CN109294915B - Slide combination for animal cell experiment - Google Patents
Slide combination for animal cell experiment Download PDFInfo
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- CN109294915B CN109294915B CN201811198759.XA CN201811198759A CN109294915B CN 109294915 B CN109294915 B CN 109294915B CN 201811198759 A CN201811198759 A CN 201811198759A CN 109294915 B CN109294915 B CN 109294915B
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- slide
- groove
- animal cell
- cell experiments
- combination
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- 238000002474 experimental method Methods 0.000 title claims abstract description 28
- 210000004102 animal cell Anatomy 0.000 title claims abstract description 24
- 238000005192 partition Methods 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 23
- 239000011521 glass Substances 0.000 claims description 13
- 230000008859 change Effects 0.000 claims description 7
- 230000006872 improvement Effects 0.000 claims description 5
- 230000032798 delamination Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 3
- 238000005498 polishing Methods 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 10
- 238000004043 dyeing Methods 0.000 abstract description 8
- 238000012258 culturing Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 230000008569 process Effects 0.000 description 8
- 108010039918 Polylysine Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000012832 cell culture technique Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention is suitable for the technical field of medical treatment and experimental operation, provides a slide combination for animal cell experiments, and aims to solve the problems of waste and low efficiency of dyeing and rinsing in animal cell experiments, and the slide combination comprises a slide, an improved six-hole plate and a toothed clamp, and is characterized in that a slide main body is cylindrical, a circular table-shaped groove is formed in the center, side walls are inclined from outside to inside, four symbols are distributed around the groove at equal intervals, three "+" symbols are distributed around the groove, one P "symbol is distributed around the groove, thickened semicircular protrusions are arranged at symmetrical positions at two ends of the slide, and the toothed clamp consists of clamping arms, springs, a central shaft and toothed protrusions; the improved six-hole plate is characterized in that the plate body is a hollow cuboid, a vertical partition plate is arranged in the middle of the plate body to separate left and right side areas, a horizontal baffle is arranged between the plate wall and the vertical partition plate, 12 fan-shaped slide clamping grooves are arranged in the horizontal baffle, and 6 slides can be simultaneously accommodated. The method is mainly used for culturing various animal cells, efficiently dyeing and rinsing, and carrying out subsequent experiments or observations.
Description
Technical Field
The invention belongs to the technical field of medical treatment and experimental operation, and particularly relates to a slide combination for animal cell experiments.
Background
In bioscience experiments, both basic and clinical experiments, living cell culture techniques are used. Good staining and rinsing are critical to the subsequent experimental steps. At present, when an experimenter cultures living animal cells, the experimenter selects the smallest culture dish, the culture dish is not proportional to the size of the cells, a lot of dye liquor is wasted in the dyeing process, and the cells are washed in the rinsing process, so that loss is caused. Is not easy to operate in the process and requires specialized training. Therefore, a dyeing and rinsing device special for animal cell experiments is urgently needed to solve the problems.
Disclosure of Invention
The invention aims to solve the problems of saving dye liquor, reducing cell number loss in the process and ensuring the accuracy of experiments on the premise of meeting the requirement of high-quality dyeing of animal cells.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the utility model provides a slide combination for animal cell experiments, includes slide, improvement six orifice plates and dentate clamp, slide main part is cylindrical, and there is round platform form first recess in the center, and the lateral wall is the slide that the outside down goes up to interior oblique side wall can change into second recess and third recess according to the cell size difference, has four signs around the recess, and three "+" sign, a "P" sign, and slide both ends symmetry position has the semicircular projection of thickening, dentate clamp comprises arm, spring, axis, dentate projection, improvement six orifice plates, the plate body is hollow cuboid, and the centre sets up the baffle and separates both sides region, and the plate body sets up horizontal baffle with the baffle, and 12 fan-shaped slide draw-in grooves altogether are put to horizontal baffle.
Further preferably, the slide has a diameter of 30mm.
Further preferably, the slide has a thickness of 3mm.
Further preferably, the depths of the first groove, the second groove and the third groove are half of the thickness of the glass slide, and are 1.5mm.
Further preferably, the first groove, the second groove and the third groove are three specifications, and the diameters of the bottoms are 5mm,10mm and 15mm respectively.
Further preferably, the bottoms of the first groove, the second groove and the third groove are provided with cell delamination prevention (polylysine/resin).
Further preferably, the included angle between the inclined side walls of the truncated cone-shaped first groove, the second groove and the third groove and the bottom surface is 60 degrees.
Further preferably, the "+" and "P" signs described in the further preferred embodiments change positions accordingly based on the change in groove aperture.
Further preferably, the thickness of the slide clamping groove is consistent with that of the slide, and is 3mm.
In a further preferred mode, the toothed clamp is made of plastic integrally, and the toothed protrusions are made of soft rubber.
Further preferably, the tail ends of the toothed clamping arms are provided with anti-slip sand-polishing grains.
Compared with the prior art, the invention has the following advantages:
firstly, the front of the slide has grooves with three specifications, the diameters of the grooves are 5mm,10mm and 15mm, the grooves are suitable for animal cell experiments with different sizes and different densities, and the application range is wide.
Secondly, the groove area on the front surface of the glass slide is a cell fixing area, and the area of the cell fixing area is smaller, so that the dosage of cell dye liquor can be greatly reduced, and the experimental cost is greatly saved.
Thirdly, the side wall of the groove on the front surface of the glass slide is a diagonal side wall from bottom to top, and the physical structure prevents cells from being released out of the groove in the dyeing and rinsing processes.
Fourth, the slide front has four signs, three "+" signs, one "p" sign, and p-shaped sign is used for judging slide positive and negative, is convenient for keep slide front upwards all the time in the experimental process, and three +and p are located the four corners of recess, are convenient for fix a position the cell field of vision under the microscope fast.
Fifthly, each hole of the improved six-hole plate is internally provided with a baffle plate, so that a certain distance is kept between the slide and the bottom of the hole plate, and the slide is more stable and is convenient to clamp by a toothed clamp.
Sixth, the slide can be directly dyed, rinsed and dried in the improved six-hole plate without additional operation and equipment, thus saving time and cost.
Seventh, six slides can be operated simultaneously to this six orifice plates of improvement, very big improvement efficiency. And the glass slides are arranged in sequence, so that confusion is avoided, and accuracy is improved.
Eighth, there is the cell to prevent delamination in the slide groove, and this slide combination can make the slide right side up all the time in the experimental process, has prevented the drop and losing of cell in the experiment effectively, has guaranteed the required cell number of experiment, has increased the success rate of experiment.
Drawings
FIG. 1 is a top view of a slide;
FIG. 2 is a side view of a slide;
FIG. 3 is a top view of a modified six-well plate placement slide;
FIG. 4 is a top view of a modified six-well plate without slides;
FIG. 5 is a side view of a modified six-well plate;
FIG. 6 is a schematic view of a toothed clip;
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
As shown in fig. 1 to 6, the slide combination for animal cell experiments shown in this embodiment includes a slide 1, an improved six-hole plate 9 and a toothed clamp 13, the slide 1 has a cylindrical main body, a first groove 2 with a circular truncated cone shape in the center, a slide with a sidewall which is inclined from the outside down to the inside up and can be replaced by a second groove 3 and a third groove 4 according to different cell sizes, four symbols are arranged around the grooves, three "+" symbols 6, one "P" symbol 7, thickened semicircular protrusions 5 are arranged at symmetrical positions at two ends of the slide 1, the toothed clamp 13 is composed of a clamping arm 14, a spring 15, a center shaft 16 and a toothed protrusion 17, the improved six-hole plate 9 has a hollow cuboid, a partition plate 11 is arranged in the middle to separate two side areas, a horizontal baffle 10 is arranged between the plate and the partition plate, and 12 fan-shaped slide clamping grooves 12 are arranged in the horizontal baffle 10.
In this embodiment, specifically, the diameter of the glass slide 1 is 30mm, which is convenient for clamping and is not easy to be damaged.
In this embodiment, specifically, the thickness of the slide 1 is 3mm, and the clamping is convenient.
In this embodiment, specifically, the depths of the first groove 2, the second groove 3 and the third groove 4 are half of the thickness of the glass slide, which is 1.5mm, and the thickness can be directly used for observation under a microscope or further operation.
In this embodiment, specifically, the first groove 2, the second groove 3 and the third groove 4 have three specifications, and the bottom diameters are 5mm,10mm and 15mm respectively, which can be used for animal cells with different sizes, different densities and different types.
In this embodiment, specifically, the bottoms of the first groove 2, the second groove 3, and the third groove 4 are provided with cell delamination preventing layers (polylysine/resin) for adhering cells and preventing the cells from falling off.
In this embodiment, specifically, the included angle between the inclined side wall 8 and the bottom surface of the truncated cone-shaped groove is 60 degrees, and the physical structure prevents cells from rushing out of the groove in the dyeing and rinsing processes.
In this embodiment, specifically, the "+" symbol and the "P" symbol 7 in the further preferred manner change positions according to the change of the aperture of the groove, so that the symbol does not affect the groove and the experimental field.
In this embodiment, specifically, the thickness of the slide clamping groove 12 is consistent with that of the slide, and is 3mm, so that the slide can be well clamped, and the slide is prevented from being damaged during movement.
In this embodiment, specifically, the toothed clip 13 is made of plastic material, the toothed protrusion 17 is made of soft rubber material, and the toothed clip is as lightweight and economical as possible under the premise of satisfying the mechanical stability.
In this embodiment, specifically, the end of the toothed clamping arm 14 has anti-skid sand-polishing grains.
The application method of the invention comprises the steps of firstly selecting a slide with proper groove caliber according to the size of the selected biological cells, putting the biological tissue cells into a circular groove, putting the slide 1 into a slide clamping groove 12 of the improved six-hole plate 9, then dyeing, directly rinsing the slide by using a syringe during rinsing, directly flowing the rinsing liquid into a cavity of the improved six-hole plate 9 without moving the slide, causing little loss of the cells, airing and then moving by using a toothed clamp, and avoiding polluting the front surface of the slide.
The device is suitable for basic and clinical experiments of various biosciences. By using the technical scheme of the invention or under the inspired by the technical scheme of the invention, a similar technical scheme is designed by a person skilled in the art, so that the technical effects are achieved, and the technical effects fall into the protection scope of the invention.
Claims (11)
1. The utility model provides a slide combination for animal cell experiments, includes slide (1), improvement six orifice plates (9) and cusp clamp (13), its characterized in that: the glass slide is characterized in that the glass slide (1) is cylindrical in main body, a first groove (2) in the shape of a circular table is arranged in the center, the side wall (8) is a glass slide which is externally and internally provided with a bevel side wall and can be replaced with a second groove (3) and a third groove (4) according to different cell sizes, four symbols, three "+" symbols (6) and one P symbol (7) are arranged around the grooves, thickened semicircular protrusions (5) are arranged at symmetrical positions at two ends of the glass slide, a toothed clamp (13) consists of a clamping arm (14), a spring (15), a middle shaft (16) and a toothed protrusion (17), the improved six-hole plate (9) is a hollow cuboid, a partition plate (11) is arranged in the middle of the glass slide to separate two side areas, the plate and the partition plate are provided with a horizontal baffle (10), and 12 fan-shaped glass slide clamping grooves (12) are arranged in the horizontal baffle.
2. The slide combination for animal cell experiments according to claim 1, wherein: the diameter of the slide (1) is 30mm.
3. The slide combination for animal cell experiments according to claim 1, wherein: the thickness of the slide (1) is 3mm.
4. The slide combination for animal cell experiments according to claim 1, wherein: the depths of the first groove (2), the second groove (3) and the third groove (4) in the shape of a truncated cone are half of the thickness of a glass slide, and are 1.5mm.
5. The slide combination for animal cell experiments according to claim 1, wherein: the first groove (2), the second groove (3) and the third groove (4) are of three specifications, and the diameters of the bottoms are 5mm,10mm and 15mm respectively.
6. The slide combination for animal cell experiments according to claim 1, wherein: cell delamination prevention is arranged at the bottoms of the first groove (2), the second groove (3) and the third groove (4) in the shape of a truncated cone.
7. The slide combination for animal cell experiments according to claim 1, wherein: the included angle between the inclined side walls (8) of the first groove (2), the second groove (3) and the third groove (4) and the bottom surface is 60 degrees.
8. The slide combination for animal cell experiments according to claim 1, wherein: the "+" symbol (6) and the "P" symbol (7) change positions correspondingly according to the change of the aperture of the groove.
9. The slide combination for animal cell experiments according to claim 1, wherein: the thickness of the slide clamping groove (12) is consistent with that of the slide (1), and is 3mm.
10. The slide combination for animal cell experiments according to claim 1, wherein: the tooth-shaped clamp (13) is made of plastic material, and the tooth-shaped protrusions (17) are made of soft rubber material.
11. The slide combination for animal cell experiments according to claim 1, wherein: the tail end of the clamping arm (14) of the toothed clamp (13) is provided with anti-skid sand-polishing grains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811198759.XA CN109294915B (en) | 2018-10-15 | 2018-10-15 | Slide combination for animal cell experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811198759.XA CN109294915B (en) | 2018-10-15 | 2018-10-15 | Slide combination for animal cell experiment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109294915A CN109294915A (en) | 2019-02-01 |
| CN109294915B true CN109294915B (en) | 2024-01-02 |
Family
ID=65162582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811198759.XA Active CN109294915B (en) | 2018-10-15 | 2018-10-15 | Slide combination for animal cell experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109294915B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115718024A (en) * | 2022-11-25 | 2023-02-28 | 亚能生物技术（深圳）有限公司 | Immunohistochemical staining method and device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0751215A2 (en) * | 1995-06-29 | 1997-01-02 | Becton, Dickinson and Company | Culture vessel for use with coverslips |
| CN2758756Y (en) * | 2004-09-30 | 2006-02-15 | 中国海洋大学 | Observing bearing device of electrode for electrochemical corrosion experiment |
| CN101665767A (en) * | 2006-08-31 | 2010-03-10 | 李宏 | High-density array type cell growth cover glass experimental device |
-
2018
- 2018-10-15 CN CN201811198759.XA patent/CN109294915B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0751215A2 (en) * | 1995-06-29 | 1997-01-02 | Becton, Dickinson and Company | Culture vessel for use with coverslips |
| CN2758756Y (en) * | 2004-09-30 | 2006-02-15 | 中国海洋大学 | Observing bearing device of electrode for electrochemical corrosion experiment |
| CN101665767A (en) * | 2006-08-31 | 2010-03-10 | 李宏 | High-density array type cell growth cover glass experimental device |
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| Publication number | Publication date |
|---|---|
| CN109294915A (en) | 2019-02-01 |
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| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Hao Jiaqi Inventor after: Cui Jiabin Inventor after: He Qiong Inventor before: Cui Jiabin Inventor before: Hao Jiaqi Inventor before: He Qiong |
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| GR01 | Patent grant |