CN107980612A - A kind of blue quick breeding of short distance groove and tongue and bloom controlling method - Google Patents
A kind of blue quick breeding of short distance groove and tongue and bloom controlling method Download PDFInfo
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- CN107980612A CN107980612A CN201610945799.0A CN201610945799A CN107980612A CN 107980612 A CN107980612 A CN 107980612A CN 201610945799 A CN201610945799 A CN 201610945799A CN 107980612 A CN107980612 A CN 107980612A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 229930006000 Sucrose Natural products 0.000 claims abstract description 12
- 239000008272 agar Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- 239000005720 sucrose Substances 0.000 claims abstract description 12
- 230000010152 pollination Effects 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 239000012869 germination medium Substances 0.000 claims abstract description 7
- 238000002054 transplantation Methods 0.000 claims abstract description 7
- 238000010899 nucleation Methods 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims description 6
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 3
- 241000676840 Elater Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 235000010204 pine bark Nutrition 0.000 claims description 3
- 239000012883 rooting culture medium Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000005728 strengthening Methods 0.000 claims description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000000050 nutritive effect Effects 0.000 claims description 2
- 241000357202 Dendrobium linguiforme Species 0.000 abstract description 10
- 230000004069 differentiation Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000035784 germination Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 4
- 241000233855 Orchidaceae Species 0.000 description 2
- 241000732800 Cymbidium Species 0.000 description 1
- 241000311565 Holcoglossum Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of blue quick breeding of short distance groove and tongue to be with bloom controlling method, its method：（1）Artificial pollination method；（2）Carry out aseptic seeding and carry out sprouting culture, germination medium is 1 NAA+0.05 mg L of 1/2MS+0.1 mg L, 1 6+7 g L of BA+20 g 1 sucrose of L, 1 agar powders；（3）Protocorm Multiplication culture：Its culture medium prescription is 1 NAA+0.2 mg L of MS+0.2 mg L, 1 6+7.5 g L of BA+30 g 1 sucrose of L, 1 agar powders, is cultivated 45 days；（4）Seedling culture：Used root media is 1 sucrose+7.5g L of MS+0.6 mg L 1 NAA+0.2mg L, 16 BA+30 g L, 1 agar powders, and culture can obtain test tube seedling after 60 days；（5）Test tube transplantation of seedlings.Using artificial pollination method of the present invention, setting percentage is up to 85.32%, and fruit is big and full；The rational culture medium of composition proportion is used in each stage that short distance groove and tongue orchid quickly breeds, with germination rate is high, growth coefficient is big, test tube robust seedling, transplanting survival rate are high, the high advantage of bud differentiation rate, the large-scale industrialized production available for short distance groove and tongue orchid.
Description
Technical field
The present invention relates to a kind of blue quick breeding of short distance groove and tongue with bloom controlling method, the Cultivating techniques for belonging to tissue-cultured seedling are led
Domain.
Background technology
Short distance groove and tongue orchid is orchid family, groove and tongue Cymbidium（Holcoglossum Schltr）Miniature single-shaft class plant, grow nonparasitically upon another plant more in
On forest trunk or on hayashishita stone.Short distance groove and tongue orchid is Chinese unique wheat, belongs to pocket orchid, its form is graceful, leaf delicate and pretty, flower
Color is gorgeous, with simple and elegant delicate fragrance, can plant and be viewed and admired in shallow basin or suspension, very elegant.In the wild due to living environment forfeiture, growth
Slowly, monopodial branching, the artificial excessively reason groove and tongue orchid such as excavation have belonged to Endangered taxa in China.At present, both at home and abroad in relation to groove and tongue
Blue research and report is seldom, only reports for work its systematics, pollination system change, Endangered status, has not seen related groove and tongue
The report of blue plant conservation biological study and artificial propagation.According to short distance groove and tongue orchid artificial domesticating cultivation and related with reproduction
Biological characteristics, high efficiency quick breeding system is established by seed bottle broadcasting method.There is provided a kind of blue quick breeding of short distance groove and tongue with into
Flower regulation and control method will be of great significance, and then protect wild resource in imminent danger.
The content of the invention
The technical problem to be solved in the present invention is：A kind of blue quick breeding of short distance groove and tongue and bloom controlling method are provided, with
Solve the problems, such as that short distance groove and tongue orchid percentage of seedgermination is low, seedling rooting rate is low, slow-growing, accelerate the life of short distance groove and tongue orchid species seedling
It is long.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of blue quick breeding of short distance groove and tongue and bloom controlling method, it is characterised in that comprise the following steps：
（1）Artificial pollination method：Foliage application two is carried out using 0.2% ammonium nitrate when flower arrow occurs in the blue growth of short distance groove and tongue
It is secondary, its interval two weeks, then bud formed early period with 0.15% boric acid foliage application once, Post flowering artificial pollination using together
Kind of different strain pollinating method, retains two or more massula on every style, at most retains two fruits on last each flower arrow；
（2）Carry out aseptic seeding：The fruit of 150 days after pollinating is taken, is soaked with behind 75% alcohol wipe surface with 0.15% mercury chloride
15 minutes, it is stirred therebetween with magnetic stirring apparatus, fruit is cut on sterile filter paper after aseptic water washing 2 times, by fruit pod
Interior seed and elater is seeded on germination medium together, and germination medium is 1/2MS+0.1 mg L-1 NAA+0.05
+ 7 g L-1 agar powders of mg L-1 6-BA+20 g L-1 sucrose；
（3）Protocorm Multiplication culture：It is transferred to green protocorm is turned after sprouting completely on Protocorm Multiplication culture medium, it is cultivated
Based formulas is+7.5 g L-1 agar powders of MS+0.2 mg L-1 NAA+0.2 mg L-1 6-BA+30 g L-1 sucrose, culture 45
My god；
（4）Seedling culture：The protocorm of propagation is transferred on strengthening seedling and rooting culture medium and is cultivated, root media MS+0.6
Mg L-1 NAA+0.2mg L-1 6-BA+30 g L-1 sucrose+7.5g L-1 agar powders, culture can obtain test tube seedling after 60 days；
（5）Test tube transplantation of seedlings.
Preferably, step（5）Test tube transplantation of seedlings concretely comprises the following steps：Choose 5-8 centimetres of height, there is the excellent of 6-7 piece leaves
Matter test tube seedling, hardening one week, taking-up seedling gently wash away root portion culture medium, each 1 cun of transparent nutrition in greenhouse after uncapping
1 seedling is cultivated in alms bowl, cultivation matrix is 0.3-0.6 centimetres of pine bark, and 30 days 180 days special after being affixed by More section in cultivation matrix
Slow released fertilizer is per 3 grams of basin, and monthly 1000 times of flowers of foliage-spray are the more 1 time fertile.
Compared with prior art, beneficial effects of the present invention are as follows：Using artificial pollination method of the present invention, setting percentage is up to
85.32%, fruit is big and full；The rational culture medium of composition proportion is used in each stage that short distance groove and tongue orchid quickly breeds,
With germination rate is high, growth coefficient is big, test tube robust seedling, transplanting survival rate are high, the high advantage of bud differentiation rate, seedling transplant survival
Rate is up to more than 95%, and bud differentiation rate is up to more than 85% then, the large-scale industrialized production available for short distance groove and tongue orchid.
Embodiment
Invention is described further with reference to specific embodiment：
A kind of blue quick breeding of short distance groove and tongue and bloom controlling method, it is characterised in that comprise the following steps：
（1）Artificial pollination method：Foliage application two is carried out using 0.2% ammonium nitrate when flower arrow occurs in the blue growth of short distance groove and tongue
It is secondary, its interval two weeks, then bud formed early period with 0.15% boric acid foliage application once, Post flowering artificial pollination using together
Kind of different strain pollinating method, retains two or more massula on every style, at most retains two fruits on last each flower arrow；
（2）Carry out aseptic seeding：The fruit of 150 days after pollinating is taken, is soaked with behind 75% alcohol wipe surface with 0.15% mercury chloride
15 minutes, it is stirred therebetween with magnetic stirring apparatus, fruit is cut on sterile filter paper after aseptic water washing 2 times, by fruit pod
Interior seed and elater is seeded on germination medium together, and germination medium is 1/2MS+0.1 mg L-1 NAA+0.05
+ 7 g L-1 agar powders of mg L-1 6-BA+20 g L-1 sucrose；
（3）Protocorm Multiplication culture：It is transferred to green protocorm is turned after sprouting completely on Protocorm Multiplication culture medium, it is cultivated
Based formulas is+7.5 g L-1 agar powders of MS+0.2 mg L-1 NAA+0.2 mg L-1 6-BA+30 g L-1 sucrose, culture 45
My god；
（4）Seedling culture：The protocorm of propagation is transferred on strengthening seedling and rooting culture medium and is cultivated, root media MS+0.6
Mg L-1 NAA+0.2mg L-1 6-BA+30 g L-1 sucrose+7.5g L-1 agar powders, culture can obtain test tube seedling after 60 days；
（5）Test tube transplantation of seedlings.
In the present embodiment, step（5）Test tube transplantation of seedlings concretely comprises the following steps：Choose 5-8 centimetres of height, there is 6-7 pieces
The high-quality test tube seedling of leaf, hardening one week, taking-up seedling gently wash away root portion culture medium in greenhouse after uncapping, and each 1 cun thoroughly
1 seedling is cultivated in bright nutritive cube, cultivation matrix is 0.3-0.6 centimetres of pine bark, and 30 days special after being affixed by More section in cultivation matrix
180 days slow released fertilizers are per 3 grams of basin, and monthly 1000 times of flowers of foliage-spray are the more 1 time fertile.
Above content is that a further detailed description of the present invention in conjunction with specific preferred embodiments, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (2)
1. a kind of blue quick breeding of short distance groove and tongue and bloom controlling method, it is characterised in that comprise the following steps：
（1）Artificial pollination method：Foliage application two is carried out using 0.2% ammonium nitrate when flower arrow occurs in the blue growth of short distance groove and tongue
It is secondary, its interval two weeks, then bud formed early period with 0.15% boric acid foliage application once, Post flowering artificial pollination using together
Kind of different strain pollinating method, retains two or more massula on every style, at most retains two fruits on last each flower arrow；
（2）Carry out aseptic seeding：The fruit of 150 days after pollinating is taken, is soaked with behind 75% alcohol wipe surface with 0.15% mercury chloride
15 minutes, it is stirred therebetween with magnetic stirring apparatus, fruit is cut on sterile filter paper after aseptic water washing 2 times, by fruit pod
Interior seed and elater is seeded on germination medium together, and germination medium is 1/2MS+0.1 mg L-1 NAA+0.05
+ 7 g L-1 agar powders of mg L-1 6-BA+20 g L-1 sucrose；
（3）Protocorm Multiplication culture：It is transferred to green protocorm is turned after sprouting completely on Protocorm Multiplication culture medium, it is cultivated
Based formulas is+7.5 g L-1 agar powders of MS+0.2 mg L-1 NAA+0.2 mg L-1 6-BA+30 g L-1 sucrose, culture 45
My god；
（4）Seedling culture：The protocorm of propagation is transferred on strengthening seedling and rooting culture medium and is cultivated, root media MS+0.6
Mg L-1 NAA+0.2mg L-1 6-BA+30 g L-1 sucrose+7.5g L-1 agar powders, culture can obtain test tube seedling after 60 days；
（5）Test tube transplantation of seedlings.
2. the blue quick breeding of a kind of short distance groove and tongue according to claim 1 and bloom controlling method, it is characterised in that：Step
（5）Test tube transplantation of seedlings concretely comprises the following steps：Choose 5-8 centimetres of height, there is the high-quality test tube seedling of 6-7 piece leaves, big after uncapping
Hardening one week in canopy, takes out seedling and gently washes away root portion culture medium, 1 seedling, cultivation matrix are cultivated in each 1 cun of transparent nutritive cube
For 0.3-0.6 centimetres of pine bark, 30 days after being affixed by special 180 days slow released fertilizers of More section in cultivation matrix per 3 grams of basin, and monthly
1000 times of flowers of foliage-spray are the more 1 time fertile.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945799.0A CN107980612A (en) | 2016-10-26 | 2016-10-26 | A kind of blue quick breeding of short distance groove and tongue and bloom controlling method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610945799.0A CN107980612A (en) | 2016-10-26 | 2016-10-26 | A kind of blue quick breeding of short distance groove and tongue and bloom controlling method |
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| Publication Number | Publication Date |
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| CN107980612A true CN107980612A (en) | 2018-05-04 |
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| CN201610945799.0A Pending CN107980612A (en) | 2016-10-26 | 2016-10-26 | A kind of blue quick breeding of short distance groove and tongue and bloom controlling method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109964817A (en) * | 2019-04-16 | 2019-07-05 | 四川省自然资源科学研究院 | The rapid propagation method of high eyebrow groove and tongue orchid |
| CN118104567A (en) * | 2024-04-29 | 2024-05-31 | 云南省林业和草原科学院 | A method for long-term preservation of living regeneration and efficient propagation of short-spurred groove tongue orchid |
-
2016
- 2016-10-26 CN CN201610945799.0A patent/CN107980612A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109964817A (en) * | 2019-04-16 | 2019-07-05 | 四川省自然资源科学研究院 | The rapid propagation method of high eyebrow groove and tongue orchid |
| CN109964817B (en) * | 2019-04-16 | 2022-03-11 | 四川省自然资源科学研究院 | Rapid propagation method of Emei glauca |
| CN118104567A (en) * | 2024-04-29 | 2024-05-31 | 云南省林业和草原科学院 | A method for long-term preservation of living regeneration and efficient propagation of short-spurred groove tongue orchid |
| CN118104567B (en) * | 2024-04-29 | 2024-06-28 | 云南省林业和草原科学院 | A method for long-term preservation of living regeneration and efficient propagation of short-spurred groove tongue orchid |
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Application publication date: 20180504 |
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| WD01 | Invention patent application deemed withdrawn after publication |