CN106053362A - Absorbance detection system and method - Google Patents
Absorbance detection system and method Download PDFInfo
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- CN106053362A CN106053362A CN201610345353.4A CN201610345353A CN106053362A CN 106053362 A CN106053362 A CN 106053362A CN 201610345353 A CN201610345353 A CN 201610345353A CN 106053362 A CN106053362 A CN 106053362A
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- 238000002835 absorbance Methods 0.000 title claims abstract description 35
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 185
- 238000001228 spectrum Methods 0.000 claims abstract description 77
- 230000003321 amplification Effects 0.000 claims abstract description 56
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 56
- 239000000126 substance Substances 0.000 claims abstract description 25
- 239000002131 composite material Substances 0.000 claims abstract description 13
- 230000003287 optical effect Effects 0.000 claims description 39
- 239000013307 optical fiber Substances 0.000 claims description 30
- 238000001914 filtration Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 description 10
- 238000010586 diagram Methods 0.000 description 9
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/128—Alternating sample and standard or reference part in one path
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Abstract
The invention provides an absorbance detection system and method. The system comprises a light filter selecting module, which is used to switch light filters and filter the composite light emitted by a light source to obtain single color light that is matched with the test task type; at least one test channel, which is used to transmit single color light that penetrates a test substance; at least one reference channel, which is used to transmit single color light that is matched with the test task type; a collection module, which is used to collect a first light intensity value of single color light that penetrate at least one test channel and a second light intensity value of single color light that is transmitted by at least one reference channel; and a microprocessor, which is used to calculate the absorbance of the test substance according to the first light intensity value and the second light intensity value. The system is provided with a plurality of reference channels, the amplification number of each reference channel is identical with the amplification number of the corresponding test channels of corresponding spectrum collection task so as to ensure that the amplification number of the first light intensity value is equal to the amplification number of the second light intensity valve, thus the error of calculated absorbance error is reduced, and the detection precision is improved.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to an absorbance detection system and method.
Background
In the existing blood coagulation tester, as shown in fig. 1, the light filter 2 filters the composite light of the light source 1 into monochromatic light, the light splitter 3 divides the monochromatic light into a plurality of beams, the beams are transmitted to the test site 5 through the incident optical fiber 4, the monochromatic light transmitted through the test substance in the test site 5 is transmitted to the acquisition module 7 through the receiving optical fiber 6, the acquisition module 7 converts the monochromatic light into a first electrical signal, the first electrical signal is amplified by using the amplification factor corresponding to the monochromatic light, the amplified first electrical signal is transmitted to the microprocessor, and the microprocessor converts the amplified first electrical signal into a light intensity value which is used as transmission light intensity; the reference optical fiber 8 directly transmits the monochromatic light to the acquisition module 7, the acquisition module 7 converts the monochromatic light into a second electric signal, amplifies the second electric signal by using a preset amplification factor, sends the amplified second electric signal to the microprocessor, converts the amplified second electric signal into a light intensity value serving as incident light intensity, and finally, the microprocessor calculates the absorbance of the test substance by using the incident light intensity and the transmitted light intensity. Because of the influence of the numerical aperture of the optical fiber, the monochromatic light emitted by the incident optical fiber 4 directly irradiates the test site 5, and after the monochromatic light transmits through the test substance in the test site 5, background light is generated, the interference is generated to the monochromatic light received by the receiving optical fiber 6, which causes the error of the transmitted light intensity received by the collecting module 7, and the reference optical fiber 8 directly transmits the monochromatic light to the collecting module 7, which lacks the testing position, so that the incident light intensity of the monochromatic light received by the collection module 7 is much stronger than the transmitted light of the monochromatic light transmitted through the test substance, moreover, after monochromatic light of any spectrum is transmitted to the acquisition module 7 through the reference optical fiber 8, the passing amplification factors are the same, so that the passing amplification factors of the transmitted light intensity and the incident light intensity are inconsistent, a large error is introduced when the absorbance is calculated, and the detection precision is reduced.
Disclosure of Invention
In view of this, the present application provides an absorbance detection system and method to solve the problems of large error and low accuracy in the existing detection method.
According to a first aspect of embodiments herein, there is provided an absorbance detection system, the system comprising: the device comprises a light source, an optical filter chip selection module, at least one reference channel, at least one test channel, an acquisition module and a microprocessor, wherein the acquisition module is respectively connected with the at least one test channel and the at least one reference channel, and the microprocessor is respectively connected with the optical filter chip selection module and the acquisition module; wherein,
the optical filter chip selection module is used for switching an optical filter and filtering the composite light emitted by the light source into monochromatic light matched with the type of the test task;
the at least one test channel is used for transmitting monochromatic light which transmits the test substance;
the at least one reference channel is used for transmitting the monochromatic light matched with the type of the test task;
the acquisition module is used for acquiring a first light intensity value of the monochromatic light transmitted through the at least one test channel and acquiring a second light intensity value of the monochromatic light transmitted through the at least one reference channel;
the microprocessor is used for selecting the optical filter in the optical filter chip selection module according to a preset test task type so that the optical filter filters the composite light emitted by the light source into monochromatic light matched with the test task type; selecting a test channel corresponding to the spectrum acquisition task with the type matched with that of the test task according to a preset spectrum acquisition task corresponding to each test channel in the at least one test channel, and acquiring a first light intensity value of monochromatic light transmitted through the test channel through the acquisition module; selecting a reference channel corresponding to the spectrum acquisition task matched with the type of the test task according to a preset spectrum acquisition task corresponding to each reference channel in the at least one reference channel, and acquiring a second light intensity value of the monochromatic light transmitted through the reference channel through the acquisition module; and calculating the absorbance of the test substance by using the first light intensity value and the second light intensity value.
According to a second aspect of embodiments of the present application, there is provided an absorbance detection method, the method including:
the microprocessor selects an optical filter in the optical filter chip selection module according to a preset test task type, so that the optical filter filters the composite light emitted by the light source into monochromatic light matched with the test task type;
the microprocessor selects a test channel corresponding to the spectrum acquisition task matched with the type of the test task according to the spectrum acquisition task corresponding to each test channel in at least one preset test channel, and acquires a first light intensity value of monochromatic light transmitted through the test channel through an acquisition module;
the microprocessor selects a reference channel corresponding to the spectrum acquisition task matched with the type of the test task according to the spectrum acquisition task corresponding to each preset reference channel in at least one reference channel, and acquires a second light intensity value of the monochromatic light transmitted through the reference channel through the acquisition module;
and the microprocessor calculates the absorbance of the test substance in the test channel by using the first light intensity value and the second light intensity value.
By applying the embodiment of the application, each spectrum acquisition task corresponds to one reference channel, the amplification factor of the gain module connected with each reference channel is consistent with that of the gain chip selection module connected with the test channel corresponding to the corresponding spectrum acquisition task, so that the passing amplification factor of the first light intensity value acquired by the acquisition module is consistent with that of the second light intensity value, the error of absorbance calculation can be reduced, and the detection precision is improved. In addition, the optical path structures of the reference channel and the test channel are the same, namely the reference optical fiber does not directly transmit monochromatic light to the acquisition module, but transmits the monochromatic light to the acquisition module through the incident optical fiber, and the number of layers of the passing diaphragms is the same, so that the consistency of light intensity can be ensured, the error of absorbance calculation can be reduced, and the detection precision is improved.
Drawings
FIG. 1 is a block diagram of a prior art absorbance detection system according to one exemplary embodiment of the present application;
FIG. 2A is a block diagram of an absorbance detection system according to an exemplary embodiment of the present application;
FIG. 2B is a block diagram of a gain chip select module corresponding to each test channel in the embodiment shown in FIG. 2A;
FIG. 2C is a block diagram of a gain module corresponding to each reference channel in the embodiment shown in FIG. 2A;
FIG. 2D is a block diagram of the test channel and the reference channel in the embodiment shown in FIG. 2A;
fig. 3 is a flowchart illustrating an embodiment of an absorbance detection method according to an exemplary embodiment of the present application.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.
It is to be understood that although the terms first, second, third, etc. may be used herein to describe various information, such information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present application. The word "if" as used herein may be interpreted as "at … …" or "when … …" or "in response to a determination", depending on the context.
Fig. 2A is a block diagram of an absorbance detection system according to an exemplary embodiment of the present application, and as shown in fig. 2A, the system may include: the device comprises a light source 10, a light filter chip selection module 20, at least one test channel 30, at least one reference channel 40, an acquisition module 50 and a microprocessor 60, wherein the acquisition module 50 is respectively connected with the at least one test channel 30 and the at least one reference channel 40, and the microprocessor 60 is respectively connected with the light filter chip selection module 20 and the acquisition module 50.
The optical filter chip selection module 20 is used for switching optical filters and filtering the composite light emitted by the light source 10 into monochromatic light matched with the type of the test task;
at least one test channel 30 for transmitting monochromatic light transmitted through the test substance;
at least one reference channel 40 for transmitting monochromatic light matched with the type of the test task;
the acquisition module 50 is used for acquiring a first light intensity value of the monochromatic light transmitted through the at least one test channel 30 and acquiring a second light intensity value of the monochromatic light transmitted through the at least one reference channel 40;
the microprocessor 60 is used for selecting the optical filter in the optical filter chip selection module 20 according to a preset test task type, so that the optical filter filters the composite light emitted by the light source 10 into monochromatic light matched with the test task type; selecting a test channel corresponding to the spectrum acquisition task with the type matched with the type of the test task according to the preset spectrum acquisition task corresponding to each test channel in at least one test channel 30, and acquiring a first light intensity value of monochromatic light transmitted through the test channel through an acquisition module 50; selecting a reference channel corresponding to the spectrum acquisition task with the type matched with the test task type according to the preset spectrum acquisition task corresponding to each reference channel in at least one reference channel 40, and acquiring a second light intensity value of the monochromatic light transmitted through the reference channel through an acquisition module 50; and calculating the absorbance of the test substance by using the first light intensity value and the second light intensity value.
The light source 10 may be a small-angle halogen lamp, which can increase the light intensity and emit composite light closer to parallel light, for example, a 24-degree light-emitting-angle halogen lamp; the optical filter selecting module 20 may be a rotary disk type, which switches the optical filters by rotating the rotary disk, or a linear displacement type, which switches the optical filters by moving.
In an embodiment, the system may further include at least one gain chip selection module connected to the acquisition module 50, each of the at least one gain chip selection module being connected to one of the at least one test channel; the microprocessor 60 may comprise a setting unit for setting each of the at least one test channel as a spectrum acquisition task corresponding to the test channel and setting the amplification of the gain chip select module connected to the test channel as an amplification matching the spectrum acquisition task.
Fig. 2B is a structural diagram of the gain chip select module corresponding to each test channel shown on the basis of the embodiment shown in fig. 2A, and as shown in fig. 2B, the number of the test channels may be set according to a preset test task type, for example, the preset test task type includes a first test task type, a second test task type, and a third test task type, and correspondingly, at least one test channel 30 may include a first test channel 301, a second test channel 302, and a third test channel 303. The spectrum acquisition task can be set according to a preset test task type, for example, the first test task type corresponds to a red light spectrum acquisition task (the wavelength range of red light is 770-622 nm), and the magnification matched with the red light spectrum acquisition task is a times; the second test task type corresponds to a purple light spectrum acquisition task (the wavelength range of purple light is 455-350 nanometers), and the magnification matched with the purple light spectrum acquisition task is b times; the third test task type corresponds to the green spectrum collection task (the wavelength range of green light is 577 nm-492 nm), and the magnification matched with the green spectrum collection task is c times.
As shown in fig. 2B, the first gain chip selection module 701 is connected to the first test channel 301, the second gain chip selection module 702 is connected to the second test channel 302, the third gain chip selection module 703 is connected to the third test channel 303, and each gain chip selection module includes a first amplification factor, a second amplification factor, and a third amplification factor which are selectable, for example, the setting unit of the microprocessor 60 may set the first test channel 301 as a red light spectrum collection task, and set the amplification factor of the first gain chip selection module 701 as a first amplification factor (the amplification factor is a times) matched with the red light spectrum collection task; setting the second test channel 302 as a task of collecting the violet light spectrum, and setting the amplification factor of the second gain chip selection module 702 as a second amplification factor (the amplification factor is b times) matched with the task of collecting the violet light spectrum; the third test channel 303 is set to the green spectrum acquisition task, and the amplification factor of the third gain chip selection module 703 is set to a third amplification factor (the amplification factor is c times) that matches the green spectrum acquisition task.
Based on the above description, the system is provided with a plurality of test channels, and because the gain chip selection module corresponding to each test channel comprises the amplification factor matched with different spectrum acquisition tasks, the amplification factor can be set according to the spectrum acquisition task corresponding to the test task type, so that the system can realize the absorbance detection of various test task types, and meanwhile, the amplification factor required by the spectrum acquisition task corresponding to each test task type can also be realized.
In one embodiment, the system may further include at least one gain module coupled to the acquisition module 50, each of the at least one gain module coupled to one of the at least one reference channel; the setting unit of the microprocessor 60 may be further configured to set each of the at least one reference channel to a spectrum acquisition task corresponding to the reference channel.
Fig. 2C is a structural diagram of a gain module corresponding to each reference channel shown on the basis of the embodiment shown in fig. 2A, as shown in fig. 2C, the number of reference channels is set according to the number of spectrum collection tasks, for example, the spectrum collection tasks include a red spectrum collection task, a violet spectrum collection task, and a green spectrum collection task, correspondingly, at least one reference channel 40 may include a first reference channel 401, a second reference channel 402, and a third reference channel 403, and the amplification factor of the gain module connected to each reference channel matches with the amplification factor corresponding to the spectrum collection task.
As shown in fig. 2C, the first gain module 801 is connected to the first reference channel 401, the second gain module 802 is connected to the second reference channel 402, the third gain module 803 is connected to the third reference channel 403, and the amplification factor of the first gain module 801 is a first amplification factor, which is matched with the amplification factor corresponding to the red light spectrum collection task, and the microprocessor 60 can set the first reference channel 801 as the red light spectrum collection task; the amplification factor of the second gain module 802 is a second amplification factor, and is matched with the amplification factor corresponding to the violet spectrum acquisition task, and the microprocessor 60 can set the second reference channel 402 as the violet spectrum acquisition task; the third gain module 803 has a third amplification factor that matches the amplification factor corresponding to the green spectrum acquisition task, and the microprocessor 60 can set the third reference channel 403 to the green spectrum acquisition task.
Based on the above description, it can be known that the number of the reference channels is set according to the number of the spectrum acquisition tasks, and each reference channel corresponds to one spectrum acquisition task, so that the amplification factor in the gain module connected to each reference channel may be the amplification factor matched with the corresponding spectrum acquisition task, and the amplification factor of the gain chip selection module connected to the test channel corresponding to the spectrum acquisition task is consistent, so that the amplification factor passed by the first light intensity value acquired by the acquisition module 50 is also consistent with the amplification factor passed by the second light intensity value acquired, the error of absorbance calculation can be reduced, and the detection precision can be improved.
In an embodiment, a photoelectric device is arranged between each test channel and the gain chip selection module and between each reference channel and the gain module, and the photoelectric device is used for receiving monochromatic light transmitted by the test channel or the reference channel, converting the received monochromatic light into an electric signal, and then amplifying the electric signal by the gain chip selection module or the gain module.
In an embodiment, fig. 2D is a structural diagram of the test channel and the reference channel in the embodiment shown in fig. 2A, as shown in fig. 2D, each of the at least one test channel 30 includes an incident optical fiber 304 and a test site 305 for containing a test substance, a first diaphragm 306 with a first predetermined number of layers is disposed on a light incident surface of the test site 305, a second diaphragm 307 with a second predetermined number of layers is disposed on a light emergent surface of the test site 305, and the second diaphragm 307 is connected to one gain chip selection module of the at least one gain chip selection module; an incident optical fiber 304 for transmitting monochromatic light matched with the type of the test task; a first diaphragm 306 for receiving the monochromatic light transmitted from the incident optical fiber 304; a second aperture 307 for receiving monochromatic light transmitted through the test substance in the test site 305.
The incident optical fiber 304 may adopt an optical fiber with a smaller numerical aperture, for example, an optical fiber with a numerical aperture of 0.22, to reduce a divergence angle of light emitted from the optical fiber, and the first diaphragm 306 may ensure consistency of the test channel and may reduce stray light except for the parallel light, so that the monochromatic light approaches the parallel light, thereby reducing background light of the monochromatic light after the monochromatic light transmits the test substance in the test site 305; the second diaphragm 307 can further absorb stray light other than the parallel light, so that the monochromatic light finally reaching the collection module 50 is closer to the parallel light. The first preset number of layers may be multiple layers, and the second preset number of layers may also be multiple layers, and considering the complexity of the structure and the cost of the instrument in the process of assembling the instrument, the first preset number of layers may be one layer, and the second preset number of layers may be two layers.
In an embodiment, as further shown in fig. 2D, each of the at least one reference channel 40 includes a reference fiber 404, a first predetermined number of third diaphragms 405, and a second predetermined number of fourth diaphragms 406, and the fourth diaphragms 406 are connected to one of the at least one gain module; a reference optical fiber 404 for transmitting monochromatic light matched with the test task type; a third diaphragm 405, configured to receive monochromatic light transmitted by the reference fiber 404; and a fourth diaphragm 407 for receiving the monochromatic light transmitted through the third diaphragm 405.
In order to ensure the consistency between the test channel and the reference channel, the reference fiber 404 may use a fiber with the same numerical aperture as the incident fiber 304, an optical path with the same size as the test bit 305 may be disposed between the third diaphragm 405 and the fourth diaphragm 406, the number of layers of the third diaphragm 405 may be the same as the number of layers of the first diaphragm 306, and the number of layers of the fourth diaphragm 406 may be the same as the number of layers of the second diaphragm 307.
Based on the above description, the optical path structures of the reference channel and the test channel are the same, that is, the reference fiber 404 does not directly transmit monochromatic light to the acquisition module 50, but the optical path length of the reference fiber is the same as that of the incident fiber 304, and the number of layers of the passing diaphragms is also the same, so that the consistency of light intensity can be ensured, the error of absorbance calculation is reduced, and the detection accuracy is improved.
In one embodiment, as further shown in fig. 2D, the system may further include a light channel 90 and a light splitter 100, the light channel 90 being used for filtering the high-angle light of the composite light emitted from the light source 10 to reduce stray light, and the light splitter 100 being used for splitting the single-color light into multiple beams.
In one embodiment, the microprocessor 60 may further include a calculating unit for obtaining a ratio of the second light intensity value to the first light intensity value; the logarithm of the ratio to the base of the predetermined value is determined as the absorbance of the test substance.
It can be seen from the above embodiments that, each spectrum collection task corresponds to one reference channel, and the amplification factor of the gain module connected to each reference channel is consistent with the amplification factor of the gain chip selection module connected to the test channel corresponding to the corresponding spectrum collection task, so as to ensure that the amplification factor through which the first light intensity value collected by the collection module 50 passes is consistent with the amplification factor through which the second light intensity value passes, thereby reducing the error of absorbance calculation and improving the detection accuracy. In addition, the optical path structures of the reference channel and the test channel are the same, that is, the reference fiber 404 does not directly transmit monochromatic light to the acquisition module 50, but has the same optical path length as the incident fiber 304 transmits monochromatic light, and the number of layers of the passing diaphragm is the same, so that the consistency of light intensity can be ensured, the error of absorbance calculation can be reduced, and the detection precision can be improved.
Fig. 3 is a flowchart illustrating an embodiment of an absorbance detection method according to an exemplary embodiment of the present application, where the embodiment includes the following steps:
step 301: the microprocessor selects an optical filter in the optical filter chip selection module according to a preset test task type so that the optical filter filters the composite light emitted by the light source into monochromatic light matched with the test task type;
step 302: the microprocessor selects a test channel corresponding to the spectrum acquisition task with the type matched with the type of the test task according to the spectrum acquisition task corresponding to each test channel in at least one preset test channel, and acquires a first light intensity value of monochromatic light transmitted through the test channel through an acquisition module;
step 303: the microprocessor selects a reference channel corresponding to the spectrum acquisition task matched with the type of the test task according to the spectrum acquisition task corresponding to each preset reference channel in at least one reference channel, and acquires a second light intensity value of the monochromatic light transmitted through the reference channel through the acquisition module;
step 304: and the microprocessor calculates the absorbance of the test substance in the test channel by using the first light intensity value and the second light intensity value.
Specifically, the microprocessor may obtain a ratio of the second light intensity value to the first light intensity value, and determine a logarithm of the ratio based on a predetermined value as the absorbance of the test substance in the test channel.
The flow described in step 301 to step 303 may refer to the related description of the embodiments shown in fig. 2A to fig. 2D, and is not described again.
According to the embodiment, each spectrum acquisition task corresponds to one reference channel, and the amplification factor of the gain module connected with each reference channel is consistent with that of the gain chip selection module connected with the test channel corresponding to the corresponding spectrum acquisition task, so that the passing amplification factor of the first light intensity value acquired by the acquisition module is consistent with that of the second light intensity value, the error of absorbance calculation can be reduced, and the detection precision is improved. In addition, the optical path structures of the reference channel and the test channel are the same, namely the reference optical fiber does not directly transmit monochromatic light to the acquisition module, but transmits the monochromatic light to the acquisition module through the incident optical fiber, and the number of layers of the passing diaphragms is the same, so that the consistency of light intensity can be ensured, the error of absorbance calculation can be reduced, and the detection precision is improved.
The microprocessor in the embodiment of the present application can be implemented by a hardware circuit according to the design requirement of an actual circuit. It can be clearly understood by those skilled in the art that, for convenience and brevity of description, the specific working process of the method embodiment described above may refer to the corresponding process in the foregoing system embodiment, and is not described herein again.
Those of ordinary skill in the art will understand that: all or part of the steps for implementing the embodiments can be implemented by hardware related to program instructions, and the program can be stored in a computer readable storage medium, and when executed, the program performs the steps including the method embodiments; and the aforementioned storage medium includes: various media that can store program codes, such as ROM, RAM, magnetic or optical disks.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (10)
1. An absorbance detection system, comprising: the device comprises a light source, an optical filter chip selection module, at least one reference channel, at least one test channel, an acquisition module and a microprocessor, wherein the acquisition module is respectively connected with the at least one test channel and the at least one reference channel, and the microprocessor is respectively connected with the optical filter chip selection module and the acquisition module; wherein,
the optical filter chip selection module is used for switching an optical filter and filtering the composite light emitted by the light source into monochromatic light matched with the type of the test task;
the at least one test channel is used for transmitting monochromatic light which transmits the test substance;
the at least one reference channel is used for transmitting the monochromatic light matched with the type of the test task;
the acquisition module is used for acquiring a first light intensity value of the monochromatic light transmitted through the at least one test channel and acquiring a second light intensity value of the monochromatic light transmitted through the at least one reference channel;
the microprocessor is used for selecting the optical filter in the optical filter chip selection module according to a preset test task type so that the optical filter filters the composite light emitted by the light source into monochromatic light matched with the test task type; selecting a test channel corresponding to the spectrum acquisition task with the type matched with that of the test task according to a preset spectrum acquisition task corresponding to each test channel in the at least one test channel, and acquiring a first light intensity value of monochromatic light transmitted through the test channel through the acquisition module; selecting a reference channel corresponding to the spectrum acquisition task matched with the type of the test task according to a preset spectrum acquisition task corresponding to each reference channel in the at least one reference channel, and acquiring a second light intensity value of the monochromatic light transmitted through the reference channel through the acquisition module; and calculating the absorbance of the test substance by using the first light intensity value and the second light intensity value.
2. The system of claim 1, further comprising:
each gain chip selection module in the at least one gain chip selection module is connected with one test channel in the at least one test channel;
the microprocessor includes: and the setting unit is used for setting each test channel in the at least one test channel as a spectrum acquisition task corresponding to the test channel and setting the amplification factor of the gain chip selection module connected with the test channel as the amplification factor matched with the spectrum acquisition task.
3. The system of claim 2, further comprising:
at least one gain module connected to the acquisition module, each of the at least one gain module connected to one of the at least one reference channel;
the setting unit is further configured to set each of the at least one reference channel as a spectrum acquisition task corresponding to the reference channel; and the respective amplification factor of the at least one gain module is matched with the amplification factor corresponding to the spectrum acquisition task.
4. The system of claim 2, wherein each of the at least one test channel comprises: the device comprises an incident optical fiber and a test position for containing test substances, wherein a first diaphragm with a first preset layer number is arranged on a light incident surface of the test position, a second diaphragm with a second preset layer number is arranged on a light emergent surface of the test position, and the second diaphragm is connected with one gain chip selection module in at least one gain chip selection module; wherein,
the incident optical fiber is used for transmitting monochromatic light matched with the type of the test task;
the first diaphragm is used for receiving monochromatic light transmitted by the incident optical fiber;
the second diaphragm is used for receiving the monochromatic light transmitted through the test substance.
5. The system of claim 4, wherein each of the at least one reference channel comprises: the reference optical fiber, a third diaphragm with a first preset number of layers and a fourth diaphragm with a second preset number of layers, wherein the fourth diaphragm is connected with one gain module of the at least one gain module; wherein,
the reference optical fiber is used for transmitting monochromatic light matched with the type of the test task;
the third diaphragm is used for receiving monochromatic light transmitted by the reference optical fiber;
and the fourth diaphragm is used for receiving the monochromatic light transmitted by the third diaphragm.
6. An absorbance detection method capable of implementing the system according to any one of claims 1 to 5, wherein the method comprises:
the microprocessor selects an optical filter in the optical filter chip selection module according to a preset test task type, so that the optical filter filters the composite light emitted by the light source into monochromatic light matched with the test task type;
the microprocessor selects a test channel corresponding to the spectrum acquisition task matched with the type of the test task according to the spectrum acquisition task corresponding to each test channel in at least one preset test channel, and acquires a first light intensity value of monochromatic light transmitted through the test channel through an acquisition module;
the microprocessor selects a reference channel corresponding to the spectrum acquisition task matched with the type of the test task according to the spectrum acquisition task corresponding to each preset reference channel in at least one reference channel, and acquires a second light intensity value of the monochromatic light transmitted through the reference channel through the acquisition module;
and the microprocessor calculates the absorbance of the test substance in the test channel by using the first light intensity value and the second light intensity value.
7. The method of claim 6, wherein the microprocessor presets a spectrum acquisition task for each of the at least one test channel, comprising:
and setting each test channel in the at least one test channel as a spectrum acquisition task corresponding to the test channel, and setting the amplification factor of a gain chip selection module connected with the test channel as the amplification factor matched with the spectrum acquisition task.
8. The method of claim 7, wherein the microprocessor presets a spectrum acquisition task for each of the at least one reference channel, comprising:
setting each reference channel of the at least one reference channel as a spectrum acquisition task corresponding to the reference channel; and the respective amplification factor of the at least one gain module is matched with the amplification factor corresponding to the spectrum acquisition task.
9. The method of claim 7, wherein the at least one test channel comprises: the device comprises an incident optical fiber and a test position for containing test substances, wherein a first diaphragm with a first preset layer number is arranged on a light incident surface of the test position, a second diaphragm with a second preset layer number is arranged on a light emergent surface of the test position, and the second diaphragm is connected with one gain chip selection module in at least one gain chip selection module;
monochromatic light matched with the test task type is transmitted through the incident optical fiber, enters the test substance in the test position through the first diaphragm and the light incident surface of the test position, transmits the test substance and is transmitted to the second diaphragm through the light emergent surface of the test position.
10. The method of claim 9, wherein each of the at least one reference channel comprises: the reference optical fiber, a third diaphragm with a first preset number of layers and a fourth diaphragm with a second preset number of layers, wherein the fourth diaphragm is connected with one gain module of the at least one gain module; wherein,
and the monochromatic light matched with the type of the test task is transmitted through the reference optical fiber and is transmitted to the second diaphragm through the first diaphragm.
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