CN113834798B - A multifunctional optical detection and analysis system - Google Patents
A multifunctional optical detection and analysis system Download PDFInfo
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- CN113834798B CN113834798B CN202110858150.6A CN202110858150A CN113834798B CN 113834798 B CN113834798 B CN 113834798B CN 202110858150 A CN202110858150 A CN 202110858150A CN 113834798 B CN113834798 B CN 113834798B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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Abstract
本发明提供了一种多功能光学检测分析系统，包括：光路检测单元、样本处理单元、光路分选单元、信号转换单元和数据处理单元。本发明提供的多功能光学检测分析系统在液相样本只需一次进样时即可实现多种物质的检测，其中涉及不同光学分析方法的检测。同时，能够自动控制液相样本的移取与处理，将原始样本、预处理样本、混合试样按设定程序分配至检测光路中，自动选择对应的光路配置和光学分析方法，实现对同一液相样本内多种不同物质的定量检测，使得多项组合分析更简便、可靠、高效和安全。
The present invention provides a multifunctional optical detection and analysis system, comprising: an optical path detection unit, a sample processing unit, an optical path sorting unit, a signal conversion unit and a data processing unit. The multifunctional optical detection and analysis system provided by the present invention can realize the detection of multiple substances when the liquid sample is injected only once, which involves the detection of different optical analysis methods. At the same time, it can automatically control the removal and processing of liquid samples, distribute the original samples, pre-treated samples, and mixed samples to the detection optical path according to the set program, automatically select the corresponding optical path configuration and optical analysis method, and realize the quantitative detection of multiple different substances in the same liquid sample, making the multi-combination analysis more simple, reliable, efficient and safe.
Description
Technical Field
The invention relates to the technical field of detection and analysis, in particular to a multifunctional optical detection and analysis system.
Background
The optical analysis refers to a method for performing chemical analysis by utilizing optical properties of substances, and common optical analysis comprises fluorescence photometry analysis, transmitted light analysis, scattered light analysis and chemiluminescence analysis, wherein the methods are mature in technology, more in types of analyzable substances and higher in automation level, and are widely applied to the fields of sanitary inspection, environmental and food analysis, pharmaceutical analysis, biochemistry, clinical detection and the like.
However, most of the existing analysis devices are basically provided with an analysis method, such as a full-automatic biochemical analyzer based on transmitted light analysis and a full-automatic luminescence immunoassay analyzer based on chemiluminescence analysis. In practical scientific research production work, detection and analysis of a plurality of different substances are often required to be carried out on the same sample. Due to the limitations of the detection method of the analysis device, even the detection and analysis of the related substances in the same sample can be realized by using a plurality of different analysis devices.
In addition, many detection assays require use of a solution sample that has been pretreated by centrifugation, dissolution, or the like. The process of sample pretreatment requires extensive manual intervention by laboratory personnel, which is a major limiting factor in laboratory automation, and sample exposure during pretreatment is also one of the major biosafety risks in the laboratory.
Disclosure of Invention
In view of the above, the present invention provides a multifunctional optical detection and analysis system to solve the problem of single function of the detection and analysis device.
Based on the above object, the present invention provides a multifunctional optical detection and analysis system, which is characterized by comprising:
The light path detection unit comprises at least two groups of detection light paths, and each group of detection light paths comprises a weak light detection light path and a transmission light detection light path;
the sample processing unit is communicated with the light path detection unit through a pipeline and is used for taking and processing samples;
The optical path sorting unit is used for providing detection light beams for the optical path detection unit and comprises at least two groups of optical path sorting units, and the optical path sorting unit is connected with the optical path detection unit through optical fibers;
the signal conversion unit is electrically connected with the light path detection unit and is used for carrying out signal conversion on the light signal output by the light path detection unit and outputting a digital signal;
The data processing unit is electrically connected with the signal conversion unit and is used for analyzing and processing the digital signals output by the signal conversion unit and outputting analysis data.
Further, the weak light detection light path is used for detecting scattered light or fluorescence of the sample, and the transmission light detection light path is used for detecting transmission light of the sample.
Further, the detection cell in the detection light path comprises a detection area and a capillary, wherein the capillary is arranged in the detection area, the capillary is a wave-shaped capillary, one end of the capillary is an inflow end, and the other end of the capillary is an outflow end.
Further, the output light signal of the weak light detection light path is filtered through a first optical filter and then sent to the photoelectric conversion unit, the first optical filter is assembled in a first round filter wheel, and the first round filter wheel is driven by a first stepping motor.
Further, the sample processing unit comprises an original sample sampling path, a preprocessed sample sampling path and at least one mixed sample sampling path, and samples respectively obtained through the original sample sampling path, the preprocessed sample sampling path and the mixed sample sampling path are detected through the light path detection unit.
Further, the sample processing unit further comprises a cleaning passage and a compressed air passage, the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the cleaning passage, and the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the compressed air passage.
Further, the sample processing unit includes an original sample sampling path and a multiple sample sampling path, and samples obtained through the original sample sampling path and the multiple sample sampling path respectively are detected by the light path detection unit.
Further, the multi-sample sampling path comprises a reaction disc provided with at least two reaction cups, and samples in the reaction cups are detected by the light path detection unit.
Further, the detection light path for detecting the sample in the reaction cup comprises a detection module, and a detection groove matched with the reaction disc is formed in the detection module.
Further, the signal conversion unit includes a photoelectric conversion element that converts the optical signal output by the optical path detection unit into an electrical signal, and an analog-to-digital conversion element that converts the electrical signal into a digital signal.
From the above, it can be seen that the multifunctional optical detection and analysis system provided by the invention can realize detection of various substances when a liquid phase sample is injected only once, wherein detection of different optical analysis methods is involved. The system provided by the invention can automatically control the movement and the processing of the liquid phase sample, distributes the original sample, the pretreated sample and the mixed sample into the detection light path according to the set program, and automatically selects the corresponding light path configuration and the optical analysis method, thereby realizing the quantitative detection of a plurality of different substances in the same liquid phase sample, and ensuring that the multi-item combined analysis is simpler, more convenient, more reliable, more efficient and safer.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a multifunctional optical detection and analysis system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a light path detecting unit according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a sample processing unit according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a detection cell according to an embodiment of the present invention;
FIG. 5 is a schematic diagram showing a sample processing unit according to another embodiment of the present invention;
FIG. 6 is a schematic structural view of a reaction disk according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of an optical path detecting unit according to another embodiment of the present invention;
Fig. 8 is a schematic structural diagram of an optical path sorting unit according to an embodiment of the present invention.
Reference numerals:
1. An optical path detection unit; 11, a first collimator; 12, a first detection pool; 121, inflow end, 122, outflow end, 123, capillary, 124, detection zone, 13, first lens, 141, first filter, 142, first circular filter wheel, 15, first stepper motor, 16, first optical fiber interface, 17, first optical fiber, 18, detection module, 181, detection tank, 182, second collimator, 183, second lens, 184, second optical fiber interface, 185, third optical fiber interface, 186, fourth optical fiber interface, 187, third lens, 188, third filter, 189, third circular filter wheel, 1891, third stepper motor, 2, sample processing unit, 211, first original sample tube, 212, first sampling needle, 213, first rotary sampling valve, 214, first rotary sampling valve, 215, first plunger pump, 216, first quantitative sample ring, 221, first bottle, 222, first rotary sampling valve, 223, first mixing tank, 224, second rotary sampling valve, 2241, second quantitative sample ring, 225, third rotary sampling valve, 226, third rotary sampling ring, third rotary valve, 227, third rotary valve, third mixing tank, 263, second magnetic valve, third rotary valve, first magnetic valve, second magnetic valve, third magnetic valve, cylindrical, the sample, the cylindrical, the sample, the cylindrical, the, the, reagent distribution system, 272, second sampling needle, 273, fourth rotary sampling valve, 2731, fourth quantitative sample ring, 274, third selection valve, 275, third detection cell, 276, third plunger pump, 277, fourth reagent bottle, 278, fourth reagent pump, 281, second original sample tube, 282, third sampling needle, 3, light path sorting unit, 31, light source, 32, fifth optical fiber interface, 33, third collimator, 34, second optical filter, 341, second circular filter wheel, 35, sixth optical fiber interface, 36, second optical fiber, 37, second stepping motor, 4, signal conversion unit, 5, data processing unit.
Detailed Description
The present invention will be further described in detail below with reference to specific embodiments and with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the present invention more apparent.
It should be noted that unless otherwise defined, technical or scientific terms used in the embodiments of the present invention should be given the ordinary meaning as understood by one of ordinary skill in the art to which the present disclosure pertains. The terms "first," "second," and the like, as used in this disclosure, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.
The following describes a multifunctional optical detection and analysis system provided by the invention in detail with reference to the accompanying drawings.
Referring to fig. 1, the present invention provides a multifunctional optical detection and analysis system, including:
The optical path detection unit 1 comprises at least two groups of detection optical paths, wherein each group of detection optical paths comprises a weak light detection optical path and a transmission light detection optical path, and can carry out various optical analyses on a sample, and the two groups of detection optical paths are mutually independent. The light path structures of the two groups of detection light paths can be set to be the same or different, and the detection light paths can be adjusted according to specific detection conditions. The number of the detection light paths is not limited to two groups, and a plurality of groups can be arranged, so that optical detection and analysis can be carried out on samples simultaneously or in a time-sharing manner.
The sample processing unit 2 is communicated with the light path detection unit 1 through a pipeline and is used for moving and processing a sample, sampling and preprocessing can be automatically carried out on the original sample, mixing with other different reagents is carried out, a plurality of mixed samples are automatically prepared, manual intervention is reduced, manual workload is reduced, and sample exposure time is reduced.
The optical path sorting unit 3 is configured to provide a detection beam for the optical path detection unit 1, and includes at least two groups of optical paths, the optical path sorting unit 3 is connected with the optical path detection unit 1 through an optical fiber, a light source of the optical path sorting unit 3 is a wide-spectrum or multi-spectrum light source, and a spectrum light source or a monochromatic light source meeting detection requirements can be output through conversion of an optical element.
And the signal conversion unit 4 is electrically connected with the optical path detection unit 1, and is used for performing signal conversion on the optical signal output by the optical path detection unit 1 to output a digital signal, converting the optical signal through a signal conversion element in the signal conversion unit 4, and converting the optical signal into the digital signal for analysis and processing by the data processing unit 5.
The data processing unit 5 is electrically connected with the signal conversion unit 4, and is used for analyzing and processing the digital signal output by the signal conversion unit 4, and the computer and special software obtain the concentration of the detected object in the detected sample according to the light intensity data-concentration algorithm of the corresponding detected object, so as to complete optical analysis, output analysis data and complete detection of the specified substance in the original sample put into the detection analysis system.
In some embodiments, the weak light detection light path is used to detect scattered light or fluorescence of the sample, and the transmitted light detection light path is used to detect transmitted light of the sample. Referring to fig. 1, the monochromatic light output from the optical path sorting unit 3 is collimated and irradiated to the surface of the sample to be detected, and the monochromatic light is used as excitation light for performing fluorescence photometry analysis on the sample to be detected or is used as an incident light source for transmitted light and scattered light analysis. The fluorescence emitted by the sample to be detected after excitation and the scattered light generated by reflecting the incident light are detected through a weak light detection light path, and the transmitted light formed by the sample to be detected after irradiation of the incident light is detected through a transmitted light detection light path.
In some embodiments, the detection cell in the detection light path includes a detection area 124 and a capillary tube 123, the capillary tube 123 is disposed in the detection area 124, the capillary tube 123 is a wave-shaped capillary tube, one end of the capillary tube 123 is an inflow end 121, and the other end is an outflow end 122.
The first detection cell 12 and the second detection cell 227 in this embodiment are flow detection cells, as shown in fig. 4. The flow detection cell is a flat rectangular quartz glass or transparent polymer material product, and comprises a detection area 124 and a capillary tube 123 therein, wherein the capillary tube 123 is a wave-shaped capillary tube, two ends of the capillary tube 123 are respectively an inflow end 121 of liquid and an outflow end 122 of liquid, and the inflow end 121 and the outflow end 122 are connected in series in a fluid treatment pipeline for inflow and outflow of fluid such as a sample. The detection zone 124 refers to the area within the smallest envelope circle of the capillary 123. The capillary 123 has a uniform inner diameter, and an applicable size of 0.1mm-1.6mm. The ratio of the total length of capillary 123 to its smallest envelope circle, i.e. the area of detection zone 124 is ≡0.3mm/mm 2. Compared with the linear capillary, the wave capillary has larger liquid volume, can store more detection samples, and can effectively improve the optical detection precision due to the increase of the area of the samples for absorbing or reflecting light in the detection process.
The flow detection cell in this embodiment is suitable for detecting various low-viscosity liquid samples, and is not limited to the application mode in this embodiment, but may be used in other devices such as fluorescence analysis, luminescence analysis, scattered light analysis, transmitted light detection, and image analysis.
In some embodiments, referring to fig. 2, the output light signal of the weak light detection light path is filtered by a first optical filter 141 and then sent to the signal conversion unit 4, where the first optical filter 141 is assembled in a first circular filter wheel 142, and the first circular filter wheel 142 is driven by a first stepper motor 15. Specifically, the optical path detecting unit 1 includes two groups of identical detecting optical paths, and the weak light detecting optical path of each group of detecting optical paths sequentially includes a first collimator 11, a first detecting cell 12, a first lens 13 and a first optical filter 141, and incident light emitted by the optical path sorting unit 3 is collimated by the first collimator 11 and then irradiates on a sample surface to be detected of the first detecting cell 12, and light emitted from the first detecting cell 12 is converged and collimated by the first lens 13 and then filtered by the first optical filter 141 and then irradiates on a signal conversion element of the signal conversion unit 4. The first optical filter 141 is mounted on the first circular filter wheel 142, a plurality of groups of optical filters with different wavelengths can be mounted in the first circular filter wheel 142, and the first optical filter 141 with different wavelengths can be selected by rotating the first circular filter wheel 142. The first circular filter wheel 142 is driven to rotate by the first stepping motor 15, the first stepping motor 15 is controlled to operate by a system, the first circular filter wheel 142 is driven to rotate according to the optical path parameter setting corresponding to the sample to be detected in the detection pool, and the first optical filter 141 installed in the first circular filter wheel 142 is selected to be in a detection optical path so as to complete optical detection of a weak light detection optical path. The transmitted light detection light path comprises in order a first collimator 11, a first detection cell 12 and a first optical fiber interface 16. The incident light irradiates the first detection cell 12 after passing through the first collimator 11, and forms a transmission light on the other side of the first detection cell 12, where the transmission light passes through the first optical fiber interface 16, and then passes through the first optical fiber 17 to be transmitted to the signal conversion unit 4, and the first optical fiber interface 16 in this embodiment is a microscope optical fiber interface.
The two groups of detection light paths in the light path detection unit 1 can realize the characteristic optical data detection of two different samples. In this embodiment, the feature optical data detection includes fluorescence detection, transmitted light detection and scattered light detection, and the detection light path may be adjusted according to the actual detection requirement to realize detection of other feature optical data, which is not limited to the three feature optical data presented in this embodiment.
In some embodiments, the sample processing unit 2 includes an original sample sampling path, a preprocessed sample sampling path, and at least one mixed sample sampling path, and samples respectively obtained through the original sample sampling path, the preprocessed sample sampling path, and the mixed sample sampling path are detected by the optical path detecting unit 1. The sample processing unit 2 can detect the original sample and detect the pretreated sample and the mixed sample, so that detection of various substances can be realized. One mixed sample sampling path corresponds to one mixed sample, and the mixed sample sampling path may be added according to practical situations, and is not limited to one mixed sample sampling path in the present embodiment.
In some embodiments, the sample processing unit 2 further comprises a purge path and a compressed air path, the pre-treatment sample sampling path and the mixed sample sampling path being in communication with the purge path, respectively, and the pre-treatment sample sampling path and the mixed sample sampling path being in communication with the compressed air path, respectively. The cleaning passage can clean the mixing pool for preprocessing samples and mixing samples, and the compressed air passage can fully mix the samples in the mixing pool by conveying compressed air, so that the sample detection precision is improved.
Referring to fig. 3, the sample processing unit 2 includes an original sample sampling path, a pre-processed sample sampling path, two mixed sample sampling paths, a purge path, and a compressed air path. The raw sample sampling path sequentially comprises a first raw sample tube 211, a first sampling needle 212, a first rotary sampling valve 213, a first selection valve 214, a first detection cell 12 and a first plunger pump 215, wherein the first rotary sampling valve 213 is rotated to a sampling position, the raw sample sampling needle 212 is driven by a motor to enter the raw sample tube 211, the raw sample sampling path sucks raw sample from the raw sample tube 211, one part of the sucked raw sample enters the first detection cell 12 for detection, the other part of the raw sample is filled into a first quantitative sample ring 216 in the first rotary sampling valve 213, and the sampling is completed, and the motor-driven sampling needle 212 is removed from the sample tube. After the original sample in the first detection tank 12 is detected, the first plunger pump 215 pushes the first detection tank 12 to be filled with pure water, the first detection tank 12 is cleaned, and the cleaned waste water is pushed by the first plunger pump 215 to be discharged from a waste discharge port of the first selection valve 214 for the next suction detection of the sample.
The pretreatment sample sampling path sequentially comprises a first reagent bottle 221, a first reagent pump 222, a first rotary sampling valve 213, a first mixing tank 223, a second rotary sampling valve 224, a third rotary sampling valve 225, a second selection valve 226, a second detection tank 227 and a second plunger pump 228, specifically, the first rotary sampling valve 213 rotates to a distribution position, the first reagent pump 222 discharges pretreatment liquid sucked from the first reagent bottle 221, the raw sample filled in the first quantitative sample ring 216 is pushed to be sequentially injected into the first mixing tank 223, the pretreatment liquid and the raw sample are mixed into the pretreatment sample, the second rotary sampling valve 224 and the third rotary sampling valve 225 rotate to a sampling position, the pretreatment sample sampling path sucks the pretreatment sample from the first mixing tank 223, a part of the sucked pretreatment sample enters the second detection tank 227 for detection, and the other part of the pretreatment sample fills the second quantitative sample ring 2241 and the third quantitative sample ring 2251 in the second rotary sampling valve 224 and the third rotary sampling valve 225, so that the pretreatment sample is sampled. When the pretreated sample in the second detection cell 227 is detected, pure water is injected into the second detection cell 227 to clean by pushing the second plunger pump 228, and the cleaned waste water is pushed by the second plunger pump 228 to be discharged from the second selection valve 226, so that the second detection cell 227 is flushed, and the sample is sucked and detected next time.
The sample sampling path of the first mixed sample sequentially includes a second reagent bottle 231, a second reagent pump 232, a second rotary sampling valve 224, a second mixing cell 233, a second selector valve 226, a second detection cell 227, and a second plunger pump 228, and the sample sampling path of the second mixed sample sequentially includes a third reagent bottle 241, a third reagent pump 242, a third rotary sampling valve 225, a third mixing cell 243, a second selector valve 226, a second detection cell 227, and a second plunger pump 228. The second rotary sampling valve 224 and the third rotary sampling valve 225 are rotated to the dispensing position, the second reagent pump 232 discharges the reagent sucked from the second reagent bottle 231, the pretreated specimen filled in the second quantitative sample ring 2241 is pushed to be sequentially injected into the second mixing tank 233, the third reagent pump 242 discharges the reagent sucked from the third reagent bottle 241, the pretreated specimen filled in the third quantitative sample ring 2251 is pushed to be sequentially injected into the third mixing tank 243, and the reagent and the pretreated specimen are uniformly mixed to be a mixed specimen. The mixed sample in the second mixing tank 233 and the third mixing tank 243 is sucked into the second detection tank 227 for detection by the second plunger pump 228 through the second selector valve 226 in sequence through the pipeline connected to the second selector valve 226.
In this embodiment, the pretreated sample in the first mixing tank 223, the mixed samples in the second mixing tank 233 and the third mixing tank 243 are also respectively communicated with the first selector valve 214 through a pipeline, and are sucked and filled into the first detection tank 12 via the first plunger pump 215 for detection.
The air compression path includes an air compression pump 251 and first, second, and third air solenoid valves 252, 253, 254 in the path. The first air solenoid valve 252 is pulsed on a plurality of times, and a small amount of compressed air is pulsed into the first mixing tank 223 by the air compression pump 251 to thoroughly mix the pre-processed sample. The second air solenoid valve 253 and the third air solenoid valve 254 are pulse-connected a plurality of times, and a small amount of compressed air is pulse-pumped into the second mixing tank 233 and the third mixing tank 243 by the air compression pump 251, so that the mixed samples are fully and uniformly mixed.
The cleaning path includes a cleaning liquid reagent bottle 261, a first cleaning solenoid valve 262, a cleaning pump 263, a fifth cleaning solenoid valve 268, a second cleaning solenoid valve 264, a third cleaning solenoid valve 265, a fourth cleaning solenoid valve 266, and a waste pump 267, respectively, the second cleaning solenoid valve 264, the third cleaning solenoid valve 265, and the fourth cleaning solenoid valve 266, which are connected to the waste pump 267, are connected, the waste pump 267 is started to empty the residual liquid in the first mixing tank 223, the second mixing tank 233, and the third mixing tank 243, then the fifth solenoid valve 268 and the cleaning solenoid valves connected to the mixing tanks are connected, cleaning liquid in the cleaning liquid reagent bottle 261 is injected into the mixing tanks via the cleaning pump 263, and then the mixing tanks are emptied, thereby completing one-time cleaning. The first solenoid valve 262 is turned on, pure water is injected into each mixing tank through the cleaning pump 263, and each mixing tank is emptied one after another, so that one rinsing is completed. Each mixing tank after being cleaned and rinsed can be used for mixing the next sample.
The inner cavity of the mixing tank in the embodiment is cylindrical or elliptic cylindrical, and each mixing tank at least comprises 4 pipeline connection ports, namely a sample moving port, a sample taking port, an air inlet port and an emptying/cleaning port. Except for the connection port, the mixing tank in this embodiment is of a fully closed structure.
In some embodiments, the sample processing unit 2 includes an original sample sampling path and a multiple sample sampling path, and samples obtained through the original sample sampling path and the multiple sample sampling path respectively are detected by the optical path detection unit 1. Specifically, referring to fig. 5, the original sample sampling path is unchanged, and the pre-processed sample sampling path and the mixed sample sampling path are replaced with a multi-sample sampling path, which sequentially includes a fourth reagent bottle 277, a fourth reagent pump 278, a fourth rotary sampling valve 273, a second sampling needle 272, and a reaction disk 271. The raw sample sampling path in this embodiment includes, in order, a second raw sample tube 281, a third sampling needle 282, a fourth rotary sampling valve 273, a third selector valve 274, a third test cell 275, and a third plunger pump 276. The fourth rotary sampling valve 273 is rotated to the sampling position, the motor drives the third sampling needle 282 into the second raw sample tube 281, the raw sample is sucked from the second raw sample tube 281, a part of the sucked raw sample enters the third detection cell 275 for detection, and the other part is filled into the fourth quantitative sample ring 2731 in the fourth rotary sampling valve 273, so that the sampling is completed, and the motor drives the third sampling needle 282 to move out of the second raw sample tube 281. After the detection of the original sample in the third detection pool 275 is completed, pure water is poured into the third plunger pump 276 for cleaning, and the cleaned waste water is pushed by the third plunger pump 276 to be discharged from a waste discharge port of the third selection valve 274, so that the third detection pool 275 is flushed, and the next sample to be detected is sucked and detected.
The fourth rotary sampling valve 273 is rotated to the dispensing position, the fourth reagent pump 278 discharges the pretreatment liquid sucked from the fourth reagent bottle 277, the raw samples filled in the fourth quantitative sample ring 2731 are pushed to be filled together into the reaction disk 271 to be mixed into the pretreated sample, and the pretreated sample and the reagent are uniformly mixed to prepare a mixed sample. The reaction disk 271 is rotated by an external motor, and the pretreated sample and the mixed sample after completion of the reaction are rotated to complete the detection of the characteristic light in the optical path detecting module 18 composed of optical elements, and the optical path detecting module 18 is included in the optical path detecting unit 1.
In some embodiments, referring to fig. 6, the multi-sample sampling path includes a reaction disk 271 provided with at least two reaction cups 2711, and samples within the reaction cups 2711 are detected by the optical path detecting unit 1. Specifically, the reaction disk 271 includes a plurality of reaction cups 2711 circumferentially disposed at equidistant intervals on the edge of the reaction disk 271, each reaction cup 2711 also functioning as a detection cup. The reaction disk 271 cooperates with the sample loading system 2712 and the reagent dispensing system 2713 to perform multiple sampling and dispensing of the pretreated samples, and a plurality of mixed samples are formed by sequentially loading different reagents into the reaction cup 2711 for mixed reaction. The sample and the pretreatment liquid filled from the fourth rotary sampling valve 273 are stored in one of the reaction cuvettes 2711 and mixed to form a pretreated sample, the sample-filling system 2712 samples and redistributes the pretreated sample from the reaction cuvette 2711 to the other reaction cuvettes 2711, and the reagent-dispensing system 2713 injects different reagents into the reaction cuvettes, respectively, to form a plurality of mixed samples.
In some embodiments, the detection light path for detecting the sample in the reaction cup 2711 includes a detection module 18, and a detection groove 181 matched with the reaction disk 271 is formed inside the detection module 18. Referring to fig. 7, the optical path detecting unit 1 includes two sets of detection optical paths, wherein the detection optical paths of one set are used for detecting the third detection cell 275, and the detection manner is the same as that described above, and the description thereof is omitted. The other set of detection light paths is used for detecting the sample in the reaction cup 2711 of the reaction disk 271. The detection module 18 is placed below the reaction disk 271 such that the detection cups 2711 are positioned in the detection slots 181 of the detection module 18, and the width of the detection slots 181 is greater than the thickness of the detection cups 2711 in the reaction disk 271, so as to ensure that each detection cup 2711 in the reaction disk 271 can move and be detected smoothly in the detection slots 181. The detection module 18 includes a second collimator 182, a second lens 183, a second optical fiber interface 184, and a third optical fiber interface 185, where the second collimator 182, the second lens 183, and the second optical fiber interface 184 are included in the weak light detection light path, and the third optical fiber interface 185 is included in the transmitted light detection light path. The incident light from the optical path sorting unit 3 is collimated by the second collimator 182 and then irradiates on the surface of a sample in one of the detection cups 2711 of the reaction disc 271, scattered light generated by the reflection of the sample or fluorescence generated by the excitation of the sample is converged and collimated by the second lens 183 and then enters the second optical fiber interface 184, and then enters the third optical filter 188 after being converged and collimated by the fourth optical fiber interface 186 and the third lens 187, and the light emitted by the third optical filter 188 is irradiated on the signal conversion unit 4 for signal conversion. The third filter 188 is fitted in a third circular filter wheel 189, and the third circular filter wheel 189 is driven by a third stepper motor 1891. The combined use of the detection cell and the detection cup in the embodiment is suitable for detection requirements of combination of multistage treatment and different optical analysis methods, and compared with the use of a single detection cell, more reagent mixed samples can be conveniently prepared, so that more substances can be quantitatively detected more rapidly.
In some embodiments, the sorting light path comprises a second filter 34 for outputting a monochromatic light source, the second filter 34 being fitted in a second circular filter wheel 341, the second circular filter wheel 341 being driven by a second stepper motor 37. Referring to fig. 8, the optical path sorting unit 3 includes two-component optical paths, which are identical in configuration in the present embodiment. Each component optical path includes a light source 31, a fifth fiber interface 32, a third collimator 33, a second filter 34, and a sixth fiber interface 35. The light source 31 is a wide-spectrum or multi-spectrum light source, and wide-spectrum light emitted by the light source 31 is transmitted to the third collimator 33 through the fifth optical fiber interface 32 and the second optical fiber 36, is divided into two paths of wide-spectrum light output, and is collimated into two parallel light beams through the third collimator 33. And then two beams of monochromatic light are formed by filtering through the second optical filter 34, the two beams of monochromatic light respectively irradiate the light receiving surfaces of the two symmetrically arranged sixth optical fiber interfaces 35, and monochromatic light which can be transmitted by optical fibers is converged and output in the sixth optical fiber interfaces 35. The wavelength of the monochromatic light is determined by the second optical filter 34, the second optical filter 34 is assembled in the second circular filter wheel 341, a plurality of groups of narrow-band optical filters can be assembled in the second circular filter wheel 341, and the second circular filter wheel 341 is driven to rotate by the second stepping motor 37. The optical path sorting unit 3 can output two paths of monochromatic light with different wavelengths simultaneously, and the two paths of the monochromatic light are combined into a group. According to the system instruction, the optical path sorting unit 3 can output a plurality of groups of specified monochromatic light groups with different wavelengths in a time-sharing manner.
In some embodiments, the signal conversion unit 4 includes a photoelectric conversion element that converts the optical signal output by the optical path detection unit 1 into an electrical signal, and an analog-to-digital conversion element that converts the electrical signal into a digital signal. In this embodiment, the photoelectric conversion element includes a photomultiplier tube, which is a photodetector for weak light such as fluorescence and scattered light, and a photodiode, which is a photodetector for transmitted light. The optical signal is converted into current by a photoelectric detector, then is amplified by the signal, and is converted into light intensity data by an analog-to-digital conversion element A/D, so that the detection of the characteristic optical data of the sample to be detected is realized. The photomultiplier and the photodiode can be replaced by CCD, CMOS and other image detection devices, so that image analysis and spectrum analysis of a sample to be detected can be realized.
The working flow of the multifunctional optical detection and analysis system provided by the invention is described below:
Workflow 1:
The original sample is put into a multifunctional optical detection analysis system, and is distributed and processed into 4 samples by a sample processing unit 2, wherein the samples are respectively an original sample, a preprocessed sample and two mixed samples, the structure of the sample processing unit 2 is shown in fig. 3, and the light path detection unit 1 is shown in fig. 2. As shown in fig. 3, the original sample and the pretreated sample are respectively filled into the first detection cell 12 and the second detection cell 227 for detection, after the detection is completed, the first detection cell 12 and the second detection cell 227 are respectively emptied and cleaned, and then the two mixed samples are detected, and the detection method is the same as above.
The characteristic light detection of the 4 samples is carried out through the light path detection unit 1, the light path sorting unit 3 outputs corresponding characteristic monochromatic light groups according to the system instruction according to the excitation light characteristic wavelength of the sample to be detected, the characteristic monochromatic light groups are transmitted to the light path detection unit 1, the sample to be detected is excited by the characteristic monochromatic light to generate characteristic emission light, stray light is filtered by a corresponding optical filter selected according to the system instruction in the light path detection unit 1, and then the stray light is detected through a weak light detection light path, and the process is a fluorescence detection process.
According to the characteristic wavelength of the absorption light of the sample to be detected, the light path sorting unit 3 outputs a corresponding characteristic monochromatic light group according to a system instruction, and transmits the corresponding characteristic monochromatic light group to the light path detection unit 1, wherein the characteristic monochromatic light is partially absorbed by the sample to be detected. The unabsorbed transmitted light is transmitted to the signal conversion unit 4 by the first optical fiber interface 16 in the optical path detection unit 1 through the first optical fiber 17, and the transmitted light intensity is in direct proportion to or inverse proportion to the concentration of the sample to be detected, and the process is a transmitted light detection process, and the transmitted light is detected through the transmitted light detection optical path.
According to the characteristic wavelength of the absorption light of the sample to be detected, the light path sorting unit 3 outputs a corresponding characteristic monochromatic light group according to a system instruction, and transmits the corresponding characteristic monochromatic light group to the light path detection unit 1, wherein the characteristic monochromatic light is scattered by the part of the sample to be detected. According to the system instruction, a corresponding scattered light filter set is selected in the light path detection unit 1, and the scattered light enters the signal conversion unit 4 and is detected, wherein the process is a scattered light detection process and is detected through a weak light detection light path.
The three optical signals are converted into digital light intensity data which can be identified, stored and called by computer software through the signal conversion unit 4. The light intensity data is further analyzed and calculated through the data processing unit 5, the concentration of the detected object in the sample to be detected is obtained, the optical analysis is completed, the analysis data is output, and the detection of the specified substance in the original sample is completed.
To sum up, in this process, after the original samples are put in, they are allocated to be processed into 4 samples. Sequentially filling the sample into 2 independent detection pools for detection, detecting at least 4 characteristic light data through 2 groups of independent light paths, and quantitatively detecting at least 4 different substances in the original sample according to a corresponding optical analysis method.
Workflow 2:
The present flow differs from the workflow 1 in that the optical path detection unit 1 and the sample processing unit 2. The optical path detection unit 1 refers to fig. 7, and the sample processing unit refers to fig. 5 and 6. The original sample is put into the sample processing unit 2, and the original sample and the pretreatment liquid are sampled and distributed, and the original sample and the pretreatment liquid are handled by the reaction disk 271 when forming the pretreated sample and preparing the mixed sample, unlike the workflow 1. Multiple mixed samples are formed in the cuvette 2711 by the cooperation of the sample application system 2712 and the reagent distribution system 2713. After the preparation of the mixed sample, the reaction disk 271 is rotated into the detection module 18 for optical detection. The reaction disk 271 is placed in the test cell 181, and the test of different mixed samples is completed by aligning different reaction cups 2711 into the test cell 181 by rotation, and the test of the pretreated sample is also completed in the reaction cups 2711. The original sample is then tested in the third test cell 275, the test principle being the same as that of workflow 1.
For different samples to be detected, three different characteristic lights of fluorescence, transmission light and scattered light can be detected through the light path detection unit 1 respectively, corresponding characteristic optical signals are obtained through the signal conversion unit 4, and finally optical analysis is completed through the data processing unit 5, and optical data are output.
The flow adopts an analysis device combining a detection pool and a detection cup, can carry out multistage distribution and treatment on samples, is suitable for combining multistage treatment with different optical analysis methods, and combines and detects specific samples of a plurality of substances.
It will be appreciated by persons skilled in the art that the foregoing discussion of any embodiment is merely exemplary and is not intended to imply that the scope of the disclosure, including the claims, is limited to these examples, that the steps may be implemented in any order and that many other variations of the different aspects of the invention described above are present, and that combinations of features of the above embodiments or in different embodiments may be implemented within the spirit of the invention, which are not provided in detail for the sake of brevity.
The embodiments of the invention are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, improvement, etc. of the present invention should be included in the scope of the present invention.
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| CN101782575B (en) * | 2010-02-10 | 2012-12-05 | 郑州博赛生物技术股份有限公司 | Inner ring and outer ring double flow line structure for fully-automatic immunity analyzer |
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