CN103882089A - High-flux microorganism detecting method for qinglu tincture - Google Patents
High-flux microorganism detecting method for qinglu tincture Download PDFInfo
- Publication number
- CN103882089A CN103882089A CN201210560914.4A CN201210560914A CN103882089A CN 103882089 A CN103882089 A CN 103882089A CN 201210560914 A CN201210560914 A CN 201210560914A CN 103882089 A CN103882089 A CN 103882089A
- Authority
- CN
- China
- Prior art keywords
- liquid
- test
- escherichia coli
- bacterium
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of microorganisms. The objective of the invention is to provide a microorganism limit detecting method of a qinglu tincture so as to facilitate research in medical science and biology. By membrane filtration, microorganisms are fully intercepted by a filtration membrane, and are washed with a proper amount of interference-free washing liquid so as to remove interference of a sample to be detected, and therefore the detection of the microorganism limit can be accurately performed. The using amount of the washing liquid is critical. Excessive washing can impair bacteria and is not conductive to growth, thus influencing the recovery rate. In addition, excessive washing will damage the filtration membrane so that the pore diameter is changed and the experiment results are influenced. Otherwise, insufficient washing cannot completely eliminate the antibacterial function of the sample, so that the accuracy of the experiment results cannot be guaranteed.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method of clear dew tincture limit test of microbe, contribute to the research to microorganism from now on of medical science and biology aspect.
Background technology
Clear dew tincture has more reasonably used the traditional Chinese medical science to take stopgap measures and has consolidated, and enriching yin wets one's whistle, clearing heat and detoxicating diagnosis and treatment based on an overall analysis of the illness and the patient's condition.Balloonflower root in prescription, Radix Glycyrrhizae clearing throat medicine carrying are up, join the Fructus Aurantii lifting mechanism of qi of regulating the flow of vital energy.The tuber of dwarf lilyturf, asparagus fern, the stem of noble dendrobium, the dried rhizome of rehmannia, cultivated land the is nourishing Yin and clearing heat anti-inflammatory that wets one's whistle.Full side reaches enriching yin and wets one's whistle, clearing heat and detoxicating (relieve sore throat).Treating acute and chronic pharyngitis, laryngeal edema.This product smell side perfume (or spice) is pure, good mouthfeel, and suitable patient takes, and has no untoward reaction, is clinical treatment acute and chronic pharyngitis, laryngeal edema good medicine.
Limit test of microbe genealogy of law test procedure specifies that sterilization preparation and raw material thereof, auxiliary material are subject to the method for microbial contamination degree, is also important means and the foundation with raw material, auxiliary material, equipment, utensil, technical process, environment and operator's sanitary condition of evaluating manufacturing enterprise.Inspection item comprises microbiological contamination amount and Control bacteria examination.
Summary of the invention
The clear tincture that reveals is made up of Chinese medicines such as balloonflower root, the stem of noble dendrobium, the dried immature fruit of citron orange, the dried rhizome of rehmannia, Radix Glycyrrhizae, cultivated land, the tuber of dwarf lilyturf and asparagus ferns, has treatment pharyngalgia, coughs and breathe heavily and heat-clearing toxin-expelling functions, occurs a small amount of precipitation after placing, on quality effect without impact.The clear tincture that reveals contains 15% ethanol, and low alcohol environment has interference to microorganism growth, can affect the accuracy of test-results.Test shows, measuring according to a conventional method its rate of recovery is only 50% ~ 60%, undesirable (should be more than 70%).By adopting membrane-filter procedure to measure, can reduce the impact of ethanol on test-results.
Embodiment
1, the supernatant liquor that test liquid is prepared sample thief adds diluent and makes test liquid.
2, bacterium solution preparation is prepared escherichia coli, streptococcus aureus, subtilis, Candida albicans and black-koji mould bacterium liquid by two methods of Chinese Pharmacopoeia version in 2005, and another compound concentration is 10 ~ 100cfu/ml escherichia coli liquid.
3, get test liquid and add in filter through membrane filtration post-flush, in washing fluid the last time, add escherichia coli, streptococcus aureus, subtilis bacterium liquid or Candida albicans and black-koji mould bacterium liquid, filter.Take out filter membrane, bacterium faces up and is affixed on nutrient agar or Rose Bengal Sodium nutrient agar, cultivates.2 plates of the parallel preparation of every strain test organisms, counting.
embodiment 1
1, test liquid is prepared supernatant liquor 10 ml of sample thief, adds diluent to 100ml, makes 1 10 test liquids.
2, bacterium solution preparation escherichia coli, streptococcus aureus, subtilis, Candida albicans and black-koji mould bacterium liquid are all by two method preparations of Chinese Pharmacopoeia version in 2005, concentration is 50 ~ 100cfu/ml, and another compound concentration is 10 ~ 100cfu/m l escherichia coli liquid.
3, getting 1 10 test liquid 1ml adds in the filter that 50ml diluent is housed, after membrane filtration, rinse 3 times, each 100ml, in washing fluid the last time, respectively add 50 ~ 100cfu/ml escherichia coli, streptococcus aureus, subtilis bacterium liquid or Candida albicans and the black-koji mould bacterium liquid of 1ml, filter.Take out filter membrane, bacterium faces up and is affixed on nutrient agar or Rose Bengal Sodium nutrient agar, puts respectively 30 ~ 35 DEG C and cultivates 48 h or put 23 ~ 28 DEG C and cultivate 72h.2 plates of the parallel preparation of every strain test organisms.Press Chinese Pharmacopoeia version method for counting colonies counting in 2005.
Claims (4)
1. a clear method of revealing tincture limit test of microbe, is characterized in that: realize by following steps: the supernatant liquor that (1) test liquid is prepared sample thief adds diluent and makes test liquid; (2) bacterium solution preparation is prepared escherichia coli, streptococcus aureus, subtilis, Candida albicans and black-koji mould bacterium liquid by two methods of Chinese Pharmacopoeia version in 2005, and another compound concentration is 10 ~ 100cfu/ml escherichia coli liquid; (3) get test liquid and add in filter through membrane filtration post-flush, in washing fluid the last time, add escherichia coli, streptococcus aureus, subtilis bacterium liquid or Candida albicans and black-koji mould bacterium liquid, filter; Take out filter membrane, bacterium faces up and is affixed on nutrient agar or Rose Bengal Sodium nutrient agar, cultivates; 2 plates of the parallel preparation of every strain test organisms, counting.
2. cell as claimed in claim 1 breeding method altogether, is characterized in that: supernatant liquor 10 ml of step (1) sample thief, add diluent to 100ml, and make 1 10 test liquids.
3. cell as claimed in claim 1 breeding method altogether, it is characterized in that: step (2) escherichia coli, streptococcus aureus, subtilis, Candida albicans and black-koji mould bacterium liquid are all by two method preparations of Chinese Pharmacopoeia version in 2005, concentration is 50 ~ 100cfu/ml, and another compound concentration is 10 ~ 100cfu/m l escherichia coli liquid.
4. cell as claimed in claim 1 breeding method altogether, it is characterized in that: step (3) is got 1 10 test liquid 1ml and added in the filter that 50ml diluent is housed, after membrane filtration, rinse 3 times, each 100ml, in washing fluid the last time, respectively add 50 ~ 100cfu/ml escherichia coli, streptococcus aureus, subtilis bacterium liquid or Candida albicans and the black-koji mould bacterium liquid of 1ml, filter; Take out filter membrane, bacterium faces up and is affixed on nutrient agar or Rose Bengal Sodium nutrient agar, puts respectively 30 ~ 35 DEG C and cultivates 48 h or put 23 ~ 28 DEG C and cultivate 72h; 2 plates of the parallel preparation of every strain test organisms; Press Chinese Pharmacopoeia version method for counting colonies counting in 2005.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210560914.4A CN103882089A (en) | 2012-12-21 | 2012-12-21 | High-flux microorganism detecting method for qinglu tincture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210560914.4A CN103882089A (en) | 2012-12-21 | 2012-12-21 | High-flux microorganism detecting method for qinglu tincture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103882089A true CN103882089A (en) | 2014-06-25 |
Family
ID=50951210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210560914.4A Pending CN103882089A (en) | 2012-12-21 | 2012-12-21 | High-flux microorganism detecting method for qinglu tincture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103882089A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095968A (en) * | 2017-04-24 | 2017-08-29 | 许昌学院 | A kind of anti-haze effervescent tablet of relieving sore-throat clearing lung-heat and preparation method thereof |
-
2012
- 2012-12-21 CN CN201210560914.4A patent/CN103882089A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095968A (en) * | 2017-04-24 | 2017-08-29 | 许昌学院 | A kind of anti-haze effervescent tablet of relieving sore-throat clearing lung-heat and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102525857B (en) | Hand wash for disinfection and sterilization | |
| CN104651473B (en) | The method for determining the sub-lethal dose staphylococcus aureus resistance to the action of a drug and drug resistance simultaneously | |
| CN101933966B (en) | Active site composition of isatis roots, as well as preparation method and application thereof | |
| CN109452320A (en) | The purposes of Fructus Caryophylli volatile oil | |
| CN103007245B (en) | Medicament for sterilizing by personnel in clinical laboratory | |
| CN103882089A (en) | High-flux microorganism detecting method for qinglu tincture | |
| Yewale et al. | Benefits of Soleris® over the conventional method for enumeration of microbial load in Salacia herbal extract | |
| CN106309526B (en) | Composition of panax notoginseng saponins and fluconazole of candida albicans resistant strain and application thereof | |
| CN101698879A (en) | Medicament sensitivity testing method for detecting electrical impedance curve form of bacteria culture medium | |
| CN102250170A (en) | Preparation method and application of two active flavonoid glycosides in okra fruits | |
| CN103834718A (en) | Microbe limit inspection method of Qinglu tincture | |
| CN109125399A (en) | The antibacterial action of stephania tetrandra extract | |
| CN105661548B (en) | A kind of blueness cumquat ferment and preparation method thereof, purposes | |
| CN104306492A (en) | Drug with anti-bacterial and synergistically anti-bacterial activity, and preparation method and application thereof | |
| CN104304251B (en) | A kind of azoles is for the preparation of the purposes of disinfectant use in agriculture | |
| CN108398496A (en) | A kind of natural products Determination of Antibacterial Activity method | |
| CN102600276A (en) | Traditional Chinese medicine compound capable of eliminating drug resistance of escherichia coli as well as preparation method and application thereof | |
| CN101502537A (en) | Application of ginkgo episperm dry distillation liquid in preparing medicament for treating or preventing fungal disease | |
| CN103083448A (en) | Rhubarb and phellodendron medicinal pair extract for synergistically improving tinea pedis resistance | |
| CN104840701A (en) | Gel for treating vaginitis | |
| CN103041010B (en) | Compound cortex phellodendri chinensis preparation with tinea pedis treatment function | |
| Naldi et al. | Comparison of The Effectiveness of Bandotan Leaves (Ageratum conyzoides Linnaeus) and Sintrong Leaves (Crassocephalum crepidioides) Extracts on The Growth of The Bacteri Staphylococcus aureus ATCC 6538 | |
| CN108991006A (en) | A kind of graphene oxide compounding drip irrigation preventing and treating plant phytophthora root rot | |
| CN108812699A (en) | A kind of plant phytophthora root rot antibacterial agent of graphene oxide and Antagonistic Fungi compounding | |
| CN108812693A (en) | Application of the graphene oxide in terms of improving plant phytophthora germ Antagonistic Fungi preventive effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140625 |