CN111781344A - A fluorescence immunoassay analyzer - Google Patents

Abstract

The invention relates to the technical field of detection, and discloses a fluorescence immunoassay analyzer, which comprises an incubation disc structure, a photographing identification mechanism and a scanning card kicking mechanism, wherein the photographing identification mechanism and the scanning card kicking mechanism are positioned above the incubation disc.

Description

A fluorescence immunoassay analyzer

technical field

The invention relates to the technical field of detection, in particular to a fluorescence immunoassay analyzer.

Background technique

The main functions of the fluorescence immunoassay analyzer are: quantitatively detect the contents of various proteins, antigens and other items in human serum, plasma, whole blood or urine in vitro, which are used for the diagnosis, prevention, treatment and health monitoring of clinical diseases. Provide true and accurate data basis.

At present, most fluorescent immunoassay analyzers on the market are divided into several categories: one is single-channel instruments, which are relatively small in size and flexible in application scenarios, but can only test one sample at a time, with low test throughput and lack of Kick cards and devices designed to accommodate waste cards are prone to contamination. The other type is a semi-automatic multi-channel instrument, which can complete multi-sample and multi-item incubation tests at the same time, but often uses the track-in-card-plus-disc incubation scheme. This kind of instrument needs to manually insert the reagent card into the track, and then insert the reagent card into the track manually. The motor is driven to enter the top of the incubation tray, and another motor is needed to drive the kicking mechanism to kick the reagent card onto the incubation tray. The card feeding speed is slow, the failure rate is high, and the cost is high; in addition, the incubation tray lacks a limit device, and the reagent is stuck on the tray. The inner part can move axially. Due to centrifugation and collision during rotation, the sample on the reagent card has the risk of overflow, which will cause biological contamination.

In recent years, in order to better meet the requirements of point-of-care testing (POCT), realize the purpose of fast, simple and accurate detection, and reduce the influence of human error on the detection results, the automatic fluorescence immunoassay analyzer has emerged as the times require.

Existing fully automatic instruments, which can complete automatic card loading and automatic sampling, all need to be equipped with a complex automatic sample loading system, which has a high failure rate and a high risk of biological contamination. It is difficult to detect whole blood samples and peripheral whole blood samples, and the instrument is bulky and has limited application scenarios. It can only be used in laboratory departments of medium and large hospitals. However, in this scenario, compared with fully automatic luminescence and biochemical instruments, the detection speed and Accuracy is not an advantage. In addition, many instruments on the market need to use an ID card or IFID card to pre-enter the reagent calibration information to update the database before testing a new batch of reagent items before testing. If the operator accidentally loses the ID card or forgets to insert it before use card, it will cause a waste of time and use costs.

SUMMARY OF THE INVENTION

In order to overcome the above problems, the present invention provides a fluorescent immunoassay with many incubation positions, fast card insertion speed, small instrument size, low production cost, fast detection speed, automatic identification of reagent card calibration information, simple structure, safety and stability instrument.

A fluorescence immunoassay analyzer includes a housing and a frame, the housing includes a bayonet slot and a bayonet slot, and further includes:

an incubation plate mechanism, comprising a rotating plate, a driving structure and a constant temperature heating structure, the driving structure is connected with the rotating plate, and the constant temperature heating structure is installed on the rotating plate;

a photographing identification mechanism, including a camera obscura structure and a photographing structure, the photographing structure is located in the camera obscura structure, and the photographing identification mechanism is located above the incubation plate mechanism;

Scanning and kicking mechanism, including a conveying device, a kicking device and an optical structure that are cooperatively connected with the conveying device, and the positions of the conveying device, the kicking device and the optical structure are all corresponding to the position of the reagent card

Correspondence mentioned here: refers to the information that the optical structure can scan on the reagent card, and that the kicking device can contact the reagent card.

The door closing mechanism is installed on the casing and is located at one side of the card slot.

The door closing mechanism blocks the entrance of the reagent card, which can prevent the danger or contamination caused by the rotation of the disk body.

Preferably, the photographing identification mechanism and the scanning card kicking mechanism are located in two different stations. The reagent card inserted into the incubation plate is first rotated to the bottom of the photo-recognition mechanism, the photo of the reagent card is taken by the photo-taking structure, and the items and calibration information are entered through the barcode on the identification reagent card. When the reagent card is scanned, kicked, or incubated, the latter reagent card can be photographed and identified. The two processes are closely connected, which shortens the detection time and enables fast and high-throughput detection.

Preferably, the reagent card rotates 3/4 of a circle from the insertion of the card slot until the detection is completed and kicked out from the kick slot, which shortens the detection time, and the slot is always vacant, which can ensure the card insertion efficiency.

Preferably, the turntable includes a plurality of radially distributed pressure plates, the pressure plates are arranged at intervals, and a slot is formed between the two pressure plates for fixing the inserted reagent card.

The incubation plate of the present invention can have multiple card slots, into which multiple reagent cards are inserted, so as to realize simultaneous incubation and detection of more than 25 reagent cards and ensure the detection throughput.

Preferably, the card slot is provided with a mounting hole, and a positioning bead for assisting the fixing of the reagent card is installed in the mounting hole, and the positioning bead includes a housing with a mounting hole, a spring mounted at the bottom of the mounting hole, and a spring located at the bottom of the mounting hole. The steel ball at the upper end and the cover plate installed on the casing, the cover plate covers part of the steel ball, and the shape of the steel ball matches the bottom shape of the reagent card. When the reagent card is inserted into the bottom of the card slot, the steel ball is embedded in the reagent card. bottom. The auxiliary fixation effect of the positioning beads on the reagent card prevents the reagent card from being thrown out due to centrifugal force when the turntable rotates, thereby increasing the stability and fault tolerance of the instrument.

Optionally, an installation hole is provided on the card slot, and a spring, a steel ball at the upper end of the spring and a cover plate installed on the reaction plate are installed in the installation hole, the cover plate covers part of the steel ball, and the steel ball The shape of the reagent card matches the shape of the bottom of the reagent card to prevent the reagent card from being thrown out due to centrifugal force when the turntable rotates.

Preferably, the driving mechanism includes a rotating shaft assembly, a first timing belt and a first motor, one end of the rotating shaft assembly is connected to the axis of the turntable, the other end is connected to a first timing belt, and the first timing belt is sleeved It is arranged on the output shaft of the first motor, and the first motor drives the rotating shaft assembly to rotate through the first synchronous belt, thereby driving the turntable to rotate, and the first motor is installed on the frame.

Preferably, the rotating shaft assembly includes a bearing, a pulley shaft, a bearing sleeve, and a timing pulley, the bearing is sleeved outside the pulley shaft, the bearing is located in the bearing sleeve, and is connected with the bearing sleeve. The cylinders are arranged coaxially, and the synchronous pulley is located at the lower end of the bearing sleeve and is connected with the synchronous belt.

Preferably, the rotating shaft assembly further includes a positioning structure, the positioning structure is connected with the bearing sleeve, and has a disk joint and a positioning pin, and the disk joint and the positioning pin are both matched and connected with the turntable, so that the turntable can be improved. The positioning accuracy, the disk joint and positioning pin can be one or more

Preferably, the constant temperature heating structure includes a heating plate support, a heating plate, a temperature sensor, and a heating sheet. One end of the heating plate support is fixed on the frame, and the other end is connected to the bottom surface of the heating plate. The heating sheet and The temperature sensors are installed on the heating plate. The temperature sensors are used to sense the temperature change of the heating plate and feed it back to the system. The temperature is kept constant through system control. The heat generated by the heating plate is transferred to the turntable through the heating plate. , to keep the turntable at a constant temperature.

Preferably, the heating sheet is annular, and the heating sheet is installed on the lower surface of the heating plate.

Preferably, an annular groove is formed on the lower surface of the heating plate, and the heating sheet is fitted into the annular groove.

Optionally, the heating sheet is directly attached to the lower surface of the heating plate.

The above-mentioned constant temperature heating structure can improve the utilization efficiency of heat, save energy and facilitate temperature control.

Preferably, the heating plate is supported by a heating plate bracket, and the upper surface is horizontally aligned with the lower surface of the turntable, and the vertical direction is coincident.

Preferably, the incubation tray mechanism further includes a card insertion detection device, the card insertion detection device is installed on the lower surface of the heating plate, the position of the center line coincides with the center line of the turntable, and is aligned with the card insertion port for detecting whether there is a Insert the reagent card.

Preferably, the incubation disk mechanism further includes an encoding disk, the encoding disk includes a positioning block and a first reset block, and the encoding disk is located under the turntable and rotates synchronously with the turntable.

Preferably, the encoder disc is located at the bottom end of the rotating shaft assembly, and is driven to rotate by a bearing.

Preferably, the incubation plate further includes a detection sensor, the detection sensor is installed on the rack, and includes a first position sensor and a second position sensor, the first position sensor and the positioning block of the encoding plate are in the same position A horizontal plane, the second position sensor and the reset block of the encoder disk are on the same horizontal plane.

The positioning block of the encoding disk rotates to block the first position sensor, which can feed back the position information of the turntable; the first reset block is installed under the encoding disk to block the second position sensor, which can Feedback whether the turntable has reached the initial position.

Preferably, the photographing identification mechanism includes a camera obscura structure and a shooting structure, the camera obscura structure has an upper cover of the camera obscura, the shooting structure includes a camera and a white light source, the camera is located at the center of the lower surface of the upper cover of the camera obscura, the white light The light source is located on the lower surface of the upper cover of the dark box and is directly opposite to the left and right sides of the center line of the inserted reagent card. The inner surface of the dark box is provided with a matte coating so that the inner surface is not reflective.

The camera is located in the center of the lower surface of the upper cover of the box body, which can prevent the reflected light from the surface of the reagent card from affecting the photographing effect of the camera.

Preferably, the white light source is preferably an LED light source.

Preferably, the dark box structure can completely cover a whole reagent card.

The setting of the photo identification structure adopts the optical structure to identify the calibration information on the reagent card. Different batches of reagent cards can be mixed and used, without using an external storage device (such as an ID card or IFID card) to update the database, which simplifies the operation and can avoid waste.

Preferably, the scanning kicking mechanism includes an optical structure, a conveying device and a kicking device, the conveying device includes a slider, a linear slide, a mounting plate, a second timing belt, and a second motor, and the optical structure is fixed on the On the sliding block, the sliding block is installed on the linear sliding rail, the bottom surface of the mounting plate is 90° with the side surface, and is fixed on the rack, the linear sliding rail is installed on the bottom surface of the mounting plate, the The second timing belt and the second motor are mounted on the side of the mounting plate

Preferably, the kicking device includes a kicking plate, and the kicking plate is L-shaped and installed at the tail of the optical structure.

Preferably, the optical structure further includes a clamping structure, the clamping structure is clamped on one side of the second synchronous belt, and the second motor drives the second synchronous belt to rotate, thereby driving the optical structure to reciprocate.

Preferably, the optical structure further includes a trigger mechanism, the trigger mechanism includes a photoelectric switch and a second reset blocking piece, the photoelectric switch is arranged on the mounting plate, and the second reset blocking piece is arranged on the slider, and its installation position is the same as that of the photoelectric switch. The switch is adapted to judge whether the optical seat is in the initial position.

Optionally, the trigger mechanism includes two photoelectric switches and a second reset stopper, the photoelectric switches are all arranged on the mounting plate, the second reset stopper is arranged on the slider, and its installation position is the same as that of the two reset plates. The photoelectric switch is adapted to judge whether the optical seat is in the initial position and whether the kicking action is completed correctly.

Preferably, the motion trajectory of the optical structure is parallel to the reagent card, and the stroke covers the entire reagent card.

Preferably, the fluorescence immunoassay analyzer further comprises a waste card bin, and the waste card bin receives the reagent card dropped from the kicking bayonet.

Preferably, the door closing mechanism is installed on the shell of the fluorescence immunoassay analyzer, and includes a movable door, a motor, and a position sensor. The door closing reset block is adapted to the position sensor, and the movable door is driven to rotate by the motor to open or close the bayonet slot. Ensure that the reagent card will not be disturbed and polluted by the outside world during incubation and detection.

The door closing reset blocking piece of the present invention is adapted to the position sensor: it means that when the door closing reset blocking piece covers the position sensor, the movable door is closed.

Preferably, the first position sensor, the second position sensor, the position sensor and the card insertion detection device may preferably be an optical sensor, an opposite-beam optical sensor or a reflective optical sensor, as long as the detection purpose can be achieved.

Beneficial effects of the present invention:

(1) The photo identification mechanism uses an optical structure to identify the calibration information on the reagent card, and uses the system photo information for input. Different batches of reagent cards can be mixed. There is no need to use an external storage device to update the database, simplifying operations and avoiding waste. (2) The photo identification mechanism and the scanning card kicking mechanism are located in two different workstations, which can simultaneously perform two operations of photo identification and scanning kick card on different reagent cards. The two operations are closely connected, saving operation time. Compared with In one station, two operations must be performed successively, which means that the detection time is faster, especially when the number of reagent cards is large, two stations show obvious advantages, and the detection time will be greatly shortened, which is very suitable for high-throughput detection. .

(3) The present invention includes a card slot, a photo-recognition station, a scanning kick station and a kick card slot, the reagent card is inserted from the card slot, and is kicked out of the chuck when the detection is completed, and only rotates 3/4 circles in total, The reagent card after the detection is completed will be quickly removed to ensure that there will be no reagent card at the card insertion station, which can realize continuous card insertion and ensure the detection throughput. The card insertion method is manual in-line insertion. After the incubation plate automatically rotates to the next position to continue to insert the card, the card insertion efficiency is high, the detection time is shortened, and the rapid and high-throughput detection is realized through reasonable settings.

(4) Since the incubation plate pressing plates are arranged at intervals, the card slots are naturally formed, so a large number of reagent cards can be accommodated.

(5) The incubation plate adopts a constant temperature heating structure with a heating plate and a ring-shaped heating plate, which can evenly incubate the reagent cards on the rotating plate, and the heating plate and the heating plate are tightly connected with the rotating plate, which can make the whole incubation plate mechanism compact. Both the photo-recognition structure and the scanning and kicking mechanism are closely arranged above the incubation plate mechanism, and integrate the photographing and identification, scanning and kicking. , suitable for miniaturized instruments.

(6) In the present invention, a door closing mechanism is also provided, which automatically closes and blocks the card insertion hole when the disc body rotates, so that neither the reagent card nor the operator's fingers can be inserted, so as to avoid the occurrence of danger or pollution.

(7) In the present invention, an external waste card bin is adopted, and the detected waste card will fall into the waste card bin to avoid pollution, and at the same time, it is very convenient to replace.

Description of drawings

Figure 1 is a schematic diagram of the internal structure of the fluorescence immunoassay analyzer;

Fig. 2 is the structural representation of incubation plate mechanism;

Fig. 3 is the structural representation of photographing identification mechanism;

Fig. 4 is the structural representation of scanning kicking mechanism;

Fig. 5 is the external schematic diagram of fluorescence immunoassay analyzer;

Fig. 6 is the structural representation of constant temperature heating structure;

Fig. 7 is the structural representation of fluorescence immunoassay analyzer;

Figure 8 is a schematic diagram of the structure of the positioning bead.

Among them: 1- Incubation plate mechanism; 2- Photo recognition mechanism; 3- Scan kick card mechanism; 4- Door closing mechanism.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

The invention provides a fluorescence immune analyzer.

A fluorescence immunoassay analyzer, as shown in Figures 1 and 5, includes an incubation plate mechanism 1, a photographing identification mechanism 2, a scanning and kicking mechanism 3, and a door closing mechanism 4.

As shown in FIG. 2 , the incubation plate mechanism 1 includes a turntable 11 , a driving structure 12 and a constant temperature heating structure 13 . The driving structure 12 is connected to the axis of the turntable 11 , and the driving structure 12 drives the turntable 11 to rotate. The constant temperature heating structure 13 is installed under the turntable 11 .

As shown in FIG. 3 , the photographing identification mechanism 2 includes a camera obscura structure 21 and a photographing structure (not shown in the figure), the photographing structure is located in the camera obscura and faces the incubation plate, and the photographing identification mechanism is located in the Incubate above the plate mechanism.

As shown in FIG. 4 , the scanning kicking mechanism 3 includes a kicking device 31 , an optical structure 32 and a conveying device 33 . The kicking device 31 is installed on the optical structure 32 , and the kicking device 31 sends the reagent The card 5 is kicked out of the incubation plate mechanism 1 from the kicking bayonet, the optical structure 32 is fixedly connected with the conveying device 33, the conveying device 33 drives the optical structure 32 to reciprocate, and the scanning kicking mechanism 31 above the incubation tray mechanism 1 .

As shown in FIG. 7 , the door closing mechanism 4 is installed on the casing, on the side of the card slot, and includes a movable door, a motor, and a position sensor. The movable door is installed at the top of the motor, and the door is closed to reset the shutter. The motor is driven to rotate, and when the door-closing reset block covers the position sensor, the movable door is closed to ensure that the reagent card will not be disturbed and polluted by the outside during incubation and detection.

The photographing identification mechanism 2 and the scanning card kicking mechanism 3 are located in two different stations. When the drive structure 12 drives the turntable 11 to rotate, the reagent card 5 inserted into the incubation plate first rotates under the photographing identification mechanism 2, and the photographing structure takes a picture of the reagent card 5 and records the barcode on the reagent card through the system identification. Project and scaling information.

As shown in FIG. 2 , the turntable 11 includes radially distributed pressure plates 111 , and the pressure plates are arranged at intervals to form card slots 112 for fixing the inserted reagent cards 5 .

The incubation tray of the present invention may have multiple card slots into which multiple reagent cards are inserted. In this embodiment, the number of card slots is 25.

As shown in FIG. 5 and FIG. 8 , the card slot 112 is provided with a mounting hole 113 , and a positioning bead for assisting the fixing of the reagent card is installed in the mounting hole 113 . The spring 1132 installed at the bottom of the mounting hole, the steel ball 1133 at the upper end of the spring, and the cover plate 1134 installed on the housing. The cover plate 1134 covers part of the steel ball 1133. The shape of the steel ball 1133 matches the bottom shape of the reagent card. When the card is inserted into the bottom of the card slot, the steel ball is embedded in the bottom of the reagent card.

The shape of the steel ball matches the shape of the bottom of the reagent card: when the reagent card is inserted into the bottom of the card slot, the steel ball is embedded in the groove of the reagent card to ensure that the reagent card will not shake on the turntable.

As shown in FIG. 1 , the driving mechanism 12 includes a rotating shaft assembly 125 , a first timing belt 122 and a first motor 121 . The upper end of the rotating shaft assembly is connected to the axis of the turntable 11 , and the lower end is connected to the first timing belt 122 , the first motor 121 drives the rotating shaft assembly 125 to rotate through the first synchronous belt 122, thereby driving the turntable to rotate, and the first motor 121 is installed on the frame.

The rotating shaft assembly 125 is mounted on the frame through bearings.

As shown in FIG. 2 and FIG. 6 , the constant temperature heating structure 13 includes a heating plate bracket 133, a heating plate 132, a temperature sensor, and a heating sheet 131. The lower end of the heating plate bracket 133 is fixed on the frame, and the upper end is connected to the bottom surface of the heating plate 132. A temperature sensor 134 is also installed on the bottom surface of the heating plate 132. The temperature sensor 134 is used to sense the temperature change of the heating plate and feed it back to the system to keep the temperature constant through the system control. The heating plate 132 is transferred to the turntable 11 to keep the turntable 11 at a constant temperature, thereby realizing the constant temperature incubation of the reagent card.

The upper surface of the heating plate 132 is horizontally aligned with the lower surface of the turntable 11, and the vertical direction is coincident.

The heating plate 132 and the heating sheet 131 are annular.

As shown in FIG. 2 , the incubation disk mechanism further includes an encoding disk including a positioning block 123 and a first reset block 124 , the encoding disk is located under the turntable and rotates synchronously with the turntable.

The encoder disk is located at the bottom end of the rotating shaft assembly 125 .

The incubation tray 1 further includes a detection sensor 14, the detection sensor 14 is installed on the rack, and includes a first position sensor 141 and a second position sensor 142, the first position sensor 141 and the positioning block of the encoding disk The plate 123 is in the same horizontal plane, and the second position sensor 142 is in the same horizontal plane as the reset blocking plate 124 of the encoder disk.

In this embodiment, the first position sensor and the second position sensor are both facing photoelectric sensors.

The positioning block 123 of the encoder disk rotates to block the first position sensor 141, which can feed back the position information of the turntable; the first reset block 124 is installed under the encoder disk to block the second position The sensor 142 can feed back whether the turntable has reached the initial position.

The incubation tray mechanism further includes a card insertion detection device 15, the card insertion detection device is installed on the lower surface of the heating plate 132, and the position of the center line passes through the center of the turntable 11 and is aligned with the insertion slot for detecting whether A reagent card 5 is inserted.

The photo-recognition mechanism 2 includes a camera obscura structure 21 and a shooting structure, the shooting structure includes a camera and a white light source, the camera is located at the center of the lower surface of the upper cover of the camera obscura, and the white light source is located on the lower surface of the camera cover and is facing On the left and right sides of the center line of the inserted reagent card, the inner surface of the dark box has a matte coating.

The camera is located in the center of the lower surface of the upper cover of the box body, which can prevent the reflected light from the surface of the reagent card from affecting the photographing effect of the camera.

The white light source is an LED light source.

The camera obscura structure 21 can completely cover a whole reagent card.

The scanning and kicking mechanism 3 includes a kicking device 31, an optical structure 32 and a conveying device 33. The conveying device 33 includes a slider (not shown in the figure), a linear sliding rail 312, a mounting plate 34, and a second synchronous belt. 313. The second motor 311, the optical structure 32 is fixed on the slider, the slider is mounted on the linear slide rail 312, the mounting plate 34 is fixed on the frame, and the linear slide rail 312 is mounted on the On the bottom surface of the mounting plate 34 , the second timing belt 313 and the second motor 311 are mounted on the side of the mounting plate 34 .

As shown in FIG. 1 , in this embodiment, the installation plate 34 includes an installation side plate 341 and an installation bottom plate 342 , the installation side plate 341 is fixed on the installation bottom plate 342 , the installation bottom plate 342 is fixedly connected to the frame by means of a bracket, and the photo identification mechanism 2 is fixed on the On the installation bottom plate 342 , the scanning kicking mechanism 3 is fixed on the installation side plate 341 .

As shown in FIG. 4 , the kicking device includes a kicking plate 31 , and the kicking plate is L-shaped and installed at the tail of the optical structure 32 .

The optical structure 32 further includes a clamping structure 321, the clamping structure 321 is clamped on one side of the second timing belt 313, and the second motor 311 drives the second timing belt 313 to rotate, thereby driving the optical structure 32 reciprocating motion.

The optical structure 32 further includes a second reset block. In this embodiment, the reset block is mounted on the miscellaneous clamping structure 321, and the mounting side plate 341 of the mounting plate 34 also includes a photoelectric switch 322, which is located at the One end of the side plate 341 of the mounting plate is close to the center of the incubation tray. When the second reset block cuts off the detection signal, the optical structure is in the initial position.

The movement track of the optical structure 32 is parallel to the reagent card, and the stroke covers the entire reagent card.

As shown in FIG. 7 , the fluorescence immunoassay analyzer further includes a waste card bin 6, and the waste card bin receives the reagent card dropped from the kicking bayonet.

The working process of the fluorescence immunoassay analyzer provided by the present invention is as follows: after receiving the working signal, the reagent card is inserted into the turntable 11, the door closing mechanism 4 is closed, and the inserted reagent card is fixed on the platen 111 and the positioning beads under the auxiliary action In the card slot 112, the first motor 121 drives the turntable 111 to rotate, and the reagent card rotates to the bottom of the photo-recognition mechanism 2. The photo-taking structure takes a photo of the reagent card, and records the items and calibration information by identifying the barcode on the reagent card. Rotate to the bottom of the scanning card kicking mechanism 3, and use the optical structure 32 to identify the calibration information on the reagent card. After scanning, the card kicking plate kicks the reagent card out of the turntable and falls into the waste card bin 6, and then the turntable rotates back to the initial stage. position, ready for the next cycle.

Example 2

The difference between this embodiment and Embodiment 1 lies in the installation hole. The installation hole is installed with a spring, a steel ball at the upper end of the spring, and a cover plate installed on the reaction plate. The cover plate covers part of the steel balls, and the steel balls The shape matches the bottom shape of the reagent card.

Example 3

The difference between this embodiment and Embodiment 1 is that there are two photoelectric switches 322 , and they are both disposed on the mounting plate, and the two photoelectric switches correspond to the second reset block, and the second reset block It is arranged on the slider, wherein the photoelectric switch is arranged at the initial position of the optical mechanism 32 (ie, the zero position close to the inner side of the turntable 11 ). Another photoelectric switch is arranged at the position where the optical structure 32 completes the kicking action, and is used in conjunction with the second reset block to determine whether the optical structure 32 correctly completes the kicking action.

Claims (10)

1. The utility model provides a fluorescence immunoassay appearance, includes shell and frame, the shell includes the bayonet socket and plays the bayonet socket, its characterized in that still includes:

the incubation disc mechanism comprises a rotary disc, a driving structure and a constant-temperature heating structure, wherein the driving structure is connected with the rotary disc, the constant-temperature heating structure is arranged on the rotary disc, the rotary disc comprises radially distributed pressing plates, and the pressing plates are arranged at intervals to form clamping grooves for fixing inserted reagent cards;

the photographing identification mechanism comprises a dark box structure and a photographing structure, the photographing structure is positioned in the dark box structure, and the photographing identification mechanism is positioned above the incubation disc mechanism;

the scanning card kicking mechanism comprises a conveying device, a card kicking device and an optical structure, wherein the card kicking device is connected with the conveying device in a matched mode, and the positions of the conveying device, the card kicking device and the optical structure correspond to the positions of reagent cards.

2. The fluoroimmunoassay analyzer of claim 1, wherein the photograph recognition mechanism and the scanning kicker mechanism are located at two different stations.

3. The fluoroimmunoassay analyzer of any one of claim 1, wherein the slot has a mounting hole, and a positioning bead for assisting in fixing the reagent card is mounted in the mounting hole, the positioning bead comprises a housing having a mounting hole, a spring mounted at the bottom of the mounting hole, a steel ball positioned at the upper end of the spring, and a cover plate mounted on the housing, and the cover plate covers part of the steel ball.

4. The fluoroimmunoassay analyzer of any one of claim 3, wherein the driving mechanism comprises a rotating shaft assembly, a first synchronous belt and a first motor, the upper end of the rotating shaft assembly is connected with the axis of the rotating disc, the lower end of the rotating shaft assembly is connected with the first synchronous belt, the first motor drives the rotating shaft assembly to rotate through the first synchronous belt, so as to drive the rotating disc to rotate, and the first motor is mounted on the frame.

5. The fluoroimmunoassay analyzer of any one of claims 1 to 4, wherein the constant temperature heating structure comprises a heating plate, a heating plate support, a heating plate and a heating plate temperature sensor, one end of the heating plate support is fixed on the frame, the other end of the heating plate support is connected with the lower bottom surface of the heating plate, the heating plate is further provided with the heating plate and the temperature sensor, and the heating plate is annular.

6. The fluoroimmunoassay analyzer of any one of claim 5, wherein the incubation disc mechanism further comprises an encoding disc, a detection sensor and a card insertion detection device, the encoding disc comprises a positioning stop and a first reset stop, and the encoding disc is positioned below the rotating disc and rotates synchronously with the rotating disc; the detection sensor is arranged on the rack and comprises a first position sensor and a second position sensor, the first position sensor and the positioning blocking piece are positioned on the same horizontal plane, and the second position sensor and the reset blocking piece of the coding disc are positioned on the same horizontal plane; the card inserting detection device is installed on the lower surface of the heating plate, the central line position coincides with the central line of the incubation disc, and the card inserting detection device is aligned to the card inserting opening and used for detecting whether a reagent card is inserted.

7. The fluoroimmunoassay analyzer of any one of claims 1 to 4 or 6, wherein the photograph identification mechanism comprises a camera structure and a photographing structure, the camera structure has a camera top cover, the photographing structure comprises a camera and a white light source, the camera is located at a central position of a lower surface of the camera top cover, the white light source is located at the lower surface of the camera top cover and faces to the left and right sides of the center line of the inserted reagent card, and the inner surface of the camera is provided with a matte coating.

8. The fluoroimmunoassay analyzer of any one of claims 1 to 4, wherein the kick card scanning mechanism comprises an optical structure, a conveying device and a kick card device, wherein the conveying device comprises a slide block, a linear slide rail, a mounting plate, a second synchronous belt and a second motor, the optical structure is fixed on the slide block, the slide block is mounted on the linear slide rail, the mounting plate is fixed on the machine frame, the linear slide rail is mounted on the bottom surface of the mounting plate, and the second synchronous belt and the second motor are mounted on the side surface of the mounting plate; the optical structure further comprises a clamping structure, the clamping structure is clamped on one side of the second synchronous belt, and the second motor drives the second synchronous belt to rotate, so that the optical structure is driven to reciprocate.

9. The fluoroimmunoassay analyzer of any one of claims 1 to 4, further comprising a door closing mechanism, wherein the door closing mechanism is mounted on the housing of the fluoroimmunoassay analyzer and comprises a movable door, a motor and a position sensor, the movable door is provided with a door closing reset stop piece and is mounted at the top end of the motor, the door closing reset stop piece is matched with the position sensor, and the movable door is driven by the motor to rotate so as to open or close the card slot.

10. The fluoroimmunoassay analyzer of any one of claims 1 to 4 or 6, further comprising a waste card receptacle for receiving a reagent card dropped from the kick-off port.

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