CN105717036B - A kind of flow cytometer flow chamber of magnetic stirring - Google Patents

Abstract

The present invention relates to the flow cytometer flow chamber of a kind of flow chamber of flow cytometer, more particularly to a kind of magnetic stirring.Flow chamber and sheath liquid chamber are passed through including flow chamber, sample introduction needle, sheath liquid chamber composition, the sample introduction needle, the sheath liquid chamber is located above flow chamber, is connected by sheath fluid apertures；Magnetic bead is equipped with the flow chamber, electromagnet is equipped between the flow chamber and sheath liquid chamber, the sheath liquid chamber upper end has sheath fluid entrance.Can stably stratified flow quickly, save the energy.When spout sprays, sheath fluid still carries centripetal force, and sample introduction needle is located at circle centre position and typhoon eye position, can ensure that its straight line drops, will not be mixed with sheath fluid, will not block spout.

Description

A magnetically stirred flow cytometer flow chamber

technical field

The utility model relates to a flow chamber of a flow cytometer, in particular to a flow chamber of a magnetic stirring type flow cytometer.

Background technique

Flow cytometer is a very common detection instrument. The flow chamber in its liquid circuit system is composed of sample tube, sheath liquid tube and nozzle. It is usually made of transparent and stable materials such as optical glass and quartz. The design and production are very fine, it is the heart of the fluid flow system. The sample tube stores the sample, and a single cell suspension is ejected from the sample tube under the action of the liquid flow pressure; the sheath liquid flows from the sheath liquid tube from the surrounding to the nozzle hole, surrounds the sample and then ejects from the nozzle to ensure a stable liquid flow. Due to the effect of the sheath fluid, the detected cells are confined to the axis of the fluid flow. Typically, the sample is injected into the center of the sheath fluid at a pressure higher than that of the sheath fluid. The sample flow rate is adjusted by adjusting the sample/sheath flow pressure ratio through a pressure regulator. The flow rate increases with increasing sample pressure, while the center of the sample is wider and more cells can pass through the laser detection point at a time. Conversely, lowering the sample pressure slows down the liquid flow and reduces the number of cells that pass through the laser detection point at a time. The flow chamber uses the principle of hydrodynamic focusing to converge the sheath flow and the sample flow in the flow chamber, so that the sample flow forms a stable laminar flow. At this time, the sheath flow surrounds the sample flow, and the sample flow forms a stable linear flow. The liquid passes through the laser The detection area is used for optical system detection and analysis, but the flow of the sheath fluid is unstable, especially when the detection speed is changed, the pressure changes and the flow rate is unstable, and the speed of the sheath fluid is generally only adjusted in the flow chamber, while the sheath fluid chamber It is the transition stage of the sheath fluid, which is used as the reserve of the sheath fluid in the flow chamber. However, when the sheath fluid enters the flow chamber, it often enters directly from the top of the flow chamber, which is very easy to cause fluid fluctuations, and it is difficult to form a stable laminar flow, which affects the experimental results.

CN201520743256.1 discloses a liquid inlet assembly device for a flow cytometer, which includes three structures of a trapezoidal cavity sleeve, a sheath flow chamber and a sampling needle; the trapezoidal cavity sleeve is provided with four Sheath fluid hole, injection needle hole one, trapezoidal cavity and cylindrical cavity. The sheath flow chamber includes two injection needle holes, four annular slots and a flange-shaped boss. The liquid inlet assembly adopts a trapezoidal cavity sleeve design, which has a larger inlet area, so as to achieve a large flow of sheath liquid without changing the radius of the sheath flow chamber. The liquid inlet of the sheath flow chamber is designed with four annular slots, so as to increase the flow rate of the sheath liquid, ensure that the sheath liquid flows evenly, and avoid turbulent flow. The assembly device utilizes inexpensive and readily available plastic fittings, and the liquid inlet assembly device has the characteristics of simple structure and easy assembly and disassembly by users.

CN201520703127.X relates to a novel flow cytometer flow chamber, which mainly includes a flow chamber, a sampling needle, an upper end cover, a sleeve, a lower end cover, a rotating piece, two sheath inlets are arranged on the top of the upper end cover, and the rotating piece Two annular grooves of the same size and shape are opened on the top of the sleeve, and a first sheath flow chamber, a first focus area, a second sheath flow chamber, and a second focus area are arranged in the flow chamber. The utility model can form a stable laminar flow, avoid turbulent flow, and maintain high detection accuracy when the sampling rate is different.

But they didn't solve the sheath fluid chamber. The sheath fluid starts to have velocity in the flow chamber, especially when the machine is turned on, it takes a long time to form a stable laminar flow. It is easy to clog the jet or the sample contaminates the flow cell during continuous injection.

Utility model content

The problem to be solved: the utility model uses magnetic rotation to make the sheath liquid in the sheath chamber have an initial velocity, and uses magnetic stirring in the flow chamber to make the laminar flow stable, even if the speed is changed, it will not be affected. Especially when the machine is just turned on, the laminar flow is quickly stabilized and energy is saved. The sheath liquid still has centripetal force when it is ejected from the nozzle, and the injection needle is located at the center of the circle and the position of the typhoon eye, which can ensure that it falls in a straight line without mixing with the sheath liquid or blocking the nozzle.

Technical solutions:

A flow cytometer flow chamber of a magnetic stirring type, comprising a flow chamber, a sampling needle, and a sheath fluid chamber, the sampling needle passes through the flow chamber and the sheath fluid chamber, and the sheath fluid chamber is located in the flow chamber The upper part is connected through the sheath liquid hole; magnetic beads are arranged in the flow chamber, and an electromagnet is arranged between the flow chamber and the sheath liquid chamber.

Furthermore, the flow chambers are divided into flow chamber one, flow chamber two, and flow chamber three from top to bottom, and there is a transition section between the flow chambers, which is wide at the top and narrow at the bottom.

Furthermore, the first flow chamber, the second flow chamber, and the third flow chamber are all cylinders, and the cross-sectional area of the first chamber is smaller than that of the second chamber, and the cross-sectional area of the second chamber is smaller than that of the third chamber.

Furthermore, the magnetic beads are inverted magnetic beads located on the top of the flow chamber, and the magnetic beads are movably connected with the flow chamber.

Furthermore, magnetic beads of the sheath fluid chamber are arranged inside the sheath fluid chamber, and the magnetic beads of the sheath fluid chamber are located in the center of the sheath fluid chamber.

Furthermore, the magnetic beads have a through hole in the middle.

Further, the electromagnet is divided into a sheath liquid chamber electromagnet and a flow chamber electromagnet.

Further, there is an insulating layer between the electromagnet of the sheath liquid chamber and the electromagnet of the flow chamber.

Further, the sheath fluid hole takes the sampling needle as the center of a circle, and there is one hole every 60°, forming no less than two concentric circles.

Furthermore, a protective cover is provided outside the injection needle.

Beneficial effect:

The utility model creatively adopts the method of magnetic stirring in the flow cytometer for the first time to control the flow direction and velocity of the sheath fluid, which breaks through the previous control mode of the sheath fluid. Magnetic rotation is used to make the sheath liquid in the sheath liquid chamber have an initial velocity, and magnetic stirring is used in the flow chamber to make the laminar flow stable, even if the speed is changed, it will not be affected. Especially when the machine is just turned on, the laminar flow is quickly stabilized and energy is saved. When the nozzle is ejected, the sheath liquid still has centripetal force. The injection needle is located at the center of the circle and the position of the typhoon eye, which can ensure that it falls in a straight line without mixing with the sheath liquid or blocking the nozzle.

1 The cylinder design is convenient for installation and molding, and has a large volume, smooth surroundings, no obstacles, and a stable flow rate of the sheath fluid in the chamber. It does not cause fluctuations in the flow cell, thereby ensuring a stable sample flow. The decreasing cross-sectional area can control the flow rate of the sheath liquid by changing the size of the flow chamber, thereby ensuring the requirement of different sample flow injection rates while maintaining high detection accuracy.

2. The inverted magnetic beads are located on the top of the flow chamber, and are connected to the top for easy disassembly and replacement, and occupy a small space, because the top of the flow chamber is the widest, and it is located at the top to save space. When the sheath fluid enters the flow chamber, its speed is adjusted. The sheath fluid needs to form a certain flow direction, increase its power, and minimize the fluctuation of laminar flow.

3. Magnetic beads of the sheath fluid chamber are arranged inside the sheath fluid chamber, and the magnetic beads of the sheath fluid chamber are located in the center of the sheath fluid chamber. The magnetic bead stirring in the sheath liquid chamber makes the sheath liquid have an initial velocity in the sheath liquid chamber, increases the kinetic energy of the sheath liquid, reduces the speed difference to the sheath liquid in the flow chamber, and achieves the effect of stable laminar flow. Centrally located to equalize velocity throughout the sheath fluid chamber.

The magnetic bead described in 4 has a hole in the middle, which can rotate around the injection needle, does not take up space and does not need to change the direction of the injection needle, and reduces the difficulty of casting. Does not affect the overall structure.

5. The electromagnet is divided into a sheath fluid chamber electromagnet and a flow chamber electromagnet, respectively controlling the rotational speed of the sheath fluid chamber magnetic beads and the flow chamber magnetic beads. Generally, the speed of the sheath liquid chamber is lower than the speed of the magnetic beads in the flow chamber to prevent the high speed of the sheath liquid chamber from flowing into the flow chamber due to the inertia of the liquid. After the two-stage magnetic stirring is accelerated, the design of the gradient flow chamber achieves the purpose of continuing to accelerate and form laminar flow, which saves energy and is convenient to control.

6 There is an insulating layer between the electromagnet of the sheath liquid chamber and the electromagnet of the flow chamber to prevent the magnetization of the instrument components from mutual influence and reduce the accuracy of the instrument, which can prolong the service life of the instrument.

7. The sheath fluid hole allows the sheath fluid to flow into the flow chamber under the action of pressure and gravity, and plays the role of supplementary sheath fluid. It is different from the previous method of flow cytometer with two holes or a few holes to allow it to flow in. Here, multiple The hole enters, the speed is increased quickly, and the dispersed entry reduces the influence on the laminar flow of the flow chamber.

8 There is a protective cover on the outside of the sampling needle to prevent electromagnetic interference and magnetization of the sampling needle, so that the sample to be tested is in good condition and will not affect the accuracy of the detection data.

Description of drawings:

Fig. 1 is a kind of magnetic stirring type flow cytometer flow chamber structure schematic diagram;

Fig. 2 is a kind of flow cytometer flow chamber magnetic bead structure schematic diagram of magnetic stirring type;

1-injection needle; 2-protective cover; 3-sheath liquid chamber; 4-sheath liquid hole; 5-flow chamber 1; 6-transition section; 7-flow chamber 2, 8-flow chamber 3; 9-nozzle; 10 - inverted magnetic beads; 11 - electromagnet; 12 - magnetic beads in sheath liquid chamber; 13 - sheath liquid inlet; 14 - through hole; 15 - insulating layer.

Detailed ways

Example 1

As shown in Figure 1 and Figure 2, a flow cytometer flow chamber of a magnetic stirring type comprises a flow chamber, a sampling needle 1, and a sheath fluid chamber 3, and the sampling needle 1 passes through the flow chamber and the sheath fluid Chamber 3, the sheath fluid chamber 3 is located above the flow chamber and connected through the sheath fluid hole 4; magnetic beads are arranged in the flow chamber, and an electromagnet 11 is arranged between the flow chamber and the sheath fluid chamber 4.

Furthermore, the flow chamber is divided into flow chamber 5, flow chamber 2, and flow chamber 8 from top to bottom, and an upper width Lower narrow transition section 6 .

Further, the first flow chamber 5 , the second flow chamber 7 , and the third flow chamber 8 are all cylinders, and the cross-sectional area of the first chamber 5 is smaller than that of the second chamber 7 ; the cross-sectional area of the second chamber 7 is smaller than that of the third chamber 8 .

Furthermore, the magnetic beads are inverted magnetic beads 10 located on the top of the flow chamber, and the magnetic beads are movably connected with the flow chamber.

Furthermore, the sheath fluid chamber 3 is provided with magnetic beads 12 inside the sheath fluid chamber, and the sheath fluid chamber magnetic beads 12 are located in the center of the sheath fluid chamber 3 .

Furthermore, the said magnetic bead has a through hole 14 in the middle.

Furthermore, the electromagnet 11 is divided into a sheath fluid chamber electromagnet and a flow chamber electromagnet.

Further, there is an insulating layer 15 between the electromagnet of the sheath liquid chamber and the electromagnet of the flow chamber.

Furthermore, the sheath fluid hole 4 takes the sampling needle 1 as the center of the circle, and there is one sheath fluid hole 4 every 60°, forming no less than two concentric circles.

Furthermore, a protective cover 2 is provided outside the sampling needle 1 .

As mentioned above, the upper part of the sheath fluid chamber has a sheath fluid inlet 13 .

Claims (10)

1. A flow cytometer flow chamber of magnetic stirring type, is characterized in that, comprises flow chamber, sampling needle, sheath fluid chamber is formed, and described sampling needle passes flow chamber and sheath fluid chamber, and described The sheath fluid chamber is located above the flow chamber and is connected through a sheath fluid hole; magnetic beads are arranged in the flow chamber, an electromagnet is arranged between the flow chamber and the sheath fluid chamber, and a sheath fluid chamber is provided at the upper end of the sheath fluid chamber. Entrance. 2. the flow cytometer flow chamber of a kind of magnetic stirring type according to claim 1, it is characterized in that, described flow chamber is divided into flow one chamber, flow two chambers, flow three chambers respectively from top to bottom, so A transition section that is wide at the top and narrow at the bottom is provided between the above-mentioned flow chambers. 3. a kind of flow cytometer flow chamber of magnetic stirring type according to claim 2, it is characterized in that, described flow one chamber, flow two chambers, flow three chambers are cylinders, and one chamber cross-sectional area Less than the second chamber, and the cross-sectional area of the second chamber is smaller than that of the third chamber. 4. The flow cell of a magnetic stirring type flow cytometer according to claim 1, wherein the magnetic beads are inverted magnetic beads located at the top of the flow cell, and the magnetic beads are movable with the flow cell connect. 5. The flow cell of a magnetic stirring type flow cytometer according to claim 1, wherein the magnetic beads of the sheath liquid chamber are arranged inside the sheath liquid chamber, and the magnetic beads of the sheath liquid chamber are located at The center of the sheath fluid chamber. 6. The magnetically stirred flow cytometer flow chamber according to claim 1, wherein said magnetic beads have a through hole in the middle. 7. The flow cell of a magnetically stirred flow cytometer according to claim 1, wherein the electromagnet is divided into a sheath fluid chamber electromagnet and a flow chamber electromagnet. 8. The flow cell of a magnetically stirred flow cytometer according to claim 7, wherein an insulating layer is provided between the electromagnet of the sheath liquid chamber and the electromagnet of the flow chamber. 9. A magnetic stirring type flow cytometer flow chamber according to claim 1, characterized in that the sheath fluid hole takes the sampling needle as the center of a circle, and there is one hole every 60°, forming no less than two concentric circles . 10. A magnetic stirring type flow cytometer flow chamber according to claim 1, characterized in that a protective cover is provided outside the sampling needle.