CN102893851A - Butterfly orchid breeding method - Google Patents
Butterfly orchid breeding method Download PDFInfo
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- CN102893851A CN102893851A CN2012102042870A CN201210204287A CN102893851A CN 102893851 A CN102893851 A CN 102893851A CN 2012102042870 A CN2012102042870 A CN 2012102042870A CN 201210204287 A CN201210204287 A CN 201210204287A CN 102893851 A CN102893851 A CN 102893851A
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- 238000009395 breeding Methods 0.000 title claims abstract description 29
- 244000079298 Epidendrum obrienianum Species 0.000 title 1
- 230000035800 maturation Effects 0.000 claims abstract description 18
- 238000009396 hybridization Methods 0.000 claims abstract description 17
- 230000010152 pollination Effects 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000009331 sowing Methods 0.000 claims abstract description 11
- 244000127818 Phalaenopsis amabilis Species 0.000 claims description 32
- 239000003337 fertilizer Substances 0.000 claims description 26
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 238000005516 engineering process Methods 0.000 claims description 20
- 238000005286 illumination Methods 0.000 claims description 18
- 239000002609 medium Substances 0.000 claims description 18
- 235000013399 edible fruits Nutrition 0.000 claims description 17
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 16
- 229910052698 phosphorus Inorganic materials 0.000 claims description 16
- 239000011574 phosphorus Substances 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 13
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000011591 potassium Substances 0.000 claims description 10
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- 238000010899 nucleation Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 241000234295 Musa Species 0.000 claims description 6
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000007943 implant Substances 0.000 claims description 6
- 229940072033 potash Drugs 0.000 claims description 6
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 6
- 235000015320 potassium carbonate Nutrition 0.000 claims description 6
- 239000003643 water by type Substances 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 abstract description 15
- 240000002292 Psychopsis papilio Species 0.000 abstract 3
- 238000011218 seed culture Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000233855 Orchidaceae Species 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 240000008122 Phalaenopsis pulcherrima Species 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 241001517197 Cattleya Species 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
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Abstract
The invention discloses a butterfly orchid breeding method. The butterfly orchid breeding method comprises hybridization pollination, seed culture, young seedling plantation, middle seedling plantation and large seedling plantation. In the fourth month after the hybridization pollination, pods can be harvested. In the fifth-sixth month after aseptic sowing, seedlings can be taken out of a culture bottle and can be planted. The seedlings are planted in a greenhouse. Through control of environment conditions, the seedlings can grow fast. The time from seedling planting to plant maturation is in a range of about 10-12 months. The time from flower forcing to novel breed selection is controlled in 70-100 days. The time of the whole breeding process is controlled in about 22-25 months. The butterfly orchid breeding method can control all processes of the whole breeding process and realizes fast selection of a novel breed.
Description
Technical field
The present invention relates to field of plant breeding, relate to particularly the breeding method of Moth orchid.
Background technology
Moth orchid was described as the queen in the orchid, is that the most ace-high torrid zone is one of blue in the world at present, replaced the orchid that Bowring cattleya becomes the sales volume maximum in 1980.Through the breeding of last 100 years, modern Moth orchid is of a great variety.Before the sixties in last century, the breeding objective of Moth orchid mainly is the cut-flower type, and Moth orchid has become the most extensive potted plant orchid now.Market-oriented pot variety is the compact kind of plant type of selecting from cutting flower variety the earliest, and existing a large amount of suitable potted plant kind brings out now.The Moth orchid breeding method mainly contains:
Crossbreeding, other also have application such as mutation breeding and biotechnology breeding.The proterties intergeneric cross that utilizing intergeneric cross to introduce the Moth orchid germplasm does not have is the important means of the novel kind of initiative Moth orchid, at present most widely used general, the most successful be intergeneric cross between Moth orchid and the Doritis pulcherrima, the outstanding proterties of Doritis pulcherrima is introduced in the Moth orchid, greatly promoted its quality.
Mutation breeding: the at present contribution of mutation breeding in the Moth orchid breeding mainly is to induce the generation polyploid.Screening utilizes occurring in nature to exist the Moth orchid tetraploid plant greatly to promote hybrid Moth orchid quality in the breeding, has cultivated many modern Da Hua type Hybrids.
Biotechnology breeding: utilize the modern genetic engineering technology, certain specific gene is imported in the acceptor material, directionally plant is transformed.
Wherein, Moth orchid need to just can be picked out new varieties with the time in 3 years by traditional breeding method in crossbreeding, and can't guarantee the hapanthous stability of seedling.
Summary of the invention
The object of the present invention is to provide a kind of breeding method of Moth orchid, to solve the above-mentioned problems in the prior art.
Technical scheme provided by the invention is as follows:
A, hybridization pollination: the selection of time hybridization of pollinating between the March to May in every year, can utilize the weather conditions of nature to accelerate the maturation of fruit pod; Next is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%.If temperature is greater than 30 degree, the easy explosion of fruit pod if be lower than 25 degree, particularly is lower than 20 degree, and fruit pod long ripening need 6-7 month, and in temperature range of the present invention, the time is about 4 months, and the not explosion of fruit pod.Humidity is greater than 80%, will cause hot and humidly, easily makes the fruit pod produce germ and causes mutual infection; Humidity is less than 60%, and fruit pod growing way is not good, can reduce its eugenic rate.。
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, at first implant Moth orchid sowing medium, in the time of after planting 35-45 days, move into protocorm bulk-growth medium, increase the growing space, promote growth; After cultivating 35-45 days, change to the seedling growth medium; It is the outer plantation of extensible bottle that seedling is cultivated 45-60 days;
The seedling plantation of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, EC value (being used for measuring the numerical value of soluble-salt concentration in the solution) is controlled at 0.5-0.6(mS/cm, lower with) between, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ± 1 ℃, keep ventilating, promote the Fast Growth of seedling;
The middle seed of D, seedling is planted: seedling plantation about 90-120 days, displant in 2.5 cun basins, watered once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 35-45ppm, the EC value is controlled between the 0.6-0.7, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%;
The seedlings plantation of E, seedling: after middle seed was planted 90-120 days, the basin that changes 3.5 cun carried out the seedlings plantation, waters fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 45-55ppm, temperature 26-30 ℃, light can be controlled in 18000-20000Lux, and relative moisture is 60-80%;
The flower forcing of F, seedling: seedlings are after 120-150 days plant maturation, begin to carry out flower forcing and process, 1-2 is individual month before processing, and improves phosphorus potash fertilizer, the day temperature control is at 25 ℃ ± 1 ℃, night temperature control is at 18-20 ℃, and luminous intensity is controlled at after 20000-25000. flower forcing 20-40 days, moves to the room of blooming, temperature is at 26-28 ℃, relative moisture is at 60-85%, and luminous intensity is at 20000-25000Lux, Accelerate bloom speed;
G, select new varieties.
More preferably, in the step 1, temperature is 28 ± 1 ℃.
In preferred embodiment of the present invention, the sowing medium is:
Spend precious No. 1 2.-2.5g/l; Peptone 1.8-2.5g/l;
Active carbon 1.8-2.5g/l; Sucrose 18-25g/l;
Agar 6-8g/l;
PH 5.5-5.8。
In preferred embodiment of the present invention, protocorm bulk-growth medium is:
Spend precious No. 1 2.2-2.8g/l; Peptone 1.8-2.5g/l;
Banana 45-55g/l; Active carbon 0.8-1.5g/l;
Agar 6-8g/l; Sucrose 8-12g/l;
PH 5.3-5.5。
In preferred embodiment of the present invention, the seedling growth medium is:
Spend precious No. 1 2.8-3.5g/l; Peptone 2.8-3.5g/l;
Banana 90-110g/l; Active carbon 0.8-1.5g/l;
Citric acid 28-35g/l; Sucrose 12-18g/l;
Agar 6-8g/l;
PH 5.1-5.3。
Above sowing medium, protocorm bulk-growth medium, seedling growth medium also can adopt other moth orchid culture medium of the prior art, but preferably for adopting above prescription.
The present invention is by the control to each link in the whole breeding process, can just can pick out new varieties quickly, as, the link of hybridization pollination can be controlled in 4 months fruit pod of just can gathering, but aseptic seeding 5-6 month and Planting out of test-tube, the plantation of seedling in the greenhouse, by the control of environmental condition, can be conducive to seedling strain Fast Growth, the plantation in seedling stage was to ripe about 10-12 month, flower forcing to selecting of new varieties is controlled at 70-100 days, and whole breeding process was controlled at about 22-25 month.The present invention can carry out selecting of Moth orchid new varieties fast, shorten the Moth orchid breeding time limit, and each link in plantation reaches relevant technical guarantee seedling strain healthy mature by the ring control, and the flower of leaving can guarantee that the Characters of seedling is relatively stable, is beneficial to and selects.
Embodiment
A, hybridization pollination: the maternal Moth orchid JB2031(of being numbers); Male parent is JB2064
At first being the selection of time, being chosen in the hybridization of pollinating between annual March to May, secondly is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%.Pollination is controlled at 4 months to fruit pod maturation.
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, what at first implant is Moth orchid sowing medium (table 1), in the time of after planting 40 days, moves into the medium (table 2) that promotes the protocorm bulk-growth, increases the growing space, promotes growth; After cultivating 40 days, change to the medium (table 3) that promotes the seedling growth; Seedling is cultivated and was namely shifted out the outer plantation of bottle in 50 days.
The seedling plantation of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, and the EC value is controlled between the 0.5-0.6, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ℃, keeps ventilating, and promotes the Fast Growth of seedling.
The middle seed of D, seedling is planted: the seedling plantation is after 100 days, displant in 2.5 cun basins, watered (containing liquid fertilizer) once every 7-10 days, the about 40ppm of fertilizer concentration, the EC value is controlled at 0.6-0.7(mS/cm) between, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%.
The seedlings plantation of E, seedling: after middle seed was planted 105 days, the basin that changes 3.5 cun carried out the seedlings plantation, waters fertilizer once every 7-10 days, and rich water concentration is 26-30 ℃ of 50ppm. temperature, and light can be controlled in 18000-20000Lux, and relative moisture is 60-80%.
The flower forcing technology of F, seedling: seedlings are after 120-150 days plant maturation, begin to carry out flower forcing and process, 1-2 is individual month before processing, and improves phosphorus potash fertilizer, the day temperature control is about 25 ℃, night temperature control is at 18-20 ℃, and luminous intensity is controlled at after 20000-25000. flower forcing 20-40 days, moves to the room of blooming, temperature is at 26-28 ℃, relative moisture is at 60-85%, and luminous intensity is at 20000-25000Lux, Accelerate bloom speed.
G, select new varieties.
Table 1 Moth orchid sowing medium
| Spend precious No. 1 | Peptone | Active carbon | Sucrose | Agar | PH |
| 2.3g/l | 2g/l | 2g/l | 20g/l | 6-8g/l | 5.5-5.8 |
Table 2 protocorm bulk-growth medium
| Spend precious No. 1 | Peptone | Banana | Active carbon | Sucrose | Agar | PH |
| 2.5g/l | 2g/l | 50g/l | 1g/l | 10g/l | 6-8g/l | 5.3-5.5 |
Table 3 seedling growth medium
| Spend precious No. 1 | Peptone | Banana | Active carbon | Sucrose | Citric acid | Agar | PH |
| 3g/l | 3g/l | 100g/l | 1g/l | 15g/l | 30g/l | 6-8g/l | 5.1-5.3 |
Embodiment 2
Operation and embodiment 1 are basic identical, and difference is, in the hybridization pollination: female parent is JB2083; Male parent is JB2068
A, at first being the selection of time, being chosen in the hybridization of pollinating between annual March to May, secondly is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%.Pollination is controlled at about 4 months to fruit pod maturation.
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, what at first implant is Moth orchid sowing medium (table 1), in the time of after planting 35 days, moves into the medium (table 2) that promotes the protocorm bulk-growth, increases the growing space, promotes growth; After cultivating 41 days, change to the medium (table 3) that promotes the seedling growth; Seedling is cultivated the outer plantation of extensible bottle in 45 days.
The seedling planting technology of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, and the EC value is controlled between the 0.5-0.6, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ℃, keeps ventilating, and promotes the Fast Growth of seedling.
The middle seed planting technology of D, seedling: seedling plantation about 90-120 days, displant in 2.5 cun basins, watered (containing liquid fertilizer) once every 7-10 days, the about 40ppm of fertilizer concentration, the EC value is controlled between the 0.6-0.7, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%.
The seedlings planting technology of E, seedling: after middle seed was planted 90-120 days, the basin that changes 3.5 cun carries out the seedlings plantation, waters fertilizer once every 7-10 days, and rich water concentration is 26-30 ℃ of 50ppm. temperature, light can be controlled in 18000-20000Lux, and relative moisture is 60-80%.
The flower forcing technology of F, seedling: seedlings are after 120-150 days plant maturation, begin to carry out flower forcing and process, 1-2 is individual month before processing, and improves phosphorus potash fertilizer, the day temperature control is about 25 ℃, night temperature control is at 18-20 ℃, and luminous intensity is controlled at after 20000-25000. flower forcing 20-40 days, moves to the room of blooming, temperature is at 26-28 ℃, relative moisture is at 60-85%, and luminous intensity is at 20000-25000Lux, Accelerate bloom speed.
Selecting of G, new varieties:
Embodiment 3
Operation and embodiment 1 are basic identical, and difference is, in the hybridization pollination: female parent is JB2191; Male parent is JB2088
A, hybridization pollination: at first being the selection of time, being chosen in the hybridization of pollinating between annual March to May, secondly is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%.Pollination is controlled at 4 months to fruit pod maturation.
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, what at first implant is Moth orchid sowing medium (table 1), in the time of after planting 35-45 days, moves into the medium (table 2) that promotes the protocorm bulk-growth, increase the growing space, promote growth; After cultivating 35-45 days, change to the medium (table 3) that promotes the seedling growth; It is the outer plantation of extensible bottle (taking approximately half a year from being seeded into bottle outlet) that seedling is cultivated 45-60 days.
The seedling planting technology of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, and the EC value is controlled between the 0.5-0.6, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ℃, keeps ventilating, and promotes the Fast Growth of seedling.
The middle seed planting technology of D, seedling: seedling plantation about 90-120 days, displant in 2.5 cun basins, watered (containing liquid fertilizer) once every 7-10 days, the about 40ppm of fertilizer concentration, the EC value is controlled between the 0.6-0.7, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%.
The seedlings planting technology of E, seedling: after middle seed was planted 90-120 days, the basin that changes 3.5 cun carries out the seedlings plantation, waters fertilizer once every 7-10 days, and rich water concentration is 26-30 ℃ of 50ppm. temperature, light can be controlled in 18000-20000Lux, and relative moisture is 60-80%.
The flower forcing technology of F, seedling: seedlings are after 120-150 days plant maturation, begin to carry out flower forcing and process, 1-2 is individual month before processing, and improves phosphorus potash fertilizer, the day temperature control is about 25 ℃, night temperature control is at 18-20 ℃, and luminous intensity is controlled at after 20000-25000. flower forcing 20-40 days, moves to the room of blooming, temperature is at 26-28 ℃, relative moisture is at 60-85%, and luminous intensity is at 20000-25000Lux, Accelerate bloom speed.
Selecting of G, new varieties:
Time sees Table 4,
Embodiment 4
Operation and embodiment 1 are basic identical, and difference is, in the hybridization pollination: female parent is JB2088; Male parent is JB2094
A, hybridization pollination: at first being the selection of time, being chosen in the hybridization of pollinating between annual March to May, secondly is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%.Pollination is controlled at 4 months to fruit pod maturation.
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, what at first implant is Moth orchid sowing medium (table 1), in the time of after planting 35-45 days, moves into the medium (table 2) that promotes the protocorm bulk-growth, increase the growing space, promote growth; After cultivating 35-45 days, change to the medium (table 3) that promotes the seedling growth; It is the outer plantation of extensible bottle (taking approximately half a year from being seeded into bottle outlet) that seedling is cultivated 45-60 days.
The seedling planting technology of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, and the EC value is controlled between the 0.5-0.6, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ℃, keeps ventilating, and promotes the Fast Growth of seedling.
The middle seed planting technology of D, seedling: seedling plantation about 90-120 days, displant in 2.5 cun basins, watered (containing liquid fertilizer) once every 7-10 days, the about 40ppm of fertilizer concentration, the EC value is controlled between the 0.6-0.7, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%.
The seedlings planting technology of E, seedling: after middle seed was planted 90-120 days, the basin that changes 3.5 cun carries out the seedlings plantation, waters fertilizer once every 7-10 days, and rich water concentration is 26-30 ℃ of 50ppm. temperature, light can be controlled in 18000-20000Lux, and relative moisture is 60-80%.
The flower forcing technology of F, seedling: seedlings are after 120-150 days plant maturation, begin to carry out flower forcing and process, 1-2 is individual month before processing, and improves phosphorus potash fertilizer, the day temperature control is about 25 ℃, night temperature control is at 18-20 ℃, and luminous intensity is controlled at after 20000-25000. flower forcing 20-40 days, moves to the room of blooming, temperature is at 26-28 ℃, relative moisture is at 60-85%, and luminous intensity is at 20000-25000Lux, Accelerate bloom speed.
Selecting of G, new varieties:
Time sees Table 4,
Table 4 breeding time
Claims (5)
1. Moth orchid breeding method may further comprise the steps:
A, hybridization pollination: the selection of time hybridization of between the March to May in every year, pollinating; Next is the control of temperature and humidity, and temperature is controlled at 28 ± 2 ℃, and humidity is controlled at 60-80%;
The cultivation of B, seed: utilize the aseptic seeding technology that the fruit pod of maturation is sowed, at first implant Moth orchid sowing medium, in the time of after planting 35-45 days, move into protocorm bulk-growth medium, increase the growing space, promote growth; After cultivating 35-45 days, change to the seedling growth medium; It is the outer plantation of extensible bottle that seedling is cultivated 45-60 days;
The seedling plantation of C, seedling: the seedling of firm bottle outlet is planted in 1.5 cun basins, illumination is controlled at 5000-7000Lux, after one month, executed thin fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 30-40ppm, and the EC value is controlled between the 0.5-0.6, and illumination is controlled at 8000-10000Lux, temperature is controlled at 25 ± 1 ℃, keeps ventilating, and promotes the Fast Growth of seedling;
The middle seed of D, seedling is planted: seedling plantation about 90-120 days, displant in 2.5 cun basins, watered once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 35-45ppm, the EC value is controlled between the 0.6-0.7, illumination is increased to 12000-15000Lux, and temperature is controlled at 25-28 ℃, and relative moisture is controlled at 60-85%;
The seedlings plantation of E, seedling: after middle seed was planted 90-120 days, the basin that changes 3.5 cun carried out the seedlings plantation, waters fertilizer once every 7-10 days, nitrogen, phosphorus, potassium concn respectively are 45-55ppm, temperature 26-30 ℃, light is controlled at 18000-20000Lux, and relative moisture is 60-80%;
The flower forcing of F, seedling: seedlings begin to carry out flower forcing and process after 120-150 days plant maturation, and 1-2 is individual month before processing, improve phosphorus potash fertilizer, the day temperature control is at 25 ℃ ± 1 ℃, and night temperature control is at 18-20 ℃, and luminous intensity is controlled at 20000-25000, after flower forcing 20-40 days, move to the room of blooming, temperature is at 26-28 ℃, and relative moisture is at 60-85%, luminous intensity is at 20000-25000Lux, Accelerate bloom speed;
G, select new varieties.
2. a kind of Moth orchid breeding method as claimed in claim 1, it is characterized in that: in the step 1, temperature is 28 ± 1 ℃.
3. a kind of Moth orchid breeding method as claimed in claim 1 is characterized in that, the sowing medium is:
Spend precious No. 1 2.-2.5g/l; Peptone 1.8-2.5g/l;
Active carbon 1.8-2.5g/l; Sucrose 18-25g/l;
Agar 6-8g/l;
PH 5.5-5.8。
4. a kind of Moth orchid breeding method as claimed in claim 1 is characterized in that, protocorm bulk-growth medium is:
Spend precious No. 1 2.2-2.8g/l; Peptone 1.8-2.5g/l;
Banana 45-55g/l; Active carbon 0.8-1.5g/l;
Agar 6-8g/l; Sucrose 8-12g/l;
PH 5.3-5.5。
5. a kind of Moth orchid breeding method as claimed in claim 1 is characterized in that the seedling growth medium is:
Spend precious No. 1 2.8-3.5g/l; Peptone 2.8-3.5g/l;
Banana 90-110g/l; Active carbon 0.8-1.5g/l;
Citric acid 28-35g/l; Sucrose 12-18g/l;
Agar 6-8g/l;
PH 5.1-5.3。
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| CN2012102042870A CN102893851B (en) | 2012-06-19 | 2012-06-19 | Butterfly orchid breeding method |
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| CN2012102042870A CN102893851B (en) | 2012-06-19 | 2012-06-19 | Butterfly orchid breeding method |
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| CN102893851B CN102893851B (en) | 2013-11-27 |
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| CN103168609A (en) * | 2013-04-18 | 2013-06-26 | 贵州省农作物品种资源研究所 | Pleione seed original habitat natural sowing method |
| CN103416144A (en) * | 2013-07-16 | 2013-12-04 | 武朝菊 | Method for cultivating high-quality phalaenopsis |
| CN103975858A (en) * | 2014-05-29 | 2014-08-13 | 天津滨城天龙农业科技有限公司 | Hormone-free culture medium for culturing high-quality butterfly orchid seedlings and application of hormone-free culture medium |
| CN104813937A (en) * | 2015-05-11 | 2015-08-05 | 福建农林大学 | Regenerating and rapid propagating method for liparis nervosa |
| CN106810368A (en) * | 2017-01-23 | 2017-06-09 | 中国林业科学研究院林业研究所 | A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium |
| CN108243944A (en) * | 2018-01-24 | 2018-07-06 | 青州市亚泰农业科技有限公司 | A kind of iris rapid breeding method and fine quality tissue culture propagation |
| CN108651444A (en) * | 2018-05-22 | 2018-10-16 | 山东省农业科学院蔬菜花卉研究所 | A kind of iris pollen preserves and breeding method |
| CN109258377A (en) * | 2018-11-14 | 2019-01-25 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | A kind of method of bean cotyledon orchid species matter initiative |
| CN109618918A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | Open up the breeding method of shape cherry iris |
| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN111543322A (en) * | 2020-05-27 | 2020-08-18 | 山东省农业科学院蔬菜花卉研究所 | Method for cultivating and tissue culturing and propagating new species of virus-free phalaenopsis |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168609A (en) * | 2013-04-18 | 2013-06-26 | 贵州省农作物品种资源研究所 | Pleione seed original habitat natural sowing method |
| CN103168609B (en) * | 2013-04-18 | 2014-09-17 | 贵州省农作物品种资源研究所 | Pleione seed original habitat natural sowing method |
| CN103416144A (en) * | 2013-07-16 | 2013-12-04 | 武朝菊 | Method for cultivating high-quality phalaenopsis |
| CN103975858A (en) * | 2014-05-29 | 2014-08-13 | 天津滨城天龙农业科技有限公司 | Hormone-free culture medium for culturing high-quality butterfly orchid seedlings and application of hormone-free culture medium |
| CN104813937A (en) * | 2015-05-11 | 2015-08-05 | 福建农林大学 | Regenerating and rapid propagating method for liparis nervosa |
| CN106810368A (en) * | 2017-01-23 | 2017-06-09 | 中国林业科学研究院林业研究所 | A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium |
| CN108243944A (en) * | 2018-01-24 | 2018-07-06 | 青州市亚泰农业科技有限公司 | A kind of iris rapid breeding method and fine quality tissue culture propagation |
| CN108651444A (en) * | 2018-05-22 | 2018-10-16 | 山东省农业科学院蔬菜花卉研究所 | A kind of iris pollen preserves and breeding method |
| CN108651444B (en) * | 2018-05-22 | 2020-11-27 | 山东省农业科学院蔬菜花卉研究所 | Preservation and breeding method for phalaenopsis pollen |
| CN109258377A (en) * | 2018-11-14 | 2019-01-25 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | A kind of method of bean cotyledon orchid species matter initiative |
| CN109258377B (en) * | 2018-11-14 | 2022-05-17 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Method for creating germplasm of watercress |
| CN109618918A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | Open up the breeding method of shape cherry iris |
| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN111543322A (en) * | 2020-05-27 | 2020-08-18 | 山东省农业科学院蔬菜花卉研究所 | Method for cultivating and tissue culturing and propagating new species of virus-free phalaenopsis |
| CN111543322B (en) * | 2020-05-27 | 2021-07-06 | 山东省农业科学院蔬菜花卉研究所 | Method for cultivating and tissue culturing and propagating new species of virus-free phalaenopsis |
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