BRPI0516955A - ensaio genÈmico - Google Patents
ensaio genÈmicoInfo
- Publication number
- BRPI0516955A BRPI0516955A BRPI0516955-0A BRPI0516955A BRPI0516955A BR PI0516955 A BRPI0516955 A BR PI0516955A BR PI0516955 A BRPI0516955 A BR PI0516955A BR PI0516955 A BRPI0516955 A BR PI0516955A
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- acid sequence
- probe
- providing
- markers
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Nanotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
ENSAIO GENÈMICO. Trata-se de um método para detectar uma seqüência de ácido nuclélco em uma amostra genómica que inclui: proporcionar a amostra genómica que contém uma seqúência de ácido nucléico alvo de um ácido nucléico dúplex; proporcionar uma sonda que contém uma seqúência de ácido nucléico de sonda; proporcionar uma mistura de hibridização; irradiar a mistura incubada com radiação eficaz para estimular pelo menos alguns dos marcadores a emitir energia; e detectar a partir de um sinal fluorescente se a sonda se emparelha perfeitamente na seqúência de ácido nucléico alvo, onde a detecção é realizada dentro de sessenta minutos da mistura de hibridização proporcionada, e o método é realizado sem desnaturação e sem amplificação por PCR do ácido nucléico dúplex. Um kit para praticar o método inclui a sonda, o agente promotor de hibridização, e marcadores.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61267004P | 2004-09-24 | 2004-09-24 | |
PCT/IB2005/053162 WO2006033088A2 (en) | 2004-09-24 | 2005-09-23 | Genomic assay |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0516955A true BRPI0516955A (pt) | 2008-09-30 |
Family
ID=36000782
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0516955-0A BRPI0516955A (pt) | 2004-09-24 | 2005-09-23 | ensaio genÈmico |
Country Status (14)
Country | Link |
---|---|
US (1) | US8486622B2 (pt) |
EP (1) | EP1815010A2 (pt) |
JP (1) | JP2008514197A (pt) |
KR (1) | KR20070062575A (pt) |
CN (1) | CN101031659A (pt) |
AU (1) | AU2005286084C1 (pt) |
BR (1) | BRPI0516955A (pt) |
CA (1) | CA2581087C (pt) |
IL (1) | IL182107A0 (pt) |
NZ (1) | NZ554538A (pt) |
RU (1) | RU2464320C2 (pt) |
SG (1) | SG155995A1 (pt) |
WO (1) | WO2006033088A2 (pt) |
ZA (1) | ZA200703326B (pt) |
Families Citing this family (24)
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US7960357B2 (en) | 2005-10-07 | 2011-06-14 | California Institute Of Technology | PKR activation via hybridization chain reaction |
WO2008144562A1 (en) * | 2007-05-16 | 2008-11-27 | California Institute Of Technology | A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways |
US20100021904A1 (en) * | 2008-05-21 | 2010-01-28 | Pierce Niles A | Shielded cross-linking probes |
US20100021901A1 (en) * | 2008-05-22 | 2010-01-28 | Peng Yin | Compositions and methods for detecting analytes |
US8658780B2 (en) | 2010-05-18 | 2014-02-25 | California Institute Of Technology | Triggered covalent probes for imaging and silencing genetic expression |
US9834439B2 (en) | 2010-07-20 | 2017-12-05 | California Institute Of Technology | Biomolecular self-assembly |
US8962241B2 (en) * | 2010-07-20 | 2015-02-24 | California Institute Of Technology | Triggered molecular geometry based bioimaging probes |
US8877438B2 (en) | 2010-07-20 | 2014-11-04 | California Institute Of Technology | Self-assembled polynucleotide structure |
US8980553B2 (en) * | 2011-04-02 | 2015-03-17 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
US9145580B2 (en) * | 2011-04-02 | 2015-09-29 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
SG11201406010QA (en) * | 2012-03-29 | 2014-11-27 | Mitsubishi Rayon Co | MICROARRAY FOR DETECTION OF MUTATIONS IN β-GLOBIN GENES AND DETECTION METHOD THEREOF |
US9856472B2 (en) | 2013-07-01 | 2018-01-02 | California Institute Of Technology | Small conditional RNAs |
AU2014318731A1 (en) * | 2013-09-11 | 2016-03-17 | Agenus Inc. | High throughput screening for biomolecules |
JP6300263B2 (ja) * | 2013-09-25 | 2018-03-28 | 学校法人甲南学園 | 核酸鎖の四重螺旋構造の検出方法 |
RU2577107C2 (ru) * | 2013-10-04 | 2016-03-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Способ поиска белков-мишеней, запускающих процесс канцерогенеза, в образцах тканей индивидуального пациента, для последующей противоопухолевой фармакотерапии |
WO2017070693A1 (en) * | 2015-10-23 | 2017-04-27 | University Of Utah Research Foundation | Methods and systems for detecting variations in dna |
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KR102110999B1 (ko) * | 2016-01-26 | 2020-05-14 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 시그널을 제공하는 방법 |
RU2637310C1 (ru) * | 2016-06-08 | 2017-12-04 | Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) | Флуоресцентно-меченые дезоксиуридинтрифосфаты |
WO2018009463A2 (en) | 2016-07-05 | 2018-01-11 | California Institute Of Technology | Fractional initiator hybridization chain reaction |
WO2018044939A1 (en) | 2016-08-30 | 2018-03-08 | California Institute Of Technology | Immunohistochemistry via hybridization chain reaction |
WO2018183621A1 (en) * | 2017-03-30 | 2018-10-04 | Life Technologies Corporation | Quantification of ngs dna by adapter sequence |
CN112162027B (zh) * | 2020-09-21 | 2023-08-18 | 上海市计量测试技术研究院 | 一种基于三嵌段探针的电化学传感器及其在检测转基因双链rna中的应用 |
US11873485B2 (en) | 2021-01-26 | 2024-01-16 | California Institute Of Technology | Allosteric conditional guide RNAs for cell-selective regulation of CRISPR/Cas |
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2005
- 2005-09-23 RU RU2007115421/10A patent/RU2464320C2/ru not_active IP Right Cessation
- 2005-09-23 WO PCT/IB2005/053162 patent/WO2006033088A2/en active Application Filing
- 2005-09-23 CA CA2581087A patent/CA2581087C/en not_active Expired - Fee Related
- 2005-09-23 CN CNA2005800324156A patent/CN101031659A/zh active Pending
- 2005-09-23 EP EP05784740A patent/EP1815010A2/en not_active Withdrawn
- 2005-09-23 NZ NZ554538A patent/NZ554538A/en not_active IP Right Cessation
- 2005-09-23 AU AU2005286084A patent/AU2005286084C1/en not_active Ceased
- 2005-09-23 US US11/575,821 patent/US8486622B2/en not_active Expired - Fee Related
- 2005-09-23 JP JP2007533053A patent/JP2008514197A/ja active Pending
- 2005-09-23 SG SG200906440-3A patent/SG155995A1/en unknown
- 2005-09-23 BR BRPI0516955-0A patent/BRPI0516955A/pt not_active IP Right Cessation
- 2005-09-23 KR KR1020077009132A patent/KR20070062575A/ko not_active Ceased
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2007
- 2007-03-21 IL IL182107A patent/IL182107A0/en unknown
- 2007-04-24 ZA ZA200703326A patent/ZA200703326B/xx unknown
Also Published As
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CN101031659A (zh) | 2007-09-05 |
NZ554538A (en) | 2009-12-24 |
AU2005286084C1 (en) | 2011-11-24 |
ZA200703326B (en) | 2008-11-26 |
RU2007115421A (ru) | 2008-10-27 |
JP2008514197A (ja) | 2008-05-08 |
US20090123914A1 (en) | 2009-05-14 |
CA2581087C (en) | 2013-11-19 |
US8486622B2 (en) | 2013-07-16 |
KR20070062575A (ko) | 2007-06-15 |
WO2006033088A3 (en) | 2006-07-06 |
AU2005286084B2 (en) | 2011-04-28 |
SG155995A1 (en) | 2009-10-29 |
IL182107A0 (en) | 2007-07-24 |
RU2464320C2 (ru) | 2012-10-20 |
WO2006033088A2 (en) | 2006-03-30 |
EP1815010A2 (en) | 2007-08-08 |
AU2005286084A1 (en) | 2006-03-30 |
CA2581087A1 (en) | 2006-03-30 |
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