This study reports the biochemical profile and in vitro biological activities of the aerial part ... more This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by determining its physiological properties and approximate composition. Hydro-methanolic extracts from Halocnemum strobilaceum and Suaeda fruticosa have been investigated for the inhibition of bacterial growth, the protection of proteins (albumin) from denaturation, and cytotoxicity to hepatocellular carcinomas (Huh-7 and HepG2). Their antioxidant activity was evaluated by five tests, including one that examined their ability to inhibit hydrogen peroxide (H2O2)-induced hemolysis. The profile of their phenolic compounds was also determined. These two euhalophytes had a high moisture content, high levels of photosynthetic pigments, elevated levels of ash and protein, low oxidative damage indices, MDA (Malondialdehyde) and proline, and low lipids levels. Th...
Evidence-Based Complementary and Alternative Medicine, 2021
Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histo... more Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks show...
Nanotechnology nowadays has good potential application in many fields, especially in agriculture ... more Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by eco-friendly method from silver nitrate and water extract of Pulicaria undulate on seed germination of two weeds species. Aqueous extract and silver Nanoparticles plant extract (AgNPs) had an inhibitory effect on seed germination of common (Lepidium sativum, and Trifolium repens) as compared with control. Lpidum stavium seed germination was inhibited by nanoparticle and water plant extract at 100% concentration at the end of 10 day incubation period, at low concentration 25% of aqueous extract and AgNPs the germination ratio of L. sativium reached (30% and 5%) where for Trifolium repens the germination ratio were (50% and 0% respectively). Nanoparticle are green synthesis by simple methods, using the aqueous extract of pulclaria leave and silver nitrate,...
Drought stress and drastic condition of northern region especial high temperature in summer seaso... more Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive path...
Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2... more Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin 4'-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside (S4), were isolated from the leaves of Cupressus sempervirens. Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1H NMR, 13C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.
In this study, Thymus numidicus Poir. plant material was collected from two different locations i... more In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 an...
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics ... more As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 4.65, 12.60 and 17.90 µg/ml, respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC50 = 3.64, 4.57 and 2.97 µg/ml, respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids; identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 3-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its’ spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitro cytotoxicity against HCT116 cell line with IC50 = 20.1, 24.3, 22.8, 23.4 and 41.9 µg/ml as d...
Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical... more Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid compound isolated from terrestrial Saudi plants, the MeOH extract of the aerial parts of the plant lactuca steriolla family Asteraceae was in vitro investigated for cytotoxicity against HCT116, A549, HepG2 and MCF-7 cell lines, and resulted in vitro show high cytotoxic activity against breast cancer with for MeOH extract and good cytotoxicity against liver cancer of hexane extract, respectively, Organic extracts , were fractionated through classic chromatography. The steroids stigmasterol acetate, β-sitosterol and campesterol were identified in hexane extract. Triterpenes, germincol, lupeol, α-amyrin, β-amyrin, olean 18-ene, lupeol acetate were isolated from methanol extract. Steroids and 14b-perganane and 4- piprdenone were isolated from methanol extrac...
Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient tim... more Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient times by several medicinal systems, including those of China, India, Greece, and Egypt. Although C. sativa is one of the most investigated medicinal plants in the world, it faces some of the greatest controversies surrounding its legalization and use as a medication. C. sativa contains several hundred phytoconstituents, including the infamous “cannabinoids”. It is necessary to properly understand the medicinal importance of these phytochemicals and spread awareness among the countries where cannabis is still facing legal obstacles. The current review focuses on the most recent literature pertaining to various applications of cannabinoids, with a special focus on the medicinal aspect of these phytochemicals. Peer-reviewed articles focusing on the importance of cannabis and cannabinoids are the target of this review. Articles were selected based on the relevance to the general scope of the wo...
Synthesis of nanoparticles is a fast-growing area of interest in the current development in scien... more Synthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeru...
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 13, 2018
Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising an... more Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising antitumor compound, having exerted activity against a number of human cancer cell lines. Honokiol has inhibitory role on the proliferation, invasion and survival of cancer cells in in vitro as well as in vivo studies. It interferes with signaling pathways components in order to elicit the anticancer effect. In present review, the published data on the efficacy of honokiol against various cancer cell lines and tumor-bearing animal models has been presented and discussed. Honokiol lowers the expression of pluripotency-factors, the formation of mammosphere, P-glycoprotein expression, receptor CXCR4 level, c-FLIP, steroid receptor coactivator-3 (SRC-3), Twist1, matrix metalloproteinases, class I histone deacetylases, H3K27 methyltransferase among numerous other anticancer functions. It increases bone morphogenetic protein 7 (BMP7), Bax protein, among others. It does so by interfering with the ...
This study reports the biochemical profile and in vitro biological activities of the aerial part ... more This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by determining its physiological properties and approximate composition. Hydro-methanolic extracts from Halocnemum strobilaceum and Suaeda fruticosa have been investigated for the inhibition of bacterial growth, the protection of proteins (albumin) from denaturation, and cytotoxicity to hepatocellular carcinomas (Huh-7 and HepG2). Their antioxidant activity was evaluated by five tests, including one that examined their ability to inhibit hydrogen peroxide (H2O2)-induced hemolysis. The profile of their phenolic compounds was also determined. These two euhalophytes had a high moisture content, high levels of photosynthetic pigments, elevated levels of ash and protein, low oxidative damage indices, MDA (Malondialdehyde) and proline, and low lipids levels. Th...
Evidence-Based Complementary and Alternative Medicine, 2021
Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histo... more Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks show...
Nanotechnology nowadays has good potential application in many fields, especially in agriculture ... more Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by eco-friendly method from silver nitrate and water extract of Pulicaria undulate on seed germination of two weeds species. Aqueous extract and silver Nanoparticles plant extract (AgNPs) had an inhibitory effect on seed germination of common (Lepidium sativum, and Trifolium repens) as compared with control. Lpidum stavium seed germination was inhibited by nanoparticle and water plant extract at 100% concentration at the end of 10 day incubation period, at low concentration 25% of aqueous extract and AgNPs the germination ratio of L. sativium reached (30% and 5%) where for Trifolium repens the germination ratio were (50% and 0% respectively). Nanoparticle are green synthesis by simple methods, using the aqueous extract of pulclaria leave and silver nitrate,...
Drought stress and drastic condition of northern region especial high temperature in summer seaso... more Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive path...
Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2... more Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin 4'-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside (S4), were isolated from the leaves of Cupressus sempervirens. Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1H NMR, 13C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.
In this study, Thymus numidicus Poir. plant material was collected from two different locations i... more In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 an...
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics ... more As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 4.65, 12.60 and 17.90 µg/ml, respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC50 = 3.64, 4.57 and 2.97 µg/ml, respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids; identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 3-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its’ spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitro cytotoxicity against HCT116 cell line with IC50 = 20.1, 24.3, 22.8, 23.4 and 41.9 µg/ml as d...
Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical... more Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid compound isolated from terrestrial Saudi plants, the MeOH extract of the aerial parts of the plant lactuca steriolla family Asteraceae was in vitro investigated for cytotoxicity against HCT116, A549, HepG2 and MCF-7 cell lines, and resulted in vitro show high cytotoxic activity against breast cancer with for MeOH extract and good cytotoxicity against liver cancer of hexane extract, respectively, Organic extracts , were fractionated through classic chromatography. The steroids stigmasterol acetate, β-sitosterol and campesterol were identified in hexane extract. Triterpenes, germincol, lupeol, α-amyrin, β-amyrin, olean 18-ene, lupeol acetate were isolated from methanol extract. Steroids and 14b-perganane and 4- piprdenone were isolated from methanol extrac...
Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient tim... more Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient times by several medicinal systems, including those of China, India, Greece, and Egypt. Although C. sativa is one of the most investigated medicinal plants in the world, it faces some of the greatest controversies surrounding its legalization and use as a medication. C. sativa contains several hundred phytoconstituents, including the infamous “cannabinoids”. It is necessary to properly understand the medicinal importance of these phytochemicals and spread awareness among the countries where cannabis is still facing legal obstacles. The current review focuses on the most recent literature pertaining to various applications of cannabinoids, with a special focus on the medicinal aspect of these phytochemicals. Peer-reviewed articles focusing on the importance of cannabis and cannabinoids are the target of this review. Articles were selected based on the relevance to the general scope of the wo...
Synthesis of nanoparticles is a fast-growing area of interest in the current development in scien... more Synthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeru...
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 13, 2018
Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising an... more Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising antitumor compound, having exerted activity against a number of human cancer cell lines. Honokiol has inhibitory role on the proliferation, invasion and survival of cancer cells in in vitro as well as in vivo studies. It interferes with signaling pathways components in order to elicit the anticancer effect. In present review, the published data on the efficacy of honokiol against various cancer cell lines and tumor-bearing animal models has been presented and discussed. Honokiol lowers the expression of pluripotency-factors, the formation of mammosphere, P-glycoprotein expression, receptor CXCR4 level, c-FLIP, steroid receptor coactivator-3 (SRC-3), Twist1, matrix metalloproteinases, class I histone deacetylases, H3K27 methyltransferase among numerous other anticancer functions. It increases bone morphogenetic protein 7 (BMP7), Bax protein, among others. It does so by interfering with the ...
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