CN102095771A - Ampere detection pool and ampere detection method for saccharide carbohydrate - Google Patents
Ampere detection pool and ampere detection method for saccharide carbohydrate Download PDFInfo
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- CN102095771A CN102095771A CN2011100241061A CN201110024106A CN102095771A CN 102095771 A CN102095771 A CN 102095771A CN 2011100241061 A CN2011100241061 A CN 2011100241061A CN 201110024106 A CN201110024106 A CN 201110024106A CN 102095771 A CN102095771 A CN 102095771A
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- working electrode
- amperometric detection
- copper
- pond body
- cell
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- 238000001514 detection method Methods 0.000 title claims abstract description 51
- -1 saccharide carbohydrate Chemical class 0.000 title abstract description 7
- 239000010949 copper Substances 0.000 claims abstract description 32
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910052802 copper Inorganic materials 0.000 claims abstract description 23
- 150000001720 carbohydrates Chemical class 0.000 claims description 23
- 235000014633 carbohydrates Nutrition 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000002699 waste material Substances 0.000 claims description 6
- 239000012491 analyte Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000006479 redox reaction Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000002829 reductive effect Effects 0.000 abstract description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000012490 blank solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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Abstract
The invention discloses an ampere detection pool and an ampere detection method for saccharide carbohydrate. A working electrode made of copper is used for the ampere detection of constant potential of the saccharide carbohydrate, so that the detection sensitivity to the constant potential of the saccharide carbohydrate on the working electrode can be improved, and the signal intensity is increased. Meanwhile, in the detection of the saccharide carbohydrate, the working electrode made of the copper has high stability and repeatability, and signal attenuation is reduced.
Description
Technical field
The present invention relates to the Amperometric Detection Coupled technology of saccharide compound, more particularly, relate to a kind of sugary carbohydrates amperometric detection cell and detection method thereof.
Background technology
Because sugary carbohydrates can be oxidized on noble metal (gold, platinum etc.) electrode, therefore, the working electrode of traditional sugary carbohydrates amperometric detection cell generally adopts gold as manufacturing materials.But, in actual use because the surface that the product of oxidation reaction also can poison electrode, make material be the working electrode of gold under the constant potential condition, very easily contaminated when measuring saccharide compound, to such an extent as to suppressed further detection to measured object.See also shown in Figure 1, Fig. 1 carries out the I-T curve map that constant potential detects for glucose on golden working electrode, we can find out clearly that signal decays along with the increase of sample introduction number of times obviously from Fig. 1, this be since the surface of golden working electrode be passivated cause, finally cause the detection signal that obtains less, sensitivity and less stable do not have good repeatability.
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Summary of the invention
At the above-mentioned shortcoming that exists in the prior art, the purpose of this invention is to provide a kind of sugary carbohydrates amperometric detection cell and detection method thereof, also improve the sensitivity that detects in order to stability, the repeatability that guarantees to detect.
For achieving the above object, the present invention adopts following technical scheme:
On the one hand, sugary carbohydrates amperometric detection cell of the present invention, comprise the pond body, be located at pond body below the working electrode seat, be located at the flow cell between pond body and the working electrode seat, the pond body is provided with carrier fluid inlet and the contrast electrode hole that is connected with flow cell respectively, contrast electrode is equipped with contrast electrode in the hole, and contrast electrode hole one side also has waste liquid outlet; Also be provided with on the working electrode seat and the contacted working electrode of flow cell, working electrode is a copper.
Described flow cell is by being located between pond body and the working electrode seat and the middle film that is provided with cavity is constituted.
Described working electrode is 99.99% copper.
On the other hand, sugary carbohydrates Amperometric Detection Coupled method of the present invention, carrier fluid inlet by amperometric detection cell flows through amperometric detection cell with strong basicity moving phase with material to be analyzed, when material to be analyzed passes through the working electrode surface of amperometric detection cell, at material is on the working electrode of copper, redox reaction takes place under the constant potential that is applied, and by galvanochemistry ampere detector testing result; Discharge by the analyte of waste liquid outlet after detection reaction.
The film that is provided with cavity in the middle of described flow cell adopts is located between pond body and the working electrode seat and constitutes.
Described working electrode is selected 99.99% copper work for use.
In technique scheme, sugary carbohydrates amperometric detection cell of the present invention and detection method thereof, by adopting with copper is the working electrode of material carries out constant potential to sugary carbohydrates Amperometric Detection Coupled, can improve the sugary carbohydrates sensitivity that constant potential detects on working electrode, and the increase signal intensity, simultaneously, copper working electrode also has stability preferably in the sugary carbohydrates context of detection, signal attenuation is little, has splendid repeatability.
Description of drawings
Fig. 1 is that glucose carries out the I-T curve map that constant potential detects on traditional golden working electrode;
Fig. 2 is the structure cut-open view of sugary carbohydrates amperometric detection cell of the present invention;
Fig. 3 is that glucose carries out the I-T curve map that constant potential detects on copper working electrode of the present invention;
Fig. 4 is that Fig. 1 and Fig. 3 merge comparison diagram.
Embodiment
Further specify technical scheme of the present invention below in conjunction with drawings and Examples.
Please in conjunction with shown in Figure 2, sugary carbohydrates amperometric detection cell of the present invention comprises pond body 1, is located at the working electrode seat 2 of pond body 1 below, is located at the flow cell 3 between pond body 1 and the working electrode seat 2, pond body 1 is provided with carrier fluid inlet 4 and the contrast electrode hole 5 that is connected with flow cell 3 respectively, contrast electrode 6 is installed in the contrast electrode hole 5, and contrast electrode hole 5 one sides also have waste liquid outlet 7; Also be provided with on the working electrode seat 2 and flow cell 3 contacted working electrodes 8, the material of this working electrode 8 is a copper.As a preferred embodiment, working electrode 8 can be selected 99.99% copper work for use.As another embodiment, this flow cell 3 is by being located between 82 of pond body 1 and the working electrodes and the middle film 10 that is provided with cavity 9 is constituted.
The principle of work of this amperometric detection cell is as follows:
Adopt galvanochemistry Amperometric Detection Coupled method, carrier fluid inlet by amperometric detection cell will flow through amperometric detection cell as the material to be analyzed of sugary carbohydrates with strong basicity moving phase, when material to be analyzed passes through the working electrode surface of amperometric detection cell, at material is on the working electrode of copper, redox reaction takes place under the constant potential that is applied, by galvanochemistry ampere detector testing result; Discharge by the analyte as sugary carbohydrates of waste liquid outlet after detection reaction.
With glucose is example, the detection principle of glucose on copper working electrode 8 is: the metallic copper electrode in strong base solution along with the increase of current potential, can be oxidized to higher valence state gradually, in 0.1M NaOH be respectively :-0.43V Cu (0)/Cu (I),-0.18 Cu (0)/Cu (II) ,+0.50V Cu (II)/Cu (III).Wherein, the oxidation of Cu (II)/Cu (III) is irreversible in the NaOH blank solution.When copper working electrode 8 detects sugary carbohydrates during at+0.50V current potential, because sugar itself is reductive agent,, tangible electrochemical signals can occur, and repeatability is fine so sugar is easy to the oxidation by Cu (III) under this current potential.
See also shown in Figure 3, the I-T curve from Fig. 3 as can be seen, it still is apparent in view that the constant potential of glucose on copper working electrode 8 detects, its signal intensity is also bigger, the while copper electrode has stable preferably in the context of detection of glucose.
See also shown in Figure 4, now the constant potential I-T curve of glucose on golden working electrode and copper working electrode 8 made comparisons, under the detecting pattern of constant potential, can further specify by Fig. 4: compare with traditional golden working electrode, it is bigger, highly sensitive to adopt copper working electrode 8 to have a detection signal, and stability and repeatability are better, plurality of advantages such as signal attenuation is little, effect is very remarkable.
Because the principle of sugary carbohydrates Amperometric Detection Coupled method of the present invention is identical with above-mentioned detection cell essence, repeats no more inferior.
Those of ordinary skill in the art will be appreciated that, above embodiment is used for illustrating the present invention, and be not to be used as limitation of the invention, as long as in connotation scope of the present invention, all will drop in claims scope of the present invention variation, the modification of the above embodiment.
Claims (6)
1. sugary carbohydrates amperometric detection cell is characterized in that:
Comprise the pond body, be located at pond body below the working electrode seat, be located at the flow cell between pond body and the working electrode seat, the pond body is provided with carrier fluid inlet and the contrast electrode hole that is connected with flow cell respectively, contrast electrode is equipped with contrast electrode in the hole, and contrast electrode hole one side also has waste liquid outlet; Also be provided with on the working electrode seat and the contacted working electrode of flow cell, working electrode is a copper.
2. sugary carbohydrates amperometric detection cell as claimed in claim 1 is characterized in that:
Described flow cell is by being located between pond body and the working electrode seat and the middle film that is provided with cavity is constituted.
3. sugary carbohydrates amperometric detection cell as claimed in claim 1 is characterized in that:
Described working electrode is 99.99% copper.
4. sugary carbohydrates Amperometric Detection Coupled method is characterized in that:
Adopt galvanochemistry Amperometric Detection Coupled method, carrier fluid inlet by amperometric detection cell flows through amperometric detection cell with strong basicity moving phase with material to be analyzed, when material to be analyzed passes through the working electrode surface of amperometric detection cell, at material is on the working electrode of copper, redox reaction takes place under the constant potential that is applied, and by galvanochemistry ampere detector testing result; Discharge by the analyte of waste liquid outlet after detection reaction.
5. sugary carbohydrates Amperometric Detection Coupled method as claimed in claim 4 is characterized in that:
The film that is provided with cavity in the middle of described flow cell adopts is located between pond body and the working electrode seat and constitutes.
6. sugary carbohydrates Amperometric Detection Coupled method as claimed in claim 4 is characterized in that:
Described working electrode is selected 99.99% copper work for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100241061A CN102095771A (en) | 2011-01-21 | 2011-01-21 | Ampere detection pool and ampere detection method for saccharide carbohydrate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100241061A CN102095771A (en) | 2011-01-21 | 2011-01-21 | Ampere detection pool and ampere detection method for saccharide carbohydrate |
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| Publication Number | Publication Date |
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| CN102095771A true CN102095771A (en) | 2011-06-15 |
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| CN2011100241061A Pending CN102095771A (en) | 2011-01-21 | 2011-01-21 | Ampere detection pool and ampere detection method for saccharide carbohydrate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983720A (en) * | 2014-06-03 | 2014-08-13 | 上海理工大学 | Electrochemical thin layer flow measuring pool |
| CN105229449A (en) * | 2013-02-08 | 2016-01-06 | 霍夫曼-拉罗奇有限公司 | Automatic analysing apparatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59180354A (en) * | 1983-03-30 | 1984-10-13 | Shimadzu Corp | Electrochemical detection of liquid chromatograph |
| US4654125A (en) * | 1983-03-01 | 1987-03-31 | Rall Gerhard G | Method and apparatus for electrochemical detection |
-
2011
- 2011-01-21 CN CN2011100241061A patent/CN102095771A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4654125A (en) * | 1983-03-01 | 1987-03-31 | Rall Gerhard G | Method and apparatus for electrochemical detection |
| JPS59180354A (en) * | 1983-03-30 | 1984-10-13 | Shimadzu Corp | Electrochemical detection of liquid chromatograph |
Non-Patent Citations (1)
| Title |
|---|
| 叶建农等: "蜂蜜含糖量的高效毛细管电泳测定研究", 《分析测试学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105229449A (en) * | 2013-02-08 | 2016-01-06 | 霍夫曼-拉罗奇有限公司 | Automatic analysing apparatus |
| CN105229449B (en) * | 2013-02-08 | 2018-10-12 | 霍夫曼-拉罗奇有限公司 | Automatic analysing apparatus |
| US10302641B2 (en) | 2013-02-08 | 2019-05-28 | Roche Diagnostics Operations, Inc. | Automatic analysis device |
| CN103983720A (en) * | 2014-06-03 | 2014-08-13 | 上海理工大学 | Electrochemical thin layer flow measuring pool |
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Application publication date: 20110615 |