CN106386866A - Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis - Google Patents

Abstract

The invention discloses a biological pesticide for preventing and controlling corn borer by using Beauveria bassiana and Bacillus thuringiensis. #(volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% urea (mass volume ratio), water supplement 1L composition. The invention combines Beauveria bassiana or Bacillus thuringiensis to create a water suspending agent, uses high-stalk spraying to prevent and control the Asian corn borer in the field, and shortens the insecticidal cycle by 20%. At the same cost as the single application of 1×10 ⁹ concentration of Beauveria bassiana aqueous suspension, the application of 1×10 ⁴ spores/ml of Beauveria bassiana and 1×10 ⁷ spores/ml of Bacillus thuringiensis can jointly prevent and control corn borer indoors The insecticidal cycle is shortened by more than 20%, and the control effect in the field is increased by more than 20%.

Description

A biopesticide for controlling corn borer by using Beauveria bassiana and Bacillus thuringiensis

technical field

The invention belongs to the technical field of corn borer prevention and control, and in particular relates to a biological pesticide for preventing and controlling corn borer by using Beauveria bassiana and Bacillus thuringiensis.

Background technique

Corn is the second largest grain crop in my country, with an annual output of 300 billion catties. It can be used for food, and can also be used as feed and industrial raw materials to produce industrial alcohol and shochu. The corn borer is the main pest in corn production. It not only eats the leaves of corn, bores into the main stem or ear of corn, reduces photosynthesis and affects nutrient transportation; The yield loss of corn is 7%-15%, more than 50% yield reduction in severe years, or even no yield, causing huge economic losses to my country's grain production. Chemical pesticides have always played a major role in the prevention and control of corn borer, ensuring food production and safety issues. However, with the continuous use of chemical pesticides in large quantities, pesticide residues have become more and more harmful to food safety and environmental safety. High-efficiency pesticides with good compatibility have become the direction of pesticide development. At present, the biological control of corn borer in my country mainly uses Beauveria bassiana to control corn borer and Trichogramma parasitism. Beauveria bassiana is a filamentous fungus and is currently the most widely used entomopathogenic fungus in China. Beauveria bassiana has the advantages of strong pathogenicity, wide insecticidal range, and environmental friendliness. However, there are still shortcomings such as single dosage form, low labor efficiency, slow insecticidal speed, and low insecticidal efficiency in the control. At present, the most successful promotion model of Beauveria bassiana in my country is the control of corn borer and pine caterpillar, but they are all promoted by the government. Bacteria granules are also used, but the labor efficiency is low and has gradually disappeared. The main reasons for the difficulty in the promotion and commercialization of Beauveria bassiana in my country are the degradation of strain virulence, unstable spore production and single dosage form. In recent years, the isolation and application of Bacillus thuringiensis strains have been strengthened in various countries, and they have been widely used in various Lepidoptera and Coleoptera pests such as Spodoptera exigua (Hübner), Spodoptera litura (Fabricius) , Dendrolimus punctatus (Walker), Ectropis obliqua (Prout), Chilo suppressalis (Walker) (Li Qing et al., 2009; ), Aedes albopictus (Zhang Lingling et al., 2008), grubs, etc. prevention and treatment. However, there are few studies on the application of Bacillus thuringiensis strains for the control of corn borer.

At present, Beauveria bassiana or Bacillus thuringiensis used alone to control corn borer has long insecticidal cycle, easy to produce resistance, and high cost.

Contents of the invention

The object of the present invention is to provide a kind of biological pesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, aiming to solve the problem that current Beauveria bassiana or Bacillus thuringiensis used alone to prevent and control corn borer has long killing cycle and easy to produce resistance , the problem of higher cost.

The present invention is achieved in this way, a kind of biological pesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, and the biological pesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer consists of 0.1g Beauveria bassiana (D1 -5 strain) high sporopollen (spore density is 1×10 ¹¹ /g), 10 g Bacillus thuringiensis (JN001 strain) high sporopollen (spore density is 1×10 ¹² /g), 6% additive 1 (volume ratio )#, 2% additive 2# (volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% urea (mass volume ratio), and water to make up 1L.

Further, the water suspension of the biopesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer is diluted 1000 times, the final concentration of Beauveria bassiana spores is ¹ ×104 spores/ml, and the final concentration of Bacillus thuringiensis is 1 ×10 ⁷ spores/ml.

Another object of the present invention is to provide a kind of preparation method of the biopesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, and the preparation method comprises the following steps:

Take 0.1g of Beauveria bassiana (strain D1-5) high sporopollen (spore density of 1×10 ¹¹ /g), 10 g of Bacillus thuringiensis (JN001 strain) high sporopollen (spore density of 1×10 ¹² /g) ), 6% auxiliary agent 1 (volume ratio)#, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% urea (mass volume ratio) , after fully grinding and mixing, add sterile water to make up 1L, and seal and store.

Further, the method of using the biological pesticide for controlling corn borer with Beauveria bassiana and Bacillus thuringiensis comprises:

Dilute the prepared biopesticide aqueous suspension by 1000 times, and use a high pole sprayer to carry out field spraying control at the horn-mouth stage of corn in mid-June, with 20 L per mu.

The present invention provides a biopesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, combines Beauveria bassiana or Bacillus thuringiensis to create a water suspension, and uses high-stalk spraying to prevent and control the Asian corn borer in the field, and the insecticidal cycle 20% shorter. Beauveria bassiana and Bacillus thuringiensis combined prevention and control of field pests have been reported so far, mainly wettable powder to control canopy caterpillars, liquid preparations to control distiller's yeast pests, such as rice robbers, etc. There is no related research on the use of the two combined water suspensions to control corn borer. See report. At the same cost as the single application of 1×10 ⁹ concentration of Beauveria bassiana aqueous suspension, the application of 1×10 ⁴ spores/ml of Beauveria bassiana and 1×10 ⁷ spores/ml of Bacillus thuringiensis can jointly prevent and control corn borer indoors The insecticidal cycle is shortened by more than 20%, and the field control effect is increased by more than 20%;

Field application: Dilute the prepared water-suspension agent 1000 times, and use a high-stem sprayer to carry out field spraying control in the horn-mouth stage of corn (mid-June), 20L per mu;

In the second ten days of September, the insect population decline rate was investigated by dissection. Taking the untreated plot as a control, the combined control effect of Beauveria bassiana and Bacillus thuringiensis reached 85.06%, and the control effect of Beauveria bassiana alone was 66.67%, and the control effect increased by 21.62%.

Indoor toxicity test: Dilute the prepared water suspension 1000 times, the final concentration of Beauveria bassiana spores is 1×10 ⁴ spores/ml, the final concentration of Bacillus thuringiensis is 1×10 ⁷ spores/ml, use the dipping method to The 2nd instar larvae of the Asian corn borer were tested for indoor virulence. ^The blank control was diluted 1000 times with no Beauveria bassiana and Bacillus thuringiensis suspension additive respectively. /ml is the control. The results of toxicity test showed that the LT90 of Beauveria bassiana and Bacillus thuringiensis aqueous suspension was 7.05 days, and the LT90 of Beauveria bassiana alone was 8.98 days, and the insecticidal time was shortened by 21.49%.

Description of drawings

Fig. 1 is a flowchart of a preparation method of a biopesticide for controlling corn borer by using Beauveria bassiana and Bacillus thuringiensis provided by an embodiment of the present invention.

detailed description

In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

The embodiment of the present invention utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control the biological pesticide of corn borer by 0.1g Beauveria bassiana (D1-5 bacterial strain) high sporopollen (spore density is 1×10 ¹¹ /g), 10g Bacillus thuringiensis Bacillus (JN001 strain) high sporopollen (spore density is 1×10 ¹² /g), 6% auxiliary agent 1 (volume ratio)#, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume ratio ), 0.05% formaldehyde (volume ratio), 5% urea (mass volume ratio), and water to make up 1L composition. .

As shown in Figure 1, the preparation method of the biopesticide utilizing Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer in the embodiment of the present invention comprises the following steps:

S101: Take 0.1 g of Beauveria bassiana (strain D1-5) high sporopollen (spore density of 1×10 ¹¹ /g), and 10 g of Bacillus thuringiensis (JN001 strain) high sporopollen (spore density of 1×10 ¹² ) /g), 6% auxiliary agent 1 (volume ratio)#, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% urea (mass volume Compare);

S102: After fully grinding and mixing, add sterile water to make up 1L, and keep it sealed.

The application effects of the present invention will be described in detail below in conjunction with specific embodiments.

Example 1:

Indoor toxicity test: Dilute the prepared water suspension 1000 times, the final concentration of Beauveria bassiana spores is 1×10 ⁴ spores/ml, the final concentration of Bacillus thuringiensis is 1×10 ⁷ spores/ml, use the dipping method to The 2nd instar larvae of the Asian corn borer were tested for indoor virulence. ^The blank control was diluted 1000 times with no Beauveria bassiana and Bacillus thuringiensis suspension additive respectively. /ml is the control. The results of toxicity test showed that the LT90 of Beauveria bassiana and Bacillus thuringiensis aqueous suspension was 7.05 days, and the LT90 of Beauveria bassiana alone was 8.98 days, and the insecticidal time was shortened by 21.49%.

Example 2:

Field application: Dilute the prepared water-suspension agent 1000 times, and use a high-stem sprayer to carry out field spraying and control at the bell mouth stage of corn (mid-June), 20L per mu. In the second ten days of September, the insect population decline rate was investigated by dissection. Taking the untreated plot as a control, the combined control effect of Beauveria bassiana and Bacillus thuringiensis reached 85.06%, and the control effect of Beauveria bassiana alone was 66.67%, and the control effect increased by 21.62%.

The application effects of the present invention will be described in detail below in conjunction with comparison.

At the same cost as the single application of 1×10 ⁹ concentration of Beauveria bassiana aqueous suspension, the application of 1×10 ⁴ spores/ml of Beauveria bassiana and 1×10 ⁷ spores/ml of Bacillus thuringiensis can jointly prevent and control corn borer indoors The insecticidal cycle is shortened by more than 20%, and the control effect in the field is increased by more than 20%.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.

Claims (4)

1. a biological pesticide utilizing Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, characterized in that, the biological pesticide utilizing Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer consists of 0.1g Beauveria bassiana, 10g thuringiensis Bacillus, 6% additive 1#, 2% additive 2#, 0.05% xanthan gum, 0.05% formaldehyde, 5% urea, water; Among them, 6% auxiliary agent 1#, 2% auxiliary agent 2#, 0.05% xanthan gum, and 0.05% formaldehyde are all in volume ratio; 5% urea is in mass volume ratio; The total amount of biopesticide for corn borer is 1L. 2. utilize the biological pesticide of Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer as claimed in claim 1, it is characterized in that, the water suspension agent dilution of the biological pesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer 1000 times, the final concentration of Beauveria bassiana spores is 1×10 ⁴ spores/ml, and the final concentration of Bacillus thuringiensis is 1×10 ⁷ spores/ml. 3. a preparation method utilizing Beauveria bassiana and Bacillus thuringiensis to prevent and control the biological pesticide of corn borer as claimed in claim 1, it is characterized in that, described preparation method comprises the following steps: Take 0.1g Beauveria bassiana high sporopollen, 10g Bacillus thuringiensis, 6% additive 1#, 2% additive 2#, 0.05% xanthan gum, 0.05% formaldehyde, 5% urea, grind and mix well , add sterile water to make up 1L, and keep it sealed. 4. as claimed in claim 3, utilize Beauveria bassiana and Bacillus thuringiensis to prevent and control the preparation method of the biological pesticide of corn borer, it is characterized in that, described use of the biological pesticide of Beauveria bassiana and Bacillus thuringiensis prevention and control corn borer Methods include: Dilute the prepared biopesticide aqueous suspension by 1000 times, and use a high pole sprayer to carry out field spraying control at the horn-mouth stage of corn in mid-June, with 20 L per mu.