and disposal step is performed according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, the liquefaction result is output. If the liquefaction degree detection result is unqualified, it is judged whether the actual liquefaction time of the current sample reaches the third time. If the actual liquefaction time of the current sample does not reach the third time, the timing step and the liquefaction detection step are continued, and the second time is less than the third time. Three times, the third time is the preset maximum time allowed for liquefaction;

The first information is obtained according to the liquefaction result and/or prompt information.

In one embodiment, the classification and treatment step includes:

Compare the liquefaction degree detection result with a preset threshold;

If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result;

If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time of the current sample reaches the third time;

If the actual liquefaction time of the current sample does not reach the third time, the second time is extended to obtain a new second time, and the first time and the new second time are compared in the continuous execution of the liquefaction detection step.

In one embodiment, the classification and treatment step includes:

Compare the detection result of liquefaction degree with the preset threshold;

If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result;

If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time of the current sample reaches the third time;

If the liquefaction time of the current sample does not reach the third time, the first time is reset to zero, and the time is started from zero in the continuous execution of the timing step.

In one embodiment, after stopping the liquefaction, the method further includes: outputting prompt information indicating that the current sample is abnormally liquefied.

In one embodiment, the liquefaction degree detection result is the viscosity value of the semen sample.

In one embodiment, the performing liquefaction degree detection includes:

A sampling needle is driven to be inserted into the sample container corresponding to the sample information to draw the sample, and the viscosity value of the sample is detected by sensing the internal pressure of the sampling needle.

In one embodiment, the triggering of the detection processing thread for executing the sample according to the detection list, and obtaining the second information includes:

Trigger the reaction processing thread for executing the sample according to the detection list to obtain third information; and/or

Trigger the microscopic inspection processing thread of the execution sample according to the detection list to obtain fourth information;

The second information is obtained by using the third information and/or the fourth information.

According to a third aspect, an embodiment provides a multifunctional sperm quality analyzer, including a liquefaction processing mechanism, a sampling mechanism, a detection processing mechanism, and a controller;

The liquefaction processing mechanism is provided with an incubation heating component, which is used for heating and liquefying the samples in the sample container corresponding to each sample information in the liquefaction list through the incubation heating component;

The sampling mechanism is provided with a sampling needle, which is used to aspirate the sample in the sample container through the sampling, and perform liquefaction detection, and to aspirate the sample corresponding to each sample information in the detection list. , and add the drawn sample to the reaction vessel and/or to the test card;

The detection and processing mechanism is provided with a reaction container and a first imaging component, which is used to capture images of the samples before and after the injection of the reaction reagent in the reaction container through the first imaging component; and/or the detection and processing mechanism is provided with having a microscope and a second imaging component, used for focusing the sample on the detection card through the microscope, and capturing the sample on the detection card through the second imaging component;

The controller is signal-connected with the liquefaction processing mechanism, the sampling mechanism and the detection processing mechanism, and is used for monitoring the liquefaction processing mechanism, the sampling mechanism and the detection mechanism by the method described in the second aspect Perform timing control.

A computer-readable storage medium comprising a program executable by a processor to implement the method of the second aspect.

The beneficial effects of this application are:

A sperm quality detection method according to the above embodiment includes: acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list each include one or more sample information; triggering a liquefaction processing thread for executing samples according to the liquefaction list, and obtaining the first information, and adding the sample information after the liquefaction process to the detection list; starting and executing the detection processing thread of the sample according to the detection list to obtain second information, where the second information includes the detection information of the sample. By integrating the liquefaction thread and the detection thread into one, the user can freely select the liquefaction thread or the detection thread according to the needs or the condition of the semen sample, so as to realize the liquefaction processing of the sample, the detection of the degree of liquefaction, the detection processing of the sample and other operation items. The detection efficiency can be improved, and the actual demand in the detection process can be met.

Description of drawings

1 is a schematic diagram of the system principle of a multifunctional sperm quality analyzer provided in Embodiment 1;

2 is a schematic structural reference diagram of a multifunctional sperm quality analyzer provided in Embodiment 1;

Fig. 3 is the flow chart of a kind of sperm quality detection method provided in embodiment 2;

Fig. 4 is the flow chart of the liquefaction processing thread of a kind of sperm quality detection method provided in embodiment 2;

FIG. 5 is a flowchart (1) of the classification and disposal steps in the liquefaction processing thread in FIG. 4;

FIG. 6 is a flowchart (2) of the classification and disposal steps in the liquefaction processing thread in FIG. 4;

7 is a flowchart of a reaction processing thread of a sperm quality detection method provided in Embodiment 2;

8 is a flowchart of a microscopic examination processing thread of a sperm quality detection method provided in Embodiment 2;

9 is a flow chart of simultaneously executing a reaction processing thread and a microscopy processing thread for a sperm quality detection method provided in Embodiment 2;

10 is a flowchart of a method for detecting sperm quality in Embodiment 4;

FIG. 11 is a schematic structural diagram of a multifunctional sperm quality analyzer in the fifth embodiment.

Detailed ways

The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments have used associated similar element numbers. In the following embodiments, many details are described so that the present application can be better understood. However, those skilled in the art will readily recognize that some of the features may be omitted under different circumstances, or may be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application from being overwhelmed by excessive description, and for those skilled in the art, these are described in detail. The relevant operations are not necessary, and they can fully understand the relevant operations according to the descriptions in the specification and general technical knowledge in the field.

Additionally, the features, acts, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a necessary order unless otherwise stated, a certain order must be followed.

The serial numbers themselves, such as "first", "second", etc., for the components herein are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "connection" mentioned in this application, unless otherwise specified, include both direct and indirect connections (connections).

The physical and chemical properties of semen usually include semen color, PH value, viscosity, volume, liquefaction time and other items; among them, normal semen should be gray or milky white viscous liquid, if the color of semen is yellow-green or red, it may be caused by the prostate gland. and seminal vesicle non-specific inflammation, germline tuberculosis, tumor or stone; normal semen pH value is 7.2-8.0, semen too acid (if less than 7.0) may be caused by blockage of ejaculatory duct, semen is too alkaline (if more than 7.0) 8.0) may be caused by inflammation of the seminal vesicles, so the change of PH value has a great influence on sperm motility and metabolism; the detection and analysis of semen color is very important for the differential diagnosis of diseases. At the same time, semen morphological testing usually includes items such as sperm motility and sperm count, among which sperm count is an important indicator to measure the ability of testes to produce sperm and the male reproductive tract. In addition, under normal circumstances, the male semen is in a liquefied state when it is just ejected from the body, and will solidify into a jelly or a clot in a short period of time. After 10-30 minutes, the semen will gradually liquefy into a watery liquid , This process is the liquefaction of semen, which is a normal physiological phenomenon. If the semen is still jelly after being discharged from the body for more than 30 minutes, it is a delayed liquefaction; if it still does not liquefy after more than 60 minutes, it is a pathological condition, usually caused by prostatitis, seminal vesiculitis, pathogenic microorganism infection, lack of trace elements, etc.

The sperm quality detection method and multifunctional sperm quality analyzer provided in this application include but are not limited to the automatic liquefaction of semen, the detection of physical and chemical properties such as semen color and pH value, and the morphological detection of sperm count. By integrating the liquefaction mode and the detection mode into one, the liquefaction mode, the detection mode and the compatibility of the two modes can be implemented according to the user's needs or the condition of the semen sample, from the realization of sample liquefaction pretreatment, liquefaction degree detection, sampling, and sample addition. It can not only integrate all or most of the project links required to complete the sperm quality analysis to improve the detection efficiency, but also eliminate the influence of human operation differences by reducing manual intervention or operation. The accuracy of the test results creates conditions.

Example 1

Please refer to FIG. 1 , a multifunctional sperm quality analyzer is provided in this embodiment. The multifunctional sperm quality analyzer mainly includes a sampling mechanism 11 , a controller 14 , a liquefaction processing mechanism 15 , and a reaction processing mechanism 12 and/or a mirror The detection processing mechanisms formed by the detection processing mechanism 13 will be described separately below.

In this embodiment, the multifunctional sperm quality analyzer can inject one or more semen samples at a time, and the user can add the sample information of the semen samples to the preset liquefaction list or detection list by judging the state of the semen samples, so that Which samples need to be liquefied and which samples need to be tested directly.

In this embodiment, the liquefaction processing mechanism 15 is provided with an incubation heating component, and the liquefaction processing mechanism 15 can accept the control of the controller 14, so that the liquefaction processing mechanism 15 can use the incubation heating component to process the information in the sample container corresponding to each sample information in the liquefaction list. The sample is heated and liquefied, such as heating and heat preservation at 37°C, so as to simulate the in vivo environment, so as to speed up the liquefaction speed of the semen sample and realize the preprocessing operation of the sample.

It should be noted that semen liquefaction refers to the jelly-like semen that becomes thinner under the action of fibrinolytic enzymes after 10 to 20 minutes, and then the sperm can move fully; then, the liquefaction of semen samples is usually the same as that of the samples. The viscosity is related to the liquefaction time. The liquefaction time should be less than 30 minutes. If the liquefaction cannot be liquefied or exceeds 1 hour, the motility and survival rate of sperm will be affected, which can also be used as one of the indicators for the detection of sperm quality.

In this embodiment, the sampling mechanism 11 is provided with a sampling needle, and the sampling mechanism 11 can accept the control of the controller 14. First, the sampling can be performed by sampling the samples in the sample container corresponding to each sample information in the liquefaction list. , and perform liquefaction detection; secondly, the sample corresponding to each sample information in the detection list can be aspirated, and the aspirated sample can be added to the reaction vessel and/or to the detection card.

In the present embodiment, the reaction processing mechanism 12 is provided with a reaction container and a first imaging component (such as a camera), then the reaction processing mechanism 12 can use the first imaging component to separately collect the samples in the reaction container before and after the reaction reagent is injected into the reaction container. picture. Usually, the reaction treatment is mainly to detect the pH value of the semen sample. Adding a reaction reagent (PH reagent) to the semen sample can make the semen sample show different colors, so that the pH value can be determined by analyzing the color of the sample.

In this embodiment, the microscopic inspection processing mechanism 13 is provided with a microscope and a second imaging component (such as a camera), then the microscopic inspection processing mechanism 12 can focus the sample on the detection card through the microscope, and use the second imaging component to focus Take an image of the sample on the test card. The purpose of focusing microscopy is to magnify the visible area of the sample and perform morphological detection of semen by microscopic methods. The purpose of taking images is to obtain video and/or images of the focused area, which is convenient for analyzing the number or motility of sperm.

In this embodiment, the controller 14 is in signal connection with the liquefaction processing mechanism 15 , the sampling mechanism 11 , the reaction processing mechanism 12 and the microscopy processing mechanism 13 , and may be an integrated control circuit or a central processing unit. Then, the controller 14 performs time sequence control on the liquefaction processing mechanism 15 , the sampling mechanism 11 , the reaction processing mechanism 12 and the microscopic inspection processing mechanism 13 mainly through a preset detection method. For example: in the liquefaction link, the controller 14 controls the sampling mechanism 11 to absorb the sample in the sample container, and liquefies the result by detecting the viscosity of the sample; in the reaction processing link, the controller 14 controls the reaction processing mechanism 12 to perform the filling of the reaction reagent In the microscopic inspection processing link, the controller 14 controls the microscopic inspection processing mechanism 13 to automatically focus on the detection card, and video and/or focus on the focus area. or image capture.

In order to clearly understand the working principle of the multifunctional sperm quality analyzer, the specific structure of the multifunctional sperm quality analyzer will be described below through a specific embodiment.

Referring to FIG. 2, the multifunctional sperm quality analyzer includes a liquefaction processing mechanism 15, a sampling mechanism 11, an upper card mechanism 16, a reaction processing mechanism 12, and a microscopic inspection processing mechanism 13. Of course, the multifunctional sperm quality analyzer also includes a controller 14 (Fig. 2), the controller is in signal connection with these mechanisms and used to control the execution actions of the corresponding mechanisms. In addition, the first direction, the second direction and the third direction involved in FIG. 2 refer to the three directions naturally formed or the space formed by the three directions when the multifunctional sperm quality analyzer is in the environmental space A Cartesian coordinate system, wherein the first direction is the X-axis direction, the second direction is the Y-axis direction, and the third direction is the Z-axis direction.

In FIG. 2 , the liquefaction processing mechanism 15 is mainly composed of a sample carrying platform, an incubating heating part, a sample injection traveling drive part, and the like. The sample carrying table is used to place a sample container (such as reference sign A) containing a semen sample, and the sample carrying table is provided with a plurality of container holes, and the sample container is placed on the sample carrying table through the container holes. Incubation heating components (such as heating sheets, heating films) are arranged on the sample carrying platform, and are mainly used to heat the sample container, so as to keep the temperature of the semen sample in the sample container within a preset range (such as 20°C-37°C) , thereby facilitating the transformation of the semen sample from a coagulated state to a liquefied state, so that the semen sample is liquefied as soon as possible. The sample injection travel drive is used to drive the sample carrying table to reciprocate in the first direction through the guide rail, and use the speed change effect generated by the sample carrying table during the process of walking and the change of the walking direction, so as to make the sample carrying table vibrate. In the process of transporting the sample container, the carrying table vibrates and mixes the semen samples in the sample container; at the same time, a sampling position is defined on the walking track of the sample injection walking drive assembly, so that the sample carrying table transports the mixed semen sample to the sampling position , the sampling mechanism 11 can draw the semen sample from the sample container.

In FIG. 2 , the sampling mechanism 11 includes a sampling drive, a sampling travel drive and a sampling needle. Among them, the sampling driver is connected with the sampling needle through the liquid pipeline, and after the preparation of the semen sample is completed, the sampling needle can be driven to suck and discharge the semen sample, so as to realize the sample collection and sample adding functions of the sampling mechanism 11; The continuously changing positive and negative pressure effect enables the sampling needle to repeatedly suck and discharge the semen sample, so as to achieve the effect of mixing the semen sample, so that the semen sample can meet the standard of subsequent detection. Wherein, the sampling travel drive includes a vertical guide rail and a horizontal guide rail, the vertical guide rail is slidably arranged on the horizontal guide rail and moves in the second direction, and a sampling needle is fixed on the vertical guide rail and drives the sampling needle to move in the third direction ; When the vertical guide rail moves to the sampling position in the second direction, the sampling needle is driven into the sample container at the sampling position, so that the sampling driver drives the sampling to aspirate the samples in the sample container; when the vertical guide rail is in the second direction When moving in the direction to the sample adding position (such as the first sample adding position of the reaction vessel and the second sample adding position of the detection card), the sampling driver drives the sampling needle to discharge the sucked sample, so as to add the sample to the reaction vessel and the detection card. . In addition, in order to facilitate the detection of the viscosity of the sample, the sampling mechanism 11 also includes a viscosity detector to ensure that the sample sucked by the sampling mechanism 11 meets the relevant detection standards; The internal pressure value of the needle is used to finally realize the viscosity judgment of the semen sample.

In FIG. 2 , the upper card mechanism 16 includes a card magazine and an upper card driving member. Among them, the card compartment is used to store unused detection cards; a detection card incubator can be installed on the card compartment, and the detection card incubator can be used to heat the detection card in the card compartment, so that the temperature of the detection card can be maintained at Within the preset temperature range, it is ensured that after the semen sample is added to the test card, the temperature difference between the two will not adversely affect the semen sample and detection. Among them, the upper card driver is used to move the detection card out of the card compartment and place it in the second sample adding position of the detection card. By providing the function of automatically adding the detection card, the manual operation steps are minimized and conditions are created for improving the detection efficiency.

Of course, in another case, there may be no card loading mechanism, and the user can manually load the card, and directly place the unused test card on the second sample adding position of the test card.

In FIG. 2 , the reaction processing mechanism 12 includes a reaction vessel, a camera, a sample adding pipeline and a sample adding driver. Wherein, the reaction container (such as the reaction cup) is made of transparent material, so that the camera can take pictures of the sample in the reaction container; the reaction container is arranged at the first sample adding position, so that the sampling mechanism 11 can be in the first sample adding position. The semen sample is added into the reaction container, so as to realize the function of containing the semen sample in the reaction container. Wherein, one end of the sample adding pipeline extends into the reaction vessel, and the sample adding driver is connected to the end of the sample adding pipeline far from the reaction vessel; the sample adding driver can drive the sample adding pipeline to add the reaction reagent into the reaction vessel, so that the semen The sample is mixed with the reagents to form a mixed sample.

In FIG. 2, the microscopy processing mechanism 13 includes a detection card conveying mechanism, a microscopy incubation assembly, a microscope and a camera. Wherein, the detection card conveying mechanism is used to transfer the detection card from the second sample adding position to the microscopic inspection position; the microscopic inspection incubation component is arranged on the detection card conveying mechanism and is used for heating the detection card, so that the temperature of the detection card, especially It is to keep the temperature of the semen sample on the detection card within a preset range (such as about 37°C), creating conditions for the effect of microscopic scanning. The microscope is set at the microscope inspection position, and is used to focus the detection card in the microscope inspection position through the automatic focusing component of the objective lens; the camera is set at the eyepiece of the microscope to shoot video or images of the focus area of the microscope, thereby Provides observation conditions for the activity of sperm in semen samples on the detection card.

In some other embodiments, the detection processing mechanism can also be only the reaction processing mechanism 12 or the microscopic inspection processing mechanism 13, so as to reduce the functional items of the multifunctional sperm quality analyzer, reduce the structural volume and occupied space, and achieve compactness Create conditions for type and special type sperm quality analyzers.

Embodiment 2

Based on the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the detection method is mainly applied in the controller shown in FIG. 1 .

In this embodiment, please refer to FIG. 3 , the detection method includes steps S210-S250, which will be described separately below.

Step S210, acquiring a liquefaction list and a detection list. Both the liquefaction list and the detection list here include one or more sample information.

The multifunctional sperm quality analyzer can inject one or more semen samples at one time, some of which are in a jelly state, and these semen samples need to be liquefied first. Then, before the semen sample is injected, the state of the semen sample can be manually identified, the sample information of the semen sample that needs liquefaction is added to the liquefaction list, and the semen sample that does not need liquefaction is added to the detection list, so as to which samples need liquefaction processing, Which samples need to be tested directly provides conditions.

Step S220, triggering and executing the liquefaction processing thread of the sample according to the liquefaction list, obtaining the first information, and adding the sample information after the liquefaction processing ends to the detection list.

Since the semen samples corresponding to each sample information in the liquefaction list need to be liquefied, each sample information can be selected in turn according to the sample order in the table, thereby triggering the execution of the liquefaction processing thread of the sample corresponding to the sample information; the obtained first information It can include liquefaction results including liquefaction time, viscosity value, or fault information including an error in an action in the liquefaction process. After semen liquefaction, it indicates that the semen sample meets the test standards required for subsequent reaction processing and microscopic examination processing, so the sample information after the liquefaction processing needs to be added to the test list.

Of course, if there is no sample information in the liquefaction list, the liquefaction processing thread cannot be triggered. Therefore, in the case that at least one piece of sample information exists in the liquefaction list in step S210, the controller may directly proceed to step S220.

Step S230, triggering a reaction processing thread for executing the sample according to the detection list to obtain third information.

Usually, the semen samples corresponding to each sample information in the detection list need to undergo reaction processing, so each sample information can be selected in turn according to the sample order in the table, thereby triggering the execution of the reaction processing thread of the sample corresponding to the sample information; the obtained third The information includes reaction information including adding reaction reagents, taking pictures of samples, or failure information including an error in an action in reaction processing.

Of course, if there is no sample information in the detection list, the reaction processing thread cannot be triggered. Therefore, in the case that there is at least one sample information in the detection list in step S210, the controller may directly proceed to step S230.

Step S240, triggering and executing the microscopic inspection processing thread of the sample according to the detection list to obtain fourth information.

Usually, the semen samples corresponding to each sample information in the detection list need to undergo microscopic examination processing, so each sample information can be selected in turn according to the sample order in the table, thereby triggering the execution of the microscopic examination processing thread of the sample corresponding to the sample information; the obtained The fourth information includes microscopic inspection information including adjusting the focus of the objective lens, capturing a sample video or image, or including fault information that an action is wrong in the microscopic inspection process.

Of course, if there is no sample information in the detection list, the microscopy processing thread cannot be triggered. Therefore, in the case that there is at least one sample information in the detection list in step S210, the controller may directly proceed to step S240.

Step S250, output the first information, the third information and the fourth information.

It should be noted that, the first information, the third information and the fourth information are all test information or fault information, then the output of these information can be displayed to the user through display, and can also be subjected to subsequent image analysis processing. In addition, since the liquefaction processing thread, the reaction processing thread and the mirror inspection processing thread are executed synchronously, the corresponding information is output only when any of the threads is triggered. For example, if only the reaction processing thread is triggered, then only the output third information.

In this embodiment, the reaction processing thread that triggers the execution of the sample according to the detection list to obtain the third information, and the microscopy processing thread that triggers the execution of the detection list to obtain the fourth information belongs to the detection processing thread that triggers the execution of the sample according to the detection list. , obtain the second information; that is: the detection processing thread that triggers the execution of the sample in the detection list includes the reaction processing thread and the microscopy processing thread, that is to say, the second information is obtained by using the third information and the fourth information; in some embodiments , according to the detection list triggering the execution of the detection processing of the sample, the formation may also be only a reaction processing thread or a microscopic inspection processing thread, and in this case, the second information is the third information or the fourth information.

In the embodiment, the above-mentioned step S220 mainly involves the process of triggering the execution of the liquefaction processing thread of the sample. Referring to FIG. 4 , this step may specifically include S221-S228, which are respectively described below.

Step S211 , select a sample information from the liquefaction list, and trigger the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information. It can be understood that when there is a plurality of sample information in the liquefaction list, the controller can select one sample information in sequence, or can randomly select one sample information.

Step S222, timing step, timing the liquefaction process of the sample to obtain the first time.

Step S223, the liquefaction detection step, detects whether the first time reaches the second time, and when the first time reaches the second time, performs liquefaction degree detection.

In a specific embodiment, the detection result of the degree of liquefaction is the viscosity value of the semen sample. In the liquefaction execution state, a sampling needle is driven to be inserted into the sample container corresponding to the sample information to draw the sample, and the sample is detected by sensing the internal pressure of the sampling needle. viscosity value.

More specifically, referring to FIG. 2 , the controller controls the sampling mechanism 11 to perform some actions, drives the sampling needle to move in the second direction and the third direction through the sampling travel driver, and reaches the sampling position of the sample container A, and drives the sampling needle. Insert into sample container A. Then, the sampling needle is driven by the sampling driver to suck the semen sample in the sample container A, and then the pressure value inside the sampling needle is detected by the pressure sensor set at the tail of the sampling needle, and the controller calculates the viscosity value of the sample. Next, the controller can judge the liquefaction time required for the sample according to the viscosity value of the sample.

Of course, when the controller determines that the viscosity value of the sample is greater than the test standard, it can control the sampling driver to drive the sampling to repeatedly aspirate and dispense the sample in the sample container, thereby rapidly liquefying the sample in the sample container.

Step S224: Execute the classification processing step according to the liquefaction degree detection. If the liquefaction degree detection result is qualified, output the liquefaction result. If the liquefaction degree detection result is unqualified, determine whether the actual liquefaction time of the current sample has reached the third time. When the actual liquefaction time reaches the third time, the liquefaction is stopped and a prompt message indicating that the liquefaction of the current sample is abnormal is output. If the actual liquefaction time of the current sample does not reach the third time, step S222 (the timing step) and step S223 (the liquefaction detection step) are continued. step).

It should be noted that the second time can be a preset detection time point or time period (such as 5min, 15min, 30min, 1h and other time nodes from the start of timing; or from the start of timing, a fixed time period of 10min , 30min, etc., and can be repeated one or more times), the third time should be the preset maximum time allowed for liquefaction (such as 1h), and the second time should be less than the third time.

In step S225, it is judged whether all the actions in the liquefaction execution state are completed, and if so, it goes to step S226, otherwise, it goes to step S227.

It should be noted that the actions in the liquefaction execution state at least include the action of driving the sampling needle, sucking the sample, detecting the internal pressure of the sampling needle, and calculating the viscosity value. Step S226 can be entered after all these actions are completed.

In step S226, since all the actions in the liquefaction execution state are completed, it indicates that the liquefaction process is completed, and liquefaction information of the sample can be generated at this time. The liquefaction information may include the viscosity value of the sample and the liquefaction time of the sample.

In step S227, since the actions in the liquefaction execution state are not all completed, it indicates that some links are faulty, and at this time, the first fault information can be generated. For example, if the action of detecting the internal pressure of the sampling needle cannot be performed, the first fault information that can be generated includes an error in detecting the pressure; if the semen sample cannot be liquefied, a prompt message of abnormal liquefaction is generated.

Step S228, obtaining the first information by using the liquefaction information or the first fault information.

In one embodiment, the above-mentioned step S224 mainly involves performing the classification and treatment process according to the detection of the liquefaction degree. Referring to FIG. 5 , this step specifically includes S2241-S2245, which are described as follows.

Step S2241, compare the liquefaction degree detection result with the preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, execute step S2242; if the liquefaction degree detection result is greater than the preset threshold, execute step S2243.

Step S2242, output the liquefaction result.

Step S2243, determine whether the actual liquefaction time of the current sample has reached the third time, if the actual liquefaction time of the current sample has not reached the third time, execute step S2244; if the actual liquefaction time of the current sample reaches the third time, execute step S2245 .

Step 2244: Extend the second time to obtain a new second time, and compare the first time with the new second time in the continuing step S223 (liquefaction detection step).

Step S2245, outputting prompt information of abnormal liquefaction of the current sample.

In this way, through the control of the second time, the second time is set as multiple time nodes from the timing step, so that the semen samples that have not been liquefied can be tested for the degree of liquefaction multiple times within the third time. The time is set to 5min. When the first time reaches 5min, the liquefaction degree detection is carried out. If the liquefaction degree detection result is unqualified, the second time is extended by 10min, so that the node to obtain a new second time is the 15th minute from the timing step. , when the first time reaches 15min, carry out the liquefaction degree detection; if the liquefaction degree detection result is still unqualified, continue to extend the second time and obtain a new second time, until the second time reaches the third time, carry out the final One-time liquefaction detection.

In another embodiment, in one embodiment, the above-mentioned step S224 mainly involves performing a classification treatment process according to the detection of the liquefaction degree, then referring to FIG.

Step S2246, compare the liquefaction degree detection result with the preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, execute step S2247; if the liquefaction degree detection result is greater than the preset threshold, execute step S2248.

Step S2247, output the liquefaction result.

Step S2248, determine whether the actual liquefaction time of the current sample reaches the third time, if the actual liquefaction time of the current sample does not reach the third time, then execute step S2249; if the actual liquefaction time of the current sample reaches the third time, execute step S22410 .

Step 2249, clear the first time to zero, and start timing from zero in the execution of step S222 (timing step).

Step S22410, outputting prompt information of abnormal liquefaction of the current sample.

In this way, by controlling the second time, the second time is a fixed time period from the timing step, so that the liquefaction degree detection of the semen samples that have not yet been liquefied can be performed multiple times within the third time, which is equivalent to every other The second time (such as 5min, 10min, etc.) is to perform a liquefaction degree detection, and the actual liquefaction time is equivalent to the superposition of multiple second times. When the superimposed second time reaches the third time, the last time Liquefaction degree detection.

In this embodiment, the above-mentioned step S230 mainly involves the process of triggering the reaction processing thread for executing the sample. Referring to FIG. 7 , this step may specifically include S231-S236, which are described as follows.

Step S231, select a sample information from the detection list, and trigger the reaction processing thread to enter the reaction execution state from the idle state. It can be understood that when there are multiple pieces of sample information in the detection list, the controller can select one piece of sample information in sequence, or randomly select one piece of sample information.

Step S232, in the reaction execution state, drive a sampling needle to take samples from the sample container corresponding to the sample information and add the sample to a reaction container, take a first image of the sample in the reaction container, and then inject the sample into the reaction container. React reagents and take a second image of the sample in the reaction vessel.

In a specific embodiment, referring to FIG. 2 , the controller controls the sampling mechanism 11 and the reaction processing mechanism 12 to perform some actions, driving the sampling needle to suck the semen sample in the sample container A through the sampling driving member, and driving the sampling needle through the sampling driving member. It moves in the second direction and the third direction and reaches the first sample adding position of the reaction container, so as to add the drawn semen sample to the reaction container. Then, the controller drives the camera to take a picture of the sample in the reaction container to obtain the first image, and then drives the sample adding driver to inject the reaction reagent (such as pH reagent) into the reaction container, and the semen sample and reaction reagent in the reaction container are to be reacted. After the sufficient reaction, the camera is driven to take pictures of the samples in the reaction vessel again to capture and obtain a second image.

It can be understood that after capturing the first image and the second image, the controller can analyze the pH value of the sample according to these images.

In step S233, it is judged whether all the actions in the reaction execution state are completed.

It should be noted that the actions in the reaction execution state include at least the action of driving the sampling needle, the action of adding the sample, the action of taking pictures for the first time, the action of adding the reaction reagent, and the action of taking pictures for the second time. After all these actions are completed, step S234 can be entered. .

In step S234, since all the actions in the reaction execution state are completed, it indicates that the reaction processing is completed, and reaction information of the sample can be generated at this time. The reaction information may include the first image and the second image, and may also include the pH value of the sample.

In step S235, since the actions in the reaction execution state are not all completed, it indicates that some links are faulty, and at this time, second fault information can be generated. For example, if the action of adding the reaction reagent cannot be performed, the second fault information that may be generated includes an error in adding the reaction reagent.

In step S236, the third information is obtained by using the response information or the second fault information.

In the embodiment, the above-mentioned step S240 mainly involves the process of triggering the execution of the microscopic inspection processing thread of the sample. Referring to FIG. 8 , this step may specifically include S241-S246, which are described as follows.

Step S241, select a sample information from the detection list, and trigger the microscopy processing thread to enter the microscopy execution state from the idle state.

It can be understood that when there are multiple pieces of sample information in the microscopic examination processing list, the controller may select one piece of sample information sequentially, or may randomly select one piece of sample information.

Step S242, in the microscopic inspection execution state, drive a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a detection card, transfer the detection card to the microscopic inspection position, and drive a microscope to focus on the detection card, Shoot the third image and/or the first video of the sample on the test card.

In a specific embodiment, referring to FIG. 2 , the controller controls the sampling mechanism 11 and the microscopic inspection processing mechanism to perform some actions, driving the sampling needle to suck the semen sample in the sample container A through the sampling driving member, and driving the sampling needle through the sampling driving member It moves in the second direction and the third direction and reaches the second sample loading position of the test card (such as a glass slide), so as to add the sucked semen sample to the test card. Then, the detection card is transferred to the microscope inspection position through the inspection card conveying mechanism, and the microscope is driven to adjust the lens so as to focus the inspection card in the microscope inspection position. Next, the camera is driven to capture video and/or images of the focus area on the detection card from the eyepiece of the microscope, so as to capture a third image and a first video.

It can be understood that, after capturing the third image and the first video, the controller can analyze these videos or images to obtain the activity state and quantity of sperm in the semen sample on the detection card.

In step S243, it is judged whether all the actions in the microscopic inspection execution state are completed, and if so, it goes to step S244, otherwise, it goes to step S245.

It should be noted that the actions in the microscopic inspection execution state include at least the action of driving the sampling needle, the action of adding the sample, the action of transferring the detection card, the action of focusing the microscope, and the action of photographing. After all these actions are completed, step S244 can be entered.

In step S244, since all the actions in the microscopic examination execution state are completed, it indicates that the microscopic examination processing is successful, and the microscopic examination information of the sample can be generated at this time. The microscopic examination information may include the third image and the first video, and may also include sperm in the sample. activity status and quantity.

In step S245, since the actions in the microscopic inspection execution state are not all completed, it indicates that some links are faulty, and at this time, third fault information can be generated. For example, if the focusing action of the microscope cannot be performed, the third fault information that can be generated includes a focusing error of the microscope.

In step S246, the fourth information is obtained by using the microscopic inspection information or the third fault information.

In one embodiment, after any task thread is executed, the corresponding task thread is restored from the execution state to the idle state, so as to facilitate restarting the thread. Specifically, the controller restores the liquefaction processing thread to enter the idle state after obtaining the first information, and restores the detection processing thread to enter the idle state after obtaining the second information (for example, after obtaining the third information, the controller restores the reaction processing thread to enter the idle state, and after obtaining the third information, the controller restores the reaction processing thread to enter the idle state, After the fourth message, the mirror inspection processing thread is resumed and enters an idle state).

Of course, when fault information is included in the first information and the second information (ie, the third information and/or the fourth information), the controller may not restore the corresponding task thread immediately, but restore the corresponding task thread after the fault is cleared. The task thread continues to enter the execution state, thereby continuing to perform the actions wherever the failure occurred. Currently, in some cases, the corresponding task thread can be exited after the fault is cleared, so that the task thread is in an idle state.

In one embodiment, for the liquefaction processing thread, the reaction processing thread or the mirroring processing thread, before triggering the execution of any one of the task threads, the idle state/execution state of the task thread is checked, and only the task thread is idle state can only be triggered.

In a specific embodiment, when it is necessary to perform reaction processing and microscopic examination processing on the semen sample at the same time, the execution of the reaction processing thread and the microscopic examination processing thread can be triggered at the same time. For this process, please refer to steps S310-S340 in FIG. 9 , which are described respectively. as follows.

Step S310, check the status of the reaction processing thread and the mirror inspection processing thread.

In step S320, it is judged whether the reaction processing thread and the microscopy processing thread are idle at the same time, and if so, it goes to step S330, otherwise, it goes to step S340.

Step S330, triggering the reaction processing thread to enter the mirror inspection execution state from the idle state, and triggering the mirror inspection processing thread to enter the mirror inspection execution state from the idle state, and then executing the two threads respectively. For the execution process, reference may be made to steps S231-S236 in FIG. 7 and steps S241-S246 in FIG. 8 , which will not be repeated here.

Step S340, if otherwise, interrupt triggering until both threads are idle.

It should be noted that executing the reaction processing thread and the microscopy processing thread at the same time can reduce the waiting time in some cases, because at this time, the sampling needle can add samples to the reaction container and the detection card at the same time, so as to avoid the sampling needle performing suction multiple times. Sample action and sample addition action.

In one embodiment, before acquiring the liquefaction list and the detection list (that is, before step S210 in FIG. 3 ), a response step is further included. The sample information of the sample is classified into the liquefaction list or the detection list. That is to say, when a certain semen sample is injected, the liquefaction state of the semen sample is manually identified and judged. For the semen sample that does not meet the test standard, the corresponding sample information is directly added to the liquefaction list, so that the semen sample is liquefied first. Handling operations.

In one embodiment, after outputting the first information and the second information (ie, the third information and/or the fourth information) (ie, after the step S250 in FIG. 3 ), it further includes a display step and a fault clearing step: (1) in In the display step, the controller displays the first information and the second information (ie, the third information and/or the fourth information) for the user to view; (2) in the fault clearing step, the controller displays the first information, the second information When the information includes corresponding fault information, the fault is cleared according to a preset fault handling mechanism, and the fault handling mechanism may be a preset processing mechanism in the controller, such as program self-check or fault alarm. It can be understood that after the fault is cleared, it is possible to return to the faulty action to continue execution, or to completely exit the corresponding task thread and wait for the next trigger to execute the thread.

Embodiment 3

Based on the multifunctional sperm quality analyzer provided in the first embodiment, this embodiment provides a sperm liquefaction method. The sperm liquefaction detection method is mainly applied in the controller shown in FIG. 1 . In this way, only the The liquefaction processing mechanism 15 and the sampling mechanism 11 are retained to form a method dedicated to liquefying the semen sample; the specific steps can refer to steps 210 and 220 in the second embodiment; the details are as follows:

Step 410, the step of obtaining a liquefaction list, where the liquefaction list includes one or more sample information;

Step 420, triggering the liquefaction step, selecting one of the sample information from the liquefaction list, and triggering the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information;

Step 430, timing step, timing the liquefaction process of the sample to obtain the first time;

Step 440, a liquefaction detection step, to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection;

Step 450: Perform the classification and disposal step according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, output the liquefaction result. If the liquefaction degree detection result is unqualified, determine whether the actual liquefaction time of the current sample has reached the third time. If the actual liquefaction time of the current sample reaches the third time, the liquefaction is stopped. If the actual liquefaction time of the current sample does not reach the third time, continue to perform the timing step and the liquefaction detection step. The second time is less than the third time, and the third The time is the preset maximum time allowed for liquefaction.

Embodiment 4

Based on the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the sperm quality detection method is mainly applied in the controller shown in FIG. 1 .

Referring to FIG. 10 , the detection method in this embodiment includes two parts, one is a UI control part (ie, human-computer interaction control) and a thread control part, the former part is formed with display content on the user interface.

Referring to FIG. 10 , the controller forms some trigger buttons on the user interface, and the user uses the trigger buttons to start the sample testing work of the multifunctional sperm quality analyzer. For each injected sample, the user interface prompts whether the liquefaction operation is required. If the user selects yes, the sample information of the sample is added to the liquefaction list, and if no, the sample information of the sample is added to the detection list.

10 , the controller obtains the liquefaction list and the detection list, triggers the liquefaction processing thread for executing the sample according to the liquefaction list, triggers the reaction processing thread for executing the sample according to the detection list, and triggers the microscopy processing thread for executing the sample according to the detection list. The three task threads can be executed in no particular order and can be triggered synchronously.

In case 1, when selecting a sample information from the liquefaction list and triggering the liquefaction processing thread of the sample, check whether the thread is in an idle state, if so, execute the liquefaction processing thread, otherwise, wait until the thread is idle. For the process of executing the liquefaction processing thread, reference may be made to steps S221-S228 in the second embodiment.

In case 2, when selecting a sample information from the detection list and triggering the reaction processing thread of the sample, check whether the thread is in an idle state, if so, execute the reaction processing thread, otherwise wait until the thread is idle. For the process of executing the liquefaction processing thread, reference may be made to steps S231-S236 in the second embodiment.

In case 3, when selecting a sample information from the detection list and triggering the microscopic inspection processing thread of the sample, check whether the thread is in an idle state, if so, execute the microscopic inspection processing thread, otherwise, wait until the thread is idle. For the process of executing the microscopic inspection processing thread, reference may be made to steps S241-S246 in the second embodiment.

Referring to Figure 10, after each task thread ends, the test information and fault information of the task thread will be obtained. For example, the first information will be obtained after triggering the liquefaction processing thread that executes the sample, and the second information will be obtained after triggering the reaction processing thread that executes the sample. information, the third information will be obtained after triggering the microscopic inspection processing thread that executes the sample, and then the controller can output the information.

Referring to FIG. 10 , when the test information and fault information are output to the user interface, these information are displayed on the user interface, so that the user can check them in time.

Embodiment 5

Based on the detection method provided in Embodiment 2 or Embodiment 3, this embodiment provides a multifunctional sperm quality analyzer.

Please refer to FIG. 11 , the multifunctional sperm quality analyzer 4 includes a memory 41 and a processor 42 . The memory 41 is used for storing a program, and the program may be the program code corresponding to the detection method in the second embodiment, or the program code corresponding to the detection method in the third embodiment.

The processor 42 is connected to the memory 41 for executing the program stored in the memory 41 to implement the detection method. For the functions implemented by the processor 42, reference may be made to the controller in the second embodiment or the third embodiment, which will not be described in detail here.

Those skilled in the art can understand that all or part of the functions of the various methods in the foregoing embodiments may be implemented by means of hardware or by means of computer programs. When all or part of the functions in the above-mentioned embodiments are implemented by means of a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: read-only memory, random access memory, magnetic disk, optical disk, hard disk, etc. The computer executes the program to realize the above-mentioned functions. For example, the program is stored in the memory of the device, and when the program in the memory is executed by the processor, all or part of the above functions can be realized. In addition, when all or part of the functions in the above-mentioned embodiments are realized by means of a computer program, the program can also be stored in a server, another computer, a magnetic disk, an optical disk, a flash disk or a mobile hard disk and other storage media, and saved by downloading or copying All or part of the functions in the above embodiments can be implemented when the program in the memory is executed by the processor.

The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention, and are not intended to limit the present invention. For those skilled in the art to which the present invention pertains, according to the idea of the present invention, several simple deductions, modifications or substitutions can also be made.

Claims (10)

1. a sperm liquefaction method, is characterized in that, comprises: the step of obtaining a liquefaction list, where the liquefaction list includes one or more sample information; Triggering the liquefaction step, selecting one piece of the sample information from the liquefaction list, and triggering the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information; The timing step is to time the liquefaction process of the sample to obtain the first time; The liquefaction detection step is to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection; The classification and disposal step is performed according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, the liquefaction result is output. If the liquefaction degree detection result is unqualified, it is judged whether the actual liquefaction time of the current sample has reached the third time. When the time reaches the third time, stop the liquefaction. If the actual liquefaction time of the current sample does not reach the third time, continue to perform the timing step and the liquefaction detection step. The second time is less than the third time, and the third time is a predetermined time. Set the maximum time allowed to liquefy. 2. a sperm quality detection method, is characterized in that, comprises: obtaining a liquefaction list and a detection list, wherein the liquefaction list and the detection list each include one or more sample information; Trigger the liquefaction processing thread for executing the sample according to the liquefaction list, obtain the first information, and add the sample information after the liquefaction process to the detection list; Execute the detection processing thread of the sample according to the detection list, and obtain second information, where the second information includes the detection information of the sample; Wherein, the liquefaction processing thread that triggers the execution of samples according to the liquefaction list includes: Triggering the liquefaction step, selecting one piece of the sample information from the liquefaction list, and triggering the idle state in the liquefaction processing thread to enter the liquefaction execution state, so as to start the liquefaction process for the sample corresponding to the selected sample information; The timing step is to time the liquefaction process of the sample to obtain the first time; The liquefaction detection step is to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection; The classification and disposal step is performed according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, the liquefaction result is output. If the liquefaction degree detection result is unqualified, it is judged whether the actual liquefaction time of the current sample has reached the third time. If the actual liquefaction time of the current sample does not reach the third time, continue to perform the timing step and the liquefaction detection step, and the second time is less than the third time. Three times, the third time is the preset maximum time allowed for liquefaction; The first information is obtained according to the liquefaction result and/or prompt information. 3. The method according to claim 1 or 2, wherein the classification and treatment step comprises: Compare the liquefaction degree detection result with a preset threshold; If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result; If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time of the current sample reaches the third time; If the actual liquefaction time of the current sample does not reach the third time, the second time is extended to obtain a new second time, and the first time and the new second time are compared in the continuous execution of the liquefaction detection step. 4. The method according to claim 1 or 2, wherein the classification and treatment step comprises: Compare the detection result of liquefaction degree with the preset threshold; If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result; If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time of the current sample reaches the third time; If the liquefaction time of the current sample does not reach the third time, the first time is reset to zero, and the time is started from zero in the continuous execution of the timing step. 5 . The method according to claim 1 or 2 , wherein after stopping the liquefaction, the method further comprises: outputting prompt information indicating that the current sample is abnormal in liquefaction. 6 . 6. The method of claim 1 or 2, wherein the liquefaction degree detection result is the viscosity value of the semen sample. 7. The method of claim 6, wherein the detecting the degree of liquefaction comprises: A sampling needle is driven to be inserted into the sample container corresponding to the sample information to draw the sample, and the viscosity value of the sample is detected by sensing the internal pressure of the sampling needle. 8. The method according to claim 2, wherein the triggering of the detection processing thread for executing the sample according to the detection list, and obtaining the second information comprises: Trigger the reaction processing thread for executing the sample according to the detection list to obtain third information; and/or Trigger the microscopic inspection processing thread of the execution sample according to the detection list to obtain fourth information; The second information is obtained by using the third information and/or the fourth information. 9. A multifunctional sperm quality analyzer, characterized in that it comprises a liquefaction processing mechanism, a sampling mechanism, a detection processing mechanism and a controller; The liquefaction processing mechanism is provided with an incubation heating component, which is used for heating and liquefying the samples in the sample container corresponding to each sample information in the liquefaction list through the incubation heating component; The sampling mechanism is provided with a sampling needle, which is used to aspirate the sample in the sample container through the sampling, and perform liquefaction detection, and to aspirate the sample corresponding to each sample information in the detection list. , and add the drawn sample to the reaction vessel and/or to the test card; The detection and processing mechanism is provided with a reaction container and a first imaging component, which is used to capture images of the samples before and after the injection of the reaction reagent in the reaction container through the first imaging component; and/or the detection and processing mechanism is provided with having a microscope and a second imaging component, used for focusing the sample on the detection card through the microscope, and capturing the sample on the detection card through the second imaging component; The controller is signal-connected with the liquefaction processing mechanism, the sampling mechanism and the detection processing mechanism, and is used for performing the monitoring of the liquefaction processing mechanism, the The sampling mechanism and the detection mechanism perform time sequence control. 10. A computer-readable storage medium, characterized by comprising a program executable by a processor to implement the method according to any one of claims 1-8.

Images