CN106615117A - Stirring type fruit-flavor goat milk kefir and preparation method thereof - Google Patents
Stirring type fruit-flavor goat milk kefir and preparation method thereof Download PDFInfo
- Publication number
- CN106615117A CN106615117A CN201710018973.1A CN201710018973A CN106615117A CN 106615117 A CN106615117 A CN 106615117A CN 201710018973 A CN201710018973 A CN 201710018973A CN 106615117 A CN106615117 A CN 106615117A
- Authority
- CN
- China
- Prior art keywords
- milk
- kefir
- goat
- juice
- fruit juice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000015141 kefir Nutrition 0.000 title claims description 75
- 235000020251 goat milk Nutrition 0.000 title claims description 54
- 238000003756 stirring Methods 0.000 title claims description 34
- 238000002360 preparation method Methods 0.000 title claims description 16
- 239000008369 fruit flavor Substances 0.000 title claims description 7
- 102000007544 Whey Proteins Human genes 0.000 claims description 57
- 108010046377 Whey Proteins Proteins 0.000 claims description 57
- 235000015203 fruit juice Nutrition 0.000 claims description 52
- 235000021119 whey protein Nutrition 0.000 claims description 52
- 235000020185 raw untreated milk Nutrition 0.000 claims description 37
- 239000007788 liquid Substances 0.000 claims description 32
- 239000001814 pectin Substances 0.000 claims description 22
- 235000010987 pectin Nutrition 0.000 claims description 22
- 229920001277 pectin Polymers 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 19
- 239000007858 starting material Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 235000018102 proteins Nutrition 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 11
- 240000000851 Vaccinium corymbosum Species 0.000 claims description 9
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 9
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 9
- 235000021014 blueberries Nutrition 0.000 claims description 9
- 235000013944 peach juice Nutrition 0.000 claims description 9
- 235000013948 strawberry juice Nutrition 0.000 claims description 9
- 235000000346 sugar Nutrition 0.000 claims description 9
- 239000005862 Whey Substances 0.000 claims description 5
- 239000000839 emulsion Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 description 32
- 239000012460 protein solution Substances 0.000 description 11
- 239000000796 flavoring agent Substances 0.000 description 9
- 235000019634 flavors Nutrition 0.000 description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 235000019640 taste Nutrition 0.000 description 6
- 240000001046 Lactobacillus acidophilus Species 0.000 description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 5
- 239000005018 casein Substances 0.000 description 5
- 235000021001 fermented dairy product Nutrition 0.000 description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 5
- 230000001953 sensory effect Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 235000014048 cultured milk product Nutrition 0.000 description 4
- 235000021552 granulated sugar Nutrition 0.000 description 4
- 239000005457 ice water Substances 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002562 thickening agent Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 244000057717 Streptococcus lactis Species 0.000 description 3
- 235000014897 Streptococcus lactis Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- -1 C 6 Chemical class 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 description 2
- 102000008192 Lactoglobulins Human genes 0.000 description 2
- 108010060630 Lactoglobulins Proteins 0.000 description 2
- 241000192132 Leuconostoc Species 0.000 description 2
- 241001468195 Leuconostoc mesenteroides subsp. mesenteroides Species 0.000 description 2
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 description 2
- 241000194020 Streptococcus thermophilus Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000019264 food flavour enhancer Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 235000021391 short chain fatty acids Nutrition 0.000 description 2
- 150000004666 short chain fatty acids Chemical class 0.000 description 2
- 235000019614 sour taste Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000010855 food raising agent Nutrition 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 102000034238 globular proteins Human genes 0.000 description 1
- 108091005896 globular proteins Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000015094 jam Nutrition 0.000 description 1
- 235000021581 juice product Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/137—Thickening substances
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dairy Products (AREA)
Abstract
本发明的果味搅拌型羊奶开菲尔属于乳制品领域。果味搅拌型羊奶开菲尔的主要成分有原料乳、糖、果汁、聚合乳清蛋白、果胶、开菲尔发酵剂。果汁为浓缩果汁经复原制得。聚合乳清蛋白溶液采用中性热聚合的方法制得;采用两次发酵和添加浓缩果汁的办法,使酸凝胶形成的三维网状结构,发挥增稠、增强果汁稳定性和羊奶膻味掩盖作用,解决了现有技术添加果汁造成发酵乳制品质量下降的难题。开菲尔发酵剂为乳酸乳球菌、明串珠菌、嗜热链球菌、嗜酸乳杆菌等组成的商业化直投式发酵剂。本发明所制得的果味搅拌型羊奶开菲尔具有高益生菌含量、高蛋白质含量、高粘度水平、低脱水收缩率、低羊奶膻味,风味独特,色泽均匀,质地细腻等特点。The fruity stirred goat's milk kefir of the invention belongs to the field of dairy products. The main ingredients of fruity stirred goat milk kefir are raw milk, sugar, fruit juice, polywhey protein, pectin, and kefir starter. Fruit juice is obtained by reconcentrating concentrated fruit juice. The polymerized whey protein solution is prepared by neutral heat polymerization; two fermentations and the addition of concentrated fruit juice are used to make the three-dimensional network structure formed by the acid gel play a role in thickening, enhancing the stability of the juice and the smell of goat milk The masking function solves the difficult problem of the decline in the quality of fermented milk products caused by the addition of fruit juice in the prior art. Kefir starter is a commercial direct-injection starter composed of Lactococcus lactis, Leuconostoc, Streptococcus thermophilus, and Lactobacillus acidophilus. The fruit-flavored goat's milk kefir prepared by the present invention has the characteristics of high probiotic content, high protein content, high viscosity level, low syneresis, low goat's milk smell, unique flavor, uniform color and fine texture, etc. .
Description
技术领域technical field
本发明属于乳制品的技术领域,特别是涉及一种果味搅拌型羊奶开菲尔及其制备方法。The invention belongs to the technical field of dairy products, and in particular relates to a fruity stirred type goat milk kefir and a preparation method thereof.
背景技术Background technique
开菲尔作为一种古老的发酵乳制品起源于高加索地区,距今已经有1400多年的历史。开菲尔具有多种健康功能,例如降低胆固醇、抗菌、抗癌等。传统的开菲尔由一种名为开菲尔粒(Kefir grains)的发酵剂发酵制得。开菲尔粒是一种由乳酸菌、明串珠菌、酵母菌及醋酸菌组成的微生物共生体系,它们共生在一个由粘性多糖组成的具有不规则外形的半硬质颗粒上。由于微生物的种属不同,导致各自的代谢产物也不同,这就赋予了开菲尔独特的风味特征。但是,由于传统开菲尔发酵剂中次生酵母的二次发酵作用,使得产品在储藏过程中会因酵母产气而导致包装漏气,这直接影响了产品的质量。为了解决这一个问题,商业化的直投式开菲尔发酵剂被应用于开菲尔的生产加工过程。它由传统开菲尔粒中分离得到的一些菌种组成,在保留传统开菲尔独特风味的同时,也妥善解决了酵母二次发酵导致的包装漏气的问题。Kefir, as an ancient fermented dairy product, originated in the Caucasus region with a history of more than 1,400 years. Kefir has many health functions, such as lowering cholesterol, antibacterial, anticancer and so on. Traditional kefir is made by fermenting a leavening agent called kefir grains. Kefir grains are a microbial symbiosis system composed of lactic acid bacteria, leuconostoc, yeast and acetic acid bacteria, which symbiosis on a semi-hard granule with an irregular shape composed of viscous polysaccharides. Due to the different species of microorganisms, their metabolites are also different, which endows kefir with unique flavor characteristics. However, due to the secondary fermentation of the secondary yeast in the traditional kefir starter, the product will leak in the packaging due to the gas produced by the yeast during storage, which directly affects the quality of the product. In order to solve this problem, a commercial direct-throwing kefir starter is applied to the production and processing of kefir. It is composed of some strains isolated from traditional kefir grains. While retaining the unique flavor of traditional kefir, it also properly solves the problem of packaging leakage caused by the secondary fermentation of yeast.
羊奶作为世界第三大乳源,在发展中国家的营养膳食中具有重要的地位。与牛奶相比,羊奶中较多的短链脂肪酸和较少的酪蛋白含量使得它更容易被人体吸收且具有较低的致敏性。但是较多的具有挥发性的短链脂肪酸(如C6、C8、C10)的存在使羊奶制品具有独特的膻味,较少的酪蛋白含量使发酵羊奶制品具有低粘度、低硬度、高脱水收缩率的特性,这些缺陷严重制约了发酵羊奶产业的发展。As the third largest milk source in the world, goat milk plays an important role in the nutritional diet of developing countries. Compared with cow's milk, goat's milk has more short-chain fatty acids and less casein content, making it more easily absorbed by the body and less allergenic. However, the presence of more volatile short-chain fatty acids (such as C 6 , C 8 , and C 10 ) makes goat milk products have a unique smell, and less casein content makes fermented goat milk products have low viscosity and low viscosity. Hardness, high syneresis, these defects seriously restrict the development of fermented goat milk industry.
乳清蛋白是由干酪或干酪素生产过程中所产生的副产品乳清经过特殊工艺浓缩精制而得的一类蛋白质。根据其蛋白含量的高低,乳清蛋白产品又分为乳清蛋白分离体(WPI)和浓缩乳清蛋白(WPC)。乳清蛋白中含量最高的蛋白为β-乳球蛋白,约占乳清蛋白总量的50%,是一种由162个氨基酸折叠成的紧密球状蛋白质。在中性条件(pH≈7)下对乳清蛋白进行加热改性能够使β-乳球蛋白分子多肽链的三级结构被破坏,去折叠,暴露疏水基团,最终蛋白质分子聚集成稳定的聚合乳清蛋白溶液但不形成凝胶。在开菲尔发酵过程中,pH会因微生物发酵产物的影响而逐渐降低,使加入其中的聚合乳清蛋白溶液逐渐接近等电点,此时分子间的斥力势能不足以维持体系的稳定,蛋白分子发生聚集并形成三维网状结构。由此形成的蛋白网络可以通过毛细管力来持蓄水分,并会包埋住部分物质。因此可以用于增强开菲尔凝乳的硬度、粘度、持水力,并且可以起到部分掩盖羊奶膻味的作用。Whey protein is a type of protein obtained by concentrating and refining the by-product whey produced in the process of cheese or casein production through a special process. According to its protein content, whey protein products are divided into whey protein isolate (WPI) and whey protein concentrate (WPC). The protein with the highest content in whey protein is β-lactoglobulin, accounting for about 50% of the total whey protein. It is a compact globular protein folded by 162 amino acids. Heat modification of whey protein under neutral conditions (pH ≈ 7) can destroy the tertiary structure of the polypeptide chain of β-lactoglobulin molecules, unfold, expose hydrophobic groups, and finally protein molecules aggregate into stable The whey protein solution polymerized but did not form a gel. During the kefir fermentation process, the pH will gradually decrease due to the influence of microbial fermentation products, so that the polymerized whey protein solution added to it will gradually approach the isoelectric point. At this time, the repulsion potential energy between molecules is not enough to maintain the stability of the system. The molecules aggregate and form a three-dimensional network. The resulting protein network can hold water through capillary forces and entrap some substances. Therefore, it can be used to enhance the hardness, viscosity and water holding capacity of kefir curd milk, and can partially cover up the smell of goat milk.
果汁是指将水果经过压榨、离心、萃取得到的汁液产品。果汁中保留有水果中较大部分的营养成分,例如各种维生素、矿物质、糖分和膳食纤维等。饮用果汁可以促进消化、润滑肠道、补充营养。由于新鲜果汁不易保存且运输成本较高,因此通常采用低温真空浓缩的方法将新鲜果汁制备得到浓缩果汁,后期再通过添加等量纯净水的方法将其复原。果汁可以应用于多种食品领域,通过添加果汁改善产品风味已经越来越被市场所青睐。Juice refers to the juice product obtained by pressing, centrifuging and extracting fruit. The fruit juice retains most of the nutrients in the fruit, such as various vitamins, minerals, sugar and dietary fiber. Drinking fruit juice can promote digestion, lubricate the intestines, and supplement nutrition. Since fresh juice is not easy to preserve and has high transportation costs, it is usually prepared by low-temperature vacuum concentration to obtain concentrated juice, which is then recovered by adding an equal amount of purified water later. Juice can be used in a variety of food fields, and the addition of juice to improve product flavor has become more and more popular in the market.
目前果味搅拌型发酵乳制品中果料的加入通常选择纯果汁、浓缩果汁、果酱、果粉等,但它们都存在一定的缺陷。由于纯果汁中水分含量过大,因此它的加入会降低产品固形物含量且果汁上浮现象明显。浓缩果汁的加入虽然可以解决由纯果汁的问题,但是由于浓缩果汁pH普遍偏低,因此它的加入可能会造成产品pH急剧下降,从而导致乳清析出现象发生,影响产品感官性状。At present, pure fruit juice, concentrated fruit juice, jam, fruit powder, etc. are usually selected as the addition of fruit material in fruity stirred fermented dairy products, but they all have certain defects. Since the water content in the pure fruit juice is too large, its addition will reduce the solid content of the product and the fruit juice will obviously float. Although the addition of concentrated fruit juice can solve the problem of pure fruit juice, but because the pH of concentrated fruit juice is generally low, its addition may cause a sharp drop in product pH, resulting in whey precipitation and affecting the sensory properties of the product.
发明内容Contents of the invention
本发明所要解决的技术问题是提供一种果味搅拌型羊奶开菲尔的制备方法。通过加入浓缩果汁赋予搅拌型羊奶开菲尔多种多样的风味。加入聚合乳清蛋白不仅起到了增稠、增加果汁稳定性的作用,同时也与果汁相互配合,共同掩盖了羊奶的膻味。开菲尔发酵剂中的多种益生菌能够增强产品的保健功能。最终制得一款风味独特、羊奶膻味轻、营养价值高、稳定性好的发酵乳制品。The technical problem to be solved by the present invention is to provide a method for preparing fruity stirred goat milk kefir. Blended goat's milk kefir has a variety of flavors by adding concentrated fruit juice. The addition of polymerized whey protein not only plays a role in thickening and increasing the stability of the juice, but also cooperates with the juice to cover up the smell of goat milk. A variety of probiotics in kefir starter can enhance the health function of the product. Finally, a fermented dairy product with unique flavor, light goat milk smell, high nutritional value and good stability was obtained.
本发明中另两个要解决的技术问题是聚合乳清蛋白的制备方法与果汁的加入方式。聚合乳清蛋白制备方法较为简单,相关技术较为成熟,但本发明作为搅拌型开菲尔的主要增稠剂、果汁稳定剂和膻味掩盖剂尚属首次。由于果汁中水分含量较高,若直接添加纯果汁对于产品固形物含量影响较大,因此本发明采用浓缩果汁以羊奶开菲尔基料替代水复原的方式制备果味搅拌型羊奶开菲尔。The other two technical problems to be solved in the present invention are the preparation method of polymerized whey protein and the way of adding fruit juice. The preparation method of polymerized whey protein is relatively simple, and the relevant technology is relatively mature, but it is the first time that the present invention is used as the main thickener, fruit juice stabilizer and smell masking agent of stirred kefir. Since the water content in fruit juice is relatively high, adding pure fruit juice directly will have a greater impact on the solid content of the product. Therefore, the present invention uses concentrated fruit juice and uses goat milk kefir base material instead of water to reconstitute to prepare fruity stirred goat milk kefir. you.
本发明解决问题的技术方案是:The technical scheme that the present invention solves the problem is:
一种果味搅拌型羊奶开菲尔,其制备原料包括下列成份及其用量:液态原料乳、果汁占液态原料乳体积的5%~20%、糖占液态原料乳重量的5%~8%、聚合乳清蛋白占液态原料乳重量的0.1%~0.6%、果胶占液态原料乳重量的0.1%~0.3%、开菲尔发酵剂占液态原料乳重量的0.005%~0.03%;其中,果汁按体积浓缩至比例为1:5~10使用。A fruit-flavored goat's milk kefir. The raw materials for its preparation include the following ingredients and their dosages: liquid raw milk, fruit juice accounting for 5% to 20% of the volume of the liquid raw milk, and sugar accounting for 5% to 8% of the weight of the liquid raw milk. %, polymerized whey protein accounts for 0.1% to 0.6% of the weight of liquid raw milk, pectin accounts for 0.1% to 0.3% of the weight of liquid raw milk, and kefir starter accounts for 0.005% to 0.03% of the weight of liquid raw milk; , the juice is concentrated by volume to a ratio of 1:5-10 for use.
所述的果汁,是黄桃汁和/或草莓汁和/或蓝莓汁。Described fruit juice is yellow peach juice and/or strawberry juice and/or blueberry juice.
所述开菲尔发酵剂是由乳酸乳球菌乳酸亚种(Lactococcus lactissubsp.lactis)、乳酸乳球菌乳酸亚种丁二酮变种(Lactococcus lactissubsp.Lactisbiovar.diacetylactis)、肠膜明串珠菌肠膜亚种(Leuconostoc mesenteroidessubsp.mesenteroides)、嗜热链球菌(Streptococcus thermonphilus)、嗜酸乳杆菌(Lactobacillus acidophilus)组成。The kefir starter is made of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. Lactis biovar. diacetylactis, Leuconostoc enterococci (Leuconostoc mesenteroides subsp.mesenteroides), Streptococcus thermophilus (Streptococcus thermonphilus), Lactobacillus acidophilus (Lactobacillus acidophilus).
所述果胶为低酯单体果胶,酯化度为30%以下。The pectin is low-ester monomer pectin, and the degree of esterification is below 30%.
本发明的果味搅拌型羊奶开菲尔,以果汁为风味增强剂,以聚合乳清蛋白为增稠剂和果汁稳定剂,以果胶为辅助增稠剂,以聚合乳清蛋白和果汁共为羊奶膻味掩盖剂,其固形物含量在15%以上,蛋白质含量在2.50%~3.50%,脂肪含量在2.60%~2.90%,pH在4.2~4.6之间,稳定性良好,无果汁上浮,无乳清析出,粘度在1189mPa.s以上。The fruit flavor stirring goat milk kefir of the present invention uses fruit juice as a flavor enhancer, polymerized whey protein as a thickener and juice stabilizer, pectin as an auxiliary thickener, polymerized whey protein and fruit juice A total of goat milk odor masking agent, its solid content is more than 15%, protein content is 2.50%-3.50%, fat content is 2.60%-2.90%, pH is between 4.2-4.6, good stability, no fruit juice Floating, no whey precipitation, viscosity above 1189mPa.s.
本发明的果味搅拌型羊奶开菲尔制备方法的技术方案如下。The technical scheme of the preparation method of the fruit flavor stirring type goat milk kefir of the present invention is as follows.
一种果味搅拌型羊奶开菲尔的制备方法,有以下制备步骤:将果汁按体积比例1:5~10浓缩为浓缩果汁;将糖、果胶预先混合,添加到液态原料乳中,搅拌加热至50~55℃,待果胶完全溶解得到混合原料乳;将聚合乳清蛋白加入混合原料乳中,搅拌加热至80~90℃,保温10~20min杀菌,冰水浴冷却至25~35℃,加入开菲尔发酵剂搅拌均匀,25~35℃初发酵8~14h至pH低于4.6,破乳并搅拌均匀,得到羊奶开菲尔基料;在羊奶开菲尔基料中加入浓缩果汁,搅拌均匀后分装,并于4~6℃后发酵12~24h,得到果味搅拌型羊奶开菲尔。A method for preparing fruit-flavored goat's milk kefir, comprising the following preparation steps: concentrating fruit juice into concentrated fruit juice at a volume ratio of 1:5-10; pre-mixing sugar and pectin, adding it to liquid raw milk, Stir and heat to 50-55°C until the pectin is completely dissolved to obtain mixed raw milk; add polywhey protein to the mixed raw milk, stir and heat to 80-90°C, keep it warm for 10-20 minutes to sterilize, cool in an ice-water bath to 25-35 ℃, add kefir starter and stir evenly, ferment for 8-14 hours at 25-35℃ until the pH is lower than 4.6, break the emulsion and stir evenly to obtain goat milk kefir base material; in goat milk kefir base material Add concentrated fruit juice, stir evenly, then pack, and post-ferment at 4-6°C for 12-24 hours to obtain fruity-flavored goat's milk kefir.
所述的聚合乳清蛋白,是乳清蛋白分离体和/或浓缩乳清蛋白在pH=7,温度80~85℃的条件下保温30min,后冷却得到的。具体的可以是:按乳清蛋白分离体或浓缩乳清蛋白中纯蛋白的含量,以纯蛋白与水的质量比1:10的比例溶解于水中,磁力搅拌30~60min致其完全溶解,4~6℃条件下放置10~12h,使其充分水合;使用1~3M的氢氧化钠溶液将乳清蛋白分离体溶液调至pH=7,水浴升温至80~85℃保温30min,使其充分变性,冰浴冷却,并在4~6℃下贮藏待用。制得的聚合乳清蛋白溶液中按质量计含聚合乳清蛋白约为10%~11%。The polymerized whey protein is obtained by keeping whey protein isolate and/or concentrated whey protein at pH=7 and temperature 80-85° C. for 30 minutes, and then cooled. Specifically, it can be: according to the content of pure protein in whey protein isolate or concentrated whey protein, dissolve it in water at a mass ratio of pure protein to water of 1:10, stir magnetically for 30-60 minutes until it is completely dissolved, 4- Place it at 6°C for 10-12 hours to make it fully hydrated; use 1-3M sodium hydroxide solution to adjust the whey protein isolate solution to pH = 7, heat the water bath to 80-85°C and keep it for 30 minutes to fully denature it , cooled in an ice bath, and stored at 4-6°C until use. The prepared polymerized whey protein solution contains about 10% to 11% of the polymerized whey protein by mass.
上述的乳清蛋白分离体,蛋白质含量按质量计为90%~95%,浓缩乳清蛋白中蛋白质含量按质量计为75%~85%。The above-mentioned whey protein isolate has a protein content of 90% to 95% by mass, and the protein content of the concentrated whey protein is 75% to 85% by mass.
本发明的果味搅拌型羊奶开菲尔制备方法中,所述的糖,是白砂糖和/或绵白糖;所述的果胶,是低酯单体果胶,酯化度为30%以下;所述的开菲尔发酵剂,是丹尼斯克(Danisco)公司生产的Kefir Mild 01直投式发酵剂,主要由乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.lactis)、乳酸乳球菌乳酸亚种丁二酮变种(Lactococcuslactissubsp.Lactis biovar.diacetylactis)、肠膜明串珠菌肠膜亚种(Leuconostocmesenteroides subsp.mesenteroides)、嗜热链球菌(Streptococcus thermonphilus)、嗜酸乳杆菌(Lactobacillus acidophilus)组成。In the preparation method of the fruity stirred goat milk kefir of the present invention, the sugar is white granulated sugar and/or soft white sugar; the pectin is low-ester monomeric pectin, and the degree of esterification is 30% Below; Described kefir starter is the Kefir Mild 01 direct-throwing starter produced by Danisco (Danisco), mainly composed of Lactococcus lactis subsp.lactis, Lactococcus lactis subsp. Lactococcus lactis subsp. Lactis biovar. diacetylactis, Leuconostocmesenteroides subsp. mesenteroides, Streptococcus thermophilus, Lactobacillus acidophilus.
本发明的果味搅拌型羊奶开菲尔制备方法中,果味搅拌型羊奶开菲尔中所述的果汁,是以羊奶开菲尔基料替代纯净水复原浓缩果汁后得到的。In the preparation method of the fruit-flavored stirred goat's milk kefir of the present invention, the fruit juice described in the fruity-flavored stirred goat's milk kefir is obtained by reconstituting the concentrated fruit juice with the goat's milk kefir base material instead of pure water.
本发明的食用方法及食用量同食用通常的搅拌型发酵乳制品。The eating method and the consumption amount of the present invention are the same as those of eating common stirred type fermented milk products.
本发明的方法中,“初发酵”过程是利用聚合乳清蛋白在pH降低过程中与果胶、酪蛋白发生交联形成三维网状结构发挥了增稠作用。但是在浓缩果汁加入到初发酵后的开菲尔基料中,会造成开菲尔基料pH产生一定程度的下降,这时初发酵未交联的聚合乳清蛋白由于pH的下降再次产生凝胶化反应,将果汁中的大分子物质包埋,同时与前面形成的三维网状结构互相链接,形成更加稳定的复合结构。因此在添加浓缩果汁的时候,产品不会因为pH的下降而质量下降,反而会更加稳定和均匀。这极大增强了最终产品的均一性和稳定性,避免了果味发酵乳制品中果料上浮或者沉淀的问题。这种对于聚合乳清蛋白酸凝胶应用的新思路,解决了以浓缩果汁作为主要果料制备果味搅拌型发酵乳制品的一个难题。In the method of the present invention, the "initial fermentation" process utilizes the polymerized whey protein to cross-link with pectin and casein to form a three-dimensional network structure during the pH lowering process to play a thickening effect. However, when the concentrated fruit juice is added to the kefir base material after initial fermentation, the pH of the kefir base material will drop to a certain extent, and at this time, the uncrosslinked polymerized whey protein of the initial fermentation will produce condensation again due to the drop in pH. The gelation reaction embeds the macromolecular substances in the fruit juice, and at the same time links with the three-dimensional network structure formed earlier to form a more stable composite structure. Therefore, when adding concentrated fruit juice, the quality of the product will not decrease due to the drop in pH, but will be more stable and uniform. This greatly enhances the uniformity and stability of the final product, and avoids the problem of floating or settling fruit in fruity fermented dairy products. This new idea for the application of polymerized whey protein acid gel solves a difficult problem in the preparation of fruit-flavored stirred fermented milk products with concentrated fruit juice as the main fruit material.
同时,聚合乳清蛋白-果汁料分子-果胶-酪蛋白形成的复合结构可以将羊奶中短链的挥发性脂肪酸(如C6、C8、C10)和开菲尔发酵剂产生的部分酸味分子包埋其中,由于此结构已经达到了分子级别,所以在口腔咀嚼过程中不会被破坏,这也就降低了产品的膻味和开菲尔特殊的酸味,增强了产品的口感和气味。解决了羊奶开菲尔特殊的酸味和膻味问题。At the same time, the composite structure formed by polymerized whey protein-juice material molecule-pectin-casein can combine the short-chain volatile fatty acids (such as C 6 , C 8 , C 10 ) in goat milk and those produced by kefir starter Part of the sour taste molecules are embedded in it. Since this structure has reached the molecular level, it will not be destroyed during oral chewing, which also reduces the product's smell and the special sour taste of kefir, and enhances the taste and taste of the product. odor. It solves the special sour and muttony problems of goat milk kefir.
本发明产品果味搅拌型羊奶开菲尔作为一种以果汁作为风味增强剂,以聚合乳清蛋白为主要增稠剂和果汁稳定剂,以果汁和聚合乳清蛋白为羊奶膻味掩盖剂,并含开菲尔益生菌群的新型发酵乳制品,该产品原料羊奶具有营养价值高、营养成分易吸收、低致敏性的特点,所用开菲尔发酵剂制得的开菲尔具有降低胆固醇、抗菌、抗癌的特点。本发明产品蛋白质含量高、甜度适中、羊奶膻味低、风味独特,此外还具有质地细腻、结构均一、色泽优美、口感较好的特点。The product of the present invention is a fruity stirred type goat milk kefir, which uses fruit juice as a flavor enhancer, polymerized whey protein as a main thickener and juice stabilizer, and fruit juice and polymerized whey protein as a goat milk smell mask It is a new type of fermented milk product containing kefir probiotics. The raw material goat milk of this product has the characteristics of high nutritional value, easy absorption of nutrients and low allergenicity. It has the characteristics of lowering cholesterol, antibacterial and anticancer. The product of the invention has high protein content, moderate sweetness, low goat milk smell and unique flavor, and also has the characteristics of delicate texture, uniform structure, beautiful color and good taste.
本发明解决了现有技术添加果汁造成发酵乳制品质量下降的难题,为浓缩果汁与搅拌型羊奶发酵制品的结合提供了一个新的途径。在果味搅拌型羊奶开菲尔生产工艺方面,具有低成本、高稳定性与感官特性的特点。The invention solves the problem in the prior art that the quality of the fermented milk product is lowered due to the addition of fruit juice, and provides a new way for the combination of the concentrated fruit juice and the stirred goat milk fermented product. In terms of the production process of the fruity stirred goat milk kefir, it has the characteristics of low cost, high stability and sensory properties.
具体实施方式detailed description
下面结合具体实施例,进一步阐述本发明。应理解,实施例仅用于说明本发明而不用于限制本发明的范围。Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that the examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.
实施例1Example 1
本实施例的果汁选择黄桃果汁,并以浓缩比例1:7浓缩为浓缩果汁,具体的浓缩果汁添加量为:若需要制得的果味搅拌型羊奶开菲尔中果汁添加量为液态原料乳体积的15%,液态原料乳按每500mL计,则要添加黄桃果汁75mL,按浓缩比例1:7计算,相当于要添加浓缩黄桃汁10.71mL。The fruit juice in this example is yellow peach juice, and concentrated into concentrated fruit juice at a concentration ratio of 1:7. The specific amount of concentrated fruit juice added is: if the amount of fruit juice added to the prepared fruity stirred goat milk kefir is liquid 15% of the volume of raw milk, liquid raw milk is calculated for every 500mL, 75mL of yellow peach juice should be added, calculated according to the concentration ratio of 1:7, which is equivalent to adding 10.71mL of concentrated yellow peach juice.
聚合乳清蛋白是经过以下步骤制得的:是将10.8g的乳清蛋白分离体(蛋白质含量为92%)溶解于100mL水中,磁力搅拌60min,4℃条件下放置12h,使其充分水合。使用1M的氢氧化钠溶液将乳清蛋白溶液调至pH=7,85℃水浴加热30min,冰水浴冷却,并在4℃下贮藏待用。The polymerized whey protein is prepared through the following steps: 10.8g of whey protein isolate (92% protein content) is dissolved in 100mL of water, magnetically stirred for 60min, and placed at 4°C for 12h to fully hydrate it. The whey protein solution was adjusted to pH = 7 with 1M sodium hydroxide solution, heated in a water bath at 85°C for 30 minutes, cooled in an ice-water bath, and stored at 4°C until use.
果味搅拌型羊奶开菲尔将液态原料乳、糖、果胶、聚合乳清蛋白混合初发酵后,添加浓缩黄桃果汁(浓缩比例为1:7),再经后发酵制得。Fruity Stirred Goat Milk Kefir is made by mixing liquid raw milk, sugar, pectin, and polywhey protein for initial fermentation, adding concentrated yellow peach juice (concentration ratio: 1:7), and then post-fermenting.
本实施例果味搅拌型羊奶开菲尔的制备方法有下列工艺步骤:The preparation method of the fruit flavor stirring type goat milk kefir in this embodiment has the following process steps:
预先混合物加入液态原料乳中混合搅拌→搅拌加热至50℃至果胶充分溶解→加入聚合乳清蛋白→加热至80℃保温10min杀菌→冷却→添加开菲尔发酵剂→25℃发酵至pH低于4.6→破乳→加入浓缩黄桃果汁→分装→4℃下后发酵24h→成品4℃贮藏。Add the pre-mixture to the liquid raw milk and mix and stir → stir and heat to 50°C until the pectin is fully dissolved → add polywhey protein → heat to 80°C for 10 minutes to sterilize → cool → add kefir starter → ferment at 25°C until the pH is low In 4.6 → demulsification → add concentrated yellow peach juice → sub-packaging → post-fermentation at 4°C for 24 hours → store the finished product at 4°C.
具体的,将7.5%(37.5g)白砂糖、0.1%(0.5g)果胶预先混合并加入到液态原料乳(500mL)中搅拌均匀,搅拌加热至50℃至果胶完全溶解,将本实施例制备的聚合乳清蛋白溶液20mL(其中所含的聚合乳清蛋白占液态原料乳的0.4%)加入到原料乳混合液中,搅拌加热至80℃保温10min,之后冷却到25℃,添加0.02%(0.1g)开菲尔发酵剂并搅拌均匀,在25℃初发酵至其pH低于4.6,破乳并搅拌均匀,加入浓缩黄桃汁(10.71mL),待搅拌均匀后分装,于4℃后发酵24h得成品,4℃条件下贮藏。Specifically, 7.5% (37.5g) of white granulated sugar and 0.1% (0.5g) of pectin were pre-mixed and added to liquid raw milk (500mL) and stirred evenly, stirred and heated to 50°C until the pectin was completely dissolved. Add 20mL of the prepared polymerized whey protein solution (the polymerized whey protein contained in it accounts for 0.4% of the liquid raw milk) into the raw milk mixture, stir and heat to 80°C for 10min, then cool to 25°C, add 0.02 % (0.1g) kefir starter and stir evenly, initially ferment at 25°C until its pH is lower than 4.6, break the emulsion and stir evenly, add concentrated yellow peach juice (10.71mL), divide it after stirring evenly, and put it in Ferment at 4°C for 24 hours to obtain the finished product, and store at 4°C.
实施例2Example 2
本实施例的果汁选择草莓果汁,并以浓缩比例1:8浓缩为浓缩果汁,具体的浓缩果汁添加量为:若需要制得的果味搅拌型羊奶开菲尔中果汁添加量为液态原料乳体积的10%,液态原料乳按每500mL计,则要添加草莓果汁50mL,按浓缩比例1:8计算,相当于要添加浓缩草莓汁6.25mL。The fruit juice in this example is strawberry juice, and it is concentrated into concentrated fruit juice at a concentration ratio of 1:8. The specific amount of concentrated fruit juice added is: if the amount of fruit juice added to the prepared fruity-flavored goat milk kefir is liquid raw material For 10% of the milk volume, 50mL of strawberry juice should be added for every 500mL of liquid raw milk, which is equivalent to adding 6.25mL of concentrated strawberry juice according to the concentration ratio of 1:8.
聚合乳清蛋白是经过以下步骤制得的:是将10.8g的乳清蛋白分离体(蛋白质含量为92%)溶解于100mL水中,磁力搅拌60min,4℃条件下放置10h,使其充分水合。使用3M的氢氧化钠溶液将乳清蛋白溶液调至pH=7,80℃水浴加热30min,冰水浴冷却,并在4℃下贮藏待用。The polymerized whey protein is prepared through the following steps: dissolving 10.8g of whey protein isolate (with a protein content of 92%) in 100mL of water, magnetic stirring for 60min, and placing it at 4°C for 10h to fully hydrate it. The whey protein solution was adjusted to pH = 7 with 3M sodium hydroxide solution, heated in a water bath at 80°C for 30 minutes, cooled in an ice-water bath, and stored at 4°C until use.
果味搅拌型羊奶开菲尔将液态原料乳、糖、果胶、聚合乳清蛋白混合预发酵后,添加浓缩草莓果汁(浓缩比例为1:8),再经后发酵制得。Fruity Stirred Goat Milk Kefir is made by mixing liquid raw milk, sugar, pectin, and polywhey protein for pre-fermentation, adding concentrated strawberry juice (the concentration ratio is 1:8), and then post-fermenting.
本实施例果味搅拌型羊奶开菲尔的制备方法是按下列工艺步骤制得:The preparation method of the fruit flavor stirring type goat milk kefir of this embodiment is to make according to the following process steps:
预先混合物加入液态原料乳中溶解并搅拌均匀→加热搅拌至55℃→加入聚合乳清蛋白溶液→加热至85℃保温15min→冷却→添加开菲尔发酵剂→28℃发酵至pH低于4.6→破乳→加入浓缩草莓果汁→分装→4℃下后发酵12h→成品4℃贮藏。Add the premixed liquid raw milk to dissolve and stir well → heat and stir to 55°C → add polywhey protein solution → heat to 85°C and keep warm for 15 minutes → cool → add kefir starter → ferment at 28°C until the pH is lower than 4.6 → Demulsification → adding concentrated strawberry juice → subpackaging → post-fermentation at 4°C for 12 hours → store the finished product at 4°C.
具体的,将7%(35g)白砂糖、0.2%(1g)果胶预先混合并缓慢加入到液态原料乳(500mL)中,搅拌加热至55℃至果胶完全溶解,再将本实施例制备的聚合乳清蛋白溶液15mL(其中所含的聚合乳清蛋白占液态原料乳的0.3%)加入到原料乳混合液中,搅拌加热至85℃保温15min,之后冷却到28℃,添加0.01%(0.05g)开菲尔发酵剂并搅拌均匀,在28℃初发酵至其pH低于4.6,破乳并搅拌均匀,加入浓缩草莓汁(6.25mL),待搅拌均匀后分装,于4℃后发酵12h得成品,4℃条件下贮藏。Specifically, 7% (35g) of white granulated sugar and 0.2% (1g) of pectin were mixed in advance and slowly added to liquid raw milk (500mL), stirred and heated to 55°C until the pectin was completely dissolved, and then prepared in this example 15mL of the polymerized whey protein solution (the polymerized whey protein contained in it accounts for 0.3% of the liquid raw milk) was added to the raw milk mixture, stirred and heated to 85°C for 15min, then cooled to 28°C, and 0.01% ( 0.05g) kefir starter and stir evenly, initially ferment at 28°C until the pH is lower than 4.6, break the emulsion and stir evenly, add concentrated strawberry juice (6.25mL), after stirring evenly, subpackage, after 4°C Ferment for 12 hours to obtain the finished product, and store at 4°C.
实施例3Example 3
本实施例的果汁选择蓝莓果汁,并以浓缩比例1:10浓缩为浓缩果汁,具体的浓缩果汁添加量为:若需要制得的果味搅拌型羊奶开菲尔中果汁添加量为液态原料乳体积的5%,液态原料乳按每500mL计,则要添加蓝莓果汁25mL,按浓缩比例1:10计算,相当于要添加浓缩蓝莓汁2.5mL。The fruit juice in this example is blueberry juice, and it is concentrated into concentrated fruit juice at a concentration ratio of 1:10. The specific amount of concentrated fruit juice added is: if the amount of fruit juice added to the prepared fruity stirred goat milk kefir is liquid raw materials 5% of the milk volume, liquid raw milk is calculated for every 500mL, then 25mL of blueberry juice should be added, calculated according to the concentration ratio of 1:10, which is equivalent to adding 2.5mL of concentrated blueberry juice.
聚合乳清蛋白是经过以下步骤制得的:是将12.5g的浓缩乳清蛋白(蛋白质含量为80%)溶解于100mL水中,磁力搅拌45min,4℃条件下放置10h,使其充分水合。使用1M的氢氧化钠溶液将乳清蛋白溶液调至pH=7,85℃水浴加热30min,冰水浴冷却,并在4℃下贮藏待用。The polymerized whey protein is prepared through the following steps: 12.5g of concentrated whey protein (80% protein content) is dissolved in 100mL of water, magnetically stirred for 45min, and placed at 4°C for 10h to fully hydrate it. The whey protein solution was adjusted to pH = 7 with 1M sodium hydroxide solution, heated in a water bath at 85°C for 30 minutes, cooled in an ice-water bath, and stored at 4°C until use.
果味搅拌型羊奶开菲尔将液态原料乳、糖、果胶、聚合乳清蛋白混合初发酵后,添加浓缩蓝莓果汁(浓缩比例为1:10),再经后发酵制得。Fruity Stirred Goat Milk Kefir is made by mixing liquid raw milk, sugar, pectin, and polywhey protein for initial fermentation, adding concentrated blueberry juice (concentration ratio: 1:10), and then post-fermenting.
本实施例果味搅拌型羊奶开菲尔的制备方法是按下列工艺步骤制得:The preparation method of the fruit flavor stirring type goat milk kefir of this embodiment is to make according to the following process steps:
预先混合物加入液态原料乳中溶解并搅拌均匀→加热搅拌至50℃→加入聚合乳清蛋白溶液→加热至85℃保温15min→冷却→添加开菲尔发酵剂→30℃发酵至pH低于4.6→破乳→加入浓缩蓝莓果汁→分装→4℃下后发酵12h→成品4℃贮藏。Add the pre-mixture into liquid raw milk to dissolve and stir evenly→heat and stir to 50°C→add polywhey protein solution→heat to 85°C and keep warm for 15min→cool→add kefir starter→ferment at 30°C until the pH is lower than 4.6→ Demulsification → adding concentrated blueberry juice → subpackaging → post-fermentation at 4°C for 12 hours → store the finished product at 4°C.
具体的,将6.5%(32.5g)白砂糖和0.3%(1.5g)果胶预先混合并加入到液态原料乳(500mL)中并搅拌均匀,搅拌加热至50℃至果胶完全溶解,加入本实施例制备的聚合乳清蛋白溶液10mL(其中所含的聚合乳清蛋白占液态原料乳的0.2%)到原料乳混合液中,升温至80℃保温10min,之后冷却到31℃,添加0.005%(0.025g)开菲尔发酵剂并搅拌均匀,在31℃初发酵至其pH低于4.6,破乳并搅拌均匀,加入浓缩蓝莓汁2.5mL,待搅拌均匀后分装,于4℃后发酵24h得成品,4℃条件下贮藏。Specifically, 6.5% (32.5g) white granulated sugar and 0.3% (1.5g) pectin are pre-mixed and added to liquid raw milk (500mL) and stirred evenly, stirred and heated to 50°C until the pectin is completely dissolved, and this 10mL of the polymerized whey protein solution prepared in the example (the polymerized whey protein contained in it accounts for 0.2% of the liquid raw milk) was added to the raw milk mixture, the temperature was raised to 80°C and kept for 10min, then cooled to 31°C, and 0.005% (0.025g) kefir starter and stir evenly, initially ferment at 31°C until the pH is lower than 4.6, break the emulsion and stir evenly, add 2.5mL of concentrated blueberry juice, after stirring evenly, subpackage, and post-ferment at 4°C The finished product was obtained within 24 hours and stored at 4°C.
对实施例1~3的产品的分析项目及方法如下:The analysis items and method of the product of embodiment 1~3 are as follows:
(1)理化指标:采用国标方法,分析其总固形物含量、灰分、蛋白质、脂肪、碳水化合物。(1) Physical and chemical indicators: use the national standard method to analyze its total solid content, ash, protein, fat, and carbohydrates.
(2)稳定性:分析其粘度(Brookfield粘度计)、pH、测定脱水收缩率(贝克曼离心机);(2) Stability: analyze its viscosity (Brookfield viscometer), pH, and measure syneresis (Beckman centrifuge);
(3)开菲尔发酵剂:来源于制造商(丹尼斯克公司);(3) Kefir starter: sourced from the manufacturer (Danisco);
(4)感官分析:本发明产品在可接受程度和喜爱度方面进行测试;同时对本发明产品进行描述性分析,按照外观和颜色、味道、风味、组织状态及总体接受性来确定产品的主要特点;所有感官分析是由食品科学专业的12名学生组成的经过训练的专业小组完成;通过使用SPSS 21.0版进行方差分析和卡方检验来进行数据分析。(4) Sensory analysis: the product of the present invention is tested in terms of acceptability and liking; at the same time, the product of the present invention is descriptively analyzed, and the main characteristics of the product are determined according to appearance and color, taste, flavor, tissue state and overall acceptability ; All sensory analyzes were performed by a trained professional team of 12 students in food science; data analysis was performed by ANOVA and chi-square test using SPSS version 21.0.
产品经分析得到的技术指标结果如表1、表2。The technical index results obtained by product analysis are shown in Table 1 and Table 2.
表1产品特性:理化和微生物指标Table 1 Product Characteristics: Physicochemical and Microbiological Indexes
表2产品特点(5分制)Table 2 Product Features (5-point scale)
理化和微生物特性分析结果表明:果味搅拌型羊奶开菲尔蛋白质含量高,粘度高,pH适中,脱水收缩率低。最终产品中嗜酸乳杆菌含量高达1010Log CFU/mL,且未检出大肠杆菌、霉菌和酵母菌等有害菌的存在。The analysis results of physicochemical and microbiological properties showed that the fruity-flavored goat milk kefir had high protein content, high viscosity, moderate pH and low syneresis. The content of Lactobacillus acidophilus in the final product was as high as 10 10 Log CFU/mL, and no harmful bacteria such as Escherichia coli, mold and yeast were detected.
感官评定结果表明:果味搅拌型羊奶开菲尔各项得分均在3分以上,味道浓郁,具有开菲尔特有的风味,且羊奶膻味较低,果味较为明显。产品颜色纯正,质地均匀细腻,可以被认为是一种良好的搅拌型发酵乳制品。The results of the sensory evaluation showed that the fruity-flavored goat's milk kefir had a strong taste with a unique flavor of kefir, and the goat's milk had a low smell and a more obvious fruity taste. The product has a pure color and a uniform and delicate texture, which can be considered as a good stirred fermented dairy product.
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710018973.1A CN106615117A (en) | 2017-01-11 | 2017-01-11 | Stirring type fruit-flavor goat milk kefir and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710018973.1A CN106615117A (en) | 2017-01-11 | 2017-01-11 | Stirring type fruit-flavor goat milk kefir and preparation method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106615117A true CN106615117A (en) | 2017-05-10 |
Family
ID=58842848
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710018973.1A Pending CN106615117A (en) | 2017-01-11 | 2017-01-11 | Stirring type fruit-flavor goat milk kefir and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106615117A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110973257A (en) * | 2019-12-23 | 2020-04-10 | 广州市朴诚乳业有限公司 | Kefir fermented dairy product and preparation method thereof |
CN113832065A (en) * | 2021-10-11 | 2021-12-24 | 上海应用技术大学 | A kind of milk cake starter with the function of producing aroma and inhibiting mutton and its application |
CN114190435A (en) * | 2021-09-17 | 2022-03-18 | 湖州师范学院 | Method for producing new kefir fermented milk by using kefir grains pre-cultured in fruit and vegetable juice |
CN115474632A (en) * | 2022-09-22 | 2022-12-16 | 东北农业大学 | Kefir fermented goat milk containing indigo honeysuckle juice and preparation method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104186661A (en) * | 2014-09-26 | 2014-12-10 | 齐鲁工业大学 | Grape-almond high-calcium goat yogurt fermented milk and preparation method thereof |
CN104886251A (en) * | 2015-05-26 | 2015-09-09 | 福建师范大学闽南科技学院 | Preparation method of banana-goat milk composite yoghourt |
CN105053187A (en) * | 2015-07-24 | 2015-11-18 | 光明乳业股份有限公司 | Fermented milk with flavor of coconuts and preparation method of fermented milk |
CN105053189A (en) * | 2015-07-08 | 2015-11-18 | 南阳师范学院 | Barbury wolfberry fruit coffee composite fermented goat's milk and preparation method thereof |
CN105519670A (en) * | 2015-12-24 | 2016-04-27 | 吉林大学 | Goat milk kefir produced with polymerized lactalbumin as thickener |
-
2017
- 2017-01-11 CN CN201710018973.1A patent/CN106615117A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104186661A (en) * | 2014-09-26 | 2014-12-10 | 齐鲁工业大学 | Grape-almond high-calcium goat yogurt fermented milk and preparation method thereof |
CN104886251A (en) * | 2015-05-26 | 2015-09-09 | 福建师范大学闽南科技学院 | Preparation method of banana-goat milk composite yoghourt |
CN105053189A (en) * | 2015-07-08 | 2015-11-18 | 南阳师范学院 | Barbury wolfberry fruit coffee composite fermented goat's milk and preparation method thereof |
CN105053187A (en) * | 2015-07-24 | 2015-11-18 | 光明乳业股份有限公司 | Fermented milk with flavor of coconuts and preparation method of fermented milk |
CN105519670A (en) * | 2015-12-24 | 2016-04-27 | 吉林大学 | Goat milk kefir produced with polymerized lactalbumin as thickener |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110973257A (en) * | 2019-12-23 | 2020-04-10 | 广州市朴诚乳业有限公司 | Kefir fermented dairy product and preparation method thereof |
CN114190435A (en) * | 2021-09-17 | 2022-03-18 | 湖州师范学院 | Method for producing new kefir fermented milk by using kefir grains pre-cultured in fruit and vegetable juice |
CN114190435B (en) * | 2021-09-17 | 2023-10-31 | 湖州师范学院 | Method for producing kefir fermented milk by fermenting kefir grains pre-cultured by fruit and vegetable juice |
CN113832065A (en) * | 2021-10-11 | 2021-12-24 | 上海应用技术大学 | A kind of milk cake starter with the function of producing aroma and inhibiting mutton and its application |
CN113832065B (en) * | 2021-10-11 | 2023-03-24 | 上海应用技术大学 | Milk cake leavening agent with fragrance producing and mutton smell inhibiting functions and application thereof |
CN115474632A (en) * | 2022-09-22 | 2022-12-16 | 东北农业大学 | Kefir fermented goat milk containing indigo honeysuckle juice and preparation method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103583692B (en) | Symbiotic functional high-protein Greek yogurt utilizing polymeric whey protein as main thickening agent and preparation method thereof | |
CN107212090B (en) | Fermented milk beverage and preparation method thereof | |
CN106616144B (en) | Live bacterium type aerated beverage and preparation method thereof | |
CN102972522B (en) | Probiotic milk and preparation method thereof | |
CN110074193A (en) | A kind of plant base fermented yoghourt stabilizer and its application | |
CN106857834B (en) | Fermented milk with post-acidification inhibiting function and preparation method thereof | |
CN106689964B (en) | Stabilizer for sterilization type fermented protein beverage, sterilization type fermented protein beverage and preparation method | |
CN107136214B (en) | Zero lactose fermented milk and preparation method thereof | |
CN109349354B (en) | Soybean oligopeptide probiotic fermented beverage and preparation method thereof | |
CN103828920B (en) | A kind of coagulating type red colouring agent for food, also used as a Chinese medicine Yoghourt and preparation technology thereof | |
CN104381449A (en) | Yogurt containing fermented glutinous rice and fruit granules and preparation method of yogurt | |
CN107372825A (en) | A kind of blueberry milk beer drink and its production method | |
CN106615117A (en) | Stirring type fruit-flavor goat milk kefir and preparation method thereof | |
JP2018530307A (en) | Plant lactic acid bacteria proliferating agent, fermented product added with the proliferating agent, and preparation method | |
CN105394179A (en) | Cereal kefir beverage and preparation method thereof | |
CN102578229B (en) | Yoghurt containing hippophae rhamnoides | |
CN103749673A (en) | Yak yoghourt and preparation method thereof | |
CN105661230A (en) | Probiotic functional beverage and probiotic food prepared from Lens culinaris by fermentation | |
CN111758780A (en) | Drinking type yoghourt and preparation method thereof | |
CN108185002A (en) | A kind of walnut balance albumen frozen dried sour milk and preparation method thereof | |
CN106387537B (en) | A kind of loquat fermented beverage and preparation method thereof | |
CN108902314B (en) | Double-protein fermented milk beverage rich in active lactobacillus plantarum and preparation method thereof | |
CN104957696A (en) | Production process of probiotic bacteria fermentation type beverage | |
CN113080257A (en) | Buffalo yoghourt, yoghourt beverage and preparation method thereof | |
CN109497133B (en) | Compound proliferation agent capable of delaying reduction of viable count of lactobacillus plantarum fermented milk |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |
|
WD01 | Invention patent application deemed withdrawn after publication |