CN103344643A - Semi-automatic external detection equipment and method for measuring specific egg white by turbidimetry - Google Patents
Semi-automatic external detection equipment and method for measuring specific egg white by turbidimetry Download PDFInfo
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- CN103344643A CN103344643A CN2013102818602A CN201310281860A CN103344643A CN 103344643 A CN103344643 A CN 103344643A CN 2013102818602 A CN2013102818602 A CN 2013102818602A CN 201310281860 A CN201310281860 A CN 201310281860A CN 103344643 A CN103344643 A CN 103344643A
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Abstract
The invention discloses semi-automatic external detection equipment and method for measuring specific egg white by turbidimetry. The method comprises the followings steps that: when a timer detects that the reaction time of a first detection reagent and a sample to be detected in a reaction cup is up, a microcontroller controls a sample adding needle to shift to the reaction cup and to be aligned to the center of the reaction cup; the microcontroller starts a loading pump to suck up a second detection reagent from a second detection reagent container, and the second detection reagent is added into the reaction cup for reaction; the content of the specific egg white in the sample to be detected is determined through turbidity of reactive materials in the reaction cup. The semi-automatic external detection equipment and method provided by the invention have the advantages that the automatic loading of the second detection reagent is realized, and the operation of manually loading the second detection reagent is omitted, so that the labor cost of the specific egg white detection is saved, and a condition that faults in an operation of manually and dropwise adding a detection reagent influence the detection result is also avoided as far as possible; meanwhile, the efficiency of the specific egg white detection is improved, the time of the specific egg white detection is greatly shorted, and thus the semi-automatic external detection equipment has wide market prospect.
Description
Technical field
The present invention relates to in-vitro diagnosis equipment, in particular a kind of semi-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein.
Background technology
So-called specific protein refers to have certain physiological function in the body, plays those specific proteins of important pathology sense when morbid state again.By these specific proteins being detected the physiological situation that to determine patient's body usually.Existing specific protein can be used for following clinical detection: immune dysfunction (detecting IgA, IgG, IgM, complement C3, complement C4, κ light chain, lambda light chain); Inflammatory conditions monitoring (detecting C-reactive protein, α acidoglycoprotein, haptoglobin, CER); The monitoring of rheumatism (detection type rheumatism factor R F, antistreptolysin O (ASO), C-reactive protein) cardiovascular function (detects super quick C-reactive protein, aPoA 1, apolipoprotein B, lipoprotein a); Renal function detects (detecting microdose urine protein, transferrins, α 1-microglobulin, β2Wei Qiudanbai, IgG); Nutrition condition monitoring (detecting albumin, prealbumin, transferrins); Blood disease (detecting antithrombin, transferrins, haptoglobin); The nervous system disease (detecting albumin, alpha2 Macroglobulin, IgA, IgG, IgM) etc.
At present, the common methods of measuring specific protein is turbidimetry, for example scattering turbidimetry method or turbidimetry, this method is that detected sample is added to earlier among the reagent R1, reaction a period of time adds reagent R2 again, after reacting a period of time again, the turbidity of measuring reaction product changes, and determines the content of specific protein in the detected sample.
Existing equipment based on turbidimetry detection specific protein has two kinds, a kind of is large automatic Biochemical Analyzer, usually be used in clinical laboratory or the centralab of big-and-middle-sized hospital, another kind of small-sized semi-automatic checkout equipment generally is used in clinical department or She Kang center.
Small-sized semi-automatic specific protein checkout equipment as shown in Figure 1 at present, monitor station 10 is provided with the Photoelectric Detection module 20 of a plurality of band temperature controls and agitating function, the testing staff is equipped with detected sample S in the reaction cup 21 that detects reagent R1 manual the adding in advance during measurement, again reaction cup 21 is inserted in the Photoelectric Detection module 20, two kinds of materials are reacted a period of time in reaction cup, and then will detect in the reagent R2 adding reaction cup 21, react a period of time again, measure the turbidity of reaction product in the reaction cup by the microcontroller of checkout equipment, thereby calculate the content of specific protein in the detected sample.Yet this detection mode exists following defective:
1) detecting reagent R2 needs to add manually, because reaction cup is the upper end tapered opening, the lower end is chamber, Vertical Narrow footpath, manually splash into when detecting reagent, very easily because testing staff's misoperation, part is detected on the inwall that reagent drops in reaction cup, make the quantity not sufficient of the detection reagent of participating in reaction, and then influence testing result;
2) reaction needed a period of time of detection reagent R1 and detected sample, the instrument alert adds when detecting reagent R2, because detecting the speed of reagent, each operating personnel's skilled operation degree or adding can have nothing in common with each other, therefore, it is different that this can make detection reagent and the reaction time of detected sample exist, and causes between the testing result also can there are differences; Simultaneously, the mode of this artificial interpolation has increased testing staff's workload, and is prone to monitoring error and influences testing result.
3) addition that detects reagent R2 generally is several microlitres, and operating personnel are had very high technical ability requirement, and careless slightly will causing detected the reagent dosage deficiency, as easy as rolling off a log generation error, thus influence the accuracy of measurement result.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
At the above-mentioned defective of prior art, the invention provides a kind of semi-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein, realize detecting the automatic loading of reagent, and then realize the robotization that specific protein detects.
The technical scheme that technical solution problem of the present invention adopts is as follows:
A kind of semi-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, comprise a monitor station, described monitor station is provided with the Photoelectric Detection module of band temperature control and agitating function, be placed with the reaction cup that detects reagent for prepackage first at described Photoelectric Detection module, on described monitor station, also be provided with a mechanical arm, load pump and the container that holds the second detection reagent, one sample needle is installed on the described mechanical arm, one end of described load pump connects described sample needle, the other end connects described container, also is provided with the microcontroller for the described checkout equipment testing process of control on monitor station.
Described employing turbidimetry is measured the semi-automatic vitro detection equipment of specific protein, and wherein, described Photoelectric Detection module includes several.
Described employing turbidimetry is measured the semi-automatic vitro detection equipment of specific protein, wherein, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
Described employing turbidimetry is measured the semi-automatic vitro detection equipment of specific protein, and wherein, described load pump is fixed displacement pump.
Described employing turbidimetry is measured the semi-automatic vitro detection equipment of specific protein, and wherein, described fixed displacement pump is ram pump or syringe pump.
Described employing turbidimetry is measured the semi-automatic vitro detection equipment of specific protein, wherein, described Photoelectric Detection module comprises a light source that is arranged on reaction cup one side, is arranged on the reaction cup opposite side and light source is the photoelectric sensor that predetermined angular arranges over against the optical filter that arranges and with light source.
Adopt turbidimetry to measure the semi-automatic external detection method of specific protein according to described checkout equipment, wherein, comprise step:
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, the microprocessor controls sample needle moves to the reaction cup place along mechanical arm, and aims at the reaction cup center;
Microcontroller start-up loading pump detects and to draw second the container of reagent and detect reagent from holding second, and it is joined in the reaction cup reacts;
Determine the content of specific protein in the detected sample by the turbidity of reactive material in the reaction cup.
Described employing turbidimetry is measured the semi-automatic external detection method of specific protein, wherein, the step of determining the content of specific protein in the detected sample by the turbidity of reactive material in the reaction cup also comprises afterwards: microcontroller is controlled sample needle again and is moved, sample needle is moved in the next reaction cup, and detect reagent with second and join in this reaction cup, carry out next group and detect.
Employing turbidimetry provided by the present invention is measured the semi-automatic vitro detection Apparatus and method for of specific protein, its technical advantages that reaches is: by the setting of mechanical arm, load pump and sample needle, realize detecting the automatic loading of reagent, save the cost of labor that specific protein detects, the operation error of having avoided artificial dropping to detect reagent impacts testing result; The present invention has simultaneously improved the detection efficiency of specific protein, has vast market prospect.
Description of drawings
Fig. 1 is the structural representation of specific protein checkout equipment in the prior art.
Fig. 2 is the structural representation that employing turbidimetry provided by the invention is measured the semi-automatic vitro detection equipment of specific protein.
Fig. 3 is the structural representation that employing turbidimetry provided by the invention is measured reaction cup in the semi-automatic vitro detection equipment of specific protein.
Fig. 4 is the structural representation that employing turbidimetry provided by the invention is measured Photoelectric Detection module in the semi-automatic vitro detection equipment of specific protein.
Fig. 5 is the process flow diagram that employing turbidimetry provided by the invention is measured the semi-automatic external detection method of specific protein.
Fig. 6 is the process flow diagram that employing turbidimetry provided by the invention is measured the another preferred embodiment of semi-automatic external detection method of specific protein.
Embodiment
For making purpose of the present invention, technical scheme and advantage clearer, clear and definite, below develop simultaneously with reference to accompanying drawing that the present invention is described in more detail for embodiment.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
The invention provides a kind of semi-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, as shown in Figure 2, comprise a monitor station 100, described monitor station 100 is provided with the Photoelectric Detection module 110 of several band temperature controls and agitating function, and on these Photoelectric Detection modules 110, be placed with reaction cup 200 respectively, reaction cup 200 is used for prepackage first and detects reagent and detect reagent as first, detected sample and second detects the reaction vessel of reagent, on described monitor station 100, also be provided with a mechanical arm 101, mechanical arm 101 is fixed on the monitor station 100 by screw 140, one sample needle 103 also is installed on mechanical arm 101, sample needle can move along mechanical arm 101, on monitor station, also be provided with a load pump 102, one end of this load pump 102 connects described sample needle 103, its other end is connected in the container 104 that is contained with the second detection reagent, this container 104 is arranged on the monitor station 100, load pump 102 is connected by conduit 120 with sample needle 103 and container 104, but is not limited to conduit.
Further, vitro detection equipment provided by the invention can detect for single channel, can be multi-channel detection, single channel only arranges a Photoelectric Detection module on monitor station when detecting, and during multi-channel detection, present embodiment includes several Photoelectric Detection modules, and this moment, the Photoelectric Detection module was arranged in a linear at monitor station, the quantity of Photoelectric Detection module is arranged as required, usually but be not limited to 4 to 8, the modes of emplacement of this straight-line has clearly advantage with respect to existing circular modes of emplacement.Reaction cup structural representation as shown in Figure 3, reaction cup 200 is generally the upper end pyramidal structure, opening is square, the lower end is vertical cavity, vertically cavity diameter is less, these reaction cup are placed on the Photoelectric Detection module, if adopt circular the placement, the placement orientation difference of each reaction cup then, splashing in the process that detects reagent or detected sample to one of them, be easy to cause detection reagent or detected sample to be dropped on the inwall of reaction cup, influencing testing result, and circular structure of placing is complicated, and cost is higher.Can reduce as much as possible and detect reagent or detected sample and dropped in probability on the reaction cup inwall, reduce the error risk and adopt straight-line that the Photoelectric Detection module is set.Simultaneously, straight-line arranges the control that the Photoelectric Detection module also can be simplified sample needle greatly.Certainly, the Photoelectric Detection module is not limited to adopt line spread at monitor station, also can adopt circular arc to arrange or other arrangement modes.
The arrangement mode of the structure of mechanical arm of the present invention and Photoelectric Detection module is adaptive, in the present embodiment, arrange because the Photoelectric Detection module is orthoscopic, therefore mechanical arm of the present invention is preferably linear structure, and this linear structure can be simplified the movement of sample needle on mechanical arm.Mechanical arm can be arranged on but be not limited to the Photoelectric Detection module directly over, directly over being preferably, like this, sample needle just can be aimed at reaction cup, is convenient to detect the interpolation of reagent.
In order to realize that well second detects the automatic loading of reagent, meeting of the present invention arranges a microcontroller 130 with timing function at this vitro detection equipment, this microcontroller 130 is used for controlling this checkout equipment testing process, specifically moves and control the load pump absorption second detection reagent for automatic control sample needle at mechanical arm; Can also carry out timing to first reaction time of detecting reagent and detected sample simultaneously, and can the reaction time then, the control sample needle moves along mechanical arm, while start-up loading pump, draw second from container and detect reagent, and by sample needle the second detection reagent is splashed in the reaction cup.
Particularly, second detects reagent is held by special container 104, this container 104 is arranged on the monitor station 100, concrete placement location is not done restriction at this, because load pump connects this container 104 by conduit, therefore, this container 104 can freely arrange on monitor station, is preferably the suitable position that manually picks and places certainly.When carrying out the automatic loading of the second detection reagent, at first, the microprocessor controls sample needle moves along mechanical arm and moves, and finally over against reaction cup; Secondly, microcontroller start-up loading pump is drawn an amount of second from container and is detected reagent, splashes in the reaction cup by sample needle then.Present embodiment is controlled the uptake of the second detection reagent by the microprocessor controls load pump, so that the testing staff is according to detecting the needs adjustment.
Simultaneously, deposit because the second detection reagent need freeze, therefore, second container of present embodiment is preferably and is positioned in the refrigerating plant.
Load pump of the present invention is fixed displacement pump, and for example ram pump or syringe pump specifically can adopt the high precision injection measurement pump of valveless ram pump or T-valve.Valveless ram pump inside is stupalith, when drawing detection reagent by it detection reagent is sticked in the load pump, influences the subsequent detection result in order to avoid detect for a long time; And the high precision injection measurement pump configuration of T-valve is more complicated, it can at first will detect reagent by a valve and draw in pump, and then discharge detection reagent by another valve, this kind load pump can accurately be controlled the addition that detects reagent, if adopt the high precision injection measurement pump of this T-valve, checkout equipment of the present invention can realize detecting adding more fast and accurately of reagent.
Preferably, the structure of Photoelectric Detection module of the present invention as shown in Figure 4, this Photoelectric Detection module comprise a light source 111 that is arranged on reaction cup 200 1 sides, be arranged on reaction cup 200 opposite sides be the photoelectric sensor 113 of predetermined angular with light source over against the optical filter 112 that arranges with the light horizontal direction of living in that light source is launched, predetermined angular is for being α, the big I of α is adjusted according to actual needs, preferable range is [0 °, 30 °].Described light source is used for sending the detection light beam, and described optical filter 112 is used for the detection light beam through reaction cup scattering or transmission is filtered, and described photoelectric sensor 113 is used for receiving the detection light beam after optical filter filters.Detection light beam irradiates detection reagent and detected sample fully react the reaction cup after finishing, owing to contain certain particle in suspension in the reactive material in the reaction cup, when the detection light beam contains the medium of particle in suspension by one, because particle in suspension is to scattering of light effect and optionally absorption, the transmission light intensity is weakened or the scattered intensity enhancing, by the detection to transmitted light or scattered intensity, determine the turbidity of reactive material, and then the content of material composition in the mensuration detected sample, realize the detection of specific protein content.According to the difference of deciding albumen, realize the detection of different test indexs.
Based on above-mentioned vitro detection equipment, the present invention also provides a kind of detection method by above-mentioned vitro detection equipment, as shown in Figure 5, comprises step:
Step S100, detect reagent and the reaction time of detected sample in reaction cup then when detecting first, the microprocessor controls sample needle moves to the reaction cup place along mechanical arm, and aims at the reaction cup center;
Step S200, microcontroller start-up loading pump detect and to draw second the container of reagent and detect reagent from holding second, and it is joined in the reaction cup react;
Step S300, determine the content of specific protein in the detected sample by the turbidity of reactive material in the reaction cup.
Before execution in step S100, at first by the testing staff the manual adding of detected sample being equipped with first in advance detects in the reaction cup of reagent, again reaction cup is inserted in the corresponding Photoelectric Detection module, by Photoelectric Detection module stirring reaction cup, make detected sample and first detect reagent reaction fully in reaction cup.The reaction time then, timer can detect, and sends warning, provides prompting.
Wherein, as shown in Figure 6, after step S300, also comprise:
Step S400, microcontroller are controlled sample needle again and are moved, and sample needle is moved in the next reaction cup, and the second detection reagent is joined in this reaction cup, carry out next group and detect.
In order to make the above embodiment of the present invention more clear, below in conjunction with specific protein detect a kind of implementation process that the present invention is measured specific protein content by turbidimetry and be specifically described.
This embodiment carries out CRP by the c reactive protein detection kit and detects.CRP is proportionate as acute phase protein rising amplitude and the infected degree of human body.CRP can be used for the antidiastole of bacterium and virus infections.In surperficial healthy population, the concentration of CRP is dangerous directly related with generation coronary heart disease.To resist people's c reactive protein antibody sandwich on latex particle, can with serum in c reactive protein generation agglutinating reaction, form antigen antibody complex, its turbidity height is directly proportional with CRP content in the serum when a certain amount of antibody exists.By detecting transmitted light or scattered intensity, can calculate the content of CRP in the serum with reference to multiple spot calibration calibration curve.When detecting, when detecting, first of prepackage 600uL detects reagent R1 in the reaction cup, draw the detected sample (serum or whole blood) of 3uL by sample needle, join in the reaction cup, the Photoelectric Detection module is its mixing, and places 37 ℃ of incubation a period of times, sample needle moves the second detection reagent R2 that draws 60uL then, and splash in the reaction cup, the Photoelectric Detection module is its mixing, and after hatching 10 seconds, read scattered light intensity A1 in continuous 90 seconds, and then calculate corresponding CRP content.The process of above-mentioned detection CRP content only is used for explaining that the present invention measures the method for specific protein content by turbidimetry, and is not used in this method of restriction, because detection method of the present invention can be applied to the detection of multiple specific protein.Such as α acidoglycoprotein, haptoglobin, aPoA 1 etc.
In sum, a kind of semi-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein provided by the invention, when detecting first, timer detects reagent and the reaction time of detected sample in reaction cup then, the microprocessor controls sample needle moves to the reaction cup place, and aims at the reaction cup center; Microcontroller start-up loading pump detects and to draw second the container of reagent and detect reagent from holding second, and it is joined in the reaction cup reacts; Turbidity by reactive material in the reaction cup changes the content of determining specific protein in the detected sample.The present invention has realized the automatic loading of the second detection reagent, saved the operation of the artificial loading second detection reagent, not only save the cost of labor that specific protein detects, saved the manual monitoring link, but also the operation error of having avoided artificial dropping to detect reagent as much as possible impacts to testing result; The present invention has simultaneously improved the detection efficiency of specific protein, has shortened the detection time of specific protein greatly, has vast market prospect.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, for those of ordinary skills, can be improved according to the above description or conversion that all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (8)
1. semi-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, it is characterized in that, comprise a monitor station, described monitor station is provided with the Photoelectric Detection module of band temperature control and agitating function, be placed with the reaction cup of pre-installing the first detection reagent at described Photoelectric Detection module, on described monitor station, also be provided with a mechanical arm, load pump and the container that holds the second detection reagent, one sample needle is installed on the described mechanical arm, one end of described load pump connects described sample needle, the other end connects described container, also is provided with the microcontroller for the described checkout equipment testing process of control on monitor station.
2. employing turbidimetry according to claim 1 is measured the semi-automatic vitro detection equipment of specific protein, it is characterized in that described Photoelectric Detection module includes several.
3. employing turbidimetry according to claim 2 is measured the semi-automatic vitro detection equipment of specific protein, it is characterized in that, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
4. employing turbidimetry according to claim 1 is measured the semi-automatic vitro detection equipment of specific protein, it is characterized in that described load pump is fixed displacement pump.
5. employing turbidimetry according to claim 4 is measured the semi-automatic vitro detection equipment of specific protein, it is characterized in that described fixed displacement pump is ram pump or syringe pump.
6. according to claim 2 automatically to loading the vitro detection equipment that detects reagent, it is characterized in that described Photoelectric Detection module comprises a light source that is arranged on reaction cup one side, be arranged on the reaction cup opposite side and light source is the photoelectric sensor that predetermined angular arranges.
7. the semi-automatic external detection method according to each described vitro detection equipment employing turbidimetry measurement specific protein of claim 1 to 6 is characterized in that, comprises step:
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, the microprocessor controls sample needle moves to the reaction cup place along mechanical arm, and aims at the reaction cup center;
Microcontroller start-up loading pump detects and to draw second the container of reagent and detect reagent from holding second, and it is joined in the reaction cup reacts;
Determine the content of specific protein in the detected sample by the turbidity of reactive material in the reaction cup.
8. measure the semi-automatic external detection method of specific protein according to the described employing turbidimetry of claim 7, it is characterized in that, the step of determining the content of specific protein in the detected sample by the turbidity of reactive material in the reaction cup also comprises afterwards: microcontroller is controlled sample needle again and is moved, sample needle is moved in the next reaction cup, and detect reagent with second and join in this reaction cup, carry out next group and detect.
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Application publication date: 20131009 |