CN105987899A - Flow injection illuminating detection device - Google Patents

Abstract

The invention discloses a flow injection luminescent detection device, which comprises a base, a reference electrode, an auxiliary electrode and a working electrode. A detection cavity with an opening at the bottom is arranged inside the base, and the reference electrode and the auxiliary electrode are arranged along the base. Axially arranged in the base, one end of the reference electrode and the auxiliary electrode is suspended in the detection cavity, the other end of the reference electrode and the auxiliary electrode protrudes from the upper end surface of the base, and the working electrode is formed by Made of transparent material, the working electrode is fixed on the bottom wall of the base and is in contact with the detection chamber, the working electrode seals the bottom of the detection chamber, and two through holes are opened radially on the peripheral wall of the base, the The through hole communicates with the detection chamber. The invention solves the problem of constructing the bionano sensing interface in the flow injection chemiluminescence/electrochemiluminescence system, and realizes the highly selective and sensitive detection of biomolecules in the automatic flow injection chemiluminescence/electrochemiluminescence system.

Description

A flow injection luminescence detection device

technical field

The invention relates to the field of biochemistry, in particular to a flow injection luminescent detection device.

Background technique

In the prior art, when the biological nano-sensing interface is constructed in the chemiluminescence/electrochemiluminescence system, when the nanomaterials or biomolecules are detected after waiting for the detection substance to be fixed, the detection is performed separately according to the needs of different detection reagents, resulting in repeated operations. Many, affect the efficiency of the experiment. Due to the similarity of the detection process, whether flow injection chemiluminescence/electrochemiluminescence detection can be realized has been the direction of exploration in this field.

Contents of the invention

In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a flow injection luminescence detection device, which solves the problem of constructing the bio-nano sensing interface in the flow injection chemiluminescence/electrochemiluminescence system, and realizes automatic flow injection chemiluminescence /Highly selective and sensitive detection of biomolecules in electrochemiluminescent systems.

In order to solve the above problems, the technical scheme adopted in the present invention is as follows:

A flow injection luminescence detection device, comprising a base, a reference electrode, an auxiliary electrode and a working electrode, the base is provided with a detection cavity with an opening at the bottom, and the reference electrode and the auxiliary electrode are arranged on the base along the axial direction of the base In the base, one end of the reference electrode and the auxiliary electrode is suspended in the detection chamber, and the other end of the reference electrode and the auxiliary electrode protrudes from the upper end surface of the base, and the working electrode is made of transparent material , the working electrode is fixed on the bottom wall of the base and is in contact with the detection cavity. The working electrode seals the bottom of the detection cavity. cavities connected.

Preferably, it also includes a connection base, the reference electrode and the auxiliary electrode are arranged in the connection base along the axial direction of the connection base, the base is a hollow tubular body, and the connection base is sleeved in the hollow cavity on the upper part of the base In the body, the cavity between the base and the connecting seat is a detection cavity.

Preferably, the reference electrode is fixed on the connection base through a socket.

Preferably, the connecting seat is screwed to the base.

Preferably, a fixing plate is further included, the fixing plate is arranged on the lower side of the working electrode, and the working electrode abuts against the bottom wall of the base through the fixing plate.

Preferably, detection holes are opened on the fixing plate, and the fixing plate is fixed on the base by fixing pins.

Preferably, a seal is provided between the working electrode and the bottom wall of the base to ensure airtightness between the base and the working electrode.

Preferably, the reference electrode is an Ag/AgCl electrode or a saturated calomel electrode.

Preferably, the auxiliary electrode is a platinum electrode.

Preferably, the working electrode is a glass plate bonded with an ITO thin film.

Compared with the prior art, the beneficial effect of the present invention is that: the present invention sets a detection cavity in the base, and the reference electrode, the auxiliary electrode and the working electrode are respectively in contact with the detection cavity, and open a hole on the peripheral wall of the base to communicate with the detection cavity. The two through holes realize the continuous injection of the solution in the detection chamber and the real-time detection of the chemiluminescence/electrochemiluminescence of the substance to be detected on the working electrode, which solves the problem of bioluminescence in the flow injection chemiluminescence/electrochemiluminescence system. The construction of the nano-sensing interface realizes the highly selective and sensitive detection of biomolecules in the automated flow injection chemiluminescence/electrochemiluminescence system.

Description of drawings

Fig. 1 is a schematic structural view of a flow injection luminescent detection device in the present invention;

Fig. 2 is an exploded view of the flow injection luminescent detection device in Fig. 1;

Fig. 3 is a cross-sectional view of the flow injection luminescent detection device along line A-A in Fig. 1;

Fig. 4 is the structural representation of base in the present invention;

Fig. 5 is a sectional view of the base along the line B-B in Fig. 4;

Among them, 1 is the base, 11 is the through hole, 12 is the detection cavity, 2 is the connecting seat, 21 is the fixing hole, 3 is the reference electrode, 31 is the sleeve, 4 is the auxiliary electrode, 5 is the working electrode, 51 Is a seal, 6 is a fixed plate, 61 is a fixed pin, and 62 is a detection hole.

detailed description

The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

As shown in Figures 1, 3, 4, and 5, the flow injection luminescent detection device in the present invention includes a base 1, a reference electrode 3, an auxiliary electrode 4 and a working electrode 5, and a detection chamber with a bottom opening is arranged in the base 1 12. The reference electrode 3 and the auxiliary electrode 4 are arranged in the base 1 along the axial direction of the base 1, and one end of the reference electrode 3 and the auxiliary electrode 4 is suspended in the detection chamber 12 to conduct electricity to the solution in the detection chamber 12; The other ends of the reference electrode 3 and the auxiliary electrode 4 protrude from the upper surface of the base 1 to facilitate connection with an external power source. The working electrode 5 is made of a transparent material, and nanomaterials and biomolecules can be fixed on the working electrode to be detected. The working electrode 5 is fixed on the bottom wall of the base 1 and is in contact with the detection chamber 12. The bottom is sealed, and two through holes 11 are radially opened on the peripheral wall of the base 1 , and the through holes 11 communicate with the detection cavity 12 .

When in use, the flow injection luminescence detection device is placed above the photomultiplier tube (the device and the photomultiplier tube are both in the dark box, protected from light), and the main and auxiliary peristaltic pumps of the flow injection system pump the solution through one of the through holes 11. The detection chamber 12 can perform chemiluminescence or electrochemiluminescence detection: 1) When used for chemiluminescence measurement, the pumped solution reacts with the substance to be detected fixed on the working electrode 5, and the emitted chemiluminescence passes through the working electrode 5 The photomultiplier tube below detects that the waste liquid flows out from another through hole 11; 2) when it is used for electrochemiluminescence detection, the reference electrode 3, the auxiliary electrode 4 and the working electrode 5 are controlled by an electrochemical workstation, and the pumped solution An electrochemical reaction occurs on the working electrode 5 , and the emitted light is detected by a photomultiplier tube under the working electrode 5 . The detection chamber 12 provides a closed space for the reaction. The substance to be detected is fixed on the surface of the working electrode 5, and the reaction reagent is continuously introduced into the detection chamber 12 through the through hole 11 to realize rapid detection; solve the problem of flow injection chemiluminescence/electrochemiluminescence The construction of the biological nano-sensing interface in the system has realized the high selection and sensitive detection of biomolecules in the automated flow injection chemiluminescence/electrochemiluminescence system.

In this embodiment, the flow injection luminescence detection device also includes a connecting seat 2, the reference electrode 3 and the auxiliary electrode 4 are arranged in the connecting seat 2 along the axial direction of the connecting seat 2, the base 1 is a hollow tubular body, and there are two sets of connecting seats Connected to the hollow cavity on the upper part of the base 1 , the cavity between the base 1 and the connection seat 2 is a detection cavity 12 . The split design of the connection seat 2 and the base 1 facilitates cleaning of the detection cavity 12 inside the flow injection luminescence detection device.

The connecting seat 2 is screwed to the base 1, and the screwed connection is convenient for disassembly and can also provide better airtightness.

It can be understood that a sealing ring can also be directly sleeved on the connecting seat 2 , and the connecting seat 2 and the base 1 are interference-fitted by means of screw connection, so as to further ensure the airtightness between the connecting seat 2 and the base 1 .

Referring to Fig. 2, a fixing hole 21 is opened on the connection base 2, and the reference electrode 3 is fixed on the connection base 2 through a socket 31, the reference electrode 3 is a saturated calomel electrode, the auxiliary electrode 4 is a platinum electrode, and the working electrode 5 is It is a glass plate bonded with an ITO film. The sleeve 31 is provided with a fixing screw and a sealing rubber ring, which facilitates the replacement of the reference electrode 3 and ensures the airtightness between the reference electrode 3 and the connection seat 2 after replacement.

It can be understood that the reference electrode 3 can also be an Ag/AgCl electrode, and the working electrode 5 can also be other transparent substrate bonded with an ITO film, such as a transparent plastic plate.

In this embodiment, the flow injection luminescence detection device further includes a fixing plate 6 disposed on the lower side of the working electrode 5 , and the working electrode 5 abuts against the bottom wall of the base 1 through the fixing plate 6 . A detection hole 62 is opened on the fixing plate 6 , and the fixing plate 6 is fixed on the base 1 by a fixing pin 61 . The fixing plate 6 fixed by the fixing pin 61 facilitates the disassembly and assembly of the working electrode 5, and the working electrode 5 can be replaced by express delivery according to different experimental requirements. The detection hole 62 will not affect the detection process, ensuring the accuracy of the experiment.

It can be understood that when the fixed plate 6 is made of a transparent substrate, the detection hole 62 does not need to be opened.

Further, a seal 51 is provided between the working electrode 5 and the bottom wall of the base 1 , the seal 51 ensures airtightness between the base 1 and the working electrode 5 and ensures that the solution in the detection chamber 12 will not leak.

During implementation, the flow injection luminescence detection device is placed above the photomultiplier tube (the device and the photomultiplier tube are both in the dark box, protected from light), and the main and auxiliary peristaltic pumps of the flow injection system pump the solution through one of the through holes 11. The detection chamber 12 can perform chemiluminescence or electrochemiluminescence detection:

1) When used for chemiluminescence measurement, the working electrode 5 is immobilized with bioactive substances, and the pumped solution interacts with the bioactive substances immobilized on the working electrode 5, and the emitted chemiluminescence passes through the photoelectric multiplication under the fixed plate 6 tube detection, the waste liquid flows out from another through hole 11;

2) When used for electrochemiluminescence detection, the reference electrode 3, the auxiliary electrode 4 and the working electrode 5 are controlled by an electrochemical workstation, and the pumped solution undergoes an electrochemical reaction on the working electrode 5, and the emitted light passes through the fixed Plate 6 below the photomultiplier tube detection.

Those skilled in the art can make various other corresponding changes and deformations according to the above-described technical solutions and concepts, and all these changes and deformations should fall within the protection scope of the claims of the present invention.

Claims (10)

1. a flow injection luminescence detection apparatus, it is characterised in that include pedestal, reference electrode, auxiliary Electrode and working electrode, be provided with the test chamber of bottom opening, described reference electrode and auxiliary in described pedestal Electrode is axially disposed within pedestal along pedestal, and one end of described reference electrode and auxiliary electrode is suspended at test chamber In, the other end of described reference electrode and auxiliary electrode protrudes and is arranged on pedestal upper surface, described working electrode Being prepared by transparent material, described working electrode is fixed on the diapire of pedestal and contacts with test chamber, described work Making electrode by the sealed bottom of test chamber, described base peripheral wall radially offers two through holes, described through hole with Test chamber connects.

2. flow injection luminescence detection apparatus as claimed in claim 1, it is characterised in that also include connecting Seat, described reference electrode and auxiliary electrode are axially disposed within connection seat along connecting seat, and described pedestal is hollow Tubular body, described connection cover for seat is connected in the hollow cavity on pedestal top, described pedestal be connected between seat Cavity be test chamber.

3. flow injection luminescence detection apparatus as claimed in claim 2, it is characterised in that described reference electricity Pole is fixed on connection seat by Coupling.

4. flow injection luminescence detection apparatus as claimed in claim 2, it is characterised in that described connection seat It is spirally connected with pedestal.

5. flow injection luminescence detection apparatus as claimed in claim 1, it is characterised in that also include fixing Plate, described fixed plate is arranged on the downside of working electrode, and described working electrode is connected to pedestal by fixed plate On diapire.

6. flow injection luminescence detection apparatus as claimed in claim 5, it is characterised in that described fixed plate On offer detection hole, described fixed plate is fixed on pedestal by steady pin.

7. flow injection luminescence detection apparatus as claimed in claim 1, it is characterised in that described work electricity It is additionally provided with bubble-tight seal between guarantee pedestal and working electrode between pole and the diapire of pedestal.

8. the flow injection luminescence detection apparatus as described in any one of claim 1-7, it is characterised in that institute Stating reference electrode is Ag/AgCl electrode or saturated calomel electrode.

9. the flow injection luminescence detection apparatus as described in any one of claim 1-7, it is characterised in that institute Stating auxiliary electrode is platinum electrode.

10. the flow injection luminescence detection apparatus as described in any one of claim 1-7, it is characterised in that Described working electrode is the glass plate being fitted with ito thin film.